Fundamental and conventional computer simulation for the stability of non-uniform systems

Wang, Chunping,Chen, Keming Techno-Press 2022 Advances in nano research Vol.13 No.2

The accurate assessment of the performance of nonuniform systems requires a thorough understanding of stability analysis. As a result, the theoretical modeling of the influence of various variables on the performance of small-scale nonuniform structures with conventional and non-conventional geometries is presented in this paper. According to the fundamental computer simulation based on mathematical and mechanical principles, the stability of the nonuniform structures is examined. Thus, a numerical procedure is used to simulate the stability and instability characteristics of the nonuniform small-scale structures via computer aid. Theoretic simulation methods provide a great deal of the design and production of small-scale structures at a low cost compared to experimental simulations. Thus, this paper provides a good presentation of the stability analysis of the nonuniform nanoscale structures with high accuracy without actual experimental.

Treatment of bladder cancer by geoinspired synthetic chrysotile nanocarrier-delivered circPRMT5 siRNA

Chunping Yu,Yi Zhang,Ning Wang,Wensu Wei,Ke Cao,Qun Zhang,Peiying Ma,Dan Xie,Pei Wu,Biao Liu,Jiahao Liu,Wei Xiang,Xing Hu,Xuewen Liu,Jianfei Xie,Jin Tang,Zhi Long,Long Wang,Hongliang Zeng,Jianye Liu 한국생체재료학회 2022 생체재료학회지 Vol.26 No.1

Background: Circular RNAs (circRNAs) have important functions in many fields of cancer biology. In particular, we previously reported that the oncogenic circRNA, circPRMT5, has a major role in bladder cancer progression. Therapy based on circRNAs have good prospects as anticancer strategies. While anti-circRNAs are emerging as therapeutics, the specific in vivo delivery of anti-circRNAs into cancer cells has not been reported and remains challenging. Methods: Synthesized chrysotile nanotubes (SCNTs) with a relatively uniform length (~ 200 nm) have been designed to deliver an siRNA against the oncogenic circPRMT5 (si-circPRMT5) inhibit circPRMT5. In addition, the antitumor effects and safety evaluation of SCNTs/si-circPRMT5 was assessed with a series of in vitro and in vivo assays. Results: The results showed that SCNTs/si-circPRMT5 nanomaterials prolong si-circPRMT5’s half-life in circulation, enhance its specific uptake by tumor cells, and maximize the silencing efficiency of circPRMT5. In vitro, SCNTs encapsulating si-circPRMT5 could inhibit bladder cancer cell growth and progression. In vivo, SCNTs/si-circPRMT5 inhibited growth and metastasis in three bladder tumor models (a subcutaneous model, a tail vein injection lung metastatic model, and an in situ model) without obvious toxicities. Mechanistic study showed that SCNTs/sicircPRMT5 regulated the miR-30c/SNAIL1/E-adherin axis, inhibiting bladder cancer growth and progression. Conclusion: The results highlight the potential therapeutic utility of SCNTs/si-circPRMT5 to deliver si-circPRMT5 to treat bladder cancer.

Effects of Nitrogen Concentration on Root Respiration Rate and Nonstructural Carbohydrates of Walnut Seedlings

Chunping Li,Shiwei Wang,Cuifang Zhang,Junjie Ding,Bin Ma,Rabigul Yasin,Zezheng Xue 한국원예학회 2020 원예과학기술지 Vol.38 No.3

Nitrogen is a critical determinant of forest growth and productivity, and root respiration rate and nonstructural carbohydrates are important parameters for evaluating the effects of supplied nutrients. Here, we assessed the effects of nitrogen on walnut seedlings (Juglans regia Linn.) that grew consistently for a year under favorable conditions. Seedlings were potted in seven concentrations of N availability, 0, 1, 2, 4, 8, 12, and 16 mmol·L1, and the specific root respiration rate and nonstructural carbohydrate content of the root system were determined via the offbody root system and the ketone color ratio methods, respectively. Nitrogen concentration was positively correlated with specific root respiration, and root diameter was the main factor related to specific root respiration and nonstructural carbohydrate content of walnut seedlings. Under the same nitrogen concentrations, the soluble total sugar and starch content of roots increased with increasing root diameter, while the specific root respiration increased with decreasing root diameter. Compared to the 0 mmol·L1 nitrogen concentration, the content of nonstructural carbohydrates in the root system and the specific root respiration were higher when nitrogen concentration was N16. Our comprehensive analysis showed that a nitrogen concentration of N16 improved the respiration rate of the seedling root system, significantly increased the content of nonstructural carbohydrates in the seedling root system, and effectively promoted the growth and development of the seedling root system of walnut seedlings.

