방선균 분리주가 생산하는 Phospholipase C 저해물질인 MT2617-2B의 분리 및 특성

고학룡,이현선,오원근,안순철,김보연,강대욱,민태익,안종석 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

방선균 분리주 MT2617-2의 배양액으로 부터 phospholipase C (PLC) 저해물질인 MT2617-2B를 n-butanol 추출 및 column chromatography 법을 이용하여 분리하였다. MT2617-2B는 IR ^13C- 및 ^1H-NMR 그리고 ESI-MS에 의한 구조분석 결과, 한 개의 hemiketal ring, polyhydroxyl 및polymethyl groups으로 구성되었으며 side chain으로 한 개씩의 malonate 및 guanidine group을 가지는 분자량 1057의 macrolide 화합물이었다. 따라서, MT2617-2B를 기존의 macrolide 항생제인 copiamycin 및 niphithricin A로 동정하였다. 한편, MT2617-2B는 methanol 용액에서 실온에서 방치하였을 때 도일한 분자량을 가진 두 개의 이성질체를 생성하였다. PLC γ1과 -β1에 대해 각각 25 및 50㎍/㎖의 IC_50 값을 가지며, Staphylococcus aureus 와 Candida albicans에 대해서는 항균활성을 나타내지만 Escherichia coli에는 나타내지 않았다. A phospholipase C (PLC) inhibitor (MT2617-2B) was isolated from the culture broth of actionmycetes isolate MT2617-2 by the extraction with n-butanol and column chromatographic techniques. The molecular weight of the inhibitor was 1057, by the spectroscopic analyses of IR ^13C- and ^1H-NMR and ESI-MS. The chemical structure of MT2617-2B was found to be a macrolide compound consisted of a hemiketal ring, polyhydroxyl and polymethyl groups, which had a malonate and guanidine group as its side chain. MT2617-2B produced its two isomers having the same molecular weight by standing in methanol solution at room temperature. Therefore, MT2617-2B was identified as copiamycin and niphithricin A, macrolide antibiotics. The values of IC_50 against PLC γ1 and PLC-β1 were 25 and 50㎍/㎖, respectively. MT2617-2B had antimicrobial activities against Staphylococcus aureus and Candida albicans, but not against Escherichia coli.

하드웨어 設計環境에서의 CDFG 形態에 관한 硏究

金賢培,金忠錫,朴淳東,高炳吾 新羅大學校 1991 論文集 Vol.32 No.1

In this study, design CDFG format in hardware design environment. It is used for high level synthesis in hardware design environment and supported in VHDL(VHSIC(Very High Speed Integrated Circuit) Hardware Description Language) Design Environment. This CDFG supports most of characteristic in hardware description language, concurrency, sequence, hierarchy etc. It is designed in consideration of VHDL, C, PASCAL, HardwareC. First, design CDFG and describe description rule then represent example of translation and grammar for CDFG paser.

