Heme oxygenase-1 induced by desoxo-narchinol-A attenuated the severity of acute pancreatitis via blockade of neutrophil infiltration

Bae, Gi-Sang,Kim, Dong-Goo,Jo, Il-Joo,Choi, Sun-Bok,Kim, Myoung-Jin,Shin, Joon Yeon,Kim, Dong-Uk,Song, Ho-Joon,Joo, Myungsoo,Park, Sung-Joo ELSEVIER 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.69 No.-

<P><B>Abstract</B></P> <P>Heme oxygenase-1 (HO-1) has an anti-inflammatory action in acute pancreatitis (AP). However, its mechanism of action and natural compounds/drugs to induce HO-1 in pancreas are not well understood. In this study, we investigated the regulatory mechanisms of HO-1 during AP using desoxo-narchinol-A (DN), the natural compound inducing HO-1 in the pancreas. Female C57/BL6 Mice were intraperitoneally injected with supramaximal concentrations of cerulein (50 μg/kg) hourly for 6 h to induce AP. DMSO or DN was administered intraperitoneally, then mice were sacrificed 6 h after the final cerulein injection. Administration of DN increased pancreatic HO-1 expression through activation of activating protein-1, mediated by mitogen-activated protein kinases. Furthermore, DN treatment reduced the pancreatic weight-to-body weight ratio as well as production of digestive enzymes and pro-inflammatory cytokines. Inhibition of HO-1 by tin protoporphyrin IX abolished the protective effects of DN on pancreatic damage. Additionally, DN treatment inhibited neutrophil infiltration into the pancreas via regulation of chemokine (C-X-C motif) ligand 2 (CXCL2) by HO-1. Our results suggest that DN is an effective inducer of HO-1 in the pancreas, and that HO-1 regulates neutrophil infiltration in AP via CXCL2 inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Desoxo-narchinol-A (DN) is a natural compound of HO-1 inducer in pancreas. </LI> <LI> Mechanism of DN-induced HO-1 is mediated by MAPK/Activator Protein-1/HO-1 signaling. </LI> <LI> DN-induced HO-1 blocks neutrophil infiltration into pancreas via inhibition of CXCL2. </LI> <LI> DN inhibits cerulein-induced acute pancreatitis (AP) and AP-associated lung injury. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

동의보감 탕액편에 기재된 식이본초의 독성유무에 대한 분류 연구

신호동(Ho-Dong Shin),정종운(Jong-Un Jeong) 대한한의학회 2011 대한한의학