Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

저체중 또는 과체중 청소년에서 캠프 프로그램을 이용한 영양 교육효과에 관한 연구

성미경,승정자,류화춘,박재년,박동연,최미경,조경옥,최선혜,이윤신,김유경,이은주 대한지역사회영양학회 2003 대한지역사회영양학회지 Vol.8 No.4

This study was performed to evaluate the effectiveness of a nutrition education program developed for nutritionally imbalanced adolescents. A summer nutrition camp was held for 23 overweight and 16 underweight subjects. Its effectiveness with regard to was evaluated at the end of the camp and 6 months later. Nutrition knowledge, nutritional attitude, food behavior, nutrient intake, exercise habit and ideal body figures desired by the subjects. The results showed significantly higher nutrition knowledge scores at the end of the camp as compared to those obtained prior to the camp program, and these scores were maintained for at least six months. Nutrition attitude scores also improved after the education program, and these improved scores also lasted for 6 months. However, the food behavior scores measured 6 months after the education program were not significantly different from those obtained prior to the camp. Also, the exercise habit, the ideal body figures and the body figures desired by the subjects remained unchanged. When nutrient intakes of subjects were assessed before the program and 6 months later, the mean daily vitamin C intake was significantly increased after the education program. Also, the intake of iron from plant food sources increased in the overweight subjects, while less iron from animal source were consumed by the underweight subjects. Both groups tended to consume more vegetables and fruits 6 months after the education program which may have contribute to the higher vitamin C and plant-based iron intakes. These results indicate that a 4-day nutrition education camp program sustained changes in nutrition knowledge and nutrition attitude for 6 months. The increased intake of vegetables and fruits was also achieved through this education program. However, changes in dietary behavior in adolescents may require repeated education.

심근 허혈-재관류 백서 모델에서 mitogen-activated protein kinase 활성화에 대한 α-lipoic acid의 효과

김연경,박은미,최준호,윤경호,유남진,이은미,김남호,정진원,김민선,박병림,오석규 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

일부 성인의 치과 공포감 관련 요인

김수경 ( Soo Kyung Kim ),김미희 ( Mi Hee Kim ),최현지 ( Hyun Ji Choi ),황정근 ( Jung Geun Hwang ) 한국치위생학회(구 한국치위생교육학회) 2014 한국치위생학회지 Vol.14 No.6

Objectives: The purpose of the study was to investigate the related factors to dental fear in some adults. Methods: A self-reported questionnaire was filled out by 320 adults in Seoul and Gyeonggi-do from April to June, 2013. Except 14 incomplete answers, data were analyzed by SPSS 18.0 program. The questionnaire consisted of general characteristics of the subjects(7 questions), dental fear related factor(18 questions), distrust for dentist(15 questions), and distrust for dental hygienist(11 questions). The instrument for dental treatment fear was Dental fear Survey(DFS) adapted from Kleinknecht and partly modified by Choi. Out of 20 questions, 18 questions were reconstructed and score by Likert 5 scale. Higher score means higher dental fear. Cronbach alpha was 0.959 in the study. The distrust for dentist and dental hygienist was adapted from Choi and reconstructed and score by Likert 5 scale. Higher score means higher distrust for dentist and dental hygienist. Cronbach alpha was 0.937 in distrust for dentist and 0.874 in distrust for dental hygienist in the study. Results: Those who experienced dental pain tended to have dental fear. The reasons for dental fear were as follows; bad breath(2.96 out of 5 points, hereafter represented as of 2.96/5), dental caries(2.88/5), missing teeth(2.87/5) and tooth pain(2.77/5). The distrust for the dentist was the main dental fear in the adults. Conclusions: The dental fear was closely related to gender, experience of dental pain, oral symptoms and distrust for the dentist. As the psychological pain in the patients was mainly influenced by the trustful atmosphere, it is necessary to make the patient easy before treatment.

제조조건을 달리한 유과의 품질 비교

최영희,윤은경,강미영 동아시아식생활학회 2000 동아시아식생활학회지 Vol.10 No.1

Physicochemical and sensory characteristics of Yukwa(fried rice cookie) made by different processing condition were investigated to establish the optimum expansion of Yukwa. The expansion rate and crispness of Yukwa prepared by soaking waxy rice for 24hours was satisfactory. Addition of 15% soy slurry gave better effect on increasing the expantion rate, crispiness and sensory preference than no addition of soy slurry. Yeast, Soju(distilled liquor), Mackeali(rice wine, turbid) and Chunju(rice wine, clear) as expansion reagent were added to Yukwa preparation. Yeast and Mackeali among tested expansion reagent showed better expansion rate and lower hardness measured by texturometer than Chunju. Yukwa added Chungju showed more fine structure and lower expantion rate than others, Microwave puffed Yukwa was less expansion rate, but same level of crispiness and hardness compared with oil puffed. And no difference in sensory characteristics such as degree of crispiness and stickiness of microwave puffed and oil puffed.

