넙치, Paralichthys olivaceus 치어의 생존, 성장 및 산소소비에 미치는 수은의 만성적 독성

강주찬,황운기,지정훈,김성길,김재원 한국어병학회 2002 한국어병학회지 Vol.15 No.1

산업활동의 증가로 인해 해양에 유입되는 수은 (Hg)이 저서성 연안정착 어류인 넙치, Paralichthys olivaceus 치어의 생존, 성장, 사료효율 및 산소소비에 미치는 영향을 조사하였다. 6주 동안 0~0.13mg/L의 아치사 농도에서 넙치를 사육하며 Hg 독성에 대한 영향을 조사하였다. Hg 농도와 노출기간이 증가할수록 생존육은 감소했으며 6주 후, 0.05mg/L 이상의 농도 구에서 생존율이 급격히 감소했다. 성장률과 사료효율 또한, 각각 0.028mg/L (P<0.01)와 0.05mg/L (P<0.01) 이상의 농도 구에서 현저하게 감소하였다. 산소소비율은 0.05mg/L와 0.13mg/L의 농도구에서 각각 대조 구에 비해 25(P<0.01)와 32% (P<0.01) 감소해, 유의적인 차이를 나타내었다. 이상의 결과로부터 Hg 독성은 넙치의 성장, 사료효율 및 산소소비와 같은 생리적 기능을 저해해 생존율을 감소시키는 것으로 나타났다. Effect of mercury (Hg) toxicity on survival, growth, feed efficiency and oxygen consumption were examined in the juvenile olive flounder. Paralichthys olivaceus. Fishes were exposed to sublethal concentrations of Hg ranging from 0 to 0.13mg/L for 6 weeks. Hg reduced survival rate in a concentration and exposure period-dependent way and suddenly reduction occurred at Hg concentrations greater than 0.05mg/L after 6 weeks. Growth rate and feed efficiency also significantly decreased at greater than 0.028 and 0.05 mg/L respectively. Oxygen consumption rate was significantly decreased to 25 and 32% than that of the control at the Hg concentration of 0.05 and 0.13 mg/L respectively. These results suggest that Hg toxicity inhibit physiological function including growth, feed efficiency and oxygen consumption in the juvenile olive flounder, resulting in survival failure at high concentration.

TBT의 노출에 따른 넙치, Paralichthys olivaceus의 생존, 성장 및 산소소비의 변화

강주찬,황운기,지정훈 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.3

This study was carried out to examine the effects of his (tribytyltin)oxide (TBT), endocrine disrupting compounds (EDCs), on the changes of survival, growth and oxygen consumption rate in the oliver flounder, Paralichthys olivaceus. Oliver Flounders were exposed to sublethal concentration of TBT (0, 1.67, 3.20, 6.30 and 12.50㎍/L) during 6 weeks. Survival rate was decreased in a concentration and exposure period-dependent way and suddenly the reduction of more than 20% occurred at TBT concentration greater than 3.20㎍/L after exposure 6 weeks. Growth rate and feed efficiency significantly decreased at concentration greater than 3.20㎍/L. Oxygen consumption rate was also decreased in a concentration-dependent way and significantly decreased to 17, 48 and 67% than that of the control at the TBT concentration of 3.20, 6.32 and 12.50㎍/L, respectively. This study revealed that high TBT concentration (≥ 3.20㎍/L) reduced growth and oxygen consumption rates of the juvenile oliver flounder suggesting potential influence on the natural mortality of Paralichthys olivaceus in the coastal areas.