물질남용자의 유형화에 대한 예비적 연구

김현수,조선미,이용석,임기영,이호영 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.1

목 적: 본 연구는 물질남용자들의 인구학적 특성, 물질남용 특성, 인성특성을 파악하는 것을 통해 물질남용자들의 유형화를 하기 위하여 예비적으로 시행된 것이다. 방 법: 본 연구자가 수행하는 집단프로그램에 참여한 사람들을 직접 면접하고, MMPI, BDI, 이화방어기제검사 및 충동성검사 등을 시행하고 이에 대한 빈도분석과 Chi-square 검증 그리고 문항에 따라 F-검증을 실시하였다. 결 과: 1) 본 집단은 대마가 14명, 필로폰이 26명, 유해화학이 2명이었으며, 1년 이상 사용한자가 27명(64.35%) 이었고, 사회경제적으로 중이하가 많았으며 20-30대가 32명(76.5%)이었다. 학력 및 경제적 상태는 다양했으며, 직업도 다양했다. 전과는 15명이었다. 2) 발달력 상 부모관계가 나쁘거나 상실하거나, 이혼, 분리 등의 경험이 많으며, 상관분석상 전과는 비행력과 비행력은 부모약물, 부모관계, 전과와 유의미한 상관이 있었다(p<0.05). 3) MMPI 군집상 두 집단으로 분류가 되었으며 한 집단은 단순약물사용군으로, 다른 집단은 사회병질집단으로 명명하였다. 두 집단과 발달력과의 상관에서 사회병질집단은 부모관계, 부모약물과 통제적으로 유의미했다(p<0.05). 4) 물질종류에 따른 MMPI 척도에서 Mf, Si 척도가 유의미하게 상승했으며, 부모상실, 부모이혼, 부모학대가 있는 집단은 MMPI 척도에서 Pd 척도가 유의미하게 상승했다(p<0.05). 결 론: 이상의 결과로 볼 때 발달력, 물질종류에 따른 유형화의 가능성이 향후 더 연구되어져야 할 것으로 생각이 되며 이를 치료프로그램과 연결하는 연구가 앞으로 수행되어져야 할 것이다. Objectives: This preliminary study was performed to typify a group of substance abusers by their demographic data, developmental history, substance use patterns, and personality profiles. Methods: Forty-two substance abusers were assessed with Minnessota Multiphasic Personality Inventory(MMPI), Ehwa-Defense Mechanism Test, Beck Depression Inventory(BDI), Beck Anxiety Inventory(BAI), etc. They also participated in semi-structured interview by psychiatrists and social workers. Results: 1) This group consisted of 14 cannabis users, 26 methamphetamine users, and 2 inhalant users. Twenty-seven(64.35%) of this group abused drugs over one year. Thirty-two(76.5%) of the subjects were in their twenties or thirties. Many of them were less than low in their economic status. Their academic careers and occupations were various. Fifteen abusers had past criminal histories. 2) Among their developmental history, parental alcohol problem, poor relationship with parent, and criminal history were significantly correlated with one another(p<.05). 3) In the cluster analysis upon their MMPI profiles, two clusters emerged; one cluster presented low profiles, and the other showed high profiles. We named the former simple drug user group, and the latter `sociopathic drug abuser group`(p<.05). 4) There differences in MMPI profiles according to the kind of drugs and developmental problems. Methamphetamine group was higher in Mf and Si. The group with poor developmental history was higher in Pd profiles(p<.05). Conclusion: These results suggest that, for typology of substance abusers, more investigation should be made on developmental history and drug use patterns. This study presented two clusters of simple abuse group and sociopathic abuse group.

거주 형태에 따른 에너지 섭취량과 소비량의 균형도 조사

박순목,고성경,남인수,윤재만,임승현,전중기,이종삼 대구대학교 인문과학연구소 2008 人文科學硏究 Vol.31 No.-

We investigated that the effects of residential type on energy balance in college students. Total sixteen college students were participated in this study, all subjects were assigned one of three groups: either school attendee students group, self-governed living students group, dormitory students group. Routine physical activity level (for 5 days including three weekday and two weekend) and food intake were surveyed. For investigation of degree of physical activity, all subjects were requested to record on their physical movements as possible as detail should be obtained. To all subjects, five-day dietary log form was given, and used for examining of calorie intake from their routine diet. There was no statistical difference in energy intake and consumption in each. However energy consumption was significantly higher than energy uptake in school attendee students group. All other groups were shown a similar energy values between energy uptake and consumption. There were no significant differences in energy intake and consumption in any of experimental groups when comparisons were made between weekdays and weekend. As far as energy uptake was concerned it was no statistical difference in any of major nutrients among groups. In conclusion, partial imbalance was found between energy intake and uptake in school attendee groups. This may be due to their more active life style than other groups'. In future studies, better controlled study should be performed not only more subjects are recruited but also minor nutrients are included for examining of energy balance.

