Two new tirucallane triterpenoids from the leaves of Aquilaria sinensis

Jin Tang Cheng,Ya Qiong Han,Juan He,Xing De Wu,Liao Bin Dong,Li Yan Peng,Yan Li,Qin Shi Zhao 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.9

Two new tirucallane triterpenoids, aquilacallanesA–B (1–2), together with 15 known compounds(3–17) were isolated from the leaves of Aquilaria sinensis. The structures of these new compounds were elucidated onthe basis of extensive spectroscopic analyses. All compoundswere evaluated for their cytotoxic activity againstfive human cancer cell lines. The known compounds,ursolic acid (7) and 5,7,40-trimethoxyflavone (14), exhibitedweak cytotoxic activity against some cells.

Wide-Awake Primary Flexor Tendon Repair, Tenolysis, and Tendon Transfer

Jin Bo Tang 대한정형외과학회 2015 Clinics in Orthopedic Surgery Vol.7 No.3

Tendon surgery is unique because it should ensure tendon gliding after surgery. Tendon surgery now can be performed under local anesthesia without tourniquet, by injecting epinephrine mixed with lidocaine, to achieve vasoconstriction in the area of surgery. This method allows the tendon to move actively during surgery to test tendon function intraoperatively and to ensure the tendon is properly repaired before leaving the operating table. I applied this method to primary flexor tendon repair in zone 1 or 2, tenolysis, and tendon transfer, and found this approach makes tendon surgery easier and more reliable. This article describes the method that I have used for tendon surgery.

Haloactinobacterium album gen. nov., sp. nov., a halophilic actinobacterium, and proposal of Ruaniaceae fam. nov.

Tang, Shu-Kun,Zhi, Xiao-Yang,Wang, Yun,Wu, Jin-Yuan,Lee, Jae-Chan,Kim, Chang-Jin,Lou, Kai,Xu, Li-Hua,Li, Wen-Jun Microbiology Society 2010 International journal of systematic and evolutiona Vol.60 No.9

<P>A Gram-staining-positive, facultatively anaerobic, non-motile and moderately halophilic actinobacterium, designated YIM 93306<SUP>T</SUP>, was isolated from a salt lake in Xinjiang province, north-west China, and subjected to a polyphasic taxonomic study. Strain YIM 93306<SUP>T</SUP> grew in the presence of 2-16 % (w/v) NaCl and did not grow without NaCl. The peptidoglycan type was A4<I>α</I> with an l-Lys-l-Glu interpeptide bridge. The whole-cell sugars were glucosamine, arabinose, mannose and two unknown sugars. The predominant menaquinone was MK-8(H4). The major fatty acids were iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown phosphoglycolipid and one unknown phospholipid. The DNA G+C content was 68.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 93306<SUP>T</SUP> fell within the radius of the suborder <I>Micrococcineae</I>. Its closest phylogenetic neighbour was the type strain of <I>Ruania albidiflava</I> (AS 4.3142<SUP>T</SUP>; 96.2 % 16S rRNA gene sequence similarity), the sole recognized species of the genus <I>Ruania</I>. Sequence similarities between strain YIM 93306<SUP>T</SUP> and members of other genera of the suborder <I>Micrococcineae</I> were <95.2 %. On the basis of phylogenetic analysis, phenotypic characteristics and chemotaxonomic differences, a novel genus and species, <I>Haloactinobacterium album</I> gen. nov., sp. nov., is proposed. The type strain of the species is YIM 93306<SUP>T</SUP> (=DSM 21368<SUP>T</SUP> =KCTC 19413<SUP>T</SUP> =CCTCC AB 208069<SUP>T</SUP>). Based on phylogenetic characteristics and 16S rRNA gene signature nucleotide patterns, the genera <I>Ruania</I> and <I>Haloactinobacterium</I> gen. nov. are proposed to belong to a novel family, <I>Ruaniaceae</I> fam. nov.</P>

Five miRNAs as Novel Diagnostic Biomarker Candidates for Primary Nasopharyngeal Carcinoma

Tang, Jin-Feng,Yu, Zhong-Hua,Liu, Tie,Lin, Zi-Ying,Wang, Ya-Hong,Yang, La-Wei,He, Hui-Juan,Cao, Jun,Huang, Hai-Li,Liu, Gang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

