콩 단백 성분 및 연령이 암컷 흰쥐의 혈장 지질 농도와 인지질 지방산 패턴에 미치는 영향

정은정(Eun-Jung Chung),김수연(Soo-Yeon Kim),김지영(Ji-Young Kim),안지영(Ji-Young Ahn),박정화(Jung-Wha Park),차명화(Myung-Hwa Cha),이양자(Yang-Cha Lee Kim) 한국식품영양과학회 2003 한국식품영양과학회지 Vol.32 No.2

혈중 지질농도는 식이단백질 종류보다는 연령의 영향을 많이 받아, young군보다 old군의 총 콜레스테롤, TG, HDL-콜레스테롤, LDL+VLDL-콜레스테롤 및 AI 모두 높았다. 한편 콩 단백질군에서 HDL-콜레스테롤 수준이 증가하고, LDL+VLDL-콜레스테롤와 AI는 감소하여, 심혈관질환에 대한 콩 단백질의 유익한 효과를 확인할 수 있었다. 식이단백질에 의해 유의한 영향을 받은 혈장 인지질의 지방산조성중 22:0, 18:1ω9, ∑MUFA 조성은 카제인군에서 콩 단백질군 보다 높았으며, 반대로 ∑SFA 조성은 카제인군에서 낮았다. 연령에 의해 혈장 인지질 지방산 조성에 있어 유의한 차이를 보인 지방산 중 22:0, 18:1ω9, 22:1, 18:3ω3 및 22:4ω6는 young군에서 높은 반면에, 22:6ω3, ∑ω3, 18:2ω6, 20:4ω6, ∑ω6 및 ∑PUFA의 조성비율은 old군에서 높게 나타났다. 이와 같이 식이 단백질의 종류보다 연령의 영향력이 더 많이 나타난 것은 혈중 콜레스테롤 수준이 식이 단백질보다는 연령의 영향을 더 크게 받았기 때문으로 생각된다. 혈장 인지질 지방산의 대사지표 중 Δ7-desaturation index(16:0⇒16:1ω7)와 Δ9-desaturation index(18:0⇒18:1ω9)가 식이 단백질의 영향을 받아 카제인군에 비해 콩 단백질군에서 유의하게 낮았다. Δ7- 및 Δ9-desaturation index만이 식이 단백질의 영향을 받은 것은 식이 단백질보다 desaturase 활성에 더 큰 영향력을 미치는 콜레스테롤이 식이에 첨가되었기 때문이라 생각된다. Δ-4 desaturation index (22:4ω6⇒22:5ω6)는 young군에 비해 old군에서 높았으며, elongation index(20:4 ω6⇒22:4ω6)는 old군에서 낮았다. 대부분의 elongation과 desaturation 단계는 연령에 따라 유의한 차이를 보이지 못하였으나, ω3계 지방산의 전체적인 elongation-desaturation 단계를 나타내는 ∑products-fatty acid(ω3)/α-LNA(ω3) 비율이 old군에서 young군보다 유의하게 높아, 연령에 따른 PUFA 대사의 변화가 관찰되었다. 이상에서와 같이, 콩 단백질의 섭취로 흰쥐 혈장 인지질의 ∑MUFA 조성은 낮고 ∑SFA 조성은 높아 다른 지질 강화성분의 섭취와 유사한 결과를 나타내었다. 혈중 콜레스테롤 수준이 높은 old군에서 체내 막조직의 유동성을 유지하기 위해 PUFA의 합성 특히 ω3계 지방산의 elongation-desaturation이 증가하여 총 PUFA 조성이 young군보다 높았다. 연구결과로 이소플라본을 포함한 콩 단백질의 혈중 지질저하 기전에 대해 체계적인 설명은할 수 없지만, 콩 단백질이 혈장 지방산의 조성을 변화시킴으로써 간접적으로 심혈관계 질환에 대해 유익한 효과를 미침을 알 수 있었다. 한편, 이러한 혈관의 건강과 관련된 콩의 유익한 효과는 이소플라본과 같은 콩의 특정 성분의 단독효과라기보다는 콩 단백질과의 복합효과에 의한 것이라는 주장은 매우 설득력 있다고 생각된다. Effects of soy protein concentrate (SPC) containing isoflavone and casein diets on plasma phospholipid (PLs)-fatty acid patterns were investigated in 7- & 40- wk old female rats. Diets containing 16% SPC (soy/young: SY, soy/old: SO) and casein (casein/young: CY, casein/ old: CO) supplemented with 0.5% cholesterol were fed for 4 wks. Fatty acid compositions of plasma PLs were determined by TLC and GLC. Compared to the dietary protein effects, age effects on serum lipids were more profound. The levels of total cholesterol (Chol.), triglyceride, HDL-Chol., (LDL+VLDL)-Chol. and atherogenic index (AI) were higher in older groups (OC & OS) than younger groups (YC & YS). Soy groups had higher HDL-Chol. level and lower (LDL+VLDL)-Chol. and AI, compared with casein groups. The compositions of C22:0, C18:1ω9 and sum of MUFA in plasma PLs were significantly higher in casein group (CY & CO) than soy group (SY & SO), but those of sum of SFA were higher in soy group. The compositions of C22:0, C18:1ω9, C22:1, C18:3ω3 and C22:4ω6 were higher and those of C22:6ω3, sum ofω3, C18:2ω6 C20:4ω6, sum ofω6 and sum of PUFA were lower in plasma PLs of younger rats. The average P/S and ω3/ω6 ratio in older group was higher. The Δ-7 desaturation index (16:0⇒16:1ω7) and Δ-9 desaturation index (18:0⇒18 : 1ω9) were lower in soy group than casein group, while Δ-6 and Δ-5 desaturation index were not affected by dietary protein. The Δ-4 desaturation index (22:4ω6⇒22:5ω6) were higher and, elongation index (20:4ω6⇒22:4ω6) were lower in older group. The ratio of the products of ω3 fatty acid series/precursor of ω3 fatty acid series (C18:3) was significantly higher in older group, which in- dicated that age affected the plasma PUFA metabolism. On the other hand, older rats had higher serum cholesterol level compared with younger rats. Taken together, these changes in fatty acid composition might cause minimal changes in the membrane fluidity induced by the increase serum cholesterol level.