인공 석회화 기질의 개발 및 파골세포에 의한 기질흡수에 관한 연구

김현만,박주철,임도선,박수진,김윤지,고재승 대한구강해부학회 1997 대한구강해부학회지 Vol.21 No.1

To develop artificial matrix resorbable by osteoclasts can be used as a model system that biological function of osteoclasts would be assayed in vitro in a standardized condition instead of using heterogenous bone or dentin slices. The present study developed artificial substrates consisted of apatite crystals. collagen gen. agar, apatite crystals under collagen film, mixture of apatite crystals and collagen, and mixture of agar and apatite crystals. And osteoclastic attachement and resorption activity on the each substrate was assayed in vitro. Osteoclasts attached to apatite crystals, collagen, mixture of apatite crystals and collagen, and mixture of apatite crystals and agar, however, not to agar. This suggested that apatite crystals and collagen should be exposed on the surface of artificial substrate where osteoclasts can attach. Osteoclasts attached to the substrate resorbed the substrate consisted of apatite crystals, mixture of apatite crysals and collagen, and apatite crystals under thin film of collagen, however, not of collagen, mixture of apatite crystals and agar. This suggested that the first phase of osteoclasts in resorbing matrix is to secrete acid and the end result of this phase, whatever it may be, may activate secretion of hydrolytic enzymes specific to organic components.

척추손상 쥐의 지방유래 줄기세포와 미노사이클린의 운동기능 회복효과

장재혁,고현윤,차영선,박형욱,신용범,손현주 부산대학교 병원 암연구소 2009 부산대병원학술지 Vol.- No.25

한우, 칡소 및 제주 흑우 Calpain-Calpastatin 유전자 다양성

이승환,김승창,조수현,최봉환,Aditi Sharma,임다정,당창권,장선식,김재환,고문석,양보석,강희설 충남대학교 농업과학연구소 2013 농업과학연구 Vol.40 No.2

The aim of study was to investigate genetic diversity for the calpain/calpastatin gene in three Hanwoo breeds [(Brown (n=62), Brindle (n=81) and Jeju Black (n=30)]. Random samples from three breeds of Hanwoo were selected and genotyped for the 7 SNPs of calpain/calpastatin using TaqMan method. Allele frequencies were investigated for CAPN1/CAST gene. Allele frequency of CAST2 SNP was 0.75, 0.59 and 0.22 for Brown, Brindle and Jeju black, respectively. The CAST3 revealed allele frequency of 0.59 and 0.57 in Brown and Jeju Black, while it showed very low allele frequency (0.07) in Brindle. In particular, favorable allele (G allele) for the CAPN1-2 SNP which was shown a strong association with tenderness in Taurine and Indicine cattle revealed 16% and 17% higher allele frequency in Brown Hanwoo (0.82) comparing Brindle (0.66) and Jeju Black Hanwoo (0.65). AMOVA demonstrated that among population variance occupied only 10% of total variance and among individual variance was 0%, while within individual variance was 90% of total variance. This result showed that population effect contributed very small portion of genetic to these three Hanwoo breeds, while within individual variance contributed large portion of genetic diversity within these Hanwoo breeds. In conclusion, three Hanwoo breeds (Brown, Brindle and Jeju black) showed a genetically homogeneous based on the 7 SNPs of CAPN1/CAST gene and it came from same ancestor to form modern Hanwoo breed.

신이식 환자에서 발생한 Nocardia elegans 폐농양 1예

박기호,고선영,오련,김탁,조오현,김양수,우준희,한덕종,성흥섭,김미나 대한감염학회 2008 감염과 화학요법 Vol.40 No.2

노카르디아는 면역 저하 환자에서 발생하는 기회 감염균으로 배양과 균 동정이 복잡하기 때문에 시기 적절한 진단과 치료가 어려울 때가 흔하다. 그러나 최근 16S 리보좀 RNA 염기서열 분석과 같은 새로운 분자생물학적 방법이 도입되면서 노카르디아 감염의 진단과 치료에 많은 도움이 되고 있다. 저자들은 신이식을 받은 39세 남자에서 발생한 N. eleeans에 의한 폐농양을 경험하였기에 보고하는 바이다. 환자는 항균제 감수성 결과에 따라 적절한 항균제로 치료하였고, 치료 종료 후 1년 동안 재발 없이 외래 경과 관찰 중이다. 본 증례를 통하여 이식 환자와 같은 면역 저하 환자에서 노카르디아 감염의 가능성을 고려하여야 하겠고 분자생물학적 방법에 의한 빠른 균 동정과 적절한 항균제 치료가 중요함을 기억하여야 하겠다. Nocardiosis is well-known opportunistic infection in immunocompromised patients. The timely diagnosis and treatment, however, is stilt a difficult problem. Recently, as newer molecular techniques, particularly 16S rRNA sequencing, are implemented, the diagnosis and management of the infection are improving. We report a case of lung abscess in a 39-year-o1d-man who underwent kidney transplantation. He was diagnosed of Nocardia elegans by 16S rRNA sequencing and was cured of the infection with appropriate antibiotic therapy.

