대장내시경검사의 전처치로서 항콜린제 사용에 관한 연구 : 전향적, 이중맹검법으로

박경남,한동수,이민호,최호순,박준용,손주현,이오영,함준수,전용철,송승찬,기춘석,윤병철,이종희 대한소화기내시경학회 1997 Clinical Endoscopy Vol.17 No.3

Background: Use of antispasmodic medication prior to colonoscopy is controversial but someone believes antispasmodic may improve visualization of colonic mucosa and ease colonoscope insertion. So, we designed a study to assess the effect of premedication with the antispasmodic, hyoscine-N-butyl bromide(Buscopan') on the performance of colonoscopy. Methods: This study was prospective, double blinded, randomized, controlled study, One hundred three consecutive patients were randomized to receive intravenous buscopan lml(n=52) or placebo(n=51) combined with our standard initial medication(me- peridine 50 mg and midazolam 2 mg). Insertion of colonoscopy was timed, and 100 mm visual analogue scales (VAS) were used for asscssing difficulty of procedure, colonic motility, frequency of positional change, frequency of external compression, difficulty of assistance and degree of discomfort experienced by the patients. Results: There were no significant differences of intubation time between buscopan group(mean time, 7.23 min., range 2~15) and placebo group(7.07 min., range 3-25), (p=0.83) and withdrawal time between buscopan group (6.46 min., range 2-22) and placebo group(6.76 min., range 2 25), (p=0.69). Also, there was no significant differences in intubation time between males and females(buscopan; males 7.00 min., females 7.60 min., p=0.34, placebo; males 7.0~5 min., females 7.08 min., p 0.44). The VAS scores checked by endoscopist(p=0.29), assistant(p=0.32) and patient (p=0.15) were not significantly different in both groups. There were no significant differences in intubation time, VAS scores nf endoscopist, assistant, and patients. Conclusion: Premedication with intravenous bu.opan has no advantage on colonoscopy procedure. Use of antispasmodic medication prior to colonoscopy was not considered as recommendable agent.

Pulse Oximetry and Skin Temperature Gradient as Diagnostic Tools of Successful Caudal Block

Duk Kyung Kim,Kyoung Min Lee,Won Kyoung Kwon,Chung Sik Oh,Sung Whan Jang 대한마취과학회 2007 Korean Journal of Anesthesiology Vol.53 No.6

Background: Though caudal block is a relative simple technique, it has not been widely used in adults because of a high failure rate. We assumed that any tests to quantify the changes of sympathetic tone in the affected areas would be excellent indicators of successful block. We tested the usefulness of two candidates (pulse oximetry plethysmographic waveform amplitude measured at 5th toe and calf minus 5th toe skin temperature gradient) as indicators of successful caudal block. Methods: In 45 adult patients undergoing anal surgery with caudal block, these two variables were simultaneously measured at 2-min intervals for 20 min. A two-fold increase in the plethysmographic waveform amplitude from baseline and skin temperature gradient of 0°C were predefined as test criteria of successful block. Results: While the sensitivity, specificity, positive predictive value, and negative predictive value of the skin temperature gradient test were 45.9%, 100%, 100%, and 9.1%, those of the plethysmographic waveform test were 86.5%, 100%, 100%, and 28.6%. The plethysmographic waveform test showed a significantly higher discriminative capacity than the skin temperature gradient test (94.9% vs. 48.7%, P < 0.05) Conclusions: Unlike the skin temperature gradient test, the plethysmographic waveform test showed a considerably high validity in detecting successful block. Considering its simple and real time monitoring potentials together with a high failure rate of caudal block in adults, we cautiously recommend it as a supplemental diagnostic tool to predict successful block, especially when verbal communication with patient is difficult. (Korean J Anesthesiol 2007; 53: S 19∼25)

Studies on Lytic, Tailed Bacillus cereus-specific Phage for Use in a Ferromagnetoelastic Biosensor as a Novel Recognition Element

( In Young Choi ),( Joo Hyeon Park ),( Kyoung Min Gwak ),( Kwang-pyo Kim ),( Jun-hyun Oh ),( Mi-kyung Park ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.1

