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헤스페레틴(Hesperetin)과 사이클로덱스트린(Cyclodextrin) 포접 복합체의 항산화, 항염증, 항균 활성
최성숙,이경애 한국응용과학기술학회 2023 한국응용과학기술학회지 Vol.40 No.5
Hesperetin(HT) is a potent antioxidant flavonoid aglycone derived from hesperidin(HD). The antioxidant, anti-inflammatory, and antimicrobial activities of HT and its cyclodextrin(CD) inclusion complexes were compared in vitro. HT was prepared by enzymatic hydrolysis of HD, and HT/CD complexes were prepared using β-cyclodextrin(β-CD) and hydroxypropyl-βcyclodextrin(HP-β-CD) by solvent co-evaporation method. The solubility of the HT/HP-β-CD inclusion complex increased 93.5-fold compared to HT, and the solubility of HT/β-CD increased 22.5-fold. The HT/HP-β-CD inclusion complex showed a similar effect as HT on radical scavenging activity in antioxidant assays, whereas the HT/β-CD inclusion complex showed slightly lower activity than HT. Cytotoxicity was low in the following order; HT/HP-β-CD, HT/β-CD, and HT in murine macrophage RAW264.7 cells. Treatment with HT and HT/CD inclusion complexes reduced the levels of inflammatory mediators such as nitric oxide(NO), tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) in the cells. HT and HT/HP-β-CD inclusion complex were more effective than HT/β-CD inclusion complex at relatively low concentrations. Inhibitory effects were tested on skin-pathogenic bacteria, Staphylococcus aureus and Pseudomonas aeruginosa, and they showed an antimicrobial effect on S. aureus in the order of HT = HT/HP-β-CD > HT/β-CD, but they did not show any significant inhibitory effect on P. aeruginosa. In conclusion, HT, the aglycone form of HD, and its CD inclusion complexes showed various biological activities. HT/HP-β-CD inclusion complex, which is the highly soluble form of HT, showed relatively higher activity compared to HT/β-CD inclusion complex.