PCR을 이용한 국내시장에 유통중인 유전자재조합 콩 및 가공식품의 모니터링

김묘영,김재환,김현중,박선희,우건조,김해영 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.4

본 연구에서는 PCR을 이용하여 국내시장에 유통중인 원료콩과 가공식품에 epsps 또는 pat 유전자가 삽입된 유전자재조합 콩(GMS)의 사용여부를 모니터링하였다. 이러한 GMS의 검출을 위해 3쌍의 primer set을 제작하였고, 각각의 primer들은 GMS에 삽입된 유전자와 특이적으로 반응하여 PCR산물을 생성하였다. 2001년 표시제가 시행되기 이전에 생산된 총 가공식품과 이후의 제품에 대해 각각 모니터링을 수행하였으며, 표시제 이전에 생산된 제품의 경우 대부분의 미국산 원료에서 esps가 삽입된 GMS가 검출되었으나, 표시제 이후에는 검출되지 않았다. A method using PCR was developed for the monitoring of genetically modified soybean (GMS) and GMS derived foods utilized in the market. We designed 3 pairs of specific oligonucleotide primers based on epsps and pat inserted in GMS and ferritin gene as internal standards. Template DNAs isolated from soybean and processed foods were used for multiplex PCR with 3 primer sets. PCR, used with specific primer sets for GMS detection, showed the amplified DNA fragments with GMS template DNA. In this study, GMS containing epsps was detected from soy processed foods manufactured before GM food labeling system, however, GMS containing epsps or pat was not detected from soy processed foods manufactured after GM food labeling system.

人蔘多糖類의 理化學的 特性에 關한 硏究 : 人蔘貯藏加工中 澱粉의 理化學的 特性變化 Change of physicochemical properties of the starch during storage and heat treatment

曺哉銑,吳成基,曺羊嬉,金海中,黃明浩 경희대학교 부설 식량자원개발연구소 1985 硏究論文集 Vol.6 No.-

In order to investigate the change of physicochemical properties of ginseng root starch during storage and heat treatment. the roots were stored for 15 days at 5℃, 15℃, 30℃ and 45℃, and heated for 15 hours at 60℃, 70℃, 80℃, 90℃, respectively. The starch content was decreased from about 40% to 23-26% and sucrose content was increased from 4% to 12-16% during storage for 15 days at 5-45℃. Maltose, which was not detected in fresh samples, was increased upto 8.5% during storage or heat treatment. Granular size of the starch was decreased and some of the granules were broken during storage. Amylose content in the starch was decreased from 33% to 20%, and blue value and alkali number of the starch were increased slightly, and solubility and swelling power of the starch were decreased during storage. The higher storage temperature and the longer storage time, the starch was more susceptible to gelatinize, and the viscosity of the starch was lowered with the susceptibility of gelatinization. The susceptibility of degradation of the starch by the amylase was increased and amylolytic activities in ginseng root were, also, increased during storage.

가토안의 Acid Phosphatase의 활성 및 분포

김태조,손해숙,송미영,이양진 曉星女子大學校 藥學大學 學生會 1990 曉星藥誌 Vol.5 No.-

Lysosomal enzyme activity of choroid, newrosensory retina, iris and ciliarybody, cornea and sclea of the rabbit eye was measured by acid phosphatase(ASPase)as enzyme maker. We reported lysosomal enzyme activity on retinal pigment epitherium in 1998. Distribution of ACPase in the tissues was carried out using Fukuzawa method and Kind-king method. Of the tissues of the rabbit eye the ACPase activity was highest in the choroid(28.6583±12.6920)by Kind-king method. The meurosensory retina(4.5433±2.2091), Iris & ciliary body(2.4138±0.8307), cornea(1.7223±0.6492)and sclea(0.9550±0.0646)also showed the specipic activity of enzyme.