This study investigated the feasibility of the lytic, tailed Bacillus cereus-specific phage for use in a ferromagnetoelastic (FME) biosensor as a novel recognition element. The phage was immobilized at various concentrations through either direct adsorption or a combination of 11-mercapto-1-undecanoic acid (11-MUA) and [N-(3-dimethylaminopropyl)-N’-carbodiimide hydrochloride and N-hydroxysuccinimide (EDC/NHS)]. The effects of time and temperature on its lytic properties were investigated through the exposure of B. cereus (4 and 8 logCFU/ml) to the phage (8 logPFU/ml) for various incubation periods at 22°C and at various temperatures for 30 and 60 min. As the phage concentration increased, both immobilization methods also significantly increased the phage density (p < 0.05). SEM images confirmed that the phage density on the FME platform corresponded to the increased phage concentration. As the combination of 11-MUA and EDC/NHS enhanced the phage density and orientation by up to 4.3-fold, it was selected for use. When various incubation was conducted, no significant differences were observed in the survival rate of B. cereus within 30 min, which was in contrast to the significant decreases observed at 45 and 60 min (p < 0.05). In addition, temperature exerted no significant effects on the survival rate across the entire temperature range. This study demonstrated the feasibility of the lytic, tailed B. cereus-specific phage as a novel recognition element for use in an FME biosensor. Thus, the phage could be placed on the surface of foods for at least 30 min without any significant loss of B. cereus, as a result of the inherent lytic activity of the B. cereus-specific phage as a novel recognition element.

개의 췌도분리에서 췌장의 팽창과 콜라겐 분해요소

이상훈,김성주,박정환,김종성,오승훈,한진수,정인경,양태영,김동준,김광원,이문규,민용기,이명식,정재훈,함종렬 대한당뇨병학회 2000 Diabetes and Metabolism Journal Vol.24 No.2

Backgrounds: One of the main problems conditioning the outcome of islet transplantation is the ability to separate a sufficient number of viable islets with preserved function. Islet purification is critically affected by all of the isolation stages. Thus, it is necessary to set up the standard isolation method that islets are separate well from acinar without compromising islet yield and viability. Methods: Twenty three adult mongrel dogs were used for the experiment of total pancreatectomy with islet isolation. The islets were properly isolated by a modified Recordi method. The obtained islets were further purified by centrifugation on discontinuous gradients using cell separation system (Model 2991, Cobe, Lakewood Colo). We evaluated islet number (islet equivalent number, 150 um equivalents/kg of recipient body weight, IEq/kg), purity, cell volume, viabilty, recovery rate, and comparison of outcome according to the isolation conditions. Results: 1) The mean of islet numbers before purification were 13543±9431Eq/kg, digestion times were 13.8±2.6 min., digestion temperature was 37.4±0.2℃, purity was 59.7?.0%, viability was 90.0±2.1%, cell volume was 4.7±1.1 mL, islet number after purification were 4064±361 IEq/kg, and recovery rate was 29±2.9%. 2) Isolated islet numbers were different according to the degree of pancreas distension with collagenase, digestion temperature, and digestion time. 3) The best conditions for islet isolation were above 37.5℃ in temperature at recirculation of collagenase, within 12 min in digestion time and well distended pancreas with collagenase. 4) According to multiple regression adjusted by variable factors, the degree of pancreas distension with collagenase and digestion time were independently associated factors for successful islet isolation. Conclusions: In this study, we concluded that the degree of pancreas distension with collagenase and digestion time were independent factors for successful islet isolation and the best conditions for islet isolation were above 37.5℃ in temperature at recirculation of collagenase, within 12 min in digestion time and well distended pancreas with collagenase.

The Korean Association of lnternal Medicine : Poster Session ; PS 1357 : Nephrology ; Age is the Strongest Effector for the Relationship between Estimated Glomerular filtration Rate and Coronary Artery Calcifi cation in Apparently Healthy Korean Adults

( Hyun Beom Chae ),( Eun Jung Rhee ),( Hae Jung Park ),( Min Kyung Lee ),( Won Seon Jeon ),( Se Eun Park ),( Cheol Young Park ),( Won Young Lee ),( Ki Won Oh ),( Sung Woo Park ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Cardiovascular disease (CVD) is a major clinical problem and the most common cause of morbidity and mortality in patients with chronic kidney disease (CKD). Coronary artery calcifi cation (CAC) is a potential mechanism that explains the association between renal function and cardiovascular mortality. We aimed to evaluate the association between renal function and CAC in apparently healthy Korean subjects. Methods: In 23,617 participants in the health screening program in Kangbuk Samsung Hospital health screening program from January 2010 to December 2011, estimated glomerular fi ltration rate (eGFR) was assessed by Cockcroft-Gault equation. Coronary artery calcium score (CACS) was measured by multi-detector computed tomography (MDCT). Subjects were divided into 3 groups according to the CKD Staging system with eGFR grade; stage 1: eGFR = 90 mL/min/1.73 m2, stage 2: eGFR 60-89 mL/ min/1.73 m2, stage 3: eGFR 30-59 mL/min/1.73 m2. Results: Mean age of the participants was 41.4 years, and mean eGFR was 103.6±21.7 mL/min/1.73 m2. Hypertension and diabetes were noted in 43.7% and 5.5% of the participants, respectively. eGFR showed weakly negative but signifi cant association with CACS in bivariate correlation analysis (r=-0.076, p<0.01). Mean CACS signifi cantly increased from CKD stage 1 to 3. The proportion of subjects who had CAC signifi cantly increased from CKD stage 1 to 3. Although odds ratio for CAC signifi cantly increased from stage 1 to 3 after adjustment for confounding factors, this signifi cance was inversed when age was included in the model. Age was the strongest factor that affects CAC. Conclusions: This study performed in young Korean subjects with relatively normal renal function, age was the mostly strongest effector on the association between eGFR and the degree of CAC.