양식장 배출물 발효물의 어류 사료 첨가에 따른 넙치, Paralichthys olivaceus의 혈액학적 변동에 미치는 영향

강주찬,지정훈,송승엽,문상욱,강지웅,이영돈,김세재 한국어병학회 2004 한국어병학회지 Vol.17 No.1

본 연구는 우리나라 주요 양식어류인 넙치, Paralichthys olivaceus를 대상으로 육상수조식양식장 배출물 발효물의 어류 사료 첨가에 따른 혈액학적 변동에 미치는 영향을 검토하였다. 젖산균 및 효모를 사용하여 발효한 양식장 배출물의 이화학적 분석을 실시하였고, 발효물을 0.1, 0.5, 1.0 및 5.0% 농도로 8주간 투여하였다. 투여 4주째 및 8주째에 각 투여 구간별로 혈액학적 항목을 중심으로 그 차이를 확인하였다. 배출물 발효농도별 유의한 차이는 나타나지 않았지만, 분석시기에 따른 적혈구수, 혈색소 농도 및 혈색소 지수가 증가하는 경향을 나타내었으나, 주요 혈청 무기 및 유기성분은 배출 발효물 농도별로 유의한 차이는 나타나지 않았다. 또한 혈청 효소학적 조사에서는 조사시기별로 변동은 나타났지만, 동일 조시시기에서 각 구간별 유의한 차이는 나타나지 않았다. 따라서 이러한 육상 약식장 유래의 배출물 발효산물에 대한 다양한 방면의 연구가 진행되어야겠다. Effects of oral administration with fermented product from sewage in land-based seawater fish farm on haematological disturbance in the olive flounder, Paralichthys olivaceus was investigated. After 4 weeks of conditioning with a basal diet, fish were divided into 4 groups and provided experimental diet (0.1,0.5, 1.0 and 2.0%) supplement of fermented sewage for 80 days. Proximal analysis was performed for the product of sewage which was fermented by lactic acid and yeast. RBC count, hemoglobin concentration and hematocrit value were increased according to the treated periods, however, no statistical difference was observed between control and treatment groups. There were no significant difference in serum organic, inorganic compounds and enzyme activities between control and treatment groups. This study hypothesized that the supplement of fermented product from sewage in land-based seawater fish farm might be an additive sup plement for source of fish diet in view of haematological examination. Recycling of the sewage may be an economic artificial sources of diet for fish aquaculture practices.

지난 2년간의 학회지 "신경정신의학" 심사 평가보고

김찬형,김세주,김도관,김성곤,박원명,송동호,박용천,이영문,오강섭,정영철,이상열,김주한,이동영,이헌정,함병주,김율리,김문두,김정진,이강욱,천근아,김재진,홍진표,홍승철 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.6

조혈모세포이식환자에서 조혈기능의 회복과 관련된 인자들에 관한 연구

정기주,강명수,권기두,김경하,이상철,김현정,배상병,김찬규,이남수,이규택,원종호,홍대식,박희숙 순천향대학교 의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.1

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ESPI 기법에 의한 하중을 받는 균열 박판의 진동 특성에 관한 연구

김경석,강기수,최지은,박찬주,홍진후 한국비파괴검사학회 2001 한국비파괴검사학회지 Vol.21 No.2

대부분의 실제구조물은 인장과 진동 등이 동시에 작용하는 복합하중 상태가 되며 이러한 환경에서 물체의 거동을 해석하는 것이 중요하다. 이 논문에서는 χ축에 대해 45˚결함이 있는 평판이 하중을 받고 있을 때, 진동 특성을 시간평균 electro speckle pattern interferometry(ESPI)를 이용하여 해석하였다. 결함이 있는 박판 시험편에 인장력을 증가하면서 물체의 고유진동수 변화와 모드의 형태 변화를 동시에 관찰하였다. 실험결과에서 결함은 저차모드에서는 진동모드에 영향을 주지 않았으나, 고차모드에서는 진동모드에 변화를 주었으며, 특정 진동모드에서 결함의 영향으로 현저하게 고유주파수가 낮아지는 경향을 보였다. 또한 실험결과는 유한요소해석과 비교하여 주파수 비교에서는 5%이내의 오차율을 보였으며, 진동모드 비교에서는 완전히 일치하였다. This paper presents the vibration characteristics of a rectangular plate with 45˚ oblique crack subjected to a uniaxial tension. The experiment is adopted by the time-average Electronic Speckle Pattern Interferometry(ESPI) method. The natural frequency and mode shape are considered accurately according to the increase of tensile load. When tensile load is zero, the vibration modes are agreed with the smooth and the 45˚ obliquely cracked plate. But according to the increasement of load, it is shown that vibration modes are extremely varied. The effects of the crack under the vibration are discussed in detail. It is indicated that the increase of load makes the variation of the frequencies and modes complicate in the range of even a small load. The results are agreed with the FEM analysis within 5%.

