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Rifampicin에 의한 것으로 추정되는 위막성 대장염1예
김수현,이은우,정종혁,문승현,김동한,양혁승,오영상,김호동,김도현,박혁,박정환,박경옥,이영직 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2
Pseudomembranous colitis, caused by altering the normal colonic flora and allowing the multiplication of Clostridium difficile, is an deleterious adverse effect of antibiotics. But it is rarely reported by rifampicin. Rifampicin is one of the first line drug in the treatment of tuberculosis and many patients are exposed to its potential adverse effects. We experienced a patient that had abdominal discomfort and hematochezia due to pseudomembranous colitis after receiving antituberculous medication, and which was probably caused by rifampicin. A 82 years old man was admitted with abdominal discomfort and hematochezia for one week. On the past history he had been diagnosed as endobronchial tuberculosis about 4 months ago. Colonoscopy revealed multiple discrete whitish mucosal lesion on rectosigmoid colon, and histologic findings were consistent with pseudomembranous colitis. The antituberculous agents were discontinued and vancomycin was administered. The patient's symptoms were resolved within several days. There was no recurrence after reinstitution of the antituberculous agents excluding rifampicin. We report here on a case of pseudomembranous colitis probably due to rifampicin.
Metagenomic Analysis of Fungal Communities Inhabiting the Fairy Ring Zone of Tricholoma matsutake
( Miae Kim1 ),( Hyeok Jun Yoon ),( Young Hyun You ),( Ye Eun Kim ),( Ju Ri Woo ),( Yeong Gyo Seo ),( Gyeong Min Lee ),( Young Ja Kim ),( Won Sik Kong ),( Jong Guk Kim ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.10
Tricholoma matsutake, an ectomycorrhiza that has mutual relationships with the rootlet of Pinus denisflora, forms a fruiting body that serves as a valuable food in Asia. However, the artificial culture of this fungus has not been successful. Soil fungi, including T. matsutake, coexist with many other microorganisms and plants; therefore, complex microbial communities have an influence on the fruiting body formation of T. matsutake. Here, we report on the structures of fungal communities associated with the fairy ring of T. matsutake through the pyrosequencing method. Soil samples were collected inside the fairy ring zone, in the fairy ring zone, and outside the fairy ring zone. A total of 37,125 sequencing reads were obtained and 728 to 1,962 operational taxonomic units (OTUs) were observed in the sampling zones. The fairy ring zone had the lowest OTUs and the lowest fungal diversity of all sampling zones. The number of OTUs and fungal taxa inside and outside the fairy ring zone was, respectively, about 2 times and 1.5 times higher than the fairy ring. Taxonomic analysis showed that each sampling zone has different fungal communities. In particular, out of 209 genera total, 6 genera in the fairy ring zone, such as Hemimycena, were uniquely present and 31 genera, such as Mycena, Boletopsis, and Repetophragma, were specifically absent. The results of metagenomic analysis based on the pyrosequencing indicate a decrease of fungal communities in the fairy ring zone and changes of fungal communities depending on the fairy ring growth of T. matsutake.
결정형 규산분진에 폭로된 섬유모세포의 자가증식 : Evaluation by H₂O₂and PDGF-AA 와 TGFβ
안병용,김경아,문제혁,정진숙,김은경,임영 대한산업의학회 2000 대한직업환경의학회지 Vol.12 No.2
Objectives : The aim of this study is to find out the activity of autoproliferation of rat fibroblast exposed to crystalline silica and the role of mediators secreted from rat fibroblast. Methods : The effect of α-quartz on production of growth factor (platelet-derived growth factor-AA and transforming growth factorβ) from rat fibroblasts were evaluated by ELISA and immunocytochemical analysis. Gene expression of these growth factors in rat fibrobast exposed to crystalline silica was evaluated by RT-PCR. Furthermore, fibroblast proliferation by culture supernatant of rat fibroblast was assayed by the neutral red test. Results : The amounts of H2O2 and growth factors synthesized in rat fibroblasts were significantly increased by the stimulation of crystalline silica (α-quartz) , which showed the dose-dependent manner to the concentration of α-quartz with the maximum response at the dosage of 100 ㎍/㎠. The result of RT-PCR demonstrated that α-quartz induced gene expression of PDGF-AA and TGFβ in rat fibroblast. We also found that supernatant of α-quartz-cocultured rat fibroblast induced a significant proliferation of fibroblast. Conclusion : Crystalline silica directly induce functional change In fibroblast such as increased release of reactive oxygen species and growth factors. The products of these functional change promote fibroblast proliferation via autocrine loop.
