연속수증기증류추출법에 의한 오렌지와 자몽의 휘발성 유기화합물 확인

홍영신,김경수 한국식품저장유통학회 2016 한국식품저장유통학회지 Vol.23 No.1

The volatile flavor components of the fruit pulp and peel of orange (Citrus sinensis) and grapefruit (Citrus paradisi) were extracted by simultaneous distillation-extraction (SDE) using a solvent mixture of n-pentane and diethyl ether (1:1, v/v) and analyzed by gas chromatography-mass spectrometry (GC-MS). The total volatile flavor contents in the pulp and peel of orange were 120.55 and 4,510.81 mg/kg, respectively, while those in the pulp and peel of grapefruit were 195.60 and 4,223.68 mg/kg, respectively. The monoterpene limonene was identified as the major voltile flavor compound in both orange and grapefruit, exhibiting contents of 65.32 and 3,008.10 mg/kg in the pulp and peel of orange, respectively, and 105.00 and 1,870.24 mg/kg in the pulp and peel of grapefruit, respectively. Limonene, sabinene, α-pinene, β-myrcene, linalool, (Z)-limonene oxide, and (E)-limonene oxide were the main volatile flavor components of both orange and grapefruit. The distinctive component of orange was valencene, while grapefruit contained (E)-caryophyllene and nootkatone. δ-3-Carene, α-terpinolene, borneol, citronellyl acetate, piperitone, and β-copaene were detected in orange but not in grapefruit. Conversely, grapefruit contained β-pinene, α-terpinyl acetate, bicyclogermacrene, nootkatol, β-cubebene, and sesquisabinene, while orange did not. Phenylacetaldehyde, camphor, limona ketone and (Z)-caryophyllene were identified in the pulp of both fruits, while α-thujene, citronellal, citronellol, α-sinensal, γ-muurolene and germacrene D were detected in the peel of both fresh fruit samples.

제주감귤류의 휘발성 향기성분의 확인

홍영신,김경수 한국식품저장유통학회 2016 한국식품저장유통학회지 Vol.23 No.7

The volatile flavor compounds in five Jeju citrus fruit varieties (Cheonhyehyang, Hallabong, Jinjihyang, Hwanggeum hyang and Redhyang) were extracted by simultaneous distillation-extraction (SDE) using solvent mixture of n-pentane and diethyl ether (1:1, v/v) and analyzed by using gas chromatography-mass spectrometry (GC-MS). The number of aroma compounds were identified with : 104 (4,939.77 mg/kg) in Cheonhyehyang, 98 (3,286.38 mg/kg) in Hallabong, 105 (3,317.56 mg/kg) in Jinjihyang, 102 (4,293.39 mg/kg) in Hwanggeumhyang, and 108 (4,049.94 mg/kg) in Redhyang. The detected main volatile compounds were; limonene, sabinene, β-myrcene, α-pinene, β-pinene, linalool, 4-terpineol, α-terpineol, (E)-β-ocimene and γ-terpinene. Among the identified volatiles compounds, ethyl-benzene, nonanol, 1-p-menthen-9-al, (E)-isocarveol, methyl salicylate, α-terpinen-7-al, perilla alcohol, and ethyl-dodecanoate were detected in Cheonhyehyang. only Furthermore, β-chamigrene and α-selinene were in Hallabong only; 3-hydroxybutanal, (E)-2-nonenal, isoborneol, octyl acetate, (E)-2-undecenal, β-ylangene and guaia-6,9-diene in Jinjihyang. ρ-Cymenene, β-thujone, selina-4,11-diene and (E,E)-2,6-farnesol in Hwanggeumhyang only; and ρ-cymen-8-ol, bornyl acetate, carvacrol, bicycloelemene, α-cubebene and 7-epi-α-selinene in Redhyang only. This study confirmed the differences in composition and content of volatile aroma components in five varieties of Jeju citrus fruits.

