Complete genome sequence and comparative analysis of Streptomyces seoulensis, a pioneer strain of nickel superoxide dismutase

Jihoon Shin,Shinae Park,Jung‑Shin Lee,Eun‑Jin Lee,Hong‑Duk Youn 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background Streptomyces seoulensis has contributed to the discovery and initiation of extensive research into nickel superoxide dismutase (NiSOD), a unique type of superoxide dismutase found in actinomycetes. Still so far, there is no information about whole genome sequence of this strain. Objective To investigate complete genome sequence and perform bioinformatic analyses for genomic functions related with nickel-associated genes. Methods DNA was extracted using the Wizard Genomic DNA Purification Kit then sequenced using a Pacific Biosciences SMRT cell 8Pac V3 DNA Polymerase Binding Kit P6 with the PacBiov2 RSII platform. We assembled the PacBio longreads with the HGAP3 pipeline. Results We obtained complete genome sequence of S. seoulensis, which comprises a 6,339,363 bp linear chromosome. While analyzing the genome to annotate the genomic function, we discovered the nickel-associated genes. We observed that the sodN gene encoding for NiSOD is located adjacent to the sodX gene, which encodes for the nickel-type superoxide dismutase maturation protease. In addition, several nickel-associated genes and gene clusters-nickel-responsive regulator, nickel uptake transporter, nickel–iron [NiFe]-hydrogenase and other putative genes were also detected. Strain specific genes were discovered through a comparative analysis of S. coelicolor and S. griseus. Further bioinformatic analyses revealed that this strain encodes at least 22 putative biosynthetic gene clusters, thereby implying that S. seoulensis has the potential to produce novel bioactive compounds. Conclusion We annotated the genome and determined nickel-associated genes and gene clusters and discovered biosynthetic gene clusters for secondary metabolites implying that S. seoulensis produces novel types of bioactive compounds.

자료포락분석(DEA)를 이용한 보건의료의 효율성 측정

김남송,박홍섭,김은진,김설화,김정숙,김현아,김경숙,신지혜,최은정,양은진,유선미,유수진 圓光大學校 醫科學硏究所 2007 圓光醫科學 Vol.22 No.1

인쇄매체를 이용한 보건교육자료의 가시성에 영향을 주는 요인에 관한 연구

신준호,정은경,이정애,오승진,김신월 全南大醫大 2001 전남의대학술지 Vol.37 No.2

구강 내 디지털 인상채득을 통한 맞춤형 지대주와 시멘트 합착 후 나사형 임플란트 보철 수복 증례

홍용신,박은진,김선종,김명래,허성주,박지만 대한치과보철학회 2012 대한치과보철학회지 Vol.50 No.1

최근 디지털 인상채득 시스템이 개발되면서 이를 기존의 CAD/CAM 시스템과 접목한 인레이, 온레이, 크라운이나 간단한 계속가공의치 등의 고정성 보철물의 제작이 활발히 소개되고 있다. 그러나 임플란트 보철에서는 주로 기존의 아날로그식 인상채득 후 이를 스캔하여 제작하는 방법이 사용되어 왔으나, 구강 내 디지털 스캔한 데이터를 이용하여 나사형 보철물을 제작하는 술식은 아직 활발히 이루어지지 않았다. 이에 본 증례보고에서는 6명의 환자를 대상으로 구강 내 스캐너인 iTero를 사용하여 디지털 스캔을 시행한 후 그중 다섯명은 맞춤형 지대주와 시멘트 합착 후 나사형 임플란트 보철물(screw-retained implant prosthesis after cementation, or SCRP)을 제작하였고, 임상적으로 기능과 심미적인 측면으로 만족할 만한 결과를 얻었기에 이를 보고하고자 한다. Fixed dental prostheses such as inlay, onlay, crown, and bridge fabricated by CAD/CAM technique combined with digital impressions is getting popular due to the recent rapid progress of digital impression taking system. For the scope of implant prosthesis, however, digital intra-oral scan hasn’t been actively utilized for the fabrication of superstructures. In this case report, 6 cases of titanium-milled custom abutment based on the iTero intra-oral scan data were introduced, five of them were restored with screw-type prosthesis after cementation (SCRP) and the clinical results were satisfactory on restoring the function and esthetics.

