PCR을 이용한 국내시장에 유통중인 유전자재조합 콩 및 가공식품의 모니터링

김묘영,김재환,김현중,박선희,우건조,김해영 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.4

본 연구에서는 PCR을 이용하여 국내시장에 유통중인 원료콩과 가공식품에 epsps 또는 pat 유전자가 삽입된 유전자재조합 콩(GMS)의 사용여부를 모니터링하였다. 이러한 GMS의 검출을 위해 3쌍의 primer set을 제작하였고, 각각의 primer들은 GMS에 삽입된 유전자와 특이적으로 반응하여 PCR산물을 생성하였다. 2001년 표시제가 시행되기 이전에 생산된 총 가공식품과 이후의 제품에 대해 각각 모니터링을 수행하였으며, 표시제 이전에 생산된 제품의 경우 대부분의 미국산 원료에서 esps가 삽입된 GMS가 검출되었으나, 표시제 이후에는 검출되지 않았다. A method using PCR was developed for the monitoring of genetically modified soybean (GMS) and GMS derived foods utilized in the market. We designed 3 pairs of specific oligonucleotide primers based on epsps and pat inserted in GMS and ferritin gene as internal standards. Template DNAs isolated from soybean and processed foods were used for multiplex PCR with 3 primer sets. PCR, used with specific primer sets for GMS detection, showed the amplified DNA fragments with GMS template DNA. In this study, GMS containing epsps was detected from soy processed foods manufactured before GM food labeling system, however, GMS containing epsps or pat was not detected from soy processed foods manufactured after GM food labeling system.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

신체형 환자의 애착 특성

김용희,송지영,반건호,김종우,신용선 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The purpose of this study was to evaluate the relationship betseen attachment style and somatization in patients with somatoform disorder. Methods : Data was collected by self-report questionnaires. 134 normal subjects and 30 patients with somatoform disorder were enrolled in this study. All subjects completed a psychometric assessment that consisted the Revised Adults Attachment Scale(RAAS), Inventory of Parent and Peer Attachment (IPPA), Sungshin Self-concept Inventory (SSCI), and SCL-90-R. Results : 1) Somatoform patients showed more anxious attachment style than normal control. 2) Anxious attachments were combined with mistrust of their parents, emotional instability, and interpersonal mistrust which may play a significant portion of variance in somatization. Conclusion : We found that patients with somatoform disorder had more anxious attachment style than normal subjects. Insecure attachment patterns may significantly explain the phenomenon of somatization.

凍結鷄肉의 品質變化에 關한 硏究 : Ⅳ. 凍結 冷藏時의 理化學的 變化

金建培,李根雨 군산대학교 1986 群山水産專門大學 硏究報告 Vol.20 No.3

The quality variation of the frozen chicken meat pretreated with the various methods were studied during the cold storage. For the purpose of this study, the frozen chickens as samples were divided into five groups, T1 group (untreated), T2 group (treated with 1% ascorbic acid), T3 group (7.5% potassium sorbate), T4 group (5% NaCl) and T5 group (mixed with 7.5% potassium sorbate and 1% ascorbic acid). All samples were kept the cold storage for 90 days, the item of investigation on the thawed samples were pH, water holding capacity, changes of drip, P.O.V. and T.B.A. The values of pH in T3, T4 and T5 groups were continuously increased according to the storage days, but those in T1 and T2 groups were decreased after 60 days (p〈0.01). The water-folding capacity was the lowest (66.22%) in T, group and the higest (70.72%) in T5 group, and the relatively stable in T4 and T5 groups. The free drip level was the highest as 5.15% in T1 group and the lowest as 1.6% in T5 group, and the expressible drip was relatively little in T4 and T5 groups compared with other groups (p〉0.05). The value of P.O.V in T1 group was the highest as 10.2 meq/Kg and the lowest as 4.7 meq/Kg in T5 group (p〈0.01). The value of T.B.A. was also the highest as 0.507 in T1 group and the lowest as 0.232 in T5 group (p〉0.05). The store ability of the frozen chicken meat was good in T5 group.