전자선 조사된 육포에서 생성된 hydrocarbon 류 분석

홍영신,이순임,전동복,김재성,정인선,최성화,이가현,노은영,최지연,김경수 한국식품영양과학회 2011 한국식품영양과학회 학술대회발표집 Vol.2011 No.10

Portable Soil pH Sensor Using ISFET Electrode

홍영신,정선옥,박종원,홍영기 한국정보통신학회 2022 Journal of information and communication convergen Vol.20 No.1

Fertilizers have long been used to increase crop yields; however, farmers are still having difficulties in managing fertilizers for growing crops as well as economic problems. The conventional method of soil sampling and laboratory analysis to determine soil pH is time consuming and costly; therefore, a portable pH sensor is developed to characterize spatial or temporal variability within fields via rapid and dense data acquisition. The portable pH sensor comprises an electrode unit, a portable console, and a USB connector. The soil water content (SWC) and electrical conductivity (EC) affect the electrical resistance of soil. An artificial test soil is performed to evaluate the effect of SWC and EC on soil pH. The test results show that stable pH measurements are achieved at SWCs greater than 20 mL (16.3%). Regardless of the SWC, the electric potential difference (EPD) remains at 2.5 g of NaCl. As the EC increases in the soil samples, the EPD increases.

유자, 금귤, 레몬 및 라임의 휘발성 향기성분의 비교

홍영신,이임식,김경수,Hong, Young Shin,Lee, Ym Shik,Kim, Kyong Su 한국식품저장유통학회 2017 한국식품저장유통학회지 Vol.24 No.3

본 연구는 한국에서 재배된 유자와 금귤을 수입되는 레몬 및 라임과 비교하였을 때 정유성분의 유용적 가치를 확인하고자 하였다. 유자, 금귤, 레몬 및 라임의 휘발성 향기성분 분석을 위하여 재증류한 n-pentane/diethylether(1:1, v/v) 혼합용매 100 mL를 사용하여 SDE 방법으로 3시간동안 휘발성 화합물을 추출한 후 GC/MS로 확인하였다. 유자, 금귤, 레몬 및 라임에서는 각각 104종(3,713.02 mg/kg), 87종(621.71 mg/kg), 103종(3,024.69 mg/kg) 및 106종(2,209.16 mg/kg)의 향기성분이 동정되었다. 4종의 Citrus속 과일의 주요 휘발성 향기성분은 limonene으로 확인되었으며, peak area는 각각 유자 35.03%, 금귤 63.82%, 레몬 40.35% 및 라임 25.06%로 나타났다. Limonene 이외에 유자의 주요 휘발성 향기성분은 ${\gamma}$-terpinene, linalool, ${\beta}$-myrcene, (E)-${\beta}$-farnesene, ${\alpha}$-pinene 및 ${\beta}$-pinene 등이며, 금귤에서는 ${\beta}$-myrcene, ${\alpha}$-pinene, (Z)-limonene oxide, (E)-limonene oxide, geranyl acetate 및 limonen-10-yl acetate 등 순으로 동정되었다. 레몬의 주요 향기성분은 ${\gamma}$-terpinene, ${\beta}$-pinene, ${\beta}$-myrcene, geranyl acetate, neryl acetate 및 (Z)-${\beta}$-bisabolene 등 순이며, 라임은 ${\gamma}$-terpinene, ${\beta}$-pinene, (Z)-${\beta}$-bisabolene, neral, geranial 및 neryl acetate 등 순으로 나타났다. 본 연구 결과 4종류 과일의 특징적인 향기성분이 동정되었으며, 휘발성 향기성분 조성비의 차이를 확인할 수 있었다. 또한 국내산 유자 및 금귤은 향료산업에서 널리 사용되는 레몬과 라임의 대체제로 충분한 활용가치가 있다고 사료된다. This study was conducted to confirm the usefulness of essential oil components in yuzu and kumquat cultivated in Korea for comparison with those in lemon and lime. The volatile flavor compounds in citrus fruits (yuzu, kumquat, lemon and lime) were extracted for 3 h with 100 mL redistilled n-pentane/diethylether (1:1, v/v) mixture, using a simultaneous steam distillation and extraction apparatus (SDE). The volatile flavor compositions of the samples were analyzed by gas chromatography-mass spectrometry (GC-MS). The aroma compounds analyzed were 104 (3,713.02 mg/kg) in yuzu, 87 (621.71 mg/kg) in kumquat 103 (3,024.69 mg/kg) in lemon and 106 (2,209.16 mg/kg) in lime. Limonene was a major volatile flavor compound in four citrus fruits. The peak area of limonene was 35.03% in yuzu, 63.82% in kumquat, 40.35% in lemon, and 25.06% in lime. In addition to limonene, the major volatile flavor compounds were ${\gamma}$-terpinene, linalool, ${\beta}$-myrcene, (E)-${\beta}$-farnesene, ${\alpha}$-pinene and ${\beta}$-pinene in yuzu, and ${\beta}$-myrcene, ${\alpha}$-pinene, (Z)-limonene oxide, (E)-limonene oxide, geranyl acetate and limonen-10-yl acetate in kumquat. Furthermore, ${\gamma}$-terpinene, ${\beta}$-pinene, ${\beta}$-myrcene, geranyl acetate, neryl acetate and (Z)-${\beta}$-bisabolene in lemon and ${\gamma}$-terpinene, ${\beta}$-pinene, (Z)-${\beta}$-bisabolene, neral, geranial and neryl acetate in lime were also detected. As a result, it was confirmed that the composition of volatile flavor compounds in four citrus fruits was different. Also, yuzu and kumquat are judged to be worthy of use alternatives for lemon and lime widely used in the fragrance industry.