오영상,이은우,정종혁,문승원,김수현,김동한,양혁승,박정환,박경옥,강성수,이영직 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2
Tracheobronchopathia osteoplastica is a rare disorder of unknown cause characterized by the projection of numerous bony or cartilaginous submucosal nodules into the tracheobronchial lumen with sparing of the posterior membranous portion of tracheobronchial tree. In the past, most of the cases were diagnosed as secondary finding at autopsy. Nowaday, the key component of the evaluation is flexible fiberoptic laryngoscopy and bronchoscopy, which typically demonstrates irregular spicules of subrnucosal bone and cartilage projecting into the tracheobronchial lumen and causing various degrees of airway obstruction. A 45 years old man was visited Sunchon St. Carollo hospital due to discomfort on throat. We saw several nodules just below vocal cord via laryngoscope. The computed tomography revealed multiple small calcified nqdules on trachea and both main bronchi. Pathologic finding of bronchoscopic biopsy showed abnormal proliferation of bony and cartilagious nodules in the tracheal submucosa. These fingdings were consistent with tracheobronchopathia osteoplastica. We report here on a case of tracheobronchopathia osteoplastica with review of literature.
F-118 Establish the database for antibiotic resistance profile of TB isolates in Korea
( Dong Hyeok Kim ),( Seung-eun Song ),( Se-mi Jeon ),( Gil-soo Lee ),( Sung-kyeong Lee ),( Seong-han Kim ),( Jae-il Yoo ),( Dong Hyeok Kim ),( Jae-ok Kim ) 대한결핵 및 호흡기학회 2017 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.124 No.-
Rapid, accurate diagnosis of drug resistant-TB is critical for timely initiation of treatment and control of the disease. Since WHO endorsed commercial rapid drug susceptibility test (DST) to identify mutations conferring resistance to the anti-mycobacterial drugs, several discrepant cases between rapid and conventional DST results were reported. Therefore, analysis of the prevalence of mutations associated with drug-resistance is important to understanding the current status of DR-TB as well as treatment. In this study, we established the MIC distribution with 476 clinical isolates from drug resistant risk group, namely retreatment TB patients, and MDR patients in South Korea. And we determined the resistance pattern of the anti-mycobacterial drugs and frequencies of resistance-associated mutations by sequencing analysis with rpoB genes for RIF, katG, inhA, and ahpC genes for INH, gyrA and gyrB genes for moxifloxacin, and rrs for kanamycin. Our results showed that 96% and 89.9% of RIF and INH resistance was associated with rpoB and katG (89.9%), inhA (31%), and/or ahpC (4.4%) gene mutation. And 95.1% of MOX resistance was related with gyrA (63.4%) and gyrB (6.4%) gene mutation and 46% KAN resistance were associated with rrs gene. Among the antibiotics resistance related gene mutations, Ser-531 of rpoB (60.8%), Ser315Thr of KatG (68%), -15 promoter region of inhA (92.1%), and point mutation of 21Glu, 94Asp and 95Ser at gyrA gene were the predominant mutation sites. Our database would help the interpretation of DST results and developing new diagnostic test.
Jun Hyeob Kim(Jun Hyeob Kim),Tae Hyeok Kim(Tae Hyeok Kim),Kyung Eun Lee(Kyung Eun Lee) 대한약학회 2022 약학회지 Vol.66 No.4
Bisphosphonate-related osteonecrosis of the jaw (BRONJ) is a severe side effect of bisphosphonate, which is usually prescribed as a treatment for diseases such as multiple myeloma, bone metastases, osteoporosis, and Paget disease. To investigate the risk factors of BRONJ, we conducted a nested case-control study using the Korean National Insurance Health Service-senior cohort. Patients newly diagnosed with BRONJ were selected as the case group, while the control group included patients who were never diagnosed with BRONJ. A risk-set sampling method was used to select the control group using age, sex, the date of the first prescription of bisphosphonate, and follow-up duration. Of the 22,236 subjects, 4,455 were included in the case group. Arthrosis, rheumatoid arthritis, neuropathies, myocardial infarction, renal disease, cancer, liver disease, and tooth extraction were associated with a higher incidence of BRONJ than subjects without such conditions (aOR, 95% CI: 1.58 [1.47-1.71], 1.46 [1.30-1.63], 1.31 [1.20-1.42], 1.23 [1.01-1.49], 1.36 [1.10-1.68], 1.27 [1.12-1.45], 1.63 [1.10-2.43], and 1.64 [1.53-1.76] respectively). In conclusion, of the factors examined, we confirmed that liver disease and tooth extraction were related to an increased risk of BRONJ.