위암의 연령에 따른 차이

김영재(Young Jae Kim),이영철(Young Chul Rhee),이만길(man Kill Lee),차호(Ho Cha),정영채(Young Chae Jung) 대한소화기학회 1987 대한소화기학회지 Vol.19 No.1

N/A Comparative analytic review on clinical, endoscopic and histopathological findings was done in 13 cases of young age (under 29), 446 cases of mid age (30~69) and 39 cases of old age (above 70) stomach cancer who were visited Department of Internal Medicine of Masan Koryo General Hospital from March 1981 to July 1985. The following results were obtained: The peak of age incidence was the 5th decade amounting to 36.8%. The ratio between male and female was 2.6:1. The main chief complaint of each group was epigastric pain and it was followed by epigastric fullness. The most patient had the duration of symptom from 3 months to 6 months. For old-age group, mid-age group, major site of lesion in the antrum, meanwhile body for young-age group. The most frequent type of gastric cancer was type III in each group by Borrmanns classification. For each group, adenocarcinoma was major cases in the view of histopathological findings, and incidence of well differentiated type adenocarcinoma were lower in young-age group than others.

노화가 인체 중간엽 줄기세포로부터 조골세포로의 증식 및 분화에 미치는 영향

백기현,태현정,오기원,이원영,조정기,권순용,강무일,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.3