An Automatic Method of Stereoscopic Video Synthesis from Monoscopic Video

Baoliang Wang,Chunping Hou,Xinyu Li,Can Ding 한국산학기술학회 2014 SmartCR Vol.4 No.4

This paper presents a novel depth-image-based rendering system for the generation of stereoscopic video from a monoscopic video sequence. The key point of the proposed system is that all the conversion procedures are accomplished automatically. First, we use Global Motion Estimation and an Adaptive Gaussian Mixture Model to segment the foreground and background. Second, different depth values are assigned to the foreground and background. Finally, image warping according to the depth map and stereoscopic video is synthesized. Experimental results show that the proposed method can effectively segment the foreground from the background and synthesize a satisfactory 3D effect, so the proposed approach is successful.

Nonenzymatic Electrochemical Immunosensor Using Ferroferric Oxide–Manganese Dioxide–Reduced Graphene Oxide Nanocomposite as Label for α-Fetoprotein Detection

Wensi Jian,Chunping Wang,Zuanguang Chen,Yanyan Yu,Duanping Sun,Liping Han,Lijuan Shi 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.10

A novel nonenzymatic electrochemical immunosensor was fabricated for quantitative detection of α-fetoprotein (AFP). The immunosensor was constructed by modifying gold electrode with electrochemical reduction of graphene oxide-carboxyl multi-walled carbon nanotube composites (ERGO–CMWCNTs) and electrodeposition of gold nanoparticles (AuNPs) for effective immobilization of primary antibody (Ab1). Ferroferric oxide–manganese dioxide–reduced graphene oxide nanocomposites (Fe3O4@MnO2–rGO) were designed as labels for signal amplification. On one hand, the excellent electroconductivity and outstanding electron transfer capability of ERGO–CMWCNTs/AuNPs improved the sensitivity of the immunosensor. On the other hand, introduction of rGO could not only increase the specific surface area for immobilization of secondary antibody (Ab2) but also build a synergetic effect to reinforce the electrocatalytic properties of catalysts. Fe3O4@MnO2–rGO nanocomposites were characterized by scanning electron microscope, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. Using AFP as a model analyte, the proposed sandwich-type electrochemical immunosensor exhibited a wide linear range of 0.01–50 ng · mL-1 with a low detection limit of 5.8 pg · mL-1. Moreover, the Fe3O4@MnO2–rGO-based peroxidase mimetic system displayed an excellent analytical performance with low cost, satisfactory reproducibility and high selectivity, which could be further extended for detecting other disease-related biomarkers.

Transcriptome profiling identifies immune response genes against porcine reproductive and respiratory syndrome virus and Haemophilus parasuis co-infection in the lungs of piglets

Jing Zhang,Jing Wang,Xiong Zhang,Chunping Zhao,Sixuan Zhou,Chunlin Du,Ya Tan,Yu Zhang,Kaizhi Shi 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.1

Background: Co-infections of the porcine reproductive and respiratory syndrome virus (PRRSV) and the Haemophilus parasuis (HPS) are severe in Chinese pigs, but the immune response genes against co-infected with 2 pathogens in the lungs have not been reported. Objectives: To understand the effect of PRRSV and/or HPS infection on the genes expression associated with lung immune function. Methods: The expression of the immune-related genes was analyzed using RNA-sequencing and bioinformatics. Differentially expressed genes (DEGs) were detected and identified by quantitative real-time polymerase chain reaction (qRT-PCR), immunohistochemistry (IHC) and western blotting assays. Results: All experimental pigs showed clinical symptoms and lung lesions. RNA-seq analysis showed that 922 DEGs in co-challenged pigs were more than in the HPS group (709 DEGs) and the PRRSV group (676 DEGs). Eleven DEGs validated by qRT-PCR were consistent with the RNA sequencing results. Eleven common Kyoto Encyclopedia of Genes and Genomes pathways related to infection and immune were found in single-infected and co-challenged pigs, including autophagy, cytokine-cytokine receptor interaction, and antigen processing and presentation, involving different DEGs. A model of immune response to infection with PRRSV and HPS was predicted among the DEGs in the co-challenged pigs. Dual oxidase 1 (DUOX1) and interleukin-21 (IL21) were detected by IHC and western blot and showed significant differences between the co-challenged pigs and the controls. Conclusions: These findings elucidated the transcriptome changes in the lungs after PRRSV and/or HPS infections, providing ideas for further study to inhibit ROS production and promote pulmonary fibrosis caused by co-challenging with PRRSV and HPS.

PREPARATION AND CHARACTERIZATION OF SILVER NANOPARTICLES/CARBON NANOFIBERS VIA ELECTROSPINNING WITH RESEARCH ON THEIR CATALYTIC PROPERTIES

Huan Liu,Jie Bai,Chunping Li,Shan Wang,Yarong Huang,QI WANG,WEIYAN SUN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.3

Ag nanoparticles (Ag NPs) embedded carbon nano¯bers (CNFs) were prepared by a new routewhich included chemical reaction process, electrospinning and calcination technique. The mor-phology and structure of the composite nano¯bers were investigated by scanning electron mi-croscopy, energy dispersive X-ray spectroscopy, transmission electron microscopy and X-raydi®raction. It indicated that Ag NPs were uniformly distributed in the CNFs. This e®ectivesynthesis method can be used to prepare other composite nano¯bers with functionality. The AgNPs/CNFs that served as supported catalysts were used in the styrene epoxidation by TBHP. The Ag NPs/CNFs catalyst showed its highly catalytic activity for the epoxidation of styrene(conversion: 40.6%, SO selectivity: 35.9%). This kind of composite nano¯ber membrane wasproved e®ectively catalytic activity and recycled easily in the styrene epoxidation.