고온기 송풍 덕트 적용 수직·수경재배 참외의 엽령별 광합성과 착과 절위별 과실 특성

홍영신,박소현,윤성욱,권진경,이시영,이상규,문종필,장재경,배효준,황정수 (사) 한국생물환경조절학회 2023 생물환경조절학회지 Vol.32 No.2

This study was conducted to apply with an air duct for the cooling and a utilizing cultivating method that uses the fruiting node and the defoliation to the high-temperature vertical and hydroponic cultivation of the oriental melon. The lower fruiting node (LF) was to remove all third vines generated from 5 nodes of a secondary vine. The higher fruiting node (HF) was fruiting on the third vine generated from a first node of the third vine. The direction of the stem string; upward (UW), downward (DW). Four treatment conditions were applied with the LF-UW, LF-DW, HF-UW (control), and HF-DW. The leaf age of melon leaves was measured for photosynthesis at 3 days intervals, and the fruit characteristic was conducted on 79 fruits in each treatment. The photosynthesis rate steadily increased after leaf development, reaching 20.8 μmol CO2·m-2·s-1 on the 10 days, gradually increasing to 21.3 μmol CO2·m-2·s-1 on the 19 days, and reaching 23.4 μmol CO2·m-2·s-1 on the 32 days. After that, it lowered to 16.8 μmol CO2·m-2·s-1 on the 38 days and dropped significantly to 7.6 μmol CO2·m-2·s-1 on the 47 days. As a result of the fruit characteristics by fruiting nodes, the treatments of the fruit length was 12.6-13.4 cm, respectively, which was significant, and the fruit width was 7.9- 8.6 cm, respectively, was not significant. The soluble content ranged from 12.9 to 15.7°Brix, and the significance of all treatments, and higher than of LF-DW and HF-UW. The photosynthesis rate of melon leaves was good until 32 days after leaf development, but after that, the rate decreased. As for fruit quality, it was conformed that melons can be cultivated at the LF because the fruit enlargement and soluble content dose not decrease even when set at the LF. Results indicated that those can be used for LF and defoliation in the development of vertical and hydroponic cultivation method in high-temperature season. 본 연구는 송풍 덕트을 이용하여 고온기 냉방과 수직 ∙ 수경재배 시 잎의 엽령별 광합성능력과 착과 절위별 과실 특성을분석하여 새로운 재배법 개발에 활용하고자 하였다. 참외 잎의 엽령은 3일 간격으로 광합성능력을 측정하였고, 착과 절위는 아들덩굴 5마디 이하에서 발생되는 손자덩굴을 모두 제거하는 처리(대조구), 아들덩굴 1마디에서 발생되는 손자덩굴에 착과 처리(저절위)를 하였다. 수직재배 시 줄기 유인을 아래에서 위로 유인하는 처리(상향), 위에서 아래로 유인하는 처리(하향)로 하였다. 광합성속도는 잎 전개 후부터 꾸준하게증가하여 10일에 20.8μmol CO2·m-2·s-1이었고, 19일에 21.3 μmol CO2·m-2·s-1로 조금씩 높아지더니, 32일에 23.4μmol CO2·m-2·s-1로 높았다. 그 이후 38일에는 16.8μmol CO2·m-2· s-1 낮아지고, 47일에는 7.6μmol CO2·m-2·s-1로 크게 낮아졌다. 착과 절위별 과실 특성은 저절위(상향), 저절위(하향), 대조구(상향), 대조구(하향)의 과장은 12.6-13.4cm로 유의성이 있었으며, 과폭은 7.9-8.6cm으로 유의성이 없었다. 과중은 저절위(상향)과 대조구(하향)에 비교하여 저절위(하향) 535.8g과 대조구(상향) 503.8g으로 유의성이 높았다. 당도는12.9-15.7°Brix로 저절위(상향)과 대조구(하향)의 유의성이 저절위(상향)과 대조구(하향) 보다 높았다. 참외 고온기 수직 ∙ 수경재배 시 참외 잎의 광합성능력은 잎 전개 후 32일까지는 좋지만, 그 이후에는 속도가 떨어지는 것으로 나타났다. 과실품질은 저절위 착과 시에도 과실비대와 당도가 떨어지지 않기 때문에 저절위에 착과하여 참외을 재배할 수 있음을 확인하였다. 본연구결과는 참외의 고온기 수직 ∙ 수경재배법 개발에 저절위 착과와 적엽에 활용할 수 있을 것으로 판단된다.