연구배경: 일반적으로 골다공증과 연관된 위험인자로는 연령, 폐경, 약물, 불충분한 칼슘섭취, 만성질환 및 운동부족 등이 있는데, 특히 노화가 진행할수록 골밀도가 감소하는 것은 잘 알려져 있다. 노화와 관련하여 진행되는 골소실은 조골세포 및 전구조골세포의 기능적 결핍에 의한 골형성의 감소가 주요한 요인으로 여겨지고 있다. 그 동안 연령이 조골모 세포의 양과 조골모 세포로부터 성숙조골세포로의 분화 및 증식에 미치는 영향에 대한 일부 보고들이 있었으나 아직 일치된 견해는 없는 형편이다. 방법: 다양한 연령의 사람으로부터 골수를 채취, 중간엽 줄기세포가 포함된 단핵세포를 분리한 후 조골세포로 분화하기 좋은 조건하에서 배양하였다. 대상군은다시 젊은군과 노령군으로 구분하여 다양한 변수를 비교 분석하였다. 일차배양에서는 CFU-F를 계수하여 골수내 중간엽 줄기세포의 수를 추산하였고, 칼슘측정을 통하여 기질의 무기화 정도를 비교하였다. 계대배양후 이차배양에서는 시기별로 알카리성 포스파타제 활성도를 측정하고 오스테오칼신 mRNA의 발현을 관찰하여 젊은군과 노령군 사이의 증식능 차이를 비교하였다. 또한 이차배양 시기별로 MTT 측정을 하여 양군간에 증식능 차이가 있는지 알아보았다. 결과: 1. 일차배양 15일째에 평균 CFU-F의 수는 젊은군에서 유의하게 많았다(젊은군 148.3±28.9, 노령군 54.3±9.1, p=0.02). CFU-F의 평균면적은 젊은군에서 넓은 경향을 보였으나 통계적으로 유의하지는 않았다. 2. 일차배양 17일 경과 후 양군간에 기질 칼슘 침착정도는 유의한 차이를 보이지 않았다(젊은군 103.6±50.6, 노령군: 114.0±56.5, p=NS). 3. 이차배양 10일째에 젊은군에서 알카리성 포스파타제 활성도가 고령군에 비해 유의하게 높았다(젊은군: 935.5±115.0 U/mg, 노령군: 578.4±115.7U/mg,p.0.05). 고령군에서는 시간 경과에 따른 변화가 미약했으며 전반적으로 알카리성 포스파타제의 활성도가 젊은군에 비해 낮았다. 4. 이차배양도중 오스테오칼신 mRNA의 발현은 배양시기별로 젊은군에 비해 고령군에서 더 낮은 경향을 관찰할 수 있었다. 5. 이차배양 10일과 15일에 젊은군에서 노령군보다세포증식이 유의하게 증가된 양상을 보였다(10앓 젊은군 0.73±0.05, 노령군 0.58±0.04, p=0.05, 15일; 젊은군 0.80±0.05, 노령군 0.70±0.03, p=0.05).결론: 이상의 연구에서 저자들은 노령군에서 젊은군보다 골수 내 중간엽줄기세포의 수가 적고, 노령군에서 유래한 전구조골세포의 성숙조골세포로의 증식 및 분화가 젊은군 보다 감소해 있는 것을 관찰할 수 있었다. Background: Osteoblasts originate from osteoprogenitor cells in bone marrow stroma, termed mesenchymal stem cells (MSCs) or bone marrow stromal cells. Each MSC forms colonies (colony forming units-fibroblasts [CFL-Fs]) when cultured ex vivo. There are some reports about the age-related changes of the number and osteogenic potential of osteoprogenitor cells, but any relationship has not been clearly established in humans. In this study, we counted MSCs using CFU-Fs count and examined the proliferative capacity and differentiation potential of osteoprogenitor cells. Finally, we analyzed how these parameters varied with donor age. Methods: Bone marrow was obtained from the iliac crest of young (n=6, 27.2±8.6 years old) and old (n= 10, 57.4k6.7 years old) healthy donors. Mononuclear cells, including MSCs, were isolated and cultured in osteogenic medium. In primary culture, we compared the colony-forming efficiency of MSCs between the two groups and determined the matrix calcification. When primary culture showed near confluence, the cells were subcultured. Alkaline phosphatase activity, osteocalcin expression by RT-PCR and proliferative potential by MTT assay were examined by the time course of secondary culture. Results: At the 15th day of primary culture, the mean number of CFU-Fs was significantly higher in the younger donors (young: 148.3±28.9, old: 54.3±9.1, p=0.02) and the mean size of CFL-Fs was also larger in the younger donors than the older donors. However, matrix calcification was not different between the two groups (young: 103.6±50.6, old: 114.0±56.5, p=NS). In secondary culture, alkaline phosphatase activities were significantly lower in the older donors. The younger donors showed peak alkaline phosphatase activity at day 10, while the older donors didn't showed a remarkable peak (young: 935.5±115.OU/mg, old: 578.4±115.7U/mg, p<0.05). Total cell number as a proliferative index increased progressively during the secondary culture and a significantly greater cell number was noted in the younger donors. Osteocalcin expression was generally upregulated in the younger donors, but this was not statistically significant. Conclusion: Our study shows that the number of osteoprogenitor cells is decreased during aging and that the proliferative capacity and differentiation potential of osteoprogenitor cells seem to be reduced during aging (J Kor SOC Endocrinol 18:296-305, 2003).