만화 과제를 이용한 정신분열병 환자의 사회인지 능력의 측정

오정은,나명현,하태현,신용욱,노규식,홍순범,이경진,신민섭,이영호,권준수 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.3

Objectives : This study is to investigate the hypothesis that schizophrenic patients have difficulty in inferring mental state of others. Methods : Twenty schizophrenic patients and twenty normal subjects were administered to the Cartoon Task required inferring the character's intention in a given context. Results : The schizophrenic patients showed a statistically significant impairment in the Cartoon Task. Both groups chose the card depicting a frequent everyday action significantly more rather than the similar card to the last picture in each story for wrong answers. Conclusion : These preliminary results suggest that schizophrenia is associated with a specific deficit of the cognitive ability referred to as social cognition, and this deficit can be detected not only in the acute phase as found in previous research studies, but also in remission. Also when subjects fail to appreciate what is going on in the minds of other people, they seem to rely on a socially familiar experience.

Flavobacterium odoratum의 TOL 플라스미드를 전달받은 광합성세균으로부터의 수소 생성

오순옥,조인선,이희경,민경희 숙명여자대학교 자연과학연구소 1991 자연과학논문집 Vol.- No.3

Flavobacterium odoratum SUB53의 TOL 플라스미드의 크기는 83 Md 이었으며 TOL 플라스미드에 의한 m-toluate의 최고분해농도는 5 mM이었다. Rhodopseudomonas sphaeroides KCTC1425의 수소생성은 nitrogenase의 활성과 비례하였으며 30 mM malate acid와 질소원으로서 7mM glutamate의 최적농도에서 최고의 수소생성을 보여 주었다. 한편, 0.3mM NH ion에 의해 nitrogenase의 활성이 저해되어 수소 생성이 현저히 감소하였다. F. odoratum SUB53과 Pseudomonas putida mt-2의 TOL 플라스미드는 R. sphaeroides에로의 접합시 수용세포의 대수증식기에서 10시간-15시간 동안에 최적이었으며 helper plasmid pRK2013에 의해 접합능이 증가하였다. Transconjugants인 R. sphaeroides C1(TOL SUB53)과 C2 (TOL mt-2)의 catechol-1,2-oxygenase(C-1,2-O)와 catechol-2,3-oxygenase(C-2,3-O)의 활성을 비교 검토한 결과 C-2,3-O 유전자를 code하는 유전인자는 TOL 플라스미드 위에 있으며, C-1,2-O 유전자의 위치는 chromosome 위에 있는 것으로 사료된다. 전자 공여체로서, m-Toluate는 R. sphaeroides C1과 C2의 TOL 플라스미드에 의하여 분해되어 수소를 생성함을 알 수 있었다. 이 결과는 TOL플라스미드에 의하여 m-toluate가 일차적으로 분해되고 생성된 이차산물로 부터 광선하에서 수소를 생성하는 것으로 추정된다. TOL plsmid size of Flavobacterium odoratum SUB53 was estimated as 83 Md and the optimum concentration of m-toluate degradation by TOL plasmid was 5 mM. H₂ production by Rhodopseudomonas sphaeroides KCTC1425 was largely dependent on nitrogenase activity and showed the highest at 30 mM malate with 7mM glutamate as nitrogen source. Nitrogenase activities were inhibited by 0.3mM NH ions, to be appeared the decrease of H₂ production. Conjugation of TOL plasmids from F. odoratum SUB53 and Pseudomonas putida mt-2 to R. sphaeroides showed the optimum at the exponential stage of recipient cells in presence of helper plasmid pRK2013. According to the investigation of catechol-1,2-oxygenase(C-1,2-O) and catechol-2,3-oxygenase (C-2,3-O) activities of R. sphaeroides C1 (TOL SUB53) and C2 (TOL mt-2), the gene for C-2,3-O is located on TOL plasmid and gene for C-1,2-O on the chromosome of R. sphaeroides. m-Toluate was biodegraded by TOL plasmid in R. sphaeroides C1 and C2, presumably to be produced H₂ gas from the secondary metabolites of m-toluate.