정신분열증 입원환자에서 니코틴과 항정신병 약물의 상관관계 규명을 위한 니코틴 패취 적용 연구

반건호,유희정,윤도준,김종우,이기철 대한생물치료정신의학회 2000 생물치료정신의학 Vol.6 No.1

연구목적 : 정신분열증 환자는 흡연율이 높고, 그에 따라 항정신병 약물 투여량이 늘어나고, 약물에 의한부작용은 오히려 감소한다고 알려져 있다. 이러한 연구에서 방법론적 문제로는 실제 니코틴 용량을 측정하지 않았다는 점이다. 본 연구는 담배보다 니코틴의 주요 대사물인 코티닌을 측정하여 방법론적 오류를 최소화하고자 하였다. 방법 : 정신분열증으로 진단받은 입원환자 중 니코틴 패취군 8명, 대조군 8명을 설정하였다. 5일간 패취를 붙인 후 혈액에서 코티닌을 측정하였고, 정신증상의 정도를 알기 위하여 PANSS, 약물의 추체외로 부작용 측정을 위하여 ESRS를 실시하였다 일일 약물용량을 chlorpromazine 동량으로 환산하여 기록하였다. 결과 : 코티닌 수치와 정신증상, 추체외로부작용, 약물용량 사이에는 유의한 차이가 없었다. 결론 : 코티닌은 항정신병 약물의 작용에 영향을 미치지 못하였다. 단, 향후 연구에서는 니코틴 패취의 양을 증량하여 사용하므로써, 코티닌 용량에 따라 항정신병 약물효과에 영향을 미칠 수 있는지를 검증해야 할 것이다.

Diamond-Like Carbon 코팅을 이용한 금속 장식 용구의 칼라링 및 디자인연구

변건호,김형준,최형우,권도현 한국공예학회 2001 조형디자인연구 Vol.4 No.2

The usual methods of coloring metal ornaments include surface anodizing, plating and painting. However, the anodizing method can apply to AL materials only and the plating method has been causing a severe environmental problem because of the toxicity of materials used. For these methods, surface deteriorations such as decoloration, scratch and corrosion also occur after long-time use. Therefore, a better surface treatment method is needed. This study was conducted to improve corrosion resistance and wear resistance of metal ornaments, and increase their added value by means of the DLC(Diamond-Like-Carbon) coating method, and to expand the range of its application. This study examined the characteristics of the DLC for coloring metal ornaments and the ornamental design for practical application. Coating metal ornaments could be considered as one of promising fields for application of DLC DLC film is very suitable as a coating material because it is an amorphous carbon material based on covalent bond and therefore has excellent hardness, wear resistance, lubricative property, chemical stability and light transmittance. DLC coating film was formed by the PECVD method using 13.56MHz rf plasma and CH4 gas as a material gas. At this time RF power, working pressure, transmittance according to kind and amount of auxiliary gas, and changes in optical band gap were observed. It was shown that the higher the RF power and working pressure, the lower the optical bang gap. This could be demonstrated by observing the amount of carbon-hydrogen bond by means of FT-IR. It was shown that addition of hydrogen and nitrogen would the optical band gap because they serves to break the carbon-hydrogen bond at the time of vapor deposition. The direction of design was set up in the manner that the DLC coating film could be effectively deposited on the surface of an ornament. Design was based on geometric figures and minimalism with plane elements accentuated. Accentuated, partial coating or simple, whole coating was induced by combining quadrangles, triangles and circles basically.

병식 평가 척도-한국판(SUMD-K)의 신뢰도와 타당도 검증

송지영,김기태,이서경,김용희,노준호,김종우,장환일,반건호,강원섭 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.4

Objectives : This study was conducted to evaluate the reliability and the validity of the Korean version of the Scale to Assessment Unawareness of Mental Disorder (SUMD-K). Methods : The subjects were 55 schizophrenic patients between ages of 26 and 58 who were recruited from the psychiatric unit of the university hospital and the out-patient clinic. The SUMD-K and insight scale of PANSS were applied to the patients. Results : The range of inter-rater (Spearman's rho) of the general items of SUMD-K were .97-.98 on the current illness and .58-.98 on the past illness. The correlation coefficient between the sum and the general items of the SUMD-K showed high scores. The concurrent validity between SUMD-K and insight item of PANSS was high (scores between .59-.96). However,the reliability and the validity of the attribution items of symptoms showed low scores compared to the current symptoms. Conclusion : The SUMD-K was confirmed in its reliability and validity. It can be a useful clinician-rating scale for evaluating insight in schizophrenic patients as well as patients with other neuropsychiatric conditions.