청소년의 스마트폰 중독과 우울 및 불안과의 관계

김경은,김필송,민주연,박수경,신서인,이지은,정해인,조호정,최정원,최정인 이화여자대학교 간호과학대학 2013 이화간호학회지 Vol.- No.47

Purpose: The purpose of this study was to investigate the relationships between the degree of smart phone addiction among adolescents and their depression and anxiety levels. Method: In this study, 379 high school students were selected using the convenient sampling method. The instruments used for this study were smart phone addiction measure, the CES-D (Center for Epidemiological Studies-Depression Scale), and the STAI (State-Trait Anxiety Inventory). Data were analyzed using the SPSS 20.0 program with descriptive statistics, t-test, ANOVA with Scheffe test, and Pearson’s correlation coefficient. Result: The mean score for smart phone addiction was 40.45±17.27, depression was 36.56±9.58, state anxiety was 44.00±9.61, and trait anxiety was 45.70±9.75. There were significant differences between the degree of smart phone addiction and the following variables: gender (t=-4.953, p<.001), hours of smart phone use per day (F=12.259, p<.001), types of frequently used features (F=3.485, p=.008), and satisfaction level for smart phone (F=5.18, p<.001). There were statistically significant relationships (p<.001) among degree of smart phone addiction, depression level, and state and trait anxiety levels. Conclusion: The results of this study suggested that there was a significant relationship between smart phone addiction and mental health, specifically, depression and anxiety among adolescents. Further research is needed to develop nursing strategies to provide care for adolescents who frequently utilize smart phones.

[논문]보육교사의 보육컨설팅에 대한 인식, 요구도 및 활성화 방안

황해익,조준오,김병만 부산대학교 교육연구소 2012 교육혁신연구 Vol.22 No.1

The purpose of this study was to examine the awareness of childcare teachers on childcare consulting, their needs for that and ways of revitalizing childcare consulting in a bid to lend professional assistance to childcare centers in terms of how to boost the quality of childcare. And it's also meant to provide some information on how to improve childcare consulting both in theory and practice. The subjects in this study were 200 childcare teachers in the city of Busan. The findings of the study were as follows: First, as a result of analyzing their awareness of childcare consulting, 147 respondents (87.5%) considered that to be necessary or very necessary. As for the reason for the necessity of childcare consulting, the biggest group that numbered 62 responded (42.2%) that it would be necessary to pinpoint and resolve problems. Concerning the reason why it's not necessary, the largest group that numbered four answered (19.1%) that there was no problem about which childcare consulting would be required. Second, as a result of checking their needs for childcare consulting, the biggest group that numbered 35 asked (20.8%) for education about accreditation indicators in terms of the content of childcare consulting. Regarding childcare consultants, the biggest group that numbered 55 preferred (32.7%) professional assistants, lecturers and professional personnels of childcare centers. Concerning the term of childcare consulting, the largest group that numbered 71 considered (42.3%) it advisable to receive this consulting for less than a week. As to the time for childcare consulting, the biggest group that numbered 58 answered (34.5%) that it should be provided at the request of accredited childcare centers. Third, as a result of analyzing their opinions on how to revitalize childcare consulting, the biggest group that numbered 128 felt (76.2%) the need for a separate consulting institution. The largest group that numbered 30 thought (23.4%) that the Ministry of Health and Welfare should be responsible for that, and the greatest group that numbered 76 thought (45.2%) that the top priority for childcare consulting would be to secure professional childcare consultants. 본 연구는 보육교사의 보육컨설팅에 대한 인식과 요구도 및 활성화 방안을 확인하여 어린이집의 개별적인 질적 수준 관리 및 지속적인 질 향상에 전문적이고 체계적인 도움을 제공하고, 향후 보육컨설팅의 개념적․실제적 측면을 보완하는 데 기초자료로 활용하는 것에 연구의 목적이 있다. 본 연구는 부산광역시에서 근무하는 보육교사 200명을 연구대상으로 선정하였고, 회수된 설문지 중 총 168부를 최종 분석대상으로 선정하였다. 연구결과를 살펴보면 첫째, 보육컨설팅에 대한 인식을 살펴본 결과 보육컨설팅이 ‘필요하다’와 ‘매우 필요하다’가 총 147명(87.5%)으로 높게 나타났고, 필요한 이유로는 ‘문제진단 및 현안해결’이 62명(42.2%), 불필요한 이유는 ‘보육컨설팅을 필요로 하는 문제가 없기 때문에’가 4명(19.1%)으로 가장 높게 나타났다. 둘째, 보육컨설팅에 대한 요구도를 살펴본 결과 보육컨설팅 내용에 서는 ‘평가인증 지표 교육’이 35명(20.8%), 대상은 ‘조력전문가, 강의, 센터전문요원’이 55명(32.7%), 보육 컨설팅 실시기간은 ‘1주일 미만’이 71명(42.3%), 보육컨설팅 실시 시기는 ‘평가인증을 통과한 어린이집의 요청 시’가 58명(34.5%), 보육컨설팅 지도방법은 ‘원장, 교사 상호작용 및 의견 교환’이 69명(41.1%)으로 가장 높게 나타났다. 셋째, 보육컨설팅에 대한 활성화 방안을 살펴본 결과 전담기관의 필요성은 ‘필요하다’가 128명(76.2%), 전담부서는 ‘보건복지부’가 30명(23.4%), 보육컨설팅 활성화를 위해 가장 필요한 과제는 ‘전문성을 갖춘 보육컨설턴트의 확보’가 76명(45.2%)으로 가장 높게 나타났다. 끝으로 본 연구에 서는 보육교사의 보육컨설팅에 대한 인식과 요구도 그리고 활성화 방안을 토대로 중요성과 필요성을 논의하였다.

