구강해부학에 관한 Bartolomeo Eustachio의 업적: “Libellus de Dentibus”의 주요 내용

이영훈,박병건 대한구강해부학회 2018 대한구강해부학회지 Vol.39 No.1

우리에게는 귀인두관(Eustachian tube)을 명명한 해부학자로 잘 알려진 Bartolomeo Eustachio가 치아의 치수와 치주인대에 대해서 처음 언급한 것을 포함해서 구강해부학에 관해 매우 중요한 기여를 하였다는사실이 최근에 널리 알려짐에 따라, 그를 ‘구강해부학의 아버지(Father of Dental Anatomy)’라고 부르자는주장이 제기되었다. Eustachio는 치아에 관한 최초의 단행본 책자인 “Libellus de Dentibus”를 1563년에 발간하였다. 그 동안 이 책은 여러 언어로 소개되기 시작했는데, 본 논문에서는 Chernin과 Shaklar가 1999년에 발간한 라틴어·영문 대조판 책(A Little Treatise On The Teeth)과 2000년에 이것에 대해 요약 발표한 논문을 주로 활용하여 “Libellus de Dentibus”의 주요 내용을 소개함으로써, 구강해부학에 관한 Bartolomeo Eustachio의 위대한 업적을 다시 한번 기리고자 한다.

구강조직 미세환경 특이적 구강청량제 개발의 필요성

김태영,정재광,안서영,하정홍,김안나,이상일,안창현,김지연,김재영 대한구강해부학회 2023 대한구강해부학회지 Vol.44 No.1

Recently, oral care market is mainly driven by increasing people’s awareness about oral hygiene. Mouthwashes are becoming increasingly popular among oral care products. Consumers in oral care market desire for new or more functionally improved mouthwashes. To develop and commercialize new conceptual and functional mouthwash products that are tissue- and condition-specific, including gingivitis, periodontitis, and implantitis, it is necessary to understand and consider the relevant cellular and histological research results findings accumulated by advanced research in science and technology. In particular, periodontal disease, which is the most common oral disease, can be prevented and treated depending on the progress and oral tissue specific micro-environment. Therefore, it is necessary to analyze the limitations of existing mouthwashes and establish a foundation for development new products with remarkable ingredients. 전세계적으로 위생 및 개인 외모 유지에 대한 의식이 높아지면서 구강 위생 시장이 확대되고 있다. 특히구강청량제는 휴대하기 간편하고 입안을 헹궈내는 방식으로 사용이 가능하기 때문에 사용하는 소비자가 늘어나고 있다. 이에 따라 관련 제품의 시장 규모도 지속적으로 성장하고 있으며 프리미엄 콘셉트 및 고기능성구강용품의 개발에 대한 소비자들의 관심이 더욱 높아지고 있다. 이러한 시장환경을 선도할 수 있는 신개념의 구강위생제품의 개발 및 상용화를 위해서는, 고도화된 과학기술에 의해 축적된 연구결과를 이용한 제품의 개발 및 생산이 절실히 요구되고 있다. 특히 외래 다빈도 상병 진료 1위를 차지하는 치주질환은 진행정도와 구강환경에 따른 예방과 치료가 가능하여, 기존 구강청량제의 한계에 대해 분석하고 기능적으로 월등하고 시너지를 기대할 수 있는 기능성 성분들을 탑재하여 새로운 제품을 생산할 수 있는 기반의 확립이 필요하다.