MicroRNAs (miRNAs) play an essential role in the development and progression of nasopharyngeal carcinomas (NPC). Despite advances in the field of cancer molecular biology and biomarker discovery, the development of clinically validated biomarkers for primary NPC has remained elusive. In this study, we investigated the expression and clinical significance of miRNAs as novel primary NPC diagnostic biomarkers. We used an array containing 2, 500 miRNAs to identify 22 significant miRNAs, and these candidate miRNAs were validated using 67 fresh NPC and 25 normal control tissues via quantitative real-time PCR (qRT-PCR). Expression and correlation analyses were performed with various statistical approaches, in addition to logistic regression and receiver operating characteristic curve analyses to evaluate diagnostic efficacy. qRT-PCR revealed five differentially expressed miRNAs (miR-93-5p, miR-135b-5p, miR-205-5p and miR-183-5p) in NPC tissue samples relative to control samples (p<0.05), with miR-135b-5p and miR-205-5p being of significant diagnostic value (p<0.01). Moreover, comparison of NPC patient clinicopathologic data revealed a negative correlation between miR-93-5p and miR-183-5p expression levels and lymph node status (p<0.05). These findings display an altered expression of many miRNAs in NPC tissues, thus providing information pertinent to pathophysiological and diagnostic research. Ultimately, miR-135b-5p and miR-205-5p may be implicated as novel NPC candidate biomarkers, while miR-93-5p, miR-650 and miR-183-5p may find application as relevant clinical pathology and diagnostic candidate biomarkers.

Synthesis, Crystal Structures and Properties of Two Binuclear Supramolecular Complexes Based on Biphenyl-2,2'-dicarboxylic Acid Ligand

Tang, Jin-Niu,Pan, Gang-Hong,Li, Long,Tian, Wei-Man,Huang, Zhong-Jing Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.2

Two novel binuclear metal-organic coordination complexes [$Cd_2(Hdpa)_4(bpy)_2$] (1), [$Dy_2(dpa)_2(bpy)_2(NO_3)_2-(H_2O)_2$](bpy) (2) (where $H_2dpa$ = biphenyl-2,2'-dicarboxylic acid, bpy = 2,2'-bipyridine) have been synthesized under hydrothermal conditions and characterized by single crystal X-ray diffraction, spectral method (IR), elemental analysis (EA), powder X-ray diffraction (XRD), electronic spectra (UV-vis), fluorescent in the solid state and thermogravimetric analysis (TGA). Complexes 1-2 crystallizes isomorphously in the Triclinic space group P-1. The ${\pi}-{\pi}$ stacking interactions and hydrogen-bonds play a vital role in determining the crystal packing and construction of the extended 3-D supramolecular network.

Prognostic Significance of C-reactive Protein in Urological Cancers: a Systematic Review and Meta-analysis

Dai, Jin,Tang, Kun,Xiao, Wei,Yu, Gan,Zeng, Jin,Li, Wei,Zhang, Ya-Qun,Xu, Hua,Chen, Zhi-Qiang,Ye, Zhang-Qun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Background: C-reactive protein (CRP), considered as a prototypical inflammatory cytokine, has been proposed to be involved in tumor progression through inflammation. Recent studies have indicated CRP as a progostic predictor for urological cancers, but the results remain controversial. Materials and Methods: A systematic search of Medline, Scopus and the Cochrane Library was performed to identify eligible studies published between Jan 1, 2001 and Sep 1, 2013. Outcomes of interest were collected from studies comparing overall survival (OS), cancer-specific survival (CSS) and relapse-free survival (RFS) in patients with elevated CRP levels and those having lower levels. Studies were pooled, and combined hazard ratio (HR) of CRP with its 95% confidence interval (CI) for survival were used for the effect size estimate. Results: A total of 43 studies (7,490 patients) were included in this meta-analysis (25 for RCC, 10 for UC, and 8 for PC). Our pooled results showed that elevated serum CRP level was associated with poor OS (HR: 1.26, 95%CI: 1.22-1.30) and RFS (HR: 1.38 95%CI: 1.29-1.47), respectively. For CSS the pooled HR (HR: 1.33, 95%CI: 1.28-1.39) for higher CRP expression could strongly predict poorer survival in urological cancers. Simultaneously, elevated serum CRP was also significantly associated with poor prognosis in the subgroup analysis. Conclusions: Our pooled results demonstrate that a high serum level of CRP as an inflammation biomarker denotes a poor prognosis of patients with urological cancers. Further large prospective studies should be performed to confirm whether CRP, as a biomarker of inflammation, has a prognostic role in urological cancer progression.

Visible-light driven efficient elimination of organic hazardous and Cr (VI) over BiOCl modified by Chinese Baijiu distillers’ grain-based biochar

Xingyun Jin,Xiaolong Tang,Huan Li,Xiaoqian Tang,Jian zhang Li,Jun bo Zhong,Shulin Zhang,Dongge Ma 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.107 No.-

In this paper, distillers’ grains biochar (DGBC) was facilely gained by processing the distillers’ grains producedin Chinese Baijiu brewing, and the DGBC was used to modify BiOCl. The prepared DGBC/BiOCl compositephotocatalysts show elevated visible light-driven catalytic performance. X-ray diffraction (XRD)and scanning electron microscope (SEM) suggest the presence of DGBC affects the crystal growth andmicroscopic morphology of BiOCl. The low-temperature electron paramagnetic resonance (EPR) testaffirms more oxygen vacancies (OVs) exist in the DGBC/BiOCl composite photocatalysts. The 0.75-DGBC/BOC photocatalyst holds the highest efficiency for removal of rhodamine B (RhB), tetracycline(TC) and Cr (VI) compared with BiOCl, commercial TiO2 (P25) and other DGBC/BiOCl composites. Giventhe experimental results, the enhanced visible light responsive performance originates from the richOVs introduced by the modification of BiOCl with DGBC and the higher separation rate of photoexcitedcarriers.