문제중심학습(Problem Based Learning; PBL) 패키지 개발 : 지각·조정장애상황을 중심으로

김애리,김영경,송영선,신경림,안혜정,이지순,조계화 성인간호학회 2001 성인간호학회지 Vol.13 No.3

The Purpose of this study is to present an actual example for procedures for developing a PBL package based on philosophical backgrounds derived from Problem-based learning. To perform a systemic study on the operations of an intergrated curricula under multi-disciplines, a research team made up of several professors with different academic backgrounds was formed. Among the four situations for the patients with perception-adjustment disorder, especially a procedure for the development of PBL package which can be used in the emergency room situation has been proposed. The little(2000)'s PBL package model has been applied for this study. Tha package includes course objectives, learning objectives, concept map, situation scenario. tutor guide, and evaluation method. It is believed that learning objectives achievement procedures designed as a part of a problem-based learning package development procedures for the nursing of patients with perception-adjustment can be achieved at the same level as the learning objectives for the science of nursing founded by the Korean Nurses Association.

高麗人蔘(Panax ginseng C.A.Meyer) 澱粉의 理化學的 特性에 관한 硏究 : 제3보 澱粉의 物理的 特性 3. Physical properties of the starch

金海中,曺哉銑 경희대학교 부설 식량자원개발연구소 1984 硏究論文集 Vol.5 No.-

人蔘澱粉의 物理的 特性을 고찰한 결과는 다음과 같다. 1. 人蔘澱粉을 90℃로 가열하면 용해도는 약 15%이고 팽윤력은 30% 전후로써 연근별로 큰 차이가 없었으며, 흡수율은 160%였다. 2. 人蔘澱粉의 호화개시온도는 현미경관찰, X-선소실, α-amylase 소화시험 및 amylograph 분석시험 등으로 볼때 50~55℃로 시작하여 65~70℃에서 완전 호화되었다. 3. 人蔘전분과 amylose의 고유점도는 0.54 및 1.5~1.9였고, 상대점도는 0.3%농도의 경우 전분 1.3~1.5, amylose 1.6~2.9, amylopectin은 1.4~2.3이었다. 4. 전분현탁액이 농도가 증가할수록 gel의 경도와 탄력성이 증가하였으며 pH5~6에서 55분 가열했을때 최고경도를 나타냈다. 전분의 노화는 다른 전분과 같이 전분겔의 Avrami지수는 5℃ 30℃ 저장시 1,005와 0.965로 1에 가까우므로 전분노화가 instantaneous nucleation으로 진행됨을 알 수 있었다. Solubility and degree of swelling of the starch were about 15% and 30%, respectively when the starch in aqueous solution was heated at 90℃ for 30minutes. The starch showed no significant differences in the degree of swelling and soblubility with a growing period of ginseng. The starch was begun gelatinize at 50-55℃ and completed at 65-70℃ by the analytical methods of X-ray diffraction, disappearance of crystalinity. Brabender angly lographic analysis and amylase digestion test. The maximum and minimum peak viscosities of 10% starch solution were 5.500 B.U and 1960 B.U. respectively. The starch showed no significant differences in pasting temperature, maximum and minimum viscosities on amylogram with a growing period of ginseng. The intrinsic viscosities of the starch and amylose were 0.54 and 1.5-1.9, respectively.