손상된 얼굴신경의 재생에 관한 고찰

박문성,박영석,이승표,백기석,장미숙 대한구강해부학회 2012 대한구강해부학회지 Vol.33 No.1

The fac ial nerve can be damaged by trauma. infection and surgery Injury to this nerve results in multiple dysfunction of muscles of face and ocular adnexa that have cosmetic. functional and psychological impacts on the patients. Although the peripheral nerve system has an ability to regeneration. recovery of nerve is dependent on multiple factors. Despite of advances in surgical technique and a good rate of structural nerve healing. functional recovery of injured nerve remains incomplete. For functional recovery of injured nerve. innovative strat egies which enhance the ability of regeneration are published. In this review. after a brief description of the anatomy and factors contributing to nerve regeneration. we examine the therapy of injured nerve and then survey the recently published strategies . Injured nerve can be treated by surgical methods such as anastomosis. nerve grafting and neural conduits. Surgical interventions can be used for the treatment of severed nerve. Timing of surgery is controversial But recently. it is generally accepted that primary surgery has many advantages . For the functional recovery of nerve. the growth factor and neurotrophic factor can be applied with surgical therapy. The growth factor are Nerve growth factor and Brain-derived growth factor that are released by Schwann cell. For peripheral nerve regeneration. ultrasound. microwave. and LASER have been used to increase regeneration ability and to promote the functional recovery. These physiological therapy induced the thermal effect. the activation of Schwann cell. and the improvement of functional recovery. Phamaco1ogic intervention for nerve regeneration is proposed. The pharmaco1ogic approaches focused on the modu1ation of the Schwann cell that is a crucia1 ro1e of nerve regeneration. These agents are neurotransmitters and neurohormones. Recent1y. gene therapy and use of stem cell for improving regeneration are proposed. Gene therapy is the delivery of therapeutic gene which trans1ated the growth factor or neurotrophic factor to the injured site. The studies on new intervention of using the stem cell which differentiate into Schwann cell-1ike cell is in progress.

The effect of magnetic fields on the MC3T3-E1 cells attachment

이희수 대한구강해부학회 2012 대한구강해부학회지 Vol.33 No.1

To determine the effects of magnetic fields by comparing interaction between the osteoblast and titanium dental implant with a magnet which is inserted in the specially designed implant abutment. Dental implants connected with a magnet - inserted abutment were used. A cell-spreading assay in which percentages of cells at four different stages of attachment were identified by scanning electron microscopy and quantified within 30 min. 4hour and 24hour attachment periods. The surfaces of dental implant connected with a MagScrew showed significantly enhanced rates of cell spreading in comparison with the others. Based on these observations. it appears reasonable to suggest that magnetic fields promote the differentiation and maturation of osteoblasts by affecting the expression of adhesion proteins imbedded in the cell membrane and may have a favorable effect on immediateloading implant treatment by reducing the processing time for mineralization.

사람치수줄기세포 조건배지와 법랑모세포 조건 배지가사람치수줄기세포의 상아모세포 분화에 미치는 영향

최원준,박주철 대한구강해부학회 2012 대한구강해부학회지 Vol.33 No.1

The differentiation ability of denta1 pu1p stem cells(DPSCs) is clinically very usefu1 for the regeneration of dentin-pu1p comp1ex. It is important to confirm that dentin-pu1p comp1ex is made when DPSCs differentiate with conditioned media(CM) inc1uding the signa1 mo1ecu1es such as various enzymes. growth factors , hormones and so on. We can identify the odontogenic differentiation of DPSCs using an odontogenic marker such as dentin sia1ophosphoprotein(DSPP) mRNA. Because the ro1e of odontob1asts is very important to deve10p dentin-pu1p comp1ex. This research is going to confirm whether treatments of condi tioned media (CM) facili tate odontob1asts differentiation of human DPSCs in vitro. When human denta1 pu1p stem cell CM (hDPSC-CM) , ame1ob1ast CM(AM-CM) , the mixture of AM-CM and hDPSC-CM are treated to hDPSCs. we examine the capacity of odontogenic differentiation of hDPSCs. Human DPSCs was exp1anted from human third mo1ars . We cu1tured to differentiate hDPSCs with hDPSC-CM. AM-CM. the mixture of hDPSC-CM and AM-CM. Human DPSCs cu1tured without CM were contro1. They were divided into 4 groups. We differentiated hDPSCs for 0, 3, 7, 10 days and operated rea1 time PCR using DSPP mRNA promoter as an odontogenic marker and BSP mRNA promoter as an osteogenic marker. Expression of DSPP mRNA on differentiation of hDPSCs with the mixture of hDPSC-CM and AM-CM increased than control after 3. 10 days and increased than on differentia tion of hDPSCs with hDPSC-CM. AM-CM respectively. Expression of BSP mRNA increased than control after 3 days but decreased after 7. 10 days. Dentin formation is important meaning on pulp regen eration . When pulp exposes stimuli . pulp develops reparative dentin. which means a sort of regeneration . Conclusively. the mixture of hDPSC-CM and AM-CM improves the ability of odontogenic differ entiation of hDPSCs and helpful to regenerate pulp.

