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이중현,김용섭,이정호,이창우,김상욱,이문섭,오민구,하달봉,이경섭 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-
신장이식은 환자의 삶의 질을 높이고 거의 정상적인 사회생활을 가능하게 함으로써 말기신부전 환자의 3가지 신대체 요법(혈액투석, 복막투석, 신장이식) 중에 가장 효과적이고 각광받는 치료법이라 할 수 있다. 최근 들어 의료기술과 의학의 발달로 신장이식의 성공률은 과거에 비해 비약적으로 높아지고 있으며 국내에서도 점차 신장이식수술을 받는 환자가 증가하고 있으나 서울이나 다른 대도시에 비해 의료환경이 열악한 중소도시나 지방의 병원에서는 아직도 시행하지 못하고 있는 형편이다. 이에 저자들은 경북 최초로 자체 의료진에 의해 고혈압과 말기 신부전으로 동국대 포항병원에서 혈액투석을 받고 있던 32세의 남자환자에게 부인으로부터 신장을 제공받아 신장이식을 성공적으로 시행하였기에 문헌고찰과 함께 보고하고자 한다. Kidney transplantation is the preferred treatment modality for many patients with end-stage renal disease (ESRD), because it offers improved quality of life over both hemodialysis and peritoneal dialysis. Patients who do well after transplantation generally report improvement in vitality and freedom to return to the style of life that they experienced before their progression to ESRD. Recently, the success rate of kidney transplantation is greatly being increased as the surgical technique and immunosuppressive drugs are developed. Kidney transplantation is also popular in Korea which has good result. But, until now the general hospitals which are located at small city can't try the kidney transplantation because they don't have proper facilities and experts. We firstly experienced a case of kidney transplantation in a 32 years old man with hypertension and chronic renal failure at KyoungBuk province. So we report this case with references.
건축공사시방서에서의 KS규격의 국제인증방안에 관한 연구
김경식,이재섭,김용수,이규진,조용훈,최완철,전재열,이현수,구재동,이기환 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1
The purpose of this research is make out construction specifications for International Construction. Using off-shore products incur cost increase and longer delivery term of construction. and inconvenient on International Construction. Expand the range of additional local products conforming to performance. Life Cycle Cost. maintainability. operability. user's acceptability, etc.. And this study is suggest the standard formats and the techniques for construction specification.
( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.
판상형 충전제의 함량과 배향에 따른 PP복합체의 열팽창계수 영향 연구
이기윤(Kee Yoon Lee),이용현(Yong Hyun Lee),정선경(Sun Kyoung Jeoung),황효연(Hyo Yeon Hwang),이승구(Seung Goo Lee) 한국고분자학회 2012 폴리머 Vol.36 No.3
두 가지의 종횡비(ρα=a1/a3 and ρβ=a1/a2)에 의해 특징지어진 3차원 타원체(a1 > a2 > a3)를 사용하여 polypropylene 복합체의 판상형 충전제 함량과 방향이 열팽창률에 미치는 영향에 대해 실험적인 연구를 수행하였다. 측정된 실험적인 값은 Lee와 그의 연구자들이 제안한 이론적인 모델에 의한 계산 값과 비교분석되었다. 판상형충전제로는 운모와 탈크가 사용되었다. 실험 결과로 운모의 경우 종횡비는 ρα= 13.5, ρβ= 1.8이 사용되 어, 20 wt% 함량일때 α11/αm는 약 0.56으로 감소하였으나, α33/αm는 오히려 1.018로 증가함을 보였다. 탈크의 경우의 종횡비는 ρα=3.7, ρβ= 1.4이었고 α11/αm는 0.63으로 감소하였다. 결국 운모와 탈크 모두 그 함량증가에 따라 종단방향과 횡단방향에서 열팽창률은 감소하였으나 수직방향에서는 초기 낮은 충전제 함량에서는 오히려 증가하는 경향을 보였다. Experimental study was performed regarding the effects of disc-like filler orientation and contents on the coefficient of thermal expansion (CTE) of polypropylene composites using the three dimensional ellipsoids (a1 > a2 > a3) analyzed by two aspect ratios(ρα=a1/a3 and ρβ=a1/a2). Measured data were compared with the theoretical approaches proposed by Lee et al. Mica and talc were useed as disk-like fillers in the composites. As experimental results, α11/αm decreased down to ca. 0.56 with mica content of 20 wt% and the aspect ratios, ρα=13.5, ρβ=1.8. However, α33/αm increased to more than 1. In the case of talc, α11/αm decreased to ca. 0.63 with 20 wt% and ρα=3.7, ρβ=1.4. Finally, the longitudinal CTEs (α11) of polypropylene composites decreased as filler contents increased, but normal CTE (α33) increased in the low filler contents like the theory.