미생물 포괄 고정화방식을 이용한 수산물 가공공장 폐수처리

정병철,장수현,김종배,김건배,최선남,이근우,정병곤 7개 국립대학교 환경연구논문집 공동발행 위원회 2005 공업기술연구 Vol.5 No.-

Feasibility of simultaneous removal of organic and nitrogen is evaluated as applying microbial entrapping method to treat fish processing wastewater. Treatability test is conducted immobilizing activated sludge from municipal sewage treatment plant to gel matrix made of cellulose triacetate. Stable operation can be possible when applying organic and nitrogen loading rate increasingly to wastewater treatment system composed of anoxic and oxic tank. Organic loading rate and nitrogen loading rate are applied 0.65-1.72kg COD/㎥/d and 0.119-0.317kg T-N/㎥/d, respectively. In case of wastewater from fish processing plant used in this study, it is evaluated that 0.3kg T- N/㎥/d is upper limit of applicable space loading rate because effluent T-N concentration is exceed 60mg/L, legal effluent dishargeable limit of T-N concentration in Korea, when space loading rate is exceed 0.3kg T-N/㎥/d. It is evaluated that nitrification of system is performed efficiently regardless fo variation of applied loading rate because removal efficiency of NH_(4)^(+)-N is relatively constant when the applied NH_(4)^(+)-N loading rate is increased gradually, while decrease of T-N removal efficiency is apparent when the applied loading rate is increased gradually. Nitrate removal efficiency in this study is varied from 98.62% to 99.51% depending on applied loading rate, while nitrification efficiency of oxic reactor is varied from 94.0% to 96.9%. It is showed that COD removal efficiency is varied from 94.2% to 96.6% and T-N removal efficiency is varied from 73.4% to 83.4% depending on loading rate applied in this study.

정신분열병 및 정신분열형 장애에 대한 올란자핀의 효과와 안전성

안용민,강대엽,권준수,김창윤,김철응,반건호,신영민,이기철,이동우,이중서,조현상,채정호,김용식 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.4

연구목적 : 본 다기관 공동임상연구는 국내 환자를 대상으로 새로운 항정신병약물인 올라자핀의 치료 효과와 안전성을 확인하고자 시행되었다. 방 법 : 1999년 7월부터 2000년 3월까지 국내 10개 병원에 방문한 정신분열병 및 정신분열형 장애 105명을 대상으로 비대조 개방 임상연구를 시행하였다. 중등도 이상의 정신병 증상을 지니거나, 부작용으로 약물교체가 필요한 경우 또는 기분장애 증상의 치료가 필요한 환자를 대상으로 하였다. 2∼7일간의 약물 배설기간 후에 1일 10mg의 올란자핀을 투여하였고, 이후로 3∼7일 간격으로 용량을 조절하여 총 8주간 투여하였다. 치료 효과는 PANSS(Positive and Negative Syndrome Scale), BPRS(Brief Psychiatric Rating Scale), CGI(Clinical Global Impression-Severity), MADRS(Montgomery-Asberg Depression Rating Scale), HAM-A(Hamilton Rating Scale for Anxiety)로 판정하였다. 안전성 평가는 자발적인 이상반응 보고, 활력징후와 혈액 검사 및 SAS(Simpson-Angus Scale), BARS(Barnes Akathisia Rating Scale), AIMS(Abnormal Involuntary Movement Scale)척도를 이용하였다. 결 과 : 105명중에서 85.7%인 90명이 8주간의 치료를 완료하였다. 평균 최빈 용량은 일일 16.1(±4.7)mg이었고, 종료시점에서의 BPRS전체 점수가 기저 상태에 비해 40%이상 감소된 반응군은 69.5%이었다. PANSS의 양성 증후군과 일반정신병리 뿐만 아니라 음성 증후군 소척도에서도 유의한 감소를 보였으며, 이 감소들은 치료 초기부터 나타나서 8주간 지속되었다. 43.8%의 피험자가 중등도 이상의 우울증상을 같이 지니고 있었으며, 올란자핀에 의해 MADRS와 HAM-A가 유의하게 감소하였다. 치료 기간 동안에 활력징후에는 뚜렷한 변화가 없었으나 체중은 8주간 지속적으로 증가하였다. SAS와 AIMS는 감소하였으며, 정좌불능증을 제외한 나머지 추체외로 증상의 발생비율도 낮았다. 임상적인 증상이나 징후를 동반하지 않는 ALT/SGPT의 상승을 보였지만 프로락틴을 포함하는 대부분의 혈액 검사상 뚜렷한 이상 변화는 없었다. 결 론 ; 비록 본 임상연구가 비대조 개방 임상연구로서 많은 제한점을 가지고 있지만, 올란자핀이 기존 약물에 내약성을 보이거나 기분증상을 동반하는 정신병에 치료 효과가 있었다. 그리고 기존 항정신병약물 치료시에 문제가 되었던 추체외로 증상과 고프르로락틴 혈증을 포함하는 대부분의 부작용면에서 안전성을 보여 주었다. Objective : This multicenter clinical trial was carried out to investigate the efficacy and the safety of olanzpine for the treatment of Korean patients. Mothod : 105 patients with schizophrenia and schizophreniform disorder, visited at 10 mental or university hospitals, had received an open and non-comparative treatment with olanzapine for 8 weeks. Patients had psychotic or depressive symptoms with the severity above moderate degree or intolerable side effects to previous antipsychotics. After a wash-out period of 2-7 days, 10mg olanzapine was prescribed initially to all the patients, and then the dosage could be adjusted within the range of 5-20mg/day of olanzapine by 3-7 days. Results : 90(85.7%) of 105 patients completed the 8-weeks trial and the mean modal dose of olanzapine was 16.1(±4.7%)mg/day. At the end of the trial, 73 patients(69.5%) were classified as responder, which was defined as 40% or more improvement in BPRS(Brief Psychiatric Rating Scale) score comparing to baseline. There was a significant reduction in the scores of PANSS(Positive and Negative Syndrome Scale)and subscales including negative symptom scores and CGI. Also weekly analysis showed that the reductions in scores were kept on for the whole period of the trial. 43.8% of all the patients had depressive symptoms at the baseline and total scores of MADRS(Montgomery-Asberg Depression Rating Scale) and HAM-A(Hamilton Rating Scale for Anxiety) were also reduced after the trials. Vital signs revealed no clinically significant changes but continuous weight gain was observed during the treatment with olanzapine. The scores of SAS(Simpson-Angus Scale) and AIMS(Abnormal Involuntary Movement Scale) for assessing the EPS(extrapyramidal symptoms)and tardive dyskinesia respectively were significantly decreased and only a few patients reported EPS as adverse events. Although mild and clinically non-significant of ALT/SGPT was observed, most laboratory parameters including plasma prolactin level showed to significant changes during the trial. Conclusions : Although this trial had many limitations because it was a non-comparative and open study, olanzapine showed high efficacy on the positive, negative and depressive symptoms in schizophrenia and schizophreniform disorder.In addition to that, olanzapine showed a substantially favorable safety profile, such as low incidence of EPS and hyperprolactinemia.