Targeting treatment of bladder cancer using PTK7 aptamer-gemcitabine conjugate

Xiang Wei,Peng Yongbo,Zeng Hongliang,Yu Chunping,Zhang Qun,Liu Biao,Liu Jiahao,Hu Xing,Wei Wensu,Deng Minhua,Wang Ning,Liu Xuewen,Xie Jianfei,Hou Weibin,Tang Jin,Long Zhi,Wang Long,Liu Jianye 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Gemcitabine (GEM) is one of the first-line chemotherapies for bladder cancer (BC), but the GEMs cannot recognize cancer cells and have a low long-term response rate and high recurrence rate with side effects during the treatment of BC. Targeted transport of GEMs to mediate cytotoxicity to tumor and avoid the systemic side effects remains a challenge in the treatment of BC.Based on a firstly confirmed biomarker in BC-protein tyrosine kinase 7 (PTK7), which is overexpressed on the cell membrane surface in BC cells, a novel targeting system protein tyrosine kinase 7 aptamer-Gemcitabine conjugate (PTK7-GEMs) was designed and synthesized using a specific PTK7 aptamer and GEM through auto-synthesis method to deliver GEM against BC. In addition, the antitumor effects and safety evaluation of PTK7-GEMs was assessed with a series of in vitro and in vivo assays.PTK7-GEMs can specifically bind and enter to BC cells dependent on the expression levels of PTK7 and via the macropinocytosis pathway, which induced cytotoxicity after GEM cleavage from PTK7-GEMs respond to the intracellular phosphatase. Moreover, PTK7-GEMs showed stronger anti-tumor efficacy and excellent biosafety in three types of tumor xenograft mice models.These results demonstrated that PTK7-GEMs is a successful targeted aptamer-drug conjugates strategy (APDCs) to treat BC, which will provide new directions for the precision treatment of BC in the field of biomarker-oriented tumor targeted therapy.

Characteristics of the Infection of Tilletia laevis Kühn (syn. Tilletia foetida (Wallr.) Liro.) in Compatible Wheat

Zhaoyu Ren,Wei Zhang,Mengke Wang,Haifeng Gao,Huimin Shen,Chunping Wang,Taiguo Liu,Wanquan Chen,Li Gao 한국식물병리학회 2021 Plant Pathology Journal Vol.37 No.5

Tilletia laevis Kühn (syn. Tilletia foetida (Wallr.) Liro.) causes wheat common bunt, which is one of the most devastating plant diseases in the world. Common bunt can result in a reduction of 80% or even a total loss of wheat production. In this study, the characteristics of T. laevis infection in compatible wheat plants were defined based on the combination of scanning electron mi- croscopy, transmission electron microscopy and laser scanning confocal microscopy. We found T. laevis could lead to the abnormal growth of wheat tissues and cells, such as leakage of chloroplasts, deformities, disordered arrangements of mesophyll cells and also thickening of the cell wall of mesophyll cells in leaf tissue. What’s more, T. laevis teliospores were found in the roots, stems, flag leaves, and glumes of infected wheat plants instead of just in the ovaries, as previously reported. The abnormal characteristics caused by T. laevis may be used for early detection of this pathogen instead of molecular markers in addition to providing theoretical insights into T. laevis and wheat interactions for breed- ing of common bunt resistance.

Experimental Investigation on Permeability Evolution of Limestone Caprock under Coupled THM Processes

Qiangxing Zhang,Jianfeng Liu,Huining Xu,Yin Zeng,Chunping Wang,Lu Wang 대한토목학회 2019 KSCE Journal of Civil Engineering Vol.23 No.12

To investigate the gas sealing capacity of limestone as cap rock for gas reservoir at different burial depths, triaxial compression coupled with permeability tests of limestone subjected to different confining pressures and temperatures were carried out using the improved MTS 815 test machine. During the tests, acoustic emission (AE) monitoring system was also employed to estimate the rock damage. Test results demonstrated a degradation of triaxial compression strength with the increase in temperature and decrease of confining pressure. An empirical equation for the peak stress was established considering confining pressure and temperature. Three different permeability evolution patterns of limestone samples can be proposed based on the experimental results under different coupled thermo-hydro-mechanical (THM) conditions. According to the spatial-temporal characteristics of AE signals, permeability evolution was highly related to the fracture growth and connection in limestone. Ultimately, the experimental results revealed that the permeability of limestone gradually decreased with the increasing confining pressure and temperature. In addition, an exponential relationship between initial permeability and confining pressure was established.