F344 랫드를 이용한 이황화메틸의 아급성 흡입독성연구

김현영,이성배,한정희,정용현,김형진,신진영,신동호,김종춘,이용묵,Kim Hyeon-Yeong,Lee Sung-Bae,Han Jung-Hee,Chung Yong-Hyun,Kim Hyoung-Chin,Shin Jin-Young,Shin Dong-Ho,Kim Jong-Choon,Lee Young-Mook 한국생명과학회 2005 생명과학회지 Vol.15 No.1

이황화메틸의 반복 흡입노출에 의한 아급성 독성 잠재력을 평가하기 위해 암수 랫드에게 0, 5, 25 및 125 ppm용량으로 21일간 반복 흡입노출하고, 일반증상과 체중, 사료섭취 량, 혈액치, 혈청생화학치 및 부검소견을 관찰하였다. 시험결과, 랫드에게 이황화메틸을 3주간 반복 흡입노출하면 125 ppm의 농도에서 체중증가의 억제와 사료섭취 량의 감소를 유발하나, 혈액 및 혈청생화학치에는 어떠한 이상도 유발하지 않는 것으로 나타났다. 본 시험 조건 하에서 이황화메틸의 표적 장기는 관찰되지 않았으며, 무해용량은 암수 모두 25 ppm으로 사료된다. The purpose of this study was to investigate the potential subacute toxicity of dimethyl disulfide by 3 weeks inhalation in F344 rats. The test article, dimethyl disulfide was exposed by inhalation to male and female rats at dose levels of 0, 5, 25, or 125 ppm/6 hrs/ day for 3 weeks. Five rats/ sex/ group were sacrificed on day 4 after the initiation of treatment, while 5 rats/ sex/ group were sacrificed at the end of treatment period. During the test period, clinical signs, mortality, body weights, food consumption, hematology, serum biochemistry, and gross findings were examined. Slight decreases in body weight gain were noted in both sexes of the highest dose group in a dose dependent manner but were only statistically different from the control animals in males of the group. A slight non-significant reduction in food consumption were also noted in the both sexes of the highest dose group. There were no adverse effects on mortality, clinical signs, hematology, serum biochemistry, and necropsy findings at any dose tested. Based on these results, it was concluded that the 3 weeks repeated dose of dimethyl disulfide by inhalation resulted in suppressed body weight gain and decreased food consumption at 125 ppm of both sexes. In the present experimental conditions, the target organ was not determined in rats. The no­observed-adverse-effect level (NOAEL) was considered to be 25 ppm/6 hrs/day for both sexes.

경남지역 전통 된장의 숙성기간에 따른 품질 특성 및 미생물 군집 비교

김현영,김봉신,고희숙,김소영,하기정,Kim, Hyeon-Young,Kim, Bong Sin,Ko, Hee-Suk,Kim, So-young,Ha, Gi-Jeong 한국식품영양학회 2021 韓國食品營養學會誌 Vol.34 No.1

This study investigated the microbial community and quality characteristics of traditional soybean pastes (doenjang) based on the aging period in Gyeongnam province. The samples were collected from seven regions in Gyeongnam Province (Goseong, Hadong, Haman, Hamyang, Sacheon, Sancheong and Uiryeong). Contents of moisture, salinity, pH, and acidity of doenjang after 210 days aging were 56.40~65.21%, 9.05~16.08%, 4.88~6.86 and 0.64~2.14%, respectively. Lightness significantly decreased over the aging period, while the redness tended to increase over the aging period. Yellowness was from 21.39~26.81 to 21.10~28.36. Reducing sugar content was from 0.31~1.45% to 0.11~3.13%. The amino-type content increased from 141.87~495.13 mg/100 g to 328.53~823.67 mg/100 g. In contrast, the ammonia-type content declined from 136.74~364.70 mg/ 100 g to 128.62~331.00 mg/100 g. The overall total microbial count did not significantly differ, but it tended to decrease in sample GD4. Fungus declined overall or was not detected. Coliform groups were not detected in all samples. Lactobacillus and Bacillus cereus tended to decrease over the aging period. The common fungus and bacterium were Aspergillus oryzae and Tetragenococcus halophilus, respectively. After the aging period, the predominant fungi were Candida versatilis, Candida apicola, and Debaryomyces hansenii. The predominant bacteria were Tetragenococcus halophilus and Cronobacter sakazakii.