LCA 기법을 적용할 제품의 우선순위 선정 방법론

신동희,권은선,문승식,김만영,정종연,허진호 한국전과정평가학회 2004 한국전과정평가학회지 Vol.5 No.1

본 연구의 목적은 일정한 논리적 절차를 거쳐서 객관적인 전과정평가의 우선순위를 선정하기 위한 일반적인 방법론의 개발이다. 우선 전과정평가 대상이 되는 제품(산업군)의 우선순위를 평가하기 위해서 제품의 환경성과 관련된 환경규제, 국내외 전과정평가 수행사례, 유해물질 등을 평가항목으로 설정하였다. 설정된 평가항목들에 대한 자료조사 및 분석을 통하여 세분화한 데이터베이스를 생성하였으며, 또한 학계 및 산업계의 의견을 반영하기 위해 전과정평가 전문가 및 산업계 관계자들을 대상으로 설문조사를 실시하였다. 설문조사결과를 바탕으로 각각의 평가항목 및 세부평가항목의 가중치를 부여하였고, 각 평가항목별로 점수를 설정하였다. 본 연구에서 개발한 방법론은 우선순위를 비교해야하는 제품(산업군)들이 각각의 평가항목에서 설정해놓은 해당 점수를 부여받게 된다. 부여받은 각 평가항목의 점수들을 모두 합산하면 평가대상이 되는 제품(산업군)에 대해 하나의 우선순위 평가 점수를 갖게 된다. 이와 같은 과정으로 방법론이 구축되었으며, 사례연구를 통하여 방법론의 타당성을 검증하였다. 이렇게 산출된 우선순위 평가점수를 평가하고자하는 제품(산업군)들과 비교하여 높은 점수를 갖는 제품(산업군)이 전과정평가를 먼저 실시하는 것이 바람직하다는 결론을 얻을 수 있다. The purpose of this study is to develop a general method to decide the priority of LCA application through a logical procedure. First of all, evaluation categories were developed to decide priority of subject products(industrial groups). The evaluation categories consist of environment regulations related to subject product, LCA case studies, hazardous substances, etc. The subdivided database was constructed through researching and analyzing information about the selected evaluation categories. In addition, a questionnaire was conducted to reflect the opinions from academic and industrial circles. Based on the result of the questionnaire, each evaluation category and sub-evaluation category were weighed differently, and certain points were assigned to them. According to the method developed in this study, comparing industrial groups or products for prioriting will have its total score that is a sum of points assigned to each evaluation category. This results that an industrial group or product having higher score than the others becomes the subject to apply LCA.

전자 서베잉을 이용한 서베이드 금관과 국소의치 프레임워크 디자인

홍용신,박은진,김성균,곽재영,허성주,박지만 大韓齒科保存學會 2011 Restorative Dentistry & Endodontics Vol.36 No.6

구강 스캐너와 CAD/CAM 기술로 인해 컴퓨터로 제작하는 방식이 활발히 보급된 고정식 보철 증례와는 달리, 의치를 디지털 방식으로 제작하는 데에는 아직 한계가 많다. 촉각을 느낄 수 있는 입력 장비와 전자 서베잉의 개념, 그리고 쾌속 조형법의 적용으로 인해 국소의치를 디지털로 제작할 수 있는 솔루션이 최근 소개되고 있다. 본 증례보고에서는 이러한 국소의치의 디지털 솔루션을 이용하여 총 다섯 명의 환자에서 서베이드 금관 및 국소의치를 제작하였다. 인상 과정상의 문제로 모형과 실제 치아 사이의 형태적 차이가 있었던 경우를 제외하고는 국소의치의 적합도는 우수했으며, 장착 후 안정성과 착탈 시의 유지력 또한 임상에서 적용하는 데에 문제가 없었다. There are still many limitations on fabricating dentures using digital method while computerized production of fixed prostheses utilizing intraoral scanner and CAD/CAM technology has propagated rapidly. Recently the digital solution of fabricating removable partial denture by applying haptic input device, electronic surveying, and rapid prototyping was introduced. In this case presentation, five patients were treated with surveyed crown and removable partial dentures by this digital solution. Fit of dentures was excellent except for one case which showed morphological difference between the actual teeth and that of master cast by the erroneous impression process. There also was not any problem of stability and retention after adaptation in the clinical setting.

