콘택트렌즈 보존 용기 유래 Acanthamoeba lugdunensis KA/L5주의 내공생세균

정동일,공현희,김태호,황미열,유학선,윤호철,설성용 경북대학교 의학연구소 1999 경북대학교병원의학연구소논문집 Vol.3 No.1

콘택트렌즈 보존 용기 유래 가시아메바 KA/L5주의 세포질 내에 존재하는 bacterial endosymbiont(내공생세균)를 투과전자현미경으로 관찰하여 확인하였다. 숙주인 가시아메바 KA/L5주는 형태학적으로 제2군에 속하였고, rDNA PCR-RFLP 결과 A. lugdunensis로 동정되었다. 미토콘드리아 DNA RFLP와 동위효소 분석상 이 충주는 국내 콘택트렌즈 보존용기에서 가장흔히 분리되는 type인 KA/Ll주, 국내 임상 분리주 중 하나인 KA/E2주, 내공생세균을 가지는 것으로 보고된 병원 냉각수 유래 KA/W4주 및 L3a주와 동일하거나 매우 유사한 성적을 보였다. 내공생세균은 약 1.38× 0.507㎛의 크기였고, 아메바 세포질 내에 불규칙하게 분포하고 있었으며. 그 표면에 아메바의 ribosome이 부착되어 있었다. 내공생세균을 둘러싼 lacunae나 막과 같은 구조는 관찰되지 않았다. Legionella 특이 primer를 이용한 효소중합반응(PCR)에서 내공생세균의 염색체 DNA는 증폭되지 않았다. A. lugdunensis의 우리말 이름을 담수가시아메바로 제안한다. Transmission electron microscopy of an Acanthamieba isolate (KA/L5) from a contact lens case revealed bacterial endosymbionts within cytoplasm of the amoebae, The Acanthamoeba isolate belonged to the morphological group II. Based on the polymerase chain reaction (PCR) - restriction fragment length Polymorphism (RFLP) of 18S ribosomal RNA coding DNA (rDNA), the Isolate was identified as A. lugdunensis. Strain typing by isoenzyme analysis using isoelectric focusing (IEF) and mitochondrial (Mt) DNA RFLP revealed that the isolate was closely related with KA/Ll , the most predominant type of isolates from contact lens storage cases, KA/E2, a clinical isolate, KA/W4, preciously reported to host endosymbionts, and L3a strains of A. lugdunensis. The endosymbionts were similar to those of KA/W4 In aspects that they were randomly distributed in both trophozoites and cysts, and were rod-shaped bacteria measuring approximately 1.38x 0.50㎛. But the number of endosymbionts per amoeba was significantly lower than that of KA/W4. They were neither limited by phagosomal membranes nor included In lacunae- like structure.

전격성 경과를 취한 만성 호산구성 폐렴 환자 1예

윤호상,진춘조,유광하,이상엽,이수인,정상만,김선두,이순제,이길도,전혜정 건국대학교 의과학연구소 2000 건국의과학학술지 Vol.10 No.-

Chronic eosinophilic pneumonia(CEP) is a rare disease of unknown etiology characterized by chronic infiltration of the lung with eosinophils. It presents with profound systemic symptoms comprising fever, night sweats, weight loss, dyspnea and blood eosinophilia with nonsegmental air-space consolidation confined to the outer third of the lung, the "photographic negative of pulmonary edema". Histopathologic features of chronic eosinophilic pneumonia are intraalveolar and interstitial infiltrations with eosinophils, histiocytes, giant cells, scattered lymphocytes and plasma cells. Glucocorticoid therapy cause prompt resolution of symptoms as well as disappearans of blood eosinophils, elevated serum Ig E levels and the roentgenographic lesions. Infrequent radiographic findings include nodular infiltrates, consolidations, cavitations, atelectasis and pleural effusions. Deaths from CEP although rare, have been reported, but the majorities of CEP have benign courses and do not need a therapy with ventilator. We report a case of chronic eosinophilic pneumonia, which had a sudden course associated with diffuse pneumonic consolidations on the both lung and bilateral pleural effusion.