Gender-related Differences in Management of Nonvalvular Atrial Fibrillation in an Asian Population

Lee, Jung Myung,Kim, Tae-Hoon,Cha, Myung-Jin,Park, Junbeom,Park, Jin-Kyu,Kang, Ki-Woon,Shim, Jaemin,Uhm, Jae-Sun,Kim, Jun,Park, Hyung Wook,Lee, Young Soo,Choi, Eue-Keun,Kim, Chang-Soo,Joung, Boyoung,K The Korean Society of Cardiology 2018 Korean Circulation Journal Vol.48 No.6

<P><B>Background and Objectives</B></P><P>Gender-related differences in health care utilization for atrial fibrillation (AF) are increasingly recognized. However, large cohort data for examining gender-related differences in AF are lacking in Asian populations.</P><P><B>Methods</B></P><P>The Registry for Comparison Study of Drugs for Symptom Control and Complication Prevention of AF (CODE-AF Registry) is a prospective observational cohort-study that enrolled participants at 10 tertiary hospitals in South Korea. Baseline characteristics retrieved from the CODE-AF Registry were analyzed.</P><P><B>Results</B></P><P>A total of 6,274 patients were recruited (mean age 67±11 years, mean CHA<SUB>2</SUB>DS<SUB>2</SUB>-VASc score 2.7±1.7, 63% male, 65% paroxysmal AF) from June 2016 to April 2017. Women underwent less electric cardioversion (12.3% vs. 19.6%, p<0.001), less radiofrequency ablation (12.4% vs. 17.9%, p<0.001), and less antiarrhythmic drug therapy (44.7% vs. 49.5%, p<0.001), despite having more severe symptoms (symptom class III or IV, 45.8% vs. 37.5%, p<0.001). Among patients with a CHA<SUB>2</SUB>DS<SUB>2</SUB>-VA score of 2 or more, a slightly higher proportion of women were taking oral anticoagulants than men (85.7% vs. 81.9%, p=0.002), and non-vitamin K antagonist oral anticoagulant (NOAC) use was more prevalent in women than men (70.4% vs. 62.3%, p<0.001). Insufficient NOAC dosing was very common, more so in women than men (61.5% vs. 56.3%, p<0.001).</P><P><B>Conclusions</B></P><P>Female patients with AF were treated more conservatively and rhythm control strategies were used less frequently than in males, even though the female patients with AF had more severe symptoms. While insufficient NOAC dosing was common in both sex, it was significantly more frequent in women.</P>

Optimal Fermentation Conditions for Enhanced GlutathioneProduction by Saccharomyces cerevisiae FF-8

Jae-Young Cha1,,Jin-Chul Park,Beong-Sam Jeon,Young-Choon Lee,Young-Su Cho 한국미생물학회 2004 The journal of microbiology Vol.42 No.1

The influence of feedstock amino acids, salt, carbon and nitrogen sources on glutathione production by Saccharomyces cerevisiae FF-8 was investigated. Glucose, yeast extract, KH2PO4, and L-cysteine were found to be suitable feedstock. Highest glutathione production was obtained after cultivation with shaking for 72 h in a medium containing glucose 3.0% (w/v), yeast extract 3.0%, KH2PO4 0.06% and L-cysteine 0.06%. The glutathione concentration achieved using this medium increased 2.27-fold to 204 mg/l compared to YM basal medium.

