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      • 강원지역 중소기업의 환경경영 실태와 과제

        전영승,박은정 한국산학경영학회 2004 산학경영연구 Vol.17 No.1

        본 연구의 목적은 ISO14001 인증 취득 현황조사를 통해 강원지역 중소기업의 환경경영 실태와 과제를 파악하고 환경경영의 활성화를 위한 방안을 모색하는 것이다. 주요 결과를 요약하면 다음과 같다. 첫째, 2003년 4월 현재 우리나라의 ISO14001 인증획득 업체수는 1,215개 업체인데 비해 강원지역 중소기업의 ISO14001 인증획득 업체는 광역지방자치단체 중 최하위 수준인 26개 업체에 불과하였다. 둘째, 인증 미획득 기업들이 인증을 받지 않으려는 이유로 '인증 획득 및 유지에 소요되는 비용이 효익보다 크다'는 점을 제시하였다. 셋째, ISO14001 인증을 획득한 기업이나 ISO14001 인증 미획득 기업이나 인증을 획득한(하려는) 가장 큰 이유는 '기업의 이미지 향상'이었으며, 인증 획득기업이 환경경영시스템 도입 후 얻은 효과도 '기업의 이미지 향상'으로 나타났다. 넷째, ISO14001 인증을 획득한 기업들은 환경경영시스템을 도입할 때 문제점으로 '문서작성 및 비용에 관한 부담'과 '인력 부족'에 관한 응답이 많았다. 위와 같은 연구의 주요 결과를 바탕으로 강원지역 중소기업의 환경경영 활성화를 위한 과제와 방안을 제시하면 다음과 같다. 첫째, ISO14001 인증 미획득 기업 대부분이 ISO14001에 대한 인지도가 낮아 이에 대한 지속적이고 적극적인 홍보와 교육 및 연수제도가 필요하다는 점이다. 둘째, 환경경영 관련 인력이 부족함으로 전문 인력의 양성을 위한 교육프로그램의 실시, 보조금 지급의 확대, 상담부서 및 상담창구의 개설, 관련 정보의 데이터 베이스화나 소프트웨어의 개발이 필요하다. 셋째, 저렴한 비용과 간단한 절차를 통해 인증을 받을 수 있는 중소기업용 공적 인증제도의 신설, 혹은 단체인증제도, 공단 인증제도 등을 적극 고려할 필요가 있다. The purpose of this study is to understand the realities and the subject of environmental management for small and medium-sized companies in Gwangwon area, through surveying the present status as to acquiring the certification of ISO14001, and to seek for a plan to facilitate environmental management. Given summarizing key results, those are as follows. First, while the number of companies in our country which acquired the certification of ISO14001, amounts to 1,215 businesses as of April of 2003, the number of small and medium-sized companies in Gwangwon area which obtained the certification of ISO14001 reached only 26 businesses, the lowest level among metropolitan municipalities. Second, for the reason that companies who didn't acquire the certification, strive not to receive the certification, it did present the point that 'costs to be needed in acquiring and maintaining the certification are larger than practical benefit. Third, the biggest reason for either companies which did not acquire the certification of ISO14001 or companies which did (try to) acquire the certification of IS01400, was 'enhancement of a corporate image,' and the effect after a company who obtained the certification introduced the environmental management system, was also shown to be 'the improvement of a corporate image.' Fourth, many companies who acquired the certification of IS01400 pointed out the response related to 'burden on document creation and costs' and 'lack of manpower' as problems when introducing the environmental management system. On the basis of major results of a study as the above, given presenting the subject and a plan for activating the environmental management of small and medium-sized companies in Gwangwon area, those are as follows. First, because most of companies who did not obtain the certification of IS01400 have low recognition of ISO14001, it needs continuous and positive publicity, education and a training system. Second, it requires to carry out an educational program to nurture professional manpower due to lack of manpower relevant to environmental management, to expand payment of subsidies, to open exclusive-charge department and consulting contact, to have the relevant information be database and to develop software. Third, in order to make the certification obtained through inexpensive costs and simple procedures, it needs to positively consider the creation of public approval system for a small and medium-sized company, group approval system, industrial-complex approval system, and others.