다발성 골수종으로 이행한 단일형질세포종 1예

정재용,모성환,기장도,문철호,김창욱,전용준,박유환,정춘해,오윤경 조선의대 1995 The Medical Journal of Chosun University Vol.20 No.2

벼 품종의 芳香性 유전

金光鎬,鄭允惠 건국대학교 농업자원개발연구소 2000 農資源開發論集 Vol.22 No.-

Segregation mode of leaf aroma in F2 and F3 were studied in ten crosses between the three scented rices and five non-scented rice varieties. Three crosses among ten showed the segregation ratio of 1 scented : 3 non-scented plants in F2, two crosses indicated 3 : 13, and four cross fitted to both 1 : 3 and 3 : 13. Segregation ratio of F3 populations of Ilpumbyeo/Hyangmi and Jinbuchalbyeo/Hyangmi indicated that aroma of Hyangmi was not controlled by the single recessive gene, but a few recessive genes. The aroma and heading date in F2 of seven crosses did not segregate independently. The frequency of scented plants was lower in delayed heading plants than that of earlier heading plants.

Real-time PCR 기술의 생물학적 폐수처리에서의 응용

엄호섭,이선미,상병인,정윤철 한국물환경학회 2006 한국물환경학회지 Vol.22 No.2

골아세포 MC₃T₃-E₁의 증식과 기능에 Insulin Like Growth Factor Ⅰ, Ⅱ 및 부갑상선 호르몬이 미치는 영향

윤현구,정호연,양인명,김성운,김진우,김광원,김영설,최영길,서광식 대한내과학회 1992 대한내과학회지 Vol.42 No.1

MC₃T₃-E₁골아세포군을 이용하여 IGF-Ⅰ과 Ⅱ를 투여하고 DNA합성과 골아세포기능을 측정하기 위해 PTH에 대한 C-AMP반응정도, alkaline phosphatase 활성도를 측정하여 다음과 같은 결과를 얻었다. 1) IGF-Ⅰ과 Ⅱ는 모두 DNA합성이 어느 농도까지는 증식효과를 나타내지만 그 이후에는 억제되는 양상을 보였다. 2) PTH에 대한 골아세포의 C-AMP반응으로 증가되었지만 IGF-Ⅰ과 Ⅱ의 투여시 농도변화에 따른 의미있는 변화는 없었다. 3) 본 실험의 조건에서 alkaline phosphatase 활성도는 증가되지 않았다. 이상의 결과로 MC₃T₃-E₁ 골아세포에 IGF-Ⅰ과 Ⅱ는 세포의 증식을 자극하여 PTH는 C-AMP생성을 촉진하는 효과가 있었다. The effect of insulin-like growth factor (IGF) Ⅰ and Ⅱ on cloned MC₃T₃-E₁ cells that had osteoblastic activity was examined by [³H] thymidine incorporation into DNA, cyclic AMP responsiveness to parathyroid hormone (PTH), and alkaline phosphatase activity. IGF Ⅰ and Ⅱ stimulated the growth and proliferation of osteoblastic cells at 20ng/㎖ and 50ng/㎖, respectively. We next examined the effect of IGF-Ⅰ and Ⅱ on the expression of osteoblastic activity. Adenylate cyclase response of PTH to IGF-Ⅰ and Ⅱ-treated cells showed maximal stimulation at 10 and 50㎚PTH, respectively, but IGF Ⅰ and Ⅱ induced limited activity of osteoblastic function. In conclusion, IGF-Ⅰ and Ⅱ were potent stimulators to the growth of osteoblastic cells but did not have an immediate effect on expression of osteoblastic function in this experiment.