대구지역 성인남여의 혈압, 혈당, 혈중지질 및 체지방분포에 관한 연구

최미자,김미경 啓明大學校 生活科學硏究所 1993 科學論集 Vol.19 No.-

Studied relating obesity and morbidity have primarily focused on relative body weight and total adipose mass. However, an accumulating number of both epidemiological and clinical studies have now shown an independent association between body fat distribution or anthropometric type and disease. Abdominal obesity, i.e., an increased ratio of abdomen to hip circumfernce, has been shown to be associated with an increased risk for glucose intolerance, coronary artery disease and stroke in both men and women. This study was an attempt to observe various factions, such as dietary intake, lipid profile, obesity level, and body fat distribution in healthy men and women in the Taegu, urban area. The following anthropometric measurements were made on all participants(44 men, 41 women): weight, height; the abdominal circumferences was measured at the narrowest part of the torso as seen from the anterior view or at the level of the natural waist line, and the hip circumference at the point of maximum protuberance of the buttocks. The waist-to-hip circumference ratio was used as a measure of abdominal obesity. The results were as follows; 1. Among the subjects 19% were obese and the mean BMI of men was 22.8 and of women was 23.8. 2. Using the waist-to-hip circumference ratio, women belonging to upper body obesity(WHR>0.84) were 49%, and men belonging to upper body obesity (WHR>0.90) were 36%. 3. The average of daily energy and nutrient intake did not distinguish between men and women. The average intake of nutrients and energy were above the RDA in men and women. Percentage of energy is that carbohydrate: protein: fat=68:13:19. 4. The mean total cholesterol concentration was 202㎎% and 200㎎% in men and women respectively. The mean triglyceride level was 74㎎/㎗ and 68㎎/㎗ in men and women respectively. 5. When subjects were divided into obese and nonobese groups according to the RBW or BMI, energy intake was not significantly different between the two groups, but the obese group had higher female ride concentration and blood pressure. 6. When subjects were divided into upper or lower body obesity groups by WHR, the group of female of upper body obesity subjects showed higher cholesterol levels and blood pressure than the lower body obesity subjects. On the other hand there was no difference between the two groups in men. 7. The blood glucose level correlated significantly with cholesterol and triglyceride levels. WHR correlated significantly with blood pressure and BMI. In conclusion, these findings suggest that upper body obese subjects will manifast an altered lipid metabolism.

Zinc protoporphyrin과 다양한 용매조건이 MTT formazan의 발색 안정성에 미치는 영향

박경아,최유미,최현아,김미리,김현정,홍정일 서울여자대학교 자연과학연구소 2011 자연과학연구논문집 Vol.23 No.-

An assay method using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltertrazolium bromide (MTT) is commonly utilized for analyzing cell viability. In this method, MTT tetrazolium is converted to formazan by mitochondrial reductase of viable cells. The formzan formed is, however, not stable; the stability is affected by various factors. In the present study, effects of zinc protoporphyrin (ZnPP) and different solvents on color response of formazan dye were investigated. MTT formazan was stable under dark; light enhanced the color degradation of formazan. ZnPP induced rapid degradation of formazan under light condition in DMSO solution. Decolorization of formazan was faster in aqueous solvents, including distilled water, HCl as well as Triton X-100 solution. Formazan dye was most unstable in HCl (0.1 N) regardless of light or presence of ZnPP. Color response of formazan dye in NaOH (0.1 N) was enhanced by ~2 folds as compared to one in DMSO. MTT formazan was stable in this condition; the color change was less than 10% even under light during 2 hr. ZnPP, however, induced rapid decolorizaion of formazan dissolved in NaOH under light. H2O2, an oxidizing agent, did not affect color response of MTT formazan. The present results suggest that various factors could affect the color response of MTT formazan, which needs to be considered carefully in MTT assay. Key words:MTT tetrazolium, formazan, zinc protoporphyrin, cell viability, sodium hydroxide.

형광 검출 흐름 주입 분석법에 의한 암모늄 이온의 선택적 정량

최용욱,김미경,최윤정 전주대학교 자연과학종합연구소 1997 전주대학교 자연과학연구소 학술논문집 Vol.10 No.3

Selective determination of aqueous ammonia by flow injection analysis with fluorometric detection was described. The length of the mixing coil and the reaction coil, the composition of organic solvent, the sulfite concentration, the pH of buffer solution and the reaction temperature were optimized as analytical parameters for selectively determining NH_4^+/NH_3 by using FIA setup. The limit of detection (LOD) and the repeatability for aqueous ammonia were 0.04 ㎍/L (3SD) and better than 1%, respectively. This analytical condition showed considerable selectivity for aqueous ammonia over amino acids studied. Examples of the use of this FIA system were given for the anlysis of aqueous ammonia in lab water, tap water and groundwater.