Mouse의 신장상피세포에서 패장(敗醬)추출물이 산화 스트레스 및 NF-${\kappa}B$ signaling에 미치는 영향

김현영,장수영,최규호,신현철,Kim, Hyun-Young,Jang, Soo-Young,Choi, Gyu-Ho,Shin, Hyeon-Cheol 대한한방내과학회 2010 大韓韓方內科學會誌 Vol.31 No.1

Objectives : The aims of this study were to investigate the cytoprotective, antioxidative and inflammation genes inhibitory effects of Patriniae Radix on the mouse LLC-$PK_1$ cells (renal epithelial cells). Methods : The cytoprotective effect of Patriniae Radix was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The antioxidative effect was measured in terms of generation amount of superoxide anion radical (${\cdot}{O_2}^-$) by 2',7'-dichlorodihydrofluorescein diacetate (DCFDA), nitric oxide (NO) by 4,5-diaminofluorescein (DAF-2), peroxynitrite ($ONOO^-$) by dihyldrorhodamine 123 (DHR 123) and prostaglandin $E_2$ ($PGE_2$) by $PGE_2$ immunoassay on $H_2O_2$-treated LLC-$PK_1$ cells. For measuring of inflammation genes inhibitory effects, western blot was performed to detect IKK-$\alpha$, phospho-$I{\kappa}B-\alpha$, NF-${\kappa}B$ (p50, p65), COX-2, iNOS, IL-$1{\beta}$ and VCAM-1 protein level in cytosol fractions from LLC-$PK_1$ cells. Results : Patriniae Radix extract reduced the $H_2O_2$-induced cell death and inhibited the amount of $H_2O_2$-induced ${\cdot}{O_2}^-$, NO, $ONOO^-$, $PGE_2$ generation dose-dependently on the mouse LLC-$PK_1$ cells in vitro. Also Patriniae Radix extract inhibited the expression of IKK-$\alpha$, phospho-$I{\kappa}B-\alpha$, COX-2, iNOS, IL-$1\beta$ and VCAM-1 genes dose-dependently by means of decreasing activation of NF-${\kappa}B$. Conclusions : According to above results, it was identified that Patriniae Radix had the cytoprotective, antioxidative and inflammation genes inhibitory effects. So it was suggested that Patriniae Radix would be effective to the treatment for the inflammatory process and inflammation-related diseases.

곡기생(?寄生)의 항염증 효능 및 암세포 이주저해에 미치는 영향

김현영,장수영,정지천,신현철,Kim, Hyun-Young,Jang, Soo-Young,Jeong, Ji-Cheon,Shin, Hyeon-Cheol 대한한방내과학회 2013 大韓韓方內科學會誌 Vol.34 No.1

Objectives : Gokgisaeng (Korean mistletoe) is used for the treatment of inflammatory and cancer diseases in traditional Korean medicine and its major component lectins have been reported to induce nitric oxide (NO) in RAW 264.7 macrophages, and also induce apoptosis of various types of cancer cells, although its modulatory effects on cancer cell migration and macrophage activation is poorly understood. The aim of this study is to clarify molecular mechanisms of action responsible for the anti-inflammatory and antitumor migration potentials of Korean mistletoe extract (KME). Methods : We investigated the anti-inflammatory activity of KME on NO production and inducible nitric oxide synthase (iNOS) expression by lipopolysaccharide (LPS) in both RAW 264.7 macrophages and rat C6 glioma cells, and also evaluated inhibitory efficacy on glioma cell growth and migration. For assessment, XTT assay, nitrite assay, RT-PCR, scratch-wound and Boyden chamber assay, and western blot analysis were performed. Results : Previously reported, unlike the efficacy of Gokgisaeng lectin, KME inhibited NO production and iNOS expression, and suppressed pro-inflammatory mediators including IL-$1{\beta}$, IL-6, COX-2, iNOS in LPS-stimulated RAW 264.7 cells. Furthermore, KME suppressed tumor cell growth and migration, and it also inhibited LPS-induced NO release and iNOS activation by down-regulating expression of protein kinase C (PKC) and phosphorylation of ERK in C6 glioma cells. Conclusions : Our research findings provide evidence that KME can play a significant role in blocking pro-inflammatory reaction and malignant progression of tumors through the suppression of NO/iNOS by down-regulating of inflammatory signaling pathways, PKC/ERK.