Real-World National Colonoscopy Volume in Korea: A Nationwide Population-Based Study over 12 Years

Jae Myung Cha,Min Seob Kwak,Hyun-Soo Kim,Su Young Kim,Sohee Park,Geun U Park,Jung Kuk Lee,Soo Jin Kim,Hun Hee Lee,Joo Sung Kim,Won Ho Kim,Big Data Research Group of the Korean Society of Gastroenterol 거트앤리버 소화기연관학회협의회 2020 Gut and Liver Vol.14 No.3

Background/Aims: Little is known about the national colonoscopy volume in Asian countries. This study aimed to assess the national colonoscopy volume in Korea over a 12-year period on the basis of a nationwide population-based database. Methods: We conducted a population-based study for colonoscopy claims (14,511,158 colonoscopies performed on 13,219,781 patients) on the basis of the Korean National Health Insurance Service database from 2002 to 2013. The 12-year national colonoscopy burden was analyzed according to patient age, patient sex, and healthcare facility type. Results: The overall volume of colonoscopy increased 8-fold over the 12-year period. The annual colonoscopic polypectomy rate significantly increased in all patient sex and age groups over the 12-years period (all p<0.001). The yearly colonoscopic polypectomy rate for men was significantly increased compared with that for women (2.3% vs 1.7%, p<0.001) and for the screening-age group compared with that for the young-age group (2.0% vs 1.6%, p<0.001). The yearly colonoscopic polypectomy rate relative to the total colonoscopy volume significantly increased in primary, secondary, and tertiary facilities by 2.4%, 1.9%, and 1.4% during the 12-year period (all p<0.001). In addition, the annual colonoscopy volume covered by high-volume facilities significantly increased by 1.8% in primary healthcare facilities over the 12-year period (p<0.001). Conclusions: Healthcare resources should be prioritized to allow adequate colonoscopic capacity, especially for men, individuals in the screening-age group, and at primary healthcare facilities. Cost-effective strategies to improve the quality of colonoscopy may focus on primary healthcare facilities and high-volume facilities in Korea.

한국인 인슐린 비의존형 당뇨병 및 이차성 당뇨병 환자에서 글루코키나제 유전자 변이

남재현(Jae Hyun Nam),이현철(Hyun Chul Lee),김연의(Youn Euy Kim),권석호(Suk Ho Kwon),윤용석(Yong Suk Yoon),박석원(Suk Won Park),원영준(Young Jun Won),차봉수(Bong Su Cha),송영득(Young Duk Song),이은직(Eun Jig Lee),임승길(Sung Kil Lim) 대한내과학회 1998 대한내과학회지 Vol.54 No.6

N/A Objectives: Mutations in the glucokinase (GCK) gene are considered a possible cause of maturity-onset diabetes of the young. The purpose of this study was to evaluate the contribution of this gene to the development of non insulin dependent diabetes mellitus (NIDDM), gestational diabetes mellitus (GDM) and post-renal transplantation diabetes mellitus (PTDM). Method: Identification of GCK mutation was attempted on 39 NIDDM patients, 2 GDM patients and 58 selected renal allograft recipients with PTDM and 45 normal controls. The exons in the GCK gene were examined by polymerase chain reaction (PCR), followed by analysis of single-stranded DNA conformational polymorphism (SSCP). The abnormal bands were also confirmed by DNA sequencing analysis. The exons of affected family members were also investigated for mutations of the GCK gene. Results: Two of the 58 PTDM patients (3.4%) were found to have CKK mutations. One had the mutation on exon 5 and the other on intron 7. One control subject had the mutation on intmn 9. The mutation of exon 5 was identified as a substitution of CCT (proline) for CTT (leucine) at codon 164, which has not ever reported before. The family members of the PFDM patient with mutation of exon 5 were analyzed by PCR followed by SSCP, and two of them revealed the same mutation. The abnormal band on the SSCP analysis of exon 7 was identified as the insertion of base C/T at the 39th nucleotide in intron 7. Two family members of this patients also had same band on SSCP. The one mutation of 45 normal controls was CT located at the 8th nucleotide in intron 9, which was a common polymorphism. Conclusion: We found GCK mutations in subjects with PTDM and we speculate that these mutations may be one of the contributing cause of PTDM.