Eun Jin Park,Joon Woo Song,Hyun Jung Kim,Chang-Soo Kim,Yeong Jun Song,Dae Hyeok Yang,Hongki Yoo,Jin Won Kim,Kyeongsoon Park 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.92 No.-
In this study, we report the in vivo imaging of reactive oxygen species (ROS)-overproducing pro-inflammatory macrophages in atherosclerotic plaques using afluorescent ROS nanosensor. We designedthe nanosensor by chemically conjugating hyaluronic acid (HA, a targeting ligand for CD44 receptor),chlorin e6 [Ce6; a near-infraredfluorescent (NIRF) dye], and a thioketal (TK) linker (ROS-degradablelinker). The self-assembled nanosensor emitted weak NIRF signals in normal physiological conditions,whereas it emitted strong NIRF signals under ROS-abundant conditions. The cytocompatible nanosensorshowed higher intracellular internalization via receptor-mediated endocytosis, which enabled thevisualization of intracellular ROS in pro-inflammatory macrophages. Moreover, we demonstrated thatthe nanosensor enabled the successful targeting and imaging of CD44- and ROS-overproducing pro-inflammatory macrophages in atherosclerotic plaques, as validated by confocal microscopy andimmunohistological analyses. These data suggest that our ROS nanosensor is suitable for ROS imaging inpro-inflammatory macrophages in vitro and in vivo and will be a promising approach for imagingatherosclerotic tissues and evaluating the effects of antioxidants.
Generation of a High-Growth Influenza Vaccine Strain in MDCK Cells for Vaccine Preparedness
( Eun-ha Kim ),( Hyeok-il Kwon ),( Su-jin Park ),( Young-il Kim ),( Young-jae Si ),( In-won Lee ),( Se Mi Kim ),( Soo-in Kim ),( Dong-ho Ahn ),( Young-ki Choi ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.6
As shown during the 2009 pandemic H1N1 (A(H1N1)pdm09) outbreak, egg-based influenza vaccine production technology is insufficient to meet global demands during an influenza pandemic. Therefore, there is a need to adapt cell culture-derived vaccine technology using suspended cell lines for more rapid and larger-scale vaccine production. In this study, we attempted to generate a high-growth influenza vaccine strain in MDCK cells using an A/Puerto/8/1934 (H1N1) vaccine seed strain. Following 48 serial passages with four rounds of virus plaque purification in MDCK cells, we were able to select several MDCK-adapted plaques that could grow over 10<sup>8</sup> PFU/ml. Genetic characterization revealed that these viruses mainly had amino acid substitutions in internal genes and exhibited higher polymerase activities. By using a series of Rg viruses, we demonstrated the essential residues of each gene and identified a set of high-growth strains in MDCK cells (PB1<sub>D153N</sub>, M1<sub>A137T</sub>, and NS1<sub>N176S</sub>). In addition, we confirmed that in the context of the high-growth A/PR/8/34 backbone, A/California/7/2009 (H1N1), A/Perth/16/2009 (H3N2), and A/environment/Korea/deltaW150/2006 (H5N1) also showed significantly enhanced growth properties (more than 10<sup>7</sup> PFU/ml) in both attached- and suspended-MDCK cells compared with each representative virus and the original PR8 vaccine strain. Taken together, this study demonstrates the feasibility of a cell culture-derived approach to produce seed viruses for influenza vaccines that are cheap and can be grown promptly and vigorously as a substitute for egg-based vaccines. Thus, our results suggest that MDCK cell-based vaccine production is a feasible option for producing large-scale vaccines in case of pandemic outbreaks.