mecA 양성 메티실린 내성 포도구균의 mec 조절유전자에 대한 분자역학적 연구 및 다양성 분석

우희연,이남용,맹성호,한승훈,인경수,김상욱,성승용,김익상,최명식 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.3

목적 : 병원 감염과 지역 감염의 원인균으로서의 중요성이 증가되고 있는 MRSA와 MRCNS의 메티실린 내성 관련 유전자에 대해 분자역학적인 조사를 시행하고 mec 조절유전자의 다양성을 분석하여 분자유전학적 연구와 감염 관리에 기본적 자료를 제공하고자 연구를 시행하였다. 방법 : 본 연구에서는 국내 동일 대학병원에서 분리된 mecA 양성 MRSA 78균주와 MRCNS 36균주를 대상으로 항균제 감수성 검사와 중합효소연쇄반응을 시행하여 표현형과 유전형의 분포를 알아보고 내성 정도와 유전형의 상관성을 분석하였다. 결론 : 최소억제농도를 측정하여 oxacillin 감수성 양상을 알아본 결과 총 90개의 MRSA 임상검체 중 3균주에서 감수성을 보였고, MRCNS 36균주는 모두 내성을 보였으며, 고도 내성을 보이는 균주일수록 다제내성을 보이고 있었다. 중합효소연쇄반응으로 mecA 유전자 유무를 분석하여 mecA 양성을 보인 MRSA 78균주와 MSCNS 36균주 만을 대상으로 mec 조절유전자에 대한 중합효소연쇄반응을 시행하여 유전형의 분포를 조사하였다. 그 결과 MRSA와 MRCNS 모두에서 mecA-Pr-mecA-mecR1-mecI형, mecA-Pr-mecA-mecR1형과 mecA-Pr-mecA-mecR-1의 5′말단형의 3가지 유전형이 공통적으로 관찰되었는데, 이외에 MRSA에서는 mecA-mecR1형, mecA-mecR1의 5′말단형이, MRCNS에서는 mecA-Pr-mecA형과 mecA형이 추가로 분리되었다. 유전형의 분포를 연도별로 분석한 결과 연도별로 우세한 유전형이 서로 다른 것을 알 수 있었다. 유전형과 내성 정도의 상관성을 분석한 결과, mecA-Pr-mecA-mecR1을 보유한 MRSA의 경우 mecA 억제유전자로 생각되는 mecI 유전자 유무에 관계없이 모두 고도 내성을 나타내어 mecI의 유무는 내성 정도와는 일정한 관련이 없었으나, MRSA와 MRCNS 모두에서 mecI와 동시에 mecR1의 3′말단이 결혼된 유전형이 저도 및 중등도 내성을 보이는 균주에서 유의하게 많이 관찰되어 mecI가 결손된 균주에서는 mecR1의 3′말단부위의 유무가 내성 정도의 결정에 중요한 역할을 할 것으로 생각하였다. 결론 : 국내에서 분리되는 메티실린 내성 포도구균의 유전형의 분포가 국외 분리주와 차이를 보이는 것으로 관찰되었다. 또한 기존의 보고와는 달리 메티실린 내성 정도와 유전형 사이에 유의한 연관성이 있는 것으로 관찰되어 mec조절유전자가 내성 발현 뿐만 아니라 내성 정도의 결정에 중요한 역할을 하는 것으로 관찰되었다. MRSA가 중요한 감염균임에도 불구하고 아직까지 MRSA의 내성기전에 대해 확실하게 규명되지 않아 치료가 더욱 어려워지고 있는 실정이므로 이에 대한 더욱 종합적인 연구와 동시에 발생과 확산을 막기 위한 노력이 필요할 것으로 생각된다. Background : High prevalence of methicillin resistance has been noticed in staphylococci which also have been recognized as important nosocomial and multi-drug resistant pathogens. In this study, we investigated the distribution of mec regulator genes and the presence of the mutation in mecIgene to reveal the resistance mechanism at molecular level. Methods : The isolates included 90 clinical isolates of MRSA, 36 MRCNS of which methicillin resistance were determined by disk diffusion test and isolated in a single hospital during 1996-1999. We performed microdilution MIC test for oxacillin resistance and Kirby-Bauer test for other antibiotics. Genotypes of mecA positive (determined by PCR) isolates (78 MRSA, 36 MRCNS) were investigated by PCR amplification of mec regulator genes such as 3' end and 5' end of mecR1. mecI, and Pr-mecA. Results : In MIC assay, all MRSA strains except 3 strains and all MRCNS strains showed oxacillin resistance. The level of resistance correlated to the multi-drug resistance. Seven genotypes were observed in mecA positive MRSA and MRCNS. Differences of predominant genotypes among years isolated and among species were observed. Deletions of the 3' end of mecR1 and the mecI were more frequently observed in the low- and borderline-level resistant MRSA and MRCNS (P(0.05). So the important role of 3' end of mecRI in determining resistance level was suggested. Conclusion : This study demonstrated that genomic distribution of methicillin-resistant staphylococci isolated in Korea was different from that of strains isolated in other countries. And important role of mec regulator genes for expression of methicillin resistance was suggested.