이기종간의 상호운용성을 지원하는 이동 에이전트 시스템

이명준,박양수,유양우,문남두,김진홍,안건태 울산대학교 2000 공학연구논문집 Vol.31 No.1

현재 대부분의 이동 에이전트 시스템들은 그들 자신의 구조를 가지며 서로 다른 방법으로 구현되어 있다. 이러한 에이전트 시스템들은 서로 다른 인터페이스를 제공하고 있어서 이기종의 에이전트 시스템에서 새성된 에이전트들은 상호 이동하여 작업을 수행할 수 없다. 이러한 문제를 해결하기 위하여 이동 에이전트 시스템간의 상호운용성 지원이 중요한 과제로 다루어지고 있다. OMG(Object Management Group)는 이질적인 에이전트 시스템간의 상호운용성을 증진시킬 목적으로 MAF(Mobile Agent Facilities)명세를 제안하였다. MAF명세는 MADAgentsystem과 MAFFinder 두개의 인터페이스로 구성되어 있으며 에니전트 관리, 코드의 이동성, 명명 규칙에 관한 기능을 제공한다. 본 논문에선는 OMG의 MAF 명세를 만족하는 SMART 이동 에이전트 시스템의 설계와 이의 구현에 대하여 설명한다 SMART 이동 에이전트 시스템의 구성은 에이전트를 실행시키는 환경을 제공하는 플레이스(place), 에이전트의 라이프사이클과 플레이스를 감시하는 모니터(monitor) 그리고 에니전트에게 시스템의 자원을 할당하는 자원관리자(resource manager)로 이루어져 있다. Most current mobile agent systems adopted their own architectures, being implemented in various ways. Since those agent systems provide different interfaces, agents created in one mobile agent system could not move to other agent systems to perform their tasks. To solve this problem, interoperability becomes one of the important issues on mobile agent systems. The OMG(Object Management Group) proposed the MAF(MAF(Mobile Agent Facilities) specification for the interoperability among heterogeneous mobile agent systems. the MAF specification contains MAF Agent System and MAFFinder interfaces, and defines agent management, code mobility and naming rules. In this paper, we describe the design and implementaion of the SMART mobile agent system which satisfies the OMG MAF specification. The SMART system consists of Places that provide execution environments for agents, Monitor that monitors life-cycle of agents and places, and Resource Manager that allocates resources of system for agent.