한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

Effects of Phenytoin and Diazepam on the Seizure Activity in the Cortical Dysplasia Animal Models

Kim, Si-Hyung,Choi, In-Sun,Cho, Jin-Hwa,Park, Eun-Ju,Jang, Il-Sung,Choi, Byung-Ju,Kim, Hyun-Jung,Kim, Young-Jin,Nam, Soon-Hyeun The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Dysplasia-associated seizure disorders are markedly resistant to pharmacological intervention. Relatively little research has been conducted studying the effects of antiepileptic drugs(AEDs) on seizure activity in a rat model of dysplasia. We have used rats exposed to methylazoxymethanol acetate(MAM) in utero, an animal model featuring nodular heterotopia, to investigate the effects of AEDs in the dysplastic brain. Pilocarpine was used to induce acute seizure in MAM-exposed and age-matched vehicle-injucted control animals. Field potential recordings were used to monitor amplitude and numbers population spikes, and paired pulse inhibition in response to stimulation of commissural pathway. Two commonly used AEDs were tested: diazepam 5, 2.5mg/kg;phenytoin 40, 60mg/kg. Diazepam(DZP) and phenytoin(PHT) reduced the amplitude of population spike in control and MAM-exposed rats. However, the amplitude of population spike was nearly eliminated in control rats as compared to the MAM-exposed rats. Pharmaco-resistance was tested by measuring seizure latencies in awake rats after pilocarpine administration(320mg/kg, i.p.) with and without pretreatment with AEDs. Pre-treatment with PHT 60 mg prolonged seizure latency in control rats, but not in MAM-exposed animals. The main findings of this study are that acute seizures initiated in MAM-exposed rats are relatively resistant to standard AEDs assessed in vivo. These data suggest that animal model with cortical dysplasia can be used to screen the effects of potential AEDs.

(±)-Camphor가 ICR 마우스 수컷의 간 cytochrome P450 효소 활성에 미치는 영향

오은경,박형건,배기현,최옥진,최은경,최창근,한진희,정태천 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.4

Effects of (±)-camphor on liver cytochrome P450 enzymes were investigated in male ICR mice. Mice were treated intraperitoneally with 0, 200, 400 and 800 mg/kg of (±) -camphor in corn oil for 3 consecutive days. Twenty four hr after the final treatment, the animals were subjected to necropsy. The activities of serum aspartate aminotransferase and serum alanine aminotransferase were slightly changed by the treatment with (±)-camphor at the doses used. Administration o(±)-camphor to mice significantly induced the hepatic activities of pentoxyresorufin O-depentylase and benzyloxyresorufin O-debenzylase and weakly induced ethoxyresorufin O-deethylase in dose-dependent manners. The present results suggested that (±)-camphor might act as a relatively specific inducer of hepatic cytochrome P450 213 in male ICR mice.

다발성 간종괴의 형태로 나타난 특발성 과호산구 증후군 치험1예

최승오,연규민,이승민,김순제,왕준호,박형석,이재동,진춘조,이창훈,신현준,김정은 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Hypereosinophilic syndrome is characterized by persistent blood eosinophilia of 1,500/ul or more in the absence of known causes and multiorgan dysfunction by eosinophil-related tissue damage. In Korea, some cases of hypereosinophilic syndrome with hepatic involvement have been described with prolonged benign clinical courses. Most of them were diffuse or multifocal lesions in imaging studies, and a few case presenting as a single mass has been described. Here we report a case of hypereosinophilic syndrome with hepatic involvement in a 65-year-old man who presented with multifocal mass lesions. By abdominal US and CT scan, multiple, ill-defined nodules were detected in throughout the liver. Liver biopsy revealed severe eosinophilic hepatitis, suggestive of hypereosinophilic syndrome. Hepatic mass lesions improved after steroid administration for several weeks.