投砲丸에 있어서 身體分節의 運動量에 關한 硏究

崔大雨,白明基 忠南大學校體育科學硏究所 1983 體育科學硏究誌 Vol.1 No.1

The present study was performed to see the momentum of body segment in Shot-put throw. A female athlete of Chungnam National University who was a National junior athlete of Shot-put was served as subject. The analysis of the subject’s shot-put throwing motion is as follows: 1. The record which was gained by O’Brien Method is 13m20cm(Total momentum). That is, Throwing at the original place as Leg strength is the best record(43.03%), the Power of Arm stretching 22.88%, turning of shoulder 17.35%, Gliding 9.85%, Snapping 6.89%. 2. To gain the better record, Shot-putter should build up the Power of Gliding and Snapping through partial practice. 3. To improve Physical fitness, Shot-putter should build up the Power of Leg and Body Upper body through Weight training, Speed, and Jumping exercises.

재조합 균주 Bacillus subtilis LKS88에 의한 Streptomyces albus KSM-35 Amylase의 생산조건

최원진,유도진,이재우,소명환,김영배 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.4

Streptomyces albus KSM-35의 amylase의 유전자를 Bacillus subtilis LKS88에서 발현시킨 균주를 사용하여 amylase의 생산에 미치는 몇가지 요인들을 조사하였다. 본 실험에 사용한 탄소원들중에서 sodium citrate와 왕겨의 효소 생산량이 가장 높았고, 이들을 1:1의 비율로 혼합하여 첨가하였을 때 효소의 생산량은 대조군으로 사용한 가용성 전분에 비하여 20배 이상 증가하였다. 질소원으로 대두박을 사용하였을 경우 yeast extract의 첨가량을 줄이면서 효소의 생산량을 증가시켰다. 배양을 위한 초기 pH를 60으로 조절하고 SDS를 0.01%첨가하였을 때 대조구조보다 높은 효소 생산량을 나타내었다. Bacillus subtilis LKS88 (pASA240)을 sodium citrate와 왕겨 각각 1.5%, K_2HPO_4 0.66%, yeast extract 0.3%, 대두박 0.7%, SDS 0.01% 및 초기 pH를 6.0으로 조절한 배지에서 37℃에서 배양시 최대 효소 활성은 배양 후 36시간에서 나타났으며 이때 효소 활성은 56.6 U/ml이었다. The effects of culture conditions on the production of amylase expressed by Bacillus subtilis LKS88 with a cloned gene from Streptomyces albus KSM-35 were investigated The production of amylase was increased significantly by using sodium citrate and rice hull as a carbon source. In addition, the use of a mixture of sodium citrate and rice hull(1:1) resulted in increase of enzyme production by 20-fold when compared to that of soluble starch. The soybean meal as the nitrogen source could be partially replaced with yeast extract without changing the enzyme production yield. The amylase production was also increased by adjusting initial pH to 6.0 or by adding 0.01% SDS. Maximum amylase production was observed in the medium containing 1.5% sodium citrate, 1.5% rice hull, 0.7% soybean meal, 0.3% yeast extract, 0.66% K_2HPO_4, 0.05% MgSO_4·7H_2O, 0.08% CaCl_2·2H_2O, 0.01% SDS with initial pH of 6.0. The maximum yield of amylase reached 56.6 U/ml when B. subtilis LKS88(pASA 240) was cultured at 37℃ for 36 hr.

유방암의 뇌하수체 전이에 의한 양안 시력소실 1 예

최낙원,안명주,김재민,박동우,박용욱 한양대학교 의과대학 2000 한양의대 학술지 Vol.20 No.1

Pituitary gland metastasis is a rare clinical diagnosis, usually made in the setting of known primary systemic cancer with widespread metastasis to other organs. But visual loss as a presenting symptom of metastasis to the pituitary gland is unusal. We report here a case of 45-year-old woman who had been diagnosed as having metastatic breast cancer involving bone and liver 2 years ago, and received high dose chemotherapy with peripheral blood stem cell transplantation, developed sudden bilateral complete visual loss. At initial presentation, the patient had no light perception and no pupillary response to light in both eyes, but without evidence of panhypopituitarism, or diabetes insipidus. Brain MRI showed a large enhanced mass invasion to the cavernous sinus and extension to the suprasella portion without dura or base-of-skull metastasis. Pathologic examination showed metastatic breast cancer. Despite the transsphenoidal decompression surgery of metastatic tumor, the visual loss was not improved.

호텔기업의 로열티 프로그램이 몰입과 고객 애호도에 미치는 영향에 관한 연구

최우성,황명호 한국문화관광학회 2003 문화관광연구 Vol.5 No.2

In the hotel industry, the royalty (membership) program is very important because the profit or loss in their hotels income depends on it. The revenue from VIP membership takes 30~40% of total profits in the total hotel revenue. The utility value of the membership system is very powerful and hotels use this system to gain dominance over others. The hotels royalty program is a very important method in managing customers examining how to activate customer relationships, and analyzing how to affect customer loyalty. In the hotels royalty program, I researched how to influence level of commitment and loyalty.