濟州道公務員의 特性과 庶政刷新에 관한 硏究

申宗淳,鄭允武,朴瑾浩,金武昌 동국대학교 행정대학원 1977 행정논집 Vol.7 No.-

플라즈마 산화 처리된 65/35황동의 산소 분압별 부식특성에 관한 연구

박창규,안승호,김정구,정윤모,한전건 대한금속재료학회 2005 대한금속·재료학회지 Vol.43 No.4

Brasses are the most widely used copper-based alloys because of the good corrosion resistance, formability, high strength and excellent hot-working properties. However, dezincification occurs when one or more components of an alloy are more susceptible to corrosion than the rest and, as a result, are preferentially dissolved. In order to enhance the corrosion resistance, the oxide film was deposited on the 65/35 brass by pulsed dc plasma oxidation with various pressure. The corrosion behavior of plasma oxidized brasses with various oxygen pressure was investigated by electrochemical methods (potentiodynamic polarization test, potentiostatic test, electrochemical impedance spectroscopy) and surface analyses (x-ray diffraction, scanning electron microscopy, auger electron spectroscopy, electron probe micro analyzer). Particular attention was paid to the effects of oxygen pressure and oxide thickness on the coating properties related to the corrosion behavior. From the results of electrochemical test, it was found that the oxidized brass under condition of 1.5 Torr oxygen pressure was effective in improving corrosion resistance. It was attributed to the protective oxidized layer (ZnO). Finally, an optimized oxidation condition for corrosion protection was found to be 1.5 Torr oxygen pressure and 1 pun thickness. (Received September 3, 2004)

핵연료에서 핵분열 생성물의 방출 기구에 관한 연구

양재춘,이호연,정문규 충남대학교 기초과학연구소 1986 연구논문집 Vol.6 No.-

중성자와 우라늄의 핵 반응에 의해 생성된 핵분열 생성물의 물리적 특성을 이용하여 원자로 내의 핵연료 상태를 해석하는 새로운 모델을 도입하였다. 고체 핵연료 내에서 핵분열 생성물의 생성과 이것의 원자로 냉각재까지 방출되는 과정을 계산하는 방정식을 세웠다. 이 방정식으로 핵분열 생성물의 source term을 계산하고, 이것이 고체 핵연료 내에서 방출되어 gap과 핵연료 피복재를 통하여 원자로 냉각재까지 방출하는 양을 계산하였으며, 원자로 1차 냉각재에서의 tramp uranium양을 계산하였다. 이 방정식을 computer로 계산하기 위하여 전산화 하였다. 실험 자료로는 가압 수형 원자로 (Pressurized Water Reactor)인 고리 원자력 발전소 1호기의 제 3주기 동안에 핵연료의 상태를 감시하기 위해 1차 냉각재를 분석해서 얻은 핵분열 생성물의 방사능 강도를 이용하였다. 이 실험 자료와 위 방정식에서 계산한 방사능 강도를 비교하여 결함 핵연료 수와 tramp uranium 양을 구하였다. 이렇게 구한 결과와 고리 1호기의 제 3주기 말에 핵연료 재장진을 위해 수행한 sipping test 결과를 비교해 보았다. 그 결과 본 연구에서 도입한 모델이 잘 맞는 것을 알 수 있었다. We propose the new model which analyzes the nuclear fuel state of the reactor from the physical characteristics of nuclear fission products which are produced by reactions between neutron and uranium 235. The main feature of this model is to calculate the generation of fission products inside the solid nuclear fuel and the release mechanism of fission products into the reactor coolant. Using the computer we solved the equation of the model and put the experimental activity data of the fission products to determine the number of failed fuel rods and amount of tramp uranium. These data had been taken from the primary coolant of the Kori Nuclear Generating Station, Unit 1. This result of our calculation is in a fairly good agreement with the result of the sipping test during the refueling period for cycle 3 of the Kori Nuclear Generating Station, Unit 1. As a result of this research we conclude that our model for the release mechanism of unclear fission products in reactor fuel is well describing the actual processes.

새로운 Ca^2+ 길항제의 혈압강하효과

함원훈,임태균,정윤성,정윤호,양재권,최원철 성균관대학교 약학연구소 1993 成均藥硏論文集 Vol.5 No.1

Diltiazem and nifedipine, have been used throughout the world as an effective antianginal and antihypertensive agent. In order to increase the activity of these drugs, we synthesized the diltiazem-nifedipine coupling compounds. These compounds^(1-8) were tested for antihypertensive effect. Unfortunately, these compounds didn't show any promising activities.