叩兩端:錢基博文學史價値觀新探-以《現代中國文學史》爲中心

당김해 ( Jin Hai Tang ),육영용 고려대학교 중국학연구소 2008 中國學論叢 Vol.23 No.-

본문은 錢基博의 《現代中國文學史》 叩兩端의 새로운 고찰을 통하여 探其傳承, 察其所變, 觀其所執, 究其所因 네 가지 관점에서 錢基博과 그의 《現代中國文學史》의 문학사 가치관을 살펴보고자 한다. 1933年前後, 錢基博所著的《現代中國文學史》出版, 흔快引起文壇注意。至今的八十餘年間,對該著及其作者也時有硏究,評價文章也偶見於報刊。但筆者發現,對於該著何以王캐運起筆,以胡適結篇,以及作者蘊於其中的深意和由此透露的文學觀念等,多年來學界鮮有輪機,更乏深論。本論就試圖從這一角度契入,以《現代中國文學史》爲中心,以求深入探究大學者錢基博文學史價値觀的底蘊,度幾有助於當下文學史觀念的進一步探討。

Inhibition of Dll4/Notch1 pathway promotes angiogenesis of Masquelet’s induced membrane in rats

Qian Tang,Haimin Jin,Minji Tong,Gang Zheng,Zhongjie Xie,Shangkun Tang,Jialei Jin,Ping Shang,Huazi Xu,Liyan Shen,Yu Zhang,Haixiao Liu 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

The Masquelet’s induced membrane technique for repairing bone defects has been demonstrated to be a promising treatment strategy. Previous studies have shown that the vessel density of induced membrane is decreased in the late stage of membrane formation, which consequently disrupts the bone healing process. However, relatively little is known about certain mechanisms of vessel degeneration in the induced membrane tissue and whether promotion of angiogenesis in induced membranes can improve bone regeneration. Here, we showed that the Delta-like ligand 4/ Notch homolog 1 (Dll4/Notch1) pathway was relatively activated in the late stage of induced membrane, especially at the subcutaneous site. Then, DAPT, a classical γ-secretase inhibitor, was applied to specifically inhibit Notch1 activation, followed by up-regulation of vascular endothelial growth factor receptor 2 (VEGFR2) and CD31 expression. DAPTmodified induced membranes were further confirmed to contribute to bone regeneration after autogenous bone grafting. Finally, in vitro experiments revealed that knocking down Notch1 contributed to the functional improvement of endothelial progenitor cells (EPCs) and that DAPT-treated induced membrane tissue was more favorable for angiogenesis of EPCs compared with the vehicle group. In conclusion, the present findings demonstrate that Dll4/ Notch1 signaling is negatively associated with the vessel density of induced membrane. Pharmacological inhibition of Notch1 attenuated the vessel degeneration of induced membrane both in vitro and in vivo, which consequently improved bone formation at the bone defect site and graft resorption at the subcutaneous site.

Investigation on the interactions of scutellarin and scutellarein with bovine serum albumin using spectroscopic and molecular docking techniques

Hao Tang,Nian-Guang Li,Zhi-Hao Shi,Yu-Ping Tang,Qian-Ping Shi,Ze-Xi Dong,Peng-Xuan Zhang,Jin-ao Duan 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.10

The binding abilities of scutellarin (Scu) andscutellarein (Scue) with bovine serum albumin (BSA) wereinvestigated using equilibrium dialysis, high performanceliquid chromatography, fluorescence spectroscopy, competitivesite marker and molecular docking. The resultsshowed that the average protein binding ratios of Scu andScue with BSA were (79.85 ± 1.83) and (85.49 ± 1.21) %respectively. Under simulated physiological conditions, thefluorescence data indicated that Scu and Scue bound withBSA through a static mechanism. The thermodynamicparameters indicated that the interactions of Scu-BSA andScue-BSA mainly occurred by van der Waals forces andhydrogen bonds and it was easier for Scue to bind withBSA than Scu, indicating that the glucuronic acid moleculein Scu decreased the binding affinity. Site competitivemarker experiments showed that the binding sites of Scuand Scue mainly located within the sub-domain IIA ofBSA. Furthermore, molecular docking studies indicatedthat one BSA could bind three Scue, while one BSA couldcarry only two Scu. All these results clearly indicated theinteractions of Scu and Scue with BSA, which will lay thefoundation for further research to determine the pharmacologyand pharmacodynamics of Scu and Scue for treatingischemic cerebrovascular disease.