호텔 고객의 뷔페 메뉴품질 만족도에 관한 연구

최수근,이연정,이진형 한국식생활문화학회 2004 韓國食生活文化學會誌 Vol.19 No.5

This study aims at providing useful information to establish subdivided marketing direction by deriving factors of significant extent and practicable extent of buffet users toward menu quality among super deluxe hotels in Gyeongju, and furthermore aims at presenting implications for efficient management result and sales increase of buffet in the future. The result of positive analysis is as follows. Firstly, in result of analyzing difference between importance and performance of hotel buffet in Gyungju area, importance is much higher than performance. Secondly, respondents consider that the. most important menu of hotel buffet is main dish such as hot food and instant food rather than menu such as beverage, soup and salad. When it comes to attribute of menu quality, it appeared that respondents put highly significant extent on sanitariness of food, freshness of food, flavor of food, and seasonality and variety of menu. Thirdly, oriental instant food is included in the second quadrant of IPA regarding buffet menu which should be intensively managed. And three attributes such as temperature of food, time of changing food, and creativity of menu are included in the second quadrant of IPA graph by attribute of hotel buffet's menu quality. Lastly, in IPA graph by hotel, the following menus of each hotel need to be intensively managed: Oriental instant food of A hotel, Western cold food of B hotel, Oriental instant food, Western instant food, and Oriental dessert of C hotel, and Western porridge·soup, Oriental instant food, and Western instant food of E hotel. The aforementioned menus should be improved.

시판 감귤과즙음료의 품질특성에 관한연구

최진영,심재형,이택수 서울여자대학교 자연과학연구소 1991 자연과학연구논문집 Vol.2 No.-

시판 감귤과즙음료의 품질 특성을 규명할 목적으로 유기산, 유리당, 색도, 미생물 수 등을 측정한 결과는 다음과 같다. 1. 비타민 C는 13.77-41.91mg%로서 강화제로 비타민 C의 첨가에 의해 희석과즙음료와 천연과즙음료 간에는 큰 차이가 나타나지 않았다. 2. pH는 2.74-3.78로 나타났으나 희석과즙음료가 낮았고, 총산은 희석과즙음료가 0.43-0.45%, 과즙음료가 0.57-0.64%, 천연과즙음료가 현저히 높았다. 3. 유기산은 citric acid 0.26-0.80%, malic acid 0.014-0.39%, succinic acid 0.010-0.185%, tartaric acid 0-0.069%로 어느 과즙음료나 citric acid의 함량이 많은 비율을 차지하였다. 주 유기산인 구연산의 함량이 천연과즙 0.54-0.80%, 과즙음료 0.37-0.40%, 희석과즙음료 0.26-0.28%로서 천연과즙이 현저히 높았다. 4. 환원당은 5.41-11.83%, 총당은 7.65-13.76%로 나타났고, 이를 당은 천연과즙음료보다 희석과즙이나 과즙음료가 대체로 높은 경향을 보였다. 5. 유리당은 fructose 1.90-7.92%, glucose 1.86-4.74%, sucrose 0.45-3.35%로 희석과즙이나 과즙음료는 이성화당의 첨가로 fructose가 가장 많고 sucrose가 미량이었으나 천연과즙은 sucrose가 희석이나 과즙음료보다 함량이 높았다. 6. 아미노테질소는 천연과즙음료가 42.92-53.75mg%, 과즙음료가 11.36-18.09mg%, 희석과즙음료가 2.0-2.8mb%로 천연과즙이 높아 감칠맛이 좋은 것으로 고려된다. 7. 색도는 명도(L)와 황색을 나타내는 b값은 천연과즙음료가 높은 것으로 나타났다. 8. 무기성분은 K가 13.33-199.34mg%으로 가장 많이 함유되었고, 그 다음 Na, Ca, Mg 순으로 많았으나 시험구간에는 차이가 거의 없는 편이었다. 9. 미생물수는 효모는 과즙음료와 천연과즙음료의 일부에서, 내산성 세균은 일부의 희석과즙음료에서 검출되었으며, 총 세균수와 곰팡이는 거의 모든 시료에서 각각 나타났다. 그러나 대부분의 시험구에서 이들 미생물 수는 10/ml개 정도였다. Commercial orange juices were analyzed to elucidate the composition of organic acid, tree sugar, color and microorganism count. There was no sigfinicant difference of the content of vitamin C between the diluted orange juice to be added vitamin C and natural orange juice. The pH of all the tested samples was in the range of 2.74-3.78. Total acid of natural juice shows the highest level among all samples. Citric acid shows the highest level among the organic acids. Among the samples, the citric acid content of natural juice was showed the highest level at 0.54-0.80% rather than the that of 50% orange juice and 10% orange juice was 0.37-0.40% and 0.26-0.28%, respectively. The content of malic acid, succinic acid and tartaric acid was 0.014-0.39%, 0.01-0.19% and 0-0.069%, respectively. The content of reducing sugar was 5.41-11.83% and the content of total sugar was 7.65-13.76% for all samples. Those sugar content of natural juice were showed higher than those of 50% orange juice and 10% diluted juice. Among the free sugar, the content of fructose, glucose and sucrose was 1.90-7.92%, 1.86-4.74% and 0.45-3.35%, respectively. In the case fo the diluted orange juices, the content of fructose was high, but the content of sugar was extreemly low. It may be resulted from the extra addition of fructose syrup. The sucrose of natural juice was higher than that of the diluted juice. The amount of amino nitrogen for natural juice was 42.92-53.75mg%, and it shows higher level than that of amino nitrogen of 50% diluted and 10% diluted orange juice. The increased amount of amino nitrogen for natural juice seemed to provide good taste. Natural juice shows higher value of lightness(L) and yellow color(b) than diluted juice. The concentration of potassium was the highest level among the mineral content. The concentration of sodium, calcium and magnesium relatively high, but there was no difference among the tested groups. Yeast was detected at the natural juice and 50% diluted juice. Acid tolerant bacteria was only detected at the 10% diluted juice. Total aerobic bacteria and mold was also detected at the all samples. The microorganism count was around 10 per ml for all samples.