The study of essential factors that affect mandibular dental arch forms by cluster analysis

Michiko Nakatsuka,Chizuko Inui-Yamamoto,주성숙,김지연,박영석,Shunji Kumabe,Katsura Ueda,Chun-Ying An,Aiko Morishita,신제원,Yasutomo Iwai 대한구강해부학회 2013 대한구강해부학회지 Vol.34 No.1

To elucidate dent al arch morphological characters in young Japanese adolescents. we studied morphological and dimensional characteristics of the dental arch forms in 396 sets of dental stone study models (age: 18-26: ma le: 257. female: 139) . 53 mandibular arches (male: 29. female: 24) in the diagnostic casts of normal dentition and occ1usion were selected and evaluated. We determined midpoints of the incisal ridge of the incisors (Ih. Ih. 12R & 12d. cusp tips of the canines (CR & Cd. summits of buccal cusps of the premolars (Ph. Ph. P2R & P2d. summits of mesiobuccal cusps of the molars (Mh. Mh. M2R & M2d and the midpoint A of line (Ih-Ih) . Point B was defined as the intersection of the vertical line from point A with the line (M2R-M2L). Point E was the intersection of (A-B) with (CR-Cd. The midpoint of line (M2R-M2L : original line) is defined as point O. We examined the mandibular arch forms by estimation of length. angles and the transition of anterior and buccal segments which were represented by items developed from the reference points and lines . The obtained data were studied using a hierarchical c1uster analytic approach: the factor loading of principal components in the c1usters O.e.. pt’ 2nd and 3rd c1usters) were further evaluated By the analyses. we observed significant differences in each c1uster.especially of the arch length. protrusion of canines. degree of curvature of anterior teeth segment and the degree of roundness of the mandibular dental arches. Arch length of the 3rd cluster was longer than that of the other clusters . In addition. we noticed that width differences of the dental arch and anterior segment were useful to distingui sh between the 1st and 2nd clusters . The study concludes that the mandibular dental arch form consisted of essential components of arch length. arch width and the curvature of anterior teeth. Besides. we can effectively divide the mandibular arch form into clusters by dental arch length and width.

하치조 신경 손상 및 회복에 대한 고찰

백창현,이승표,장미숙,박영석 대한구강해부학회 2012 대한구강해부학회지 Vol.33 No.1

Inferior a1veo1ar nerve (IAN) is the branch of mandibu1ar division of trigemina1 nerve and is one of the most important nerves in oromaxillary area. In many clinica1 situations. there is the possibi1ity of IAN being injured. Thus, the anatomic properties of IAN were studied first. We a1so studied the reasons of IAN l nJ Ury, the diagnostic methods which he1p determine the extent of injury and the methods for treatment of injured nerve. We reviewed the bio1ogica1 changes of injured IAN. IAN cou1d be damaged from 10wer third mo1ar extraction or imp1ant surgery. When IAN is injured, all the parts of IAN including cell body, axon , Schwann cell and nerve ending wou1d be changed for the nerve regeneration. Schwann cells seem to have a very important ro1e in this process. There are severa1 methods for eva1uating the extent of IAN injury: static 1ight touch test , dynamic 1ight touch test , two point discrimination test. therma1 sense test. e1ectrophysio1ogic test and digita1 infrared therma1 imaging. Injured IAN can be treated by pharmaco1ogica1 therapies. physica1 therapies. and surgica1 interventions such as anastomosis and nerve graft. Recently. the studies on new intervention of using nerve conduits or Schwann cells in IAN repair are on progress.