전상환자에서 구순 구각부 결손에 대한 재건의 치험 4례

최규환,강명수,민복기,정철우 大韓顎顔面成形再建外科學會 1993 Maxillofacial Plastic Reconstructive Surgery Vol.15 No.3

A technical refinement for oral commissure reconstruction is presented. The oral commissure has an important role in oral sphincteric function. Once lost, the oral commissure is very difficult to restore and therefore, reconstruction of the oral commissure requires prevention of this function as well as prevention of microstomia. Trauma and tumor excisions are the most frequent reasons that will necessitate reconstruction of the oral commissure. Direct approximation of the wound margins after resection of the corrner of the mouth could easily induce microstmia and difficulty in opening. Thus case presented here deals with distortion of noraml mouth angle and acquired microstomia due to burn and tissue deface following shotgun and explosive wounds. The a mucosal flap approach was used to rehabilitate a natural looking mouth angle showing satisfactory results function and esthetic wise.

무선 ATM에서의 효율적인 자원할당 알고리즘

최명렬,조태경,권재우 漢陽大學校 工學技術硏究所 2000 工學技術論文集 Vol.9 No.1

본 논문에서는 셀을 섹터로 구분하고 이동국이 어느 섹터에 위치하는가를 추정하여 핸드오버할 확률이 가장 높은 최인접셀을 정하므로써 효율적인 대역폭 할당을 할 수 있는 핸드오버 알고리즘을 제안하였고, 서비스 트래픽을 실시간과 비실시간으로 구분하므로써 무선 멀티미디어 환경에 알맞은 대역폭 할당을 가능하게 했다. 또한 이에 적합한 무선 MAC프로토콜을 제안하여 구현하고자 하는 무선 ATM환경을 보다 구체적이며 실제적으로 나타냈다. 본 논문에서 제안한 알고리즘은 기존의 핸드오버 알고리즘과 SMPL로 비교 시뮬레이션하여 성능을 검증해 보았다. We propose the handover algorithm considering the position of mobile terminal and determining the most adjacent cell with the highest probability of handover. The proposed algorithm can make a effective bandwidth allocation and may be applicable to the condition of wireless multimedia. In addition, the proposed algorithm is considered efficient wireless MAC protocol. The simulation results of the proposed algorithm show better performance in both blocking probability and handover probability than conventional one.

운동분류에 따른 체구성 성분 및 BMI의 비교

최명화,권영우,김진해,민경선,민진아 한국스포츠리서치 2001 한국 스포츠 리서치 Vol.12 No.4

The purpose of the present was to investigate the body composition and BMI in different sports groups with classification of sports. 121 male collegiate athletes were recruited from varsity team of golf(n=10), field hokey(n=18), tennis(n=8), judo(n=17), weight lifting(n=27), boxing(n=19) and bicycle(n=22). Sports event can be classified as low static/low dynamic such as golf(LSLD), low static/high dynamic such as hokey and tennis(LSHD), high static/low dynamic such as judo and weight lifting(HSLD), high static/high dynamic(HSHD) such as boxing and bicycle. With classification of sports events, percentage of body fat was significantly higher with HSLD group compared to HSHD group, but no measurable difference was LSLD and LSHD groups. LBM was significantly greater with HSLD group compared to LSHD and HSHD groups. Furthermore BMI of the HSLD group was higher than the other groups(p<.05). In conclusion, high static/low dynamic group were greater in %fat, LBM, BMI than other groups. The differences observed between different sports groups and classification of sports are related to metabolic and dynamic properties in different sports events.

완전대혈관 전위의 심방내 교정술 후 추적 관찰

김연우,노정일,허준,윤명자,김호성,최정연,윤용수,양세원,이정렬,김용진,노준량 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.5

소양호 유역에서 비점오염원의 홍수유출과 오염수괴의 호수내 이동

허우명,김범철,김윤희,최광순 江原大學校 附設 環境硏究所 1998 環境硏究 Vol.15 No.-

In Korea most of nutrient tending into lakes is concentrated in stormy seasons. Phosphorus lording and transport of turbid storm runoff water was investigated in a reservoir, Lake Soyang, Korea. Concentration of phosphorus was measured at ten meter intervals on the monthly babe in dry seasons and biweekly in stormy seasons. The loading from drainage basin was determined by measuring total phosphorus (TP)concentration in the main in flowing stream, the Soyang River. Because total phosphorus concentration and flow rate of the Soyang River was high in flood period, most of annual phosphorus tending was concentrated in several sporadic storm runoff. In July of 181)8 phosphorus input was 418 tP/month. It was much higher than that in June, 6.0 tP/month. 10% of annual total phosphorus input was discharged through outlet during the period from ,lune to October. Turbidity was much higher(2~ 78 NTU) in stormy seasons than in dry seasons (1 ~ 9 NTU). Cold storm runoff laden with high turbidity and phosphorus flowed into metalimnion along the layer of same densify. Total phosphours concentration of intermediate turbid water ranged from 20 to 230 ?gp/m³. After stormy seasons high nutrient concentration was con-sistently observed at intermediate layer until autumn turnover. In autumn descending mixing depth caused increase of phosphorus concentration in the epilimnion.