전자선 조사한 건조된 오징어와 문어에서 유도된 지방분해산물의 분석

홍영신,류근영,김경수 한국식품저장유통학회 2014 한국식품저장유통학회지 Vol.21 No.3

식품의 방사선 조사는 식품 저장을 위한 성공적인 현대 기술의 하나이지만 매우 신중하게 이루어져야 한다. 조사된 식품의 확인은 국제무역에서 소비자의 안전한 선택과 보호를 위해 가장 중요하다. 따라서 본 연구는 건조된 오징어와 문어에 전자선을 조사하여 선량별로 유도 생성되는 hydrocarbon류의 함량 및 분해패턴을 확인하였다. 비조사 시료와 선량별로 전자선 조사된 시료에서 지방을 추출한 뒤 solid phase extraction(SPE)방법을 이용하여 GC/MS로 확인하였다. 1, 3, 5, 7 및 10 kGy의 선량으로 전자선 조사된 건조 오징어와 문어에서 유도된 hydrocarbon류는 동일 선량에서 시료들의 지방산 조성에 따른 다른 생성률을 나타내었으며, 조사선량에 따라 증가하였다. 시료들에 다량 함유되어 있는 palmitic acid와 stearic acid로부터 생성된 1-tetradecene(C14:1), pentadecane(C15:0), 그리고 1-hexadecene (C16:1), heptadecane(C17:0)이 전자선 조사에 의해 유도된 주요 hydrocarbon류로 확인되었으며, 건오징어에서 hydrocarbon류 생성량이 건문어의 hydrocarbon류 생성량 보다 더 많음을 확인하였다. 이는 시료의 지방산 함유량에 따라 hydrocarbon류 생성량이 비례함을 알 수 있었다. 전자선 조사된 건오징어와 건문어에서 분석된 hydrocarbon류 중 1-tetradecene(C14:1), 1-hexadecene(C16:1) 그리고 heptadecane (C17:0)은 전자선 조사여부를 판별할 수 있는 지표로 활용 될 수 있을 거라 사료된다.