제주 지역 여성청년의 독립 경험에 관한 공간정치적 접근

김현영(Kim Hyeon-Young),김태연(Kim Tae-Yeon) 한국공간환경학회 2021 공간과 사회 Vol.31 No.4

본 연구는 제주 지역 여성청년의 독립 실천을 다루면서 중심부와 주변부의 공간위계가 상상되고 경합되는 과정의 역동을 살펴본다. 농촌보다는 도시를, 지방보다는 서울을 열망하는 공간위계 속에서 지역 청년들의 변화하는 생애주기에 대한 인식과 삶의 맥락은 어떻게 구성되고 있을까? 중심부와 주변부 공간의 물질적이고 상징적인 격차가 심화되는 가운데 지역, 젠더와 계급이라는 범주는 어떻게 각축하고 있을까? 이 질문에 답하기 위해, 본 연구는 독립과 의존을 대립적인 것으로 상정하는 대신 여성청년들의 독립 실천을 ‘과정적인 것’으로 규정하고, “잔류”로 표상되는 삶의 맥락 속에서 자신만의 공간을 확보하려는 다양한 실천을 살펴본다. 이들의 독립 실천은 중앙과 지방, 이주와 정주라는 이중의 위계 속에서 현재적 사건으로 긍정되지 못하며 다양한 구조적 곤란에 맞닥뜨리게 된다. 그럼에도 이들은 다양한 공간전략을 구사하며 새로운 장소를 만들어낸다. 관계적 장소로서 새롭게 집을 장소화하는 이들의 실천에는 중앙-지방의 공간위계를 새롭게 사유할 가능성이 잠재한다. This paper, considering the young unmarried women’s experiences of making their own space in Jeju Island, explores the ways of interpreting the hierarchical dualism of geographical center and periphery in South Korea. Our research question is how the young women who live in Jeju perceive and interpret their own life context, and then how they cope with spatial hierarchy between the urban and the rural, and between Seoul and the local. In some sense, their encounter with the spatial hierarchy cannot but help break through the effects of intermingling social forces such as locality, gender and class. The deepening gap of material (economic) opportunities and of symbolic power between the central and peripheral region is a big challenge to the young generation in Jeju. We define their practices of making their own living spaces as ‘processive’ and regard these as ‘remaining’ in their living context. Their practices are neglected in the cultural circumstance that forces the young generation to sacrifice the present for the future. That sacrifice is justified as an investment. It looks like an structurally imposed obstacle. Nevertheless, the young women, particularly unmarried women try to reclaim new places through negotiating between their position within the structure and their own strategic choice. It can be their detour through which they rethink the spatial hierarchy.

증례(證例) : 십이지장 선암으로 발생한 위출구 폐쇄 환자 치험 1례

김현영 ( Hyun Young Kim ),장수영 ( Soo Young Jang ),신현철 ( Hyeon Cheol Shin ) 대구한의대학교 제한동의학술원 2011 東西醫學 Vol.36 No.1

Gastric outlet obstruction is an obstruction at the level of the pylorus, so foods can`t pass into the small intestine. The main symptom is vomiting, which typically occurs after meals of undigested food. The cause have changed from peptic ulcer disease to malignant diseases in recent years. And the treatment depends on the underlying disease, but may include either medical therapies or surgery. In this report, we introduce a case of the gastric outlet obstruction caused by duodenal adenocarcinoma. The pateint was a 61-year-old woman that admitted because of nausea, vomiting for 3 weeks. For controlling the symptoms, she had taken Gamiwhajung-Eum, Gamibaegi-Eum. Then nausea, vomiting were reduced, but we couldn`t control them perfectly. Therefore, We suggests that oriental treatment and western medical surgery, both are needed at gastric outlet obstruction caused by malignant disease.

‘사회재생산의 위기’를 둘러싼 페미니즘 논의의 검토

김현영(Kim, Hyeon-Young) 한국여성연구소 2022 페미니즘 연구 Vol.22 No.2

이 글은 오늘날 사회재생산의 위기를 이해하기 위해 페미니즘 문헌에서 사회재생산에 관한 이론적 논의를 검토한다. 우선, 가사노동 논쟁을 거쳐 분석적 프레임워크로서 사회재생산 개념의 발전과정을 고찰한다. 이렇게 도출된 사회재생산 관점을 통해 신자유주의적 지구화와 변화하는 젠더질서 속에서 이중화되는 사회재생산의 양상을 검토한다. 이는 탈젠더화되고 탈인종화된 특성을 표방하는 신자유주의적 경제가 사실상 젠더, 인종, 계층과 시민권의 교차 속에서 위계적으로 구성되는 사회재생산 노동에 깊숙이 의존하고 있음을 드러낸다. 오늘날 재생산노동의 위계적 조직화가 여성내부의 격차를 체계적인 방식으로 결합하며 이루어진다는 사실은 방법론적 일국주의를 넘어선 교차적인 사회재생산 분석의 발전을 요구한다. 마지막으로 이 같은 이론적 통찰이 생산과 재생산의 경계를 문제삼는 페미니즘 정치에 제기하는 시사점을 간략히 살펴본다. This study examines the trajectory of ‘social reproduction’ in the context of feminist literature since the domestic labour debate. It analyzes the privatization of social reproduction and shifting gender order in the neoliberal era and emphasizes that the neoliberal economy which claims to be degendered and deracailized, is highly dependent on the hierarchical construction of social reproductive labour with the intersection of gender, race class, and citizenships. It demands elaborations of intersectional social reproduction analysis beyond methodological nationalism. Finally, it suggests these theoretical insights could be useful resources for feminist politics.