당뇨병-고지혈증 모델동물의 개발

오승현,노경진,박인선,민본홍,두호경,안세영,김용석,성제경 한국환경독성학회 2004 환경독성보건학회지 Vol.19 No.3

Diabetic complication is one of major risk factors leading to vascular disease Such as atherosclerosis stroke, coronary heart disease and etc Several factors affecting the acceleration of diabetic vascular complication have been known such as hypertension hyperlipidemia, immune complex and genetic factors To screen and develop new therapeutics agents for diabetic vascular complication, it is strongly needed to develop animal models for diabetic complications However in rodents models, diabetic complications is not well developed Furthermore to asses￠ the possibility of new therapeutics for diabetic vascular complications, diabetic animal models which have the risk factors of diabetic complications is needed We aim to develop and establish an diabetic animal model which have diabetic complications with hyperlipidemia which is one of risk factors for diabetic complications We induced insulin-dependent diabetes by intra venous injection of streptozotocin (35 mg/kg/day) in RICO rats which is a spontaneous animal model for hyperlipidemia Our models (STZ RICO) showed hyperglycemia, persistent high level of plasma cholesterol and triglyceridemia with severe diabetic renal changes until 28 weeks after induction of diabetes STZ-RICO rats could be used for the evaluations of newly developed diabetic drugs.

버퍼층 및 열처리 효과가 CoFe/Cu 다층박막의 자기저항에 미치는 영향

오미영,송은영,이장로,김희중,김경민,김미양 숙명여자대학교 자연과학연구소 1996 자연과학논문집 Vol.- No.7

DC magnetron sputtering방법에 의해 Corning glass기판 위에 ?? 형태로 다층박막을 제작하여 버퍼층 종류(Fe, Cu, Cr, Ta)와 두께, 비자성층인 Cu두께 변화에 관한 자기저항비의존성을 조사하였다. 또한 이들 시료에 대하여 열처리를 행한 후 열처리가 이 시료의 구조, 자기적 성질 및 자기저항에 미치는 영향을 조사하였다. Fe버퍼층의 두께가 60Å일 때 극대 자기저항비가 14%인 자기저항이 관찰되었고 Fe버퍼층과 Cr버퍼층을 갖는 시료를 비교한 경우 자기저항비는 그다지 차이가 없으나 Fe버퍼층이 있는 경우에 더 큰 포화자기장 및 자기 이력현상을 나타내었다. 250℃까지의 시료에 대한 열처리는 다층박막의 주기성을 유지한채 더 큰 결정립을 갖게 하여 자기저항비는 증가하였으나 그 이상의 온도에서는 계면 혼합 및 계면 확산에 의한 감소를 나타내었다. Giant magnetoresistance (GMR) and magnetic properties of CoFe/Cu multilayers prepared by dcmagnetron sputtering has been studied. We investigated the effect of Fe buffer layer thickness, different buffer layers, and annealing condition on GMR. For the 60Å Fe buffer layer thickness, maximum MR ratio 14% was found. When we have used different buffer layers, multilayers with Cr and Fe buffer show similar GMR magnitude, but multilayers with an Fe buffer have much higher saturation fields and hysteresis. The study of the dependence of the MR behaviors on annealing temperature, the MR ratio was increased to 250℃, but reduced at the temperature higher than 300℃ because of the interfacial diffuse.

비장경색이 동반된 쯔쯔가무시병 1예

오종찬,이범주,장재현,원경준,박경희,김동민,박치영,정춘해 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.1

Tsutsugamushi disease is an acute febrile illness caused by Orientie. tsutsugsmushi. It is characterized by fever, myalgia, lymphadenopathy, and rash. And it can be easily diagnosed by characteristic eschar and serologic testing. Nearly all of the patients with tsutsugamushi disease improve with antibiotics such as doxycycline. However, the fatality rate of untreated cases is seven to ten percent. Splenic infarction is not common disease which is caused by occlusion of the main splenic artery or any of its branches. It is mainly caused by emboli that arise from cardiovascular disease, but sometimes by regional thrombosis associated with leukemia, myeloproliferative disorder, sickle cell anemia, pancreatitis, splenic torsion and septicemia. We experienced one case of tsutsugamushi disease with splenic infarction, which was improved after doxycycline treatment finished.