Pseudo-Gitelman씨 증후군 1예

김성렬,조영일,이해운,이정연,안해련,송종오,명승운 건국대학교 의과학연구소 2002 건국의과학학술지 Vol.12 No.-

Gitelman's syndrome is a rare disorder with a characteristic set of metabolic abnormalities. These include hypokalemia, metabolic alkalosis, hypocalciuria and hypomagnesemia. The clinical manifestations of Gitelman's syndrome are very similar with those of Bartter's syndrome and diuretic abuse. Gitelman' syndrome and Bartter's syndrome can be distinguished by renal clearance study after administration of furosemide and thiazide, since the primary defect in each of these disorders is an impairment in sodium reabsorption in the distal tubule and loop of Henle, respectively. Pseudo-Bartter's syndrome due to diuretic abuse can be distinguished by history of diuretic use and a positive urine assay for diuretics. We have experienced a case of pseudo-Gitelman's syndrome in 56-year old woman with unexplained hypokalemic metabolic alkalosis, hypocalciuria, and hypomagnesemia. The patient denied ingestion of diuretic medication. The results of renal clearance study using furosemide and thiazide in this case suggested the presence of the primary defect of sodium reabsorption in the distal tubule rather than in the loop of Henle. These clinical and laboratory findings were consistent with those of Gitelman's syndrome. But, this case was confirmed as pseudo-Gitelman's syndrome since diuretics were detected by a urine assay for diuretics. Furosemide and hydrochlorothiazide were contained in the pill that she had been taking due to constipation. Herein, we report the case of pseudo-Gitelman's syndrome by abuse of cathartics containig diuretics presented as Gitelman's syndrome in renal clearance study.

단체급식소에서 제공되는 음식온도의 기호성에 관한 연구

金蕙英,趙允旋 성신여자대학교 생활문화연구소 1993 生活文化硏究 Vol.7 No.-

이동 열원을 고려한 전자빔 용접의 유한요소해석

조해용,정석영,김명한,조창용,이제훈,서정 한국레이저가공학회 2001 한국레이저가공학회지 Vol.4 No.1

Simulation on the electron beam welding of Al 2219 alloy was carried out by using commercial FEM code MARC, which encounters moving heat sources. Due to axisymmetry of geometry, a half of the cylinder was simulated. A coupled thermo-mechanical analysis was carried out and subroutine for heat flux was substituted in the program. The material properties such as specific heat, heat transfer coefficient and thermal expansion coefficient were given as a function of temperature and the latent heat associated with a given temperature range is considered. As a result, the proper beam power is 60kVX60mA and welding speed is 1~1.5 m/min. The residual stress in the heat-affected zone as well as the fusion zone does not increase. It is necessary to use jigs for preventing distortion of cylinder and improving weld quality.