Clostridium sporogenes의 Protoplast화에 따른 微細構造의 變化

崔慶浩,金美林 대구효성가톨릭대학교 1988 연구논문집 Vol.37 No.1

Pencillin-sucrose에 依한 溶菌의 경우에는 原形質 분리현상이 일어나 후 중아부와 말단부에서 細胞壁이 溶解되어 안정한 Plotoplast가 얻여졌으나 lysozyme-sucrose에 依한 溶菌의 경우에는 細胞壁이 菌體 전반에 걸쳐 급격히 溶解되었으며 原形質膜 �m나 부분덕으로 손상되므로 protoplast의 효율면에서 penicillin-sucrose 溶菌系의 경우보다 열등하였다. formalin 處理菌은 原形質 분리 및 細胞壁 溶解 어느것도 확인되지 아니하였다. 이상의 結果로 부터 細菌의 protoplast化에는 autolysin이 중요한 기능을 tngodgku 細胞壁 溶解에 선행하여 原形質이 분리되는 것으로 判斷되었다. Growing cells of Clostridium sporogenes (IFO 12636) were rapidly lysed by exposouring them to the penicillin G or egg white lysozyme, and they were successfully converted into the protoplasts in the concomitant presence of 0.35 M sucrose. The intact cells were rod shaped and their cytoplasms were entirely surrounded by rigid cell walls. The wall formed a smooth layer and well attached to outside of the plasmamembrane. In the penicillin-sucrose lytic system, cell wall was begin to be lysed at the both ends or middle part of the cell. It was apparent that the beginning site of cell wall sysis was accorded to the site of plasmolysis. The sites of wall lysis were clearly observed by forming bulbs. The bulbs were cytoplasmic components surrounded by plasmamembrane, and they were extruded through the gap in the cell wall formed by lysis of wall. As thelysis progress, the bulbs became larger and cell wall grdually removed from the surface of the bulbs. Finally, the cells were converted into protoplasts which have no intact cell wall on their surface. The cells took for 120 minutes of cultivation for conversion from bacterial rods to spherical protoplasts. In the lysozyme-sucrose lytic system, lysis progresssed so rapidly over shole surface of the cell and it was hard to prevent the plasmamembrane from to be burst. Gowever, when formalin-treated cells were applied to lysozyme-sucrose lytic system, the cells showed little plasmolysis and morphological changes. From these results it is clear that plasmolysis makes enable the cells to be lysed rapidly, and cellular lytic enzyme, autolysin, do and important role in initiation of wall lysis even in the presence of supplemented lytic enzyme.

흐름 주입 분석법에 의한 하이포아염소산 이온의 분광학적 정량

崔鎔旭,李秀令,金美警 전주대학교 1999 論文集 Vol.24 No.2

흐름주입 분석법(FIA)에 의한 간접 요오드화 UV 검출법으로 하이포 차아염소산 이온을 신속하게 정량하는 방법을 연구하였다. FIA장치를 최적화하기 위해 용리액의 pH, 요오드화 이온의 농도, 혼합 및 반응코일의 길이, 시료주입량 및 유속, 온도 및 검출파장에 대한 변화를 고찰하였다. pH 8.3에서 하이포아염소산 이온은 요오드화 이온을 선택적으로 산화시키고 자신은 염화이온으로 환원되었다. 방해이온을 제거하기 위한 가리움제로서 에틸랜다이아민이 가장 좋은 효율을 나타내었다. 하이포아염소산 이온의 검정곡선은 0.03∼0.3mg/L의 농도범위에서 0.999이상의 직선성을 나타내었고, 검출한계는 0.007mg/L 이었다. 이러한 분석조건하에서 몇몇 전주근교 상수 중 총 잔류염소의 농도를 정량하였다. The determination of hypochlorite ion by flow injection analysis(FIA) with iodometric UV detection was investigated. The pH of the acid stream, the concentration of the iodide ion, the length of the mixing and reaction coils, the injection sample size, and flowrate were optimized as parameters for determining total chlorine residuals by FIA method. Iodide was selectively oxidized to iodine by hypochlorite ion at pH 8.3. Ethylenediamine as masking agent for masking interference ions from the sample was given the best efficency. Calibration curve presented linear range of 0.03-3mg/L for hypochlorite ion with a correlation coefficient of 0.999 or better, The detection limit was found to be 0.007mg/L for hypochlorite ion, Under these analytical conditions, total chlorine residuals in several tap water sampled in the city of Jeonju were analyzed.