삼차신경의 감각신경 손상에 관한 진단, 치료, 재생에 관한 고찰

이선영,박영석,이승표,백기석,장미숙 대한구강해부학회 2012 대한구강해부학회지 Vol.33 No.1

The trigemina1 nerve is the fifth crania1 nerve which are consist of ophtha1mic nerve. maxillary nerve. and mandibu1ar nerve branch. The trigemina1 nerve functions both as the chief nerve of sensation for the face and the motor nerve controlling the muscles of mastication. Prob1ems with the sensory part of the trigemina1 nerve resu1t in pain or 10ss of sensation in the face. Recent1y. the 1l1jUry of trigemina1 nerve including sensory part is increased by trauma. surgica1 procedure 1ike imp1ant. patho1ogic edema. But. nerve 1l1jury show more limited regeneration and repair reaction than any other tissue like skin. bone. vessel. therefore it is necessary that proper diagnosis. repair therapy and basic science research for cellu1ar. genetic regeneration process are converged in clinica1 treatment fie1d. The visua1 ana10gue sca1e and McGill pain questionaire are most common diagnosis method for sensory testing of sensory nerve injury. Depending on the severity of nerve 1l1jUry. it is sometimes impossib1e nerve to recover their function normally by themse1ves. Surgica1 intervention like nerve repair. decompression. nerve graft permit anatomic reconstruction and successfu1 reinnervation. The interruption of the continuity of an axon. such as may occur with nerve 1l1jury or as a resu1t of inflammation. edema. or compression. 1eads to characteristiv cahnges in the carious segments of the neuronal unit. The cellu1ar 1eve1 and mo1ecu1ar 1eve1 change follow axonal injury. microg1ia1 proliferation and activation . reactive astrocyte. synaptic stripping. and functiona1 activation of the 10ca1 vascu1ature are important components of this non neurona1 cellu1ar response. And proinflammatory cytokines such as IL6. regeneration-associated genes (RAGs). Mye1in- associated inhabitors(MAIs) a1so p1ay centra1 ro1e in successfu1 regeneration. Convergence of proper diagnosis. repair intervention and basic science research for regeneration procedure are applied for improve prognosis and qua1ity of life significant1y.

Cytosine Arabinoside와 Polyribonucleotides가 흰쥐 切齒齒髓組織에 미치는 影向에 關한 硏究

金成龍 대한구강해부학회 1977 대한구강해부학회지 Vol.1 No.1

Male rats, weighing about 100gm were used throughout all experiments. TheY were divided to cytosine arabinoside group, cytosine arabinoside group receiving polyribonucleotides and control group. Experimental animals were given an intramuscular injection of cytosine, arabinoside 50mg per kg of body weight. Polyadenylic acid (poly A) and polyuridylic acid (poly U), were used in this investigation. Experimental rats, were given an intraperitoneal injection of poly A:U(lmg/kg) , 24 hours prior to sacrifice. The animals were sacrificed 1, 3, 5, 7, 10 and 14 days after injection of these drugs. For determination of DNA content, the diplienylamine, reaction of Burton was used. Total protein of the pulp tissues were measured by the Folin phenol reagent. The intensity of the subsequent color reaction was determined by absorbance at 600mμ for DNA and 750mμ for protein in the spectronic 20 spectrometer. The results were compared with data derived from calf thymus DNA and bovine serum albumin as standard and expressed as μg of DNA and protein. To establish the relative cellularity of the pulp tissues from different groups, D A and protein content per mg of tissue were assessed. The objectives of this study were to evaluate the effects of cytosine arabinoside group and cytosine arabinoside group receiving poly A: U on the biochemical functioning of pulp tissues in rat incisors. The results were as follows; 1. The effects of cytosine arabinoside group and cytosine arabinoside group receiving polyribonucleotides on the body weight and weight of pulp tissues were produced significant decrease during the first 5th days followed by a gradual recovery thereafter. The body weight and pulp tissue weight of the cytosine arabinoside group receiving polyribonucleotides were higher than that not receiving polyribonucleotides. 2. The DNA and protein content in the groups receiving polyribonucleotides were higher than those not injected with nucleotide mixture. 3. The effects of cytosine arabinoside group without and with poly A:U on t he DNA and protein content of the pulp tissues were produced a considerable decrease during the first 5th days followed by gradual recovery thereafter. 4. Therefore, the results suggest that poly A:U might have a limited stimulatory effect on the DNA and protein synthesis of the pulp tissues in rat incisors. It has been shown that synthetic, polyribonucleotides, when used in an appropriate quantity were able to enhance DNA and protein synthesis in the pulp tissues of the rat incisors.

대한구강해부학회를 포함한 몇몇 치의학단체 표상(表象)들에 관한 소고(小考)

박병건 대한구강해부학회 2012 대한구강해부학회지 Vol.33 No.1