Conceptual Study for Tissue-Regenerative Biodegradable Magnesium Implant Integrated with Nitric Oxide-Releasing Nanofi bers

Jin‑Kyung Jeon,Hyunseon Seo,Jimin Park,Soo Ji Son,Yeong Rim Kim,Eun Shil Kim,Jong Woong Park,Woong‑Gyo Jung,Hojeong Jeon,Yu‑Chan Kim,Hyun‑Kwang Seok,Jae Ho Shin,Myoung‑Ryul Ok 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.4

The excessive initial corrosion rate of Mg is a critical limitation in the clinical application of biodegradable Mg implantsbecause the device loses its fi xation strength before the fractured bone heals. This study suggests a new approach to overcomethis hurdle by accelerating tissue regeneration instead of delaying the implant biodegradation. As angiogenesis is anessential process in early bone regeneration, a Mg implant coated with electrospun nanofi bers containing nitric oxide (NO),which physiologically promotes angiogenesis, is designed. The integrated device enables adjustable amounts of NO to bestored on the NO donor-conjugated nanofi ber coating, stably delivered, and released to the fractured bone tissue near theimplanted sites. An in vitro corrosion test reveals no adverse eff ect of the released NO on the corrosion behavior of the Mgimplant. Simultaneously, the optimal concentration level of NO released from the implant signifi cantly enhances tube networkformation of human umbilical vein endothelial cells without any cytotoxicity problem. This indicates that angiogenesis canbe accelerated by combining NO-releasing nanofi bers with a Mg implant. With its proven feasibility, the proposed approachcould be a novel solution for the initial stability problem of biodegradable Mg implants, leading to successful bone fi xation.

음성 인식 헬스케어 시스템

신진우,조우승,조성빈,김재훈,정지은,사예지,백경동,박동규 순천향대학교 부설 산업기술연구소 2021 순천향 산업기술연구소논문집 Vol.27 No.2

As modern people's interest in health grows, wearable healthcare-related technologies that can collect biometric information and receive health-related services regardless of time and place are developing. This paper proposes the healthcare system based on voice recognition function. Unlike conventional wearable healthcare systems, the proposed system provides real-time telemedicine and diagnosis and the direct connection with doctors is possible through it. And almost all functions of the application in it are operated with voice commands to lower user access barriers. Because it deals with personal information including medical information, encryption algorithms have been applied between all data movements. Finally, the healthcare system proposed in this paper is expected to increase convenience in health care and reduce the medical gap.

중환자 전문간호사 수요추계연구

신성례,손은진 성인간호학회 2003 성인간호학회지 Vol.15 No.2

This descriptive study was conducted to project the number of critical care APNs needs in critical care units in an acute care hospital setting, up to the year 2020. Method: Necessary data and information were collected from various funded reports, professional literature, web-sites and personal visits to national and private institutions. The demand of critical care APNs were projected based on two critical care APNs per critical care units. Results: The projected number of critical APNs for the critical care units in acute care hospital settings as follows: 1) The total projected number f critical care APNs needed for critical care units were 1,270 in 2001. 2) By the year 2020, total number of projected needed in critical care units will be 1,080-1,700. Conclusion: In order to match the supply to the need, the professional organization should direct their efforts toward enacting legislation. Educational systems should identify strategies in initiation of critical care APNs programs in masters level as well as standardizing curriculums across the programs.

중추성 요붕증이 동반된 랑게르한스 세포 조직구증 1예

김진호,문준성,문선중,이지은,최재원,은미정,천경아,조인호,윤지성,원규장,이경희 신덕섭,이형우 영남대학교 의과대학 2005 Yeungnam University Journal of Medicine Vol.22 No.2

Central diabetes insipidus (DI) is a syndrome characterized by thirst, polydipsia and polyuria. Langerhans cell histiocytosis is one of the etiologies of DI. Recently we experienced a central DI associated with Langerhans cell histiocytosis. The 44 years old female patient complained right hip pain polydipsia and polyuria. We carried out water deprivation test. After vasopressin injection, urine osmotic pressure was increased form 109mOsmol/Kg to 327mOsmol/Kg (300%). Brain MRI showed a thickened pituitary stalk and at hot bone CT.CT guided biopsy revealed abnormal histiocytes proliferation and abundant lymphocytes, The final diagnosis was central DI associated with systemic Langerhans cell histiocytosis invading hip bone, L-spine and pituitary stalk. Desmopressin and etoposide chemotherapy were performed to the patient.