COVID-19 언택트 시대의 보건계열 대학생 실습교육 만족도, 대학생활 적응 및 취업 불안감 관계

김현영(Hyeon-Young Kim),이은혜(Eun-Hye Lee) 한국콘텐츠학회 2023 한국콘텐츠학회논문지 Vol.23 No.1

병원 간호사의 간호대학생 교육 경험

김현영(Kim, Hyeon-Young),이한나(Lee, Hanna) 한국웰니스학회 2021 한국웰니스학회지 Vol.16 No.2

본 연구의 목적은 병원 임상에서 근무하는 간호사가 간호대학생을 교육하면서 겪는 경험세계를 주관적이고 총체적으로 통찰하고 발견하여 해석하고자 한다. 본 연구의 대상은 대학병원이나 종합병원의 간호사로 근무 중이면서 5년 이상의 병원경력과 1년 이상의 임상실습 교육을 경험하였으며, 마지막 실습교육 경험이 1년이 지나지 않은 자로 본 연구에 참여하기로 수락한 간호사 6명을 대상으로 심층면담을 실시하였다. 면담자료는 전사하여 Colaizzi의 현상학적 분석방법으로 분석하였다. 연구 결과 3개의 범주, 7개의 주제 모음, 18개의 주제로 분류되었으며, 3개의 범주는 ‘업무에 더해진 짐’, ‘제약된 임상교육 현장’, ‘학생과 함께 성장’으로 나타났다. 본 연구는 간호대학생을 교육하는 데 있어 병원과 학교, 간호사가 앞으로 나아갈 방향을 제시하기 위한 기초자료로 활용될 수 있다. The purpose of this study was to gain insight, discover, and interpret the world of experience gained by nurses who worked in clinical practice while educating nursing college students in a subjective and comprehensive manner. The subjects of this study were those who worked as nurses at university hospitals or general hospitals, had experienced more than 5 years of working in a hospital and more than 1 year of clinical practice education, and who had not passed 1 year of their last practice education experience. A total of 6 nurses accepted to participate in this study, and they were interviewed in-depth. Interview data were transcribed and analyzed by Colaizzi’s phenomenological method. The results were classified into three categories, a collection of seven theme cluster, and eighteen themes and they were “a burden added to work,” “constrained clinical education field,” and “growth with students.” This study can be used as basic data to suggest future directions for hospitals, schools, and nurses in educating nursing college students.

<종옥전>을 활용한 문학치료 사례 연구 -“남녀서사”의 진단과 치료-

김현영 ( Hyeon Young Kim ) 겨레어문학회 2010 겨레어문학 Vol.44 No.-

This paper is a case report about Literary therapy counseling of <Jongokjeon>. In chapter 2, I discussed the aspects of epic found in <Jongokjeon>. In the novel, I could see, first, man and woman epic: the epic of recovering the opposite sex, and second, self-actualization, which resulted from the analysis based on Jung` analytical psychology. Consequently, this shows that in <Jongokjeon>, thiere is the epic of a success on human relationships. In chapter 3, I mentioned the process and result of the Literary therapy counsel using <Jongokjeon>. In this case, the subjects were two high school students, and the process was four steps of preparation → diagnosis→ therapy → finish. The students, at first, had self-epic of the dividing the opposite sex epic, rejecting the epic of recovering the opposite sex on <Jongokjeon>. However, their self-epic has changed step by step passing through the therapy stage. In this stage, I made use of a stepping stone epic, a movie <What`s Eating Gilbert Grape>. Experiencing the movie, the students changed their attitude toward the opposite sex. They became to accept the recovering the opposite sex epic of <Jongokjeon>. And, with the activity of writing their own Lifetime, students showed some changes in their self-epic on <Jongokjeon>. With this case, in short, I could see that the Literary therapy using <Jongokjeon> is valid and useful to some extent.