Plant regeneration from callus of Iris odaesanensis Y. N. Lee native to Korea via organogenesis
Bae, Kee-Hwa,Yoo, Kyoung-Hwa,Lee, Mi-Hyun,Jeong, Jae-Hun,Choi, Yong-Eui,Yoon, Eui-Soo The Korean Society of Plant Biotechnology 2013 식물생명공학회지 Vol.40 No.3
Iris odaesanensis Y. N. Lee. is an important endangered and native plant belonging to the family Iridaceae in Korea. This study describes a method for rapid micropropagation of this species via from leaf, rhizome and root explants derived calli. Leaf, rhizome and root explants were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) for callus induction. Rhizome explants yielded calli at a frequency of 72% when cultured at 1.0 mg/l 2,4-D. Calli were maintained at 1.0 mg/l 2,4-D. These calli were transferred to MS medium supplemented with 0, 0.5, 1.0, and 2.0 mg/l 2,4-D in combination with 0, 0.5, 1.0, and 3.0 mg/l BA for adventitious shoot induction. The highest number of adventitious shoot (228.9 per petri-dish) were formed at 1.0 mg/l 2,4-D and 1.0 mg/l BA. WPM medium was the best to convert calli into plantlets, where up to 98.2% of calli were regenerated into plantlets. This in vitro propagation protocol should be useful for conservation of this endangered plant.
판상형 충전제의 함량과 배향에 따른 PP복합체의 영률 변화 연구
서상범(Sang Bum Seo),이용현(Yong Hyun Lee),정선경(Sun Kyoung Jeong),이승구(Seung Goo Lee),이기윤(Kee Yoon Lee) 한국고분자학회 2012 폴리머 Vol.36 No.2
이 논문은 판상형 충전제의 함량과 배향에 따라 폴리프로필렌 복합체의 영률 변화에 미치는 영향을 연구 하였다. 이론적인 예측은 Lee와 그의 연구자들에 의해 제안된 두 개의 종횡비, ρα=a1/a3과 ρβ=a1/a2 를 이용하여 3차원 타원체의 형상을 계산하였고 실험값과 비교 분석하였다. 판상형 충전제로 운모와 탈크를 사용하였다. 충전제의 형태는 SEM을 이용해 관찰하였고, 종횡비는 고분자의 흐름을 기준으로 좌표축을 설정하여 통계적 방법으로 계 산하였다. 충전제가 운모인 경우에 ρα=13.5와 ρβ=1.8이었고, 탈크의 경우는 ρα=3.8과 ρβ=1.4이었다. 또한 충전제 의 2개의 종횡비와 함량이 기계적 물성에 미치는 영향을 실험적으로 연구하였다. 운모의 경우 30 wt% 함량일 때 E11이 약 2.7배 향상되었다. 탈크의 경우는 E11이 약 2.3배 향상하였다. This paper studied the effects of the content and the orientation of the disk shape fillers on the modulus of PP composites. The experimental results were compared with the theoretical calculations which included the three dimensional ellipsoids and analyzed by two aspect ratios, ρα=a1/a3 and ρβ=a1/a2 proposed by Lee and his researchers. Mica and talc were used as disk shape fillers in the composites. The shapes of mica and talc were observed by SEM and aspect ratios were statistically calculated. For the case of mica, the average aspect ratios were ρα=13.5 and ρβ=1.8, and for the case of talc, they were ρα=3.8 and ρβ=1.4. Also, the effects of two aspect ratios and the content of filler on the mechanical properties were studied: For 30 wt% of mica, E11 increased up to about 2.7 times, and for the other case of talc, E11 increased up to about 2.3 times, respectively.
화상을 입은 백서에서 식이의 성분이 세균전위에 미친 영향
이광만,이경근,조지현 圓光大學校 醫科學硏究所 1997 圓光醫科學 Vol.13 No.1-2
Bacterial translocation means the passage of viable bacteria from the gastrointestinal tract to normally sterile tissues such as the mesenteric lymph nodes and other internal organs. It is now clear that translocation is increased in bums and in injury including hemorrhagic shock. Total parenteral nutrition and elemental enteral diets are thought to be associated with atrophy of the intestinal mucosa and increased translocation. The purpose of this study was to determine whether or not the composition of nutrients effects on bacterial translocation in normal and burned rats. 54 male Sprague-Dawley rats were randomized to 3 groups of 18 animals each. Group Ⅰ was non-burned control rats, and group Ⅱ and Ⅲ were burned rats. Rats in group Ⅱ and Ⅲ were subjected to contact dermal bum of 10% and 20% each of their body surface area. All 3 groups were re-randomized to 3 subgroups of 6 animals each; subgroup 1 fed rat chow, subgroup 2 fed elemental diet(Energen®) and subgroup 3 fed oral TPN solution. Animals were fed respective diets ad libitum for 2 weeks, and then mesenteric lymph nodes, portal vein blood, and cecal content were aseptically obtained for culture. Bacterial count in mesenteric lymph nodes(MLN) of burned rats was significantly increased than that of non-burned rats(p=0.033). In burned rats, MLN bacterial count was the highest in the rats fed oral TPN (566.1±870.5/g vs. 270.1±382.4/g in the rats fed elemental diet and 164.9±184.7/g in the rats fed chow). Cecal culture in burned rats revealed a statistically significant increase in the cecal anaerobic count in the rats fed oral TPN when compared to the rats fed chow or elemental diet(p=0.003). There was close correlation between MLN bacterial count and cecal aerobes count(r=0.843, p=0.001). In conclusion, the type of nutrients had influence on bacterial translocation in burned animals partly by increasing the cecal bacterial population.