한의진단명과 진단요건의 표준화 연구 II (표준화 실례) : 2차년도 연구결과 중간 보고

양기상,최선미,최승훈,안규석,박경모,박종현,김성우,신승호,정우열,전병훈,고현,김정범,신상우,김성훈,김동희,권영규,엄현섭,장혜옥 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the second year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows; - differential diagnosis according to condition of body fluid, differentiation of syndromes according to the state of qi and blood, differential diagnosis according to relative excessiveness or deficiency of yin and yang(氣血陰陽津液辨證) - differentiation of diseases according to pathological changes of the viscera and their interrelation - analyzing and differentiating of febrile diseases in accordance with the theory of the six channels(傷寒辨證) The individual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name(異名), notion of diagnosis pattern, index of differentiation of symptoms and signs(辨證指標), the main point of diagnosis, analysis of diagnosis pattern(證候分析), discrimination of diagnosis pattern(證候鑑別), a way of curing a diseases(治法), prescription(處方), herb in common use(常用藥物), diseases appearing the diagnosis pattern(常見疾病), documents(文獻調査). This study was carried out on the basis of the Chinese documents and references.

저칼슘식이로 사육한 생쥐의 골수세포 배양에 의한 파골세포양세포의 형성

박주철,강선주,임도선,김현만,고재승 대한구강해부학회 1995 대한구강해부학회지 Vol.19 No.1

Although it is now well established that osteoclasts are derived from mononuclear hematopoietic stem cells, most likely within the granulocyte-macrophage lineage, the exact differentiation process of osteoclast precusors has only been partially discovered. This study was carried out to investigate the following topics by examining the osteoclast-like cell formation from the calcium-deficient mouse bone marrow cells; 1) the effect of calcium deficiency on the formation of the mononuclear phagocytes and the mononuclear precusors of the osteoclast in the mouse bone marrow; 2) the effect of TNF (tumor necrosis factor)-α on the differentiation of osteoclast-like cells from the calcium-deficient mouse bone marrow culture; 3) the formation of the osteoclast-like cell from the calcium-deficient mouse bone marrow mononuclear cells and the relationship between the osteoblast cell line(MC3T3E1) and osteoclast-like cell formation; 4) formation of the osteoclast-like cells from the mononuclear cells which were col1ected from PTH-pretreated mouse bone marrow cells to investigate the relationship between PTH and calcium deficiency in osteoclast-like cell formation. Bone marrow cells were isolated from marrow of mouse feeding normal diet or calcium deficient diet for 7 and 14 days. Bone marrow mononuclear cells were fractionated by cetrifugation on Hypaque-Ficoli density gradients. PTH-pretreated mouse bone marrow mononuclear cells were obtained by collecting the nonadherent cells after the culture of normal mouse marrow cells with PTH for 6 days. The prepared cells were cultured on dentin disc at the concentration of 1.5-2 X 10^(6) cells per 0.5ml of a-MEM containing 10% FCS for 3, 5 and 7 days. Thereafter, TRAP staining, NSE staining, effect of calcitonin on the osteoclast-like cell and scanning electron microscope analysis of resorption lacunae on the dentin disc were performed. The results were as follows; 1. Number of the osteoclast precusors and mononuclear phagocytes in bone marrow was increased by calcium-deficient diet. 2. More osteoclast-like cells were formed from the calcium-deficient mouse bone marrow cells than from normal cells. 3. Formation of osteoclast-like cells was significantly stimulated by TNF-α in normal mouse bone marrow cells and calcium-deficient mouse bone marrow cells at concentration of l0ng/ml and 100ng/ml. 4. Osteoblast cell line(MC3T3E1) did not play an important role in osteoclast-like cell formation. 5. Formation of the osteoclast-like cells from the mononuclear cells which were collected from PTH-pretreated mouse bone marrow cells was similar to that of calcium-deficient mouse bone marrow mononuclear cells, which suggests that the effect of calcium deficiency in osteoclast formation may be mediated by PTH.