Lesch-Nyhan syndrome 환아의 oral self-mutilation 에 대한 증례보고

최병재,최형준,이제호,전진용 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.1

1. 하순에 심한 self-mutilation wound를 야기한 Lesch-Nyhan Syndrome 환아에서 상하악 유전치에 치관부 절단 및 치수 절세술을 시행하여 치아에 의한 자해를 방지하였다. 2. 장치를 이용한 치료가 어려운 경우 발치가 고려되기도 하나 본 증례에서는 발치보다 보존적인 술식으로 만족할 만한 결과를 얻었다. Self-injurous behavior is defined as deliberate harm to one's own body without suicidal intent. It usually occurs as head banging or hitting, skin cutting, or finger biting and includes ocular, genital and oral self-mutilation. Lesch-Nyhan syndrome is a rate X-linked recessively inherited disorder, caused by complete absence of hypoxanthin-guanine phosphoribosyl transferase(HPRT) activity. Clinical presentation is characterized by mental retardation, chorea, athetosis, hyperuricemia, uricosuria and self-mutilating behavior. In these patients, the most typical feature is loss of tissue from biting themselves, even though they are not insensitive to pain. The dental management of self-mutilation includes treatment with appliances such as soft mouthgrard or lip bumper, extraction of all the teeth, and orthognathic surgery. We report a 25-month-old boy who was a known case of Lesch-Nyhan syndrome and presented with severe self-mutilation wound on his lower lip. Vital pulpectomy and coronal resection was done as a more conservative approach than extracting all primary anterior teeth. Due to maintaining the root portion of the teeth in the bone, it is expected that the normal growth of the alveolar bone will be achieved.

韓國産 植物凝集素 Falcata japonica K. 의 法醫學的 利用에 關한 硏究

崔衡鎭 大韓法醫學會 1988 대한법의학회지 Vol.12 No.2

In order to find our whether the extract of Korean native F.japonica which is known as A₁-lectin could be used in typing of blood stain and various body secretion and its stain for the subject of the judgement in, the field of legal-medicine, agglutinating inhibition test by human saliva, semen and vaginal secretion was performed. The results could be summarized as follows: 1. Agglutinating activity of F.japonica extract was specifically inhibited by saliva, semen, vaginal secretion of A₁ and A₁B type secretor. 2. Agglutinating activity of F.japonica extract was specifically inhibited by blood stain of A₁ and A₁B type and also by saliva stain, semen stain of secretor type. 3. Bloods of rats were classified into two groups, such as agglutinated and nonagglutinated groups by use of F.japonica extract. 4. Agglutinating activity of F.japonica extract was also specifically inhibited by use of agglutinated group of rat blood and blood stain.

小兒銀杏中毒症 1例

崔衡鎭,金榮昌 순천향대학교 1983 논문집 Vol.6 No.4

Three year-old male child was admitted with chief complains of vomiting and tonic & clonic convulsive attack after three hours taking fresh fruits of Ginkgo biloba L.. He was treated with anticonvulsive drugs and 'fluid infusion, recovered completely and discharged in fine condition two days later. Brief review of related literatures were presented.