외인성 Estrogen에 노출된 조피볼락, Sebastes schiegeli 치어의 혈장 VTG과 GPT의 변화

황운기,강주찬 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.3

Changes of plasma vitellogenin (VTG) and glutamate pyruvate transaminase (GPT) were examined for determining whether hepatocyte was damaged during the process of VTG induction in the juvenile rockfish, Sebastes schlegeli exposed to exogenous estrogen (estradiol-17β, E₂). Rockfishes were intraperitoneally injected with E₂(5 mg/kg B.W.) in 70% ethanol and plasma sampling were extracted at 0, 1, 3, 6, 9, 12, l5 days after E₂ administration. VTG and GPT were then analyzed by SDS-PAGE and Reitman-Frankel method, respectively. VTG band was detected at a molecular weight position of 175 kDa on Day 3 after E₂ administration. This band became more distinct at 6 days, but its was gradually thinned with time-course, and not detected at 15 days. GPT was suddenly increased at 1 days after E₂ administration and highest GPT was detected at 3 days. However, OPT was gradually decreased with time-course as the change of VTG. These results suggest that the process of VTG induction by exogenous E₂ damage to hepatocyte, and plasma GPT was temporarily increased in the juvenile rockfish.

수은, 납 및 구리에 대한 붕어(Carassius auratus)와 돌고기(Pungtungia herzi) 자어의 급성독성

조규석,박종호,김주찬 한국물환경학회 2004 한국물환경학회지 Vol.20 No.3

Larva of Carassius auratus and Pungtungia herzi were exposed to mercury (HgCl₂), lead (PbCl₂) and copper (CuSO₄) to determine acute toxicity. The toxicity tests were conducted triplicate and the LC_(50) values (24, 48, 72 and 96 hours) were determined for two species. Data obtained from the toxicity tests were evaluated using the probit analysis. Although sensitivities of two species to mercury were relatively similar, C. auratus was more susceptible than P. herzi to the exposure of lead but P. herzi was more sensitive than C. auratus on copper. The sensitivity on three metals tested may be ranked in the following order from highest to lowest toxicity on larval stage of these fish: mercury > copper > lead. It is suggested that acute toxic test at the larval stage of C. auratus and P. herzi, indigenous species in Asia area, is an important part of the ecotoxicological assessment of some heavy metals.

유기주석 노출에 의한 Mysid, Neomysis awatschensis의 독성 영향

지정훈,김상규,황운기,강주찬 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.4

Tests for the toxicity of tributyltin (TBT) were conducted on mysid collected from Dadepo beach, Pusan, Korea. The toxic effects of tributyltin on the survival, growth and oxygen consumption of the mysid, Neomysis awatschensis have been evaluated. Mysids were exposed to several concentrations of TBT (0, 0.56, 1.15, 3.07 and 6.12 ㎍/L) for 6 weeks. Survival rate was decreased with increases in concentration and exposure time and the reduction of more than 40% occurred at TBT concentration greater than 1.15 ㎍/L after 6 weeks. Growth rate was significantly decreased at concentrations greater than 1.15 ㎍/L Oxygen consumption rate was also decreased in a concentration-dependent way and significantly decreased to 39, 47 and 69% of the control at 1. 15, 3.07 and 6.12 ㎍/L, respectively. These results indicate that the contamination of aquatic environment by TBT has the potential to significantly reduce coastal and estuaries recruitment of mysid.

Pb의 노출에 따른 넙치, Paralichthys olivaceus, 치어의 생존, 성장 및 산소소비율의 변화

김성길,김재원,장석우,김상규,강주찬 한국어병학회 2002 한국어병학회지 Vol.15 No.3

Experiments were carried out to investigate the effects of lead exposure on survival, growth and oxygen consumption of juvenile oliver flounder, Paralichthys olivaceus. Survival rate of the oliver flounder was significantly affected above 150㎍/L. Growth of the olive flounder exposed to ≥ 150 ㎍/L of lead concentration was significantly lower than that of fish reared in normal condition. Metabolic rate of fish exposed to lead ≥ 80 ㎍/L concentration was significantly reduced with increasing lead concentration than that in normal condition. This study revealed that high lead concentration (≥ 80 ㎍/L) reduced growth and metabolic rates of juvenile olive flounder suggesting potential influence of lead on the natural mortality of olive flounder in the coastal areas.