가평 전주이씨묘 출토복식 고찰

최연우,박윤미,김윤경,김지희,박양희,이선우,차서연,편나영,황혜남,Choi, Yeon Woo,Park, Yoon Mee,Kim, Yoon Gyung,Kim, Ji Hee,Park, Yang Hee,Lee, Seon U,Cha, Seo Yeon,Pyeon, Na Young,Hwang, Hye Nam 한국의류학회 2017 한국의류학회지 Vol.41 No.5

In 1995, the costumes were restored in an unknown woman's tomb at the Jeonju Lee's family cemetery in Gapyeong, Gyeonggi-Do, and there are currently nine items remaining. In this study, we first introduced these 9 relics to academia and analyzed the morphological characteristics of the costumes. We also estimated the time and person of burial compared with costumes unearthed from other burials. Jeogories (short jacket) are all four items. One of these items was unusual in shape, and the upper part of the seop (gusset) was wrinkled and shaped. This type of Jeogori appears only till the 1520s in other tombs. There are also two skirts, one of which is characterized by a superimposed pattern. The top and bottom / middle part of the skirt were rolled up, and the skirt, which was double-rolled up in this way, was first discovered. An analysis of the shape of Jeogori and skirt indicated that the person buried in the Jeonju Lee's family tomb was believed to have survived until the early 16th century. Based on these estimated periods, as a result of looking at the genealogy of Jeonju Lee's family, it was concluded that the tomb was probably a woman named Lee Geum Myeong (李金命) born in the mid-15C and died between the late 15C and the early 16C.

만곡수로에서 교각으로 인한 수위변화 해석

차영기,김이현,이만석 단국대학교 신소재기술연구소 2000 신소재 Vol.9 No.-

본 연구는 만곡수로에서의 교각과 유량변화데 따른 수이변화의 특성을 실험으로 고찰한 것이다. 고정상과 이동상의 경우에 교각으로 인한 배수위 상승의 크기와 영향을 비교ㆍ분석함으로서 최대 상승량을 일으키는 지점의 변화와 그 원인을 알아보았다. 만곡부에서 교각 수와 그 크기를 달리하고, 그에 따른 배수위 상승 영향을 알아보았다. 만곡수로의 흐름을 관찰한 결과 최대 수위를 나타내는 지점은 고정상과 이동상에서 각각 단면 19와 단면 10에서 나타났고, 편수위 상승은 고정상에서 단면 6에서 최대이고, 이동상에서는 단면 9에서 최대로 나타났다. 유량 변화에 따른 최대 배수위 상승량은 평균적으로 고정상에서는 교각 설치 단면의 상류 첫 번째 단면에서, 이동상에서는 교각 설치단면의 상류 세 번째 단면에서 발생하였다. 또한 유량 변화량의 평균값인 1400CMS 유량으로 반복 실험한 결과 유수단면적을 달리할 때, 즉 교각 수와 교각 지름의 변화에 따라 고정상에서는 교각 설치 단면의 상류 세 번째 단면에서, 이동상에서는 교각 설치 단면에서 최대 배수위 상승이 발생하였다. This paper investigate the characteristics of water surface level due to piers and due to varying discharge in a curved channel by experiment. It is compared and analyzed the amount and effect of backwater due to piers, and the variation of the highest backwater points in a fixed bed and a movable bed respectively. And the variation of flow varying numbers and diameter of the pier at the bending part. The highest stage in a curved channel is showed at the section of 19 and 10 in the cases of a fixed bed and a movable bed respectively. The largest superelevations at a fixed bed and a movable bed are showed at the section of 6 and 9 respectively. The highest backwater stage due to varying discharge at a fixed bed is brought out at the first upsteam section of pier setting, and at he case of a movable bed it was the third upstream section of pier setting. Also, the highest backwater stage is brought out at the third upstream section of pier setting in a fixed bed, and at section of pier setting in a movable bed at the case of varying the pier numbers and diameter for 1,400CMS.

Gitelman씨 증후군 1예

차영학,송명준,신요식,김성민,김영욱,김윤권,김소연,김영중,조민구,이권전 全南大醫大 2001 전남의대학술지 Vol.37 No.3