Monitoring of Biogas Yield from Livestock Manure at an Anaerobic Digestion Facility

홍영신,손진관,장재경,문종필,윤성욱 한국산학기술학회 2023 한국산학기술학회논문지 Vol.24 No.11

This study was conducted to examine the development and implementation of a system equipped with microbial electrochemical technology to reduce environmental impact and methane yield. Daily data produced by a complex gas measuring system installed in the digestion unit were analyzed. In 2019, 44,239 tons of livestock manure and 6,397 tons of food waste were processed, and the amount of biogas produced by anaerobic digestion was 2,471,243 N㎥. In 2020, 37,048 tons of livestock manure and 6,208 tons of food waste were processed, and the amount of biogas produced by anaerobic digestion was 1,930,030 N㎥. Monitoring of biogas in the anaerobic digester showed average CH4 and H2S concentrations were 79.97 % and 2,116 ppm in winters and 62.01 % and 2,309 ppm in summers, respectively. The results of this study would be helpful for researchers exploring ways to improve methane yields efficiency by stabilizing methane yields and applying microbial electrochemical technology to agricultural and livestock waste processing. Monitoring changes in biogas concentrations produced by anaerobic digestion on a seasonal basis could be used to predict the amount of biogas produced.

파프리카 수경재배 시 EC 농도와 배지에 따른 생육 및 수량 특성

홍영신,이재수,백정현,이상규,정선옥,Hong, Youngsin,Lee, Jaesu,Baek, Jeonghyun,Lee, Sanggyu,Chung, Sunok 한국환경과학회 2021 한국환경과학회지 Vol.30 No.8

Supply electrical conductivity (EC) concentration of the nutrition solution is an important factor in the absorption of nutrients by plants and the management of the root zone, as it can control the vegetative/reproductive growth of a plant. Paprika usually undergoes its reproductive and vegetative growth simultaneously. Therefore, ensuring proper growth of the plant leads to increased yield of paprika. In this study, growth characteristics of paprika were examined according to the EC concentration of a coir and a rockwool substrate. The supply EC was 1.0, 2.0, and 4.0 mS·cm^-1 applied at the initial stages of the growth using the rockwool (commonly used by paprika farmers) and the coir substrate with a chip and dust ratio of 50:50 and 70:30. For up to 16 weeks of paprika growth, EC concentrations of 1.0 and 2.0 mS·cm^-1 were found to have a greater effect on the growth than EC at 4.0 mS·cm^-1. The normality (marketable) rate of fruit, the soluble solid content, and paprika growth showed that the coir was generally better than the rockwool regardless of the supply EC concentration. The values of the yield per plant at an EC concentration of 4.0 mS·cm^-1 was mostly similar at 1.6 kg (coir 50:50), 1.5 kg (coir 70:30) and 1.5 kg (rockwool), but the yield of the rockwool was 88%, which was lower than 98% and 94% yield of the coir substrate. Therefore, this concludes that coir substrate is more effective than rockwool at improving paprika productivity. The results also suggest that the use of coir substrate for paprika has many benefits in terms of reducing production costs and preventing environmental destruction during post-processing.

한우 암소의 부위별 뼈 육수 추출시간에 따른 무기 성분의 변화

홍영신,정지영,손지현,송옥연,황인민,Md. Atikul Islam,김희은,김경수 한국식품조리과학회 2019 한국식품조리과학회지 Vol.35 No.5

Purpose: This study was designed to determined the changes of inorganic components in Hanwoo cow bone (shank, sacrum, feet and miscellaneous) according to the different extraction times of bone broth. Method: The extraction time of bone broth was up to 14 hours, and the broth was sampled every 2 hours. The macro (Na, S, K, Ca, P and Mg) and micro (Mo, Cr, Mn, Cu, Rb, V, Li, Ba, Sr, Ga and Co) inorganic components were analyzed by ICP-OES and ICP-MS respectively. Results: The highest amount of Na was found in all parts of Hanwoo cow bone. The amount of S and Na were most abundant in feet, whereas the K, P, Ca and Mg were the highest in the miscellaneous bones. The quantity of Ca is increased until 14 hours of heating, but the quantity of Na, K, P, S and Mg were increased until heating for 12 hours. The micro mineral (Mo) was the highest in sacrum broth and it was increased by heating up to 14 hours. Conclusion: The quantity of macro and micro inorganic components varied according to the bones of Hanwoo cows and it is depending on bone of Hanwoo cow. Extracted inorganic matter with heating time was found to be different according to kinds of inorganic components. Based on the above results, it was determined that the optimum heating time for Hanwoo cow bone broth was 12 hours.