      • 地域農業成長에 대한 空間構造와 農業特化分析 : 忠北地域을 中心으로

        全榮吉,沈銀淑 안성산업대학교 1990 論文集 Vol.22 No.-

        The objectives this study are as follows; First, It is that the determination of regional unit project in Chung Buk Area. Second, the aim of this paper is to make clear the changing processes of the activity space and the periodic market. Third, It was the analysis of agricultural specilization in each regional unit. Results of this study are as follows; Firstly, this study is based on Functional Integration Model on the agricultural growth. Secondly, the range of the activity spaces, defined by lower-ordered items such as the purchase of speciality goods, the entertainment trips, and the shipment of special agricultural product have shown a remarkable change, and the orientation of behavior have changed from lower-ordered centers to higher-ordered ones. Thirdly, the crops of radish, chinese cabbage, red paper, garlic, seasame, tobacco, ginseng, stone-leek, watermelon, strawberry, tomato, raising silkworms and fruits had location quotient higer than I in agricultural specialization analysis.

      • KCI등재

        지역4-H조직의 내적 적합성에 관한 문화기술적 연구

        김진모,김진군,전영욱,이정은,최영창,이은영 한국농업교육학회 2008 농업교육과 인적자원개발 Vol.40 No.2

        이 연구의 목적은 지역4-H조직의 내적 적합성을 탐색하고 이를 기술하는데 있었다. 2006년 8월부터 10월까지 약 3개월 동안 지역4-H후원회 회장 및 임원진 3명, 지역4-H연맹 회장 1명, 학교4-H 지도교사 4명, 지역의 영농4-H회장 1명, 시·군 농업기술센터 소장 및 4-H담당자 9명을 주요 연구 참가자로 하여 심층면담을 통해 자료를 수집하였다. 수집한 자료를 분석한 결과는 다음과 같았다. 첫째, 지역4-H조직 관련자들이 인식하는 지역4-H조직의 주요성과 격차로, ① 민간4-H조직을 중심으로 한 자율적이고 독립적인 활동 부재, ② 지역4-H조직 간 다양한 종류의 협력 부재, ③ 지역사회에서 4-H활동에 대한 가치 인식 부족이 나타났다. 둘째, 지역4-H조직의 핵심 과업으로 ① 4-H의 가치 및 이념에 부합하고, 지역의 실정에 맞는 프로그램 확보, ② 지역4-H 조직 간 협력적 네트워크 확립, ③ 4-H에 대한 지역사회의 관심 및 지지 확보가 나타났다. 셋째, 지역4-H조직의 내적 부적합성으로는 ① 인원 확보의 어려움과 핵심과업에 대한 전문성 결여, ② 관료화되고 경직된 낡은 문화, ③ 조직적 자원의 축소 및 조직 간 낮은 네트워크의 수준이 도출되었다. The purpose of this study was to describe and understand internal fit in Local 4-H Organization. The main participants of this study were one leader and two members of Local 4-H Society for the support, one leader of Local 4-H Association, four School 4-H teacher, one leader of Local farm 4-H, 9 local agricultural extension workers. The data were collected through in-depth interview with them from August, 2006 to October, 2006. The Results from this study were as follow: 1. Key performance gaps of local 4-H organizations were identified: ① lack of local 4-H based activities, ② lack of various types of partnership between local 4-H organizations, ③ lack of understanding value of 4-H activities in the communities. 2. Key tasks of local 4-H organizations were identified: ① developing local 4-H programs based on 4-H's values and ideas, ② establishing the cooperative networks among local 4-H organizations, ③ ensuring the interest and support about 4-H in the communities. 3. Internal misalignments of local 4-H organizations were identified: ① difference of recruitment and lack of expertise, ② bureaucratic and rigid cultures, ③ decrease in organizational support and low level of network among organizations.

      • KCI등재

        Burkholderia cepacia를 이용한 축산폐수의 처리

        정팔진,조선영,현미희,김민정,이은주,전민식 한국물환경학회 2004 한국물환경학회지 Vol.20 No.6

        We found that the Bacterium Burkholderia cepacia in livestock wastewater treatment plant was predominant species. We investigated the growth rate of this and treatment characteristics for organic matter and nitrogen removal in livestock wastewater using this microorganism. First, we cultured B. cepacia. And then, to conducted treatment for livestock wastewater by using B. cepacia., we changed C/N from 0.2~4.4. When we operated A and B process, changing F/M ratio from 1.2 to 4.4. In experiment of C/N variations, when C/N was 1.8, we found that the optimal condition for organic matter and nutrient removal effect was higher and the removal efficiency of SCOD_(cr), SBOD_5, NH₄-N was 78.4%, 95% and 74.8%. So, It is possible to treat the wastewater having the lower C/N contents such as livestock wastewater using this microorganism. In experiment of A and B process for livestock wastewater, we found that the removal efficiency of organic matter and nitrogen in operating mode of A process was higher than that of B process. Also, the optimal F/M operating A process was 0.013 and the removal efficiency of SBOD_5, SCOD_(cr), TN and TP were 97%, 60%, 95% and 91%.

      • n-CdS_(0.46)Se_(0.54)/p-Cu_92-x)S_(0.46)Se_(0.54) 이종접합 태양전지의 제작과 그 특성에 관한 연구

        유상하,최승평,이상열,홍광준,서상석,김혜숙,전승룡,윤은희,문종대,신영진,정태수,신현길,김택성,유기수 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

        승화방법에 의해 CdS_0.46Se_0.54 단결정을 성장하여 결정구조를 조사하고, Van der Pauw 방법으로 Hall effect를 측정하여 carrier density의 온도 의존성과 mobility의 온도 의존성을 조사하였다. 성장된 CdS_0.46Se_0.54 단결정을 치환반응하여 n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 이종접합 태양전지를 제작하였다. Spectral response, 전류-전압특성 및 전력변환 효율을 조사하여 그 결과로부터 개방전압은 0.48V, 단락 전류 밀도는 21mA/㎠, fill factor와 전력변환효율은 각각 0.75와 9.5%를 얻었다. CdS_0.46Se_0.54 single crystal was grown by a sublimation method. The crystal structure and the temperature dependence of carrier density and mobility of CdS_0.46Se_0.54 single crystal were studied. Heterojunction solar cells on n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 were fabricated by the substitution reaction. The spectral response, the J-U characteristics and the conversion efficiency of the n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 heterojunction solar cells were studied. The open-cricuit voltage, short-circuit density, fill factor and conversion efficiency of n-CdS_0.46Se_0.54/p-Cu_2-xS_0.46Se_0.54 heterojunction solar cells under 80mW/㎠ illumination were found to be 0.48V, 21mA/㎠, 0.75 and 9.5%, respectively.

      • KCI등재

        Desmin Binding Property of Nebulin Isoforms

        Jeon Eun-Hee,Lee Yeong-Mi,Lee Min-A,Kim Ji-Hee,Choi Jae-Kyong,Park Eun-Ran,Kim Hyun-Suk,Ahn Seung-Ju,Min Byung-In,Joo Young-Mi,Kim Chong-Rak The Korean Society for Biomedical Laboratory Scien 2006 Journal of biomedical laboratory sciences Vol.12 No.2

        Nebulin is a giant ($600{\sim}900$ kDa), modular sarcomeric protein proposed to regulate the assembly, and to specify the precise lengths of actin filamints in vertebrate skeletal muscles. Recently, There is an evidence that the nebulin also expressed in non muscle tissue, brain and liver. We identified a new isoform of nebulin from adult brain library by PCR screening. It contains two simple-repeats exon 165, 166 and linker-repeats exon $154{\sim}161$ except exon 159. The nebulin modules M160 to M170 (exon 150 to exon 161) has been shown to bind desmin. In mature striated muscle, desmin intermediate filaments surround Z-discs and link individual myofibrils laterally at their Z-discs and to other intracellular structures, including the costameres and the intercalated discs of the sarcolemma, sarcoplasmic reticulum, mitochondria, T-tubules, and nuclei. Therefore, it is an interesting possibility that the differential splice pathways within the linker region of nebulin modify the affinity of nebulin's interaction with desmin. The specific interactions of nebulin and desmin were confirmed in vivo by yeast two hybrid experiments. To verify in the cellular level the interaction between nebulin isoform and desmin, we transfected COS-7 cell with EGFP-tagged nebulin and DsRed-tagged desmin. Based on evidence showing that despite exon 159 was deleted, the new isoform of nebulin was interact with desmin. This suggest that nebulin in brain may interact with another intermediate filament. The conservation of these ligand-binding capacity in brain and skeletal nebulins suggest that nebulins may have conserved roles in brain and skeletal muscle.

      • SCISCIESCOPUS

        Enzyme fusion for whole-cell biotransformation of long-chain sec-alcohols into esters

        Jeon, Eun-Yeong,Baek, A-Hyong,Bornscheuer, Uwe T.,Park, Jin-Byung Springer-Verlag 2015 Applied microbiology and biotechnology Vol.99 No.15

        <P>Enzyme fusion was investigated as a strategy to improve productivity of a two-step whole-cell biocatalysis. The biotransformation of long-chain sec-alcohols into esters by an alcohol dehydrogenase (ADH) and Baeyer-Villiger monooxygenases (BVMOs) was used as the model reaction. The recombinant Escherichia coli, expressing the fusion enzymes between the ADH of Micrococcus luteus NCTC2665 and the BVMO of Pseudomonas putida KT2440 or Rhodococcus jostii RHA1, showed significantly greater bioconversion activity with long-chain sec-alcohols (e.g., 12-hydroxyoctadec-9-enoic acid (1a), 13-hydroxyoctadec-9-enoic acid (2a), 14-hydroxyicos-11-enoic acid (4a)) when compared to the recombinant E. coli expressing the ADH and BVMOs independently. For instance, activity of the recombinant E. coli expressing the ADH-Gly-BVMO, in which glycine-rich peptide was used as the linker, with 1a was increased up to 22 mu mol g dry cells(-1) min(-1). This value is over 40 % greater than the recombinant E. coli expressing the ADH and BVMO independently. The substantial improvement appeared to be driven by an increase in the functional expression of the BVMOs and/or an increase in mass transport efficiency by localizing two active sites in close proximity.</P>

      • SCISCIESCOPUS

        Intracellular transformation rates of fatty acids are influenced by expression of the fatty acid transporter FadL in <i>Escherichia coli</i> cell membrane

        Jeon, Eun-Yeong,Song, Ji-Won,Cha, Hee-Jeong,Lee, Sun-Mee,Lee, Jinwon,Park, Jin-Byung Elsevier 2018 Journal of biotechnology Vol.281 No.-

        <P>Fatty acids have a low permeability through the cell membrane. Therefore, the intracellular biotransformation of fatty acids can be slow due to supply limitations. The effects of expression level of the fatty acid transporter FadL in Escherichia coli on the biotransformations were investigated. The enhanced expression of FadL led to 5.5-fold increase of the maximum reaction rate V-max (i.e., 200 mu mol/min per g dry cells (200 U/g dry cells)) of the recombinant E. coli expressing a hydratase of Stenotrophomonas maltophilia in the periplasm with respect to hydration of oleic acid. The FadL expression level was also critical for oxidation of 12- and 10-hydroxyoctadecanoic acid by the recombinant E. coli expressing an alcohol dehydrogenase (ADH) of Micrococcus luteus. In addition, the multistep biotransformation of ricinoleic acid into the ester (i.e., (Z)-11-(heptanoyloxy) undec-9-enoic acid) by the recombinant E. coli expressing the ADH of M. luteus and a Baeyer-Villiger monooxygenase of Pseudomonas putida KT2440 was 2-fold increased to 40 U/g dry cells with expression of FadL to an appropriate level. The FadL expression level is one of the critical factors to determine whole-cell biotransformation rates of not only long chain fatty acids but also hydroxy fatty acids. This study may contribute to whole-cell biocatalyst engineering for biotransformation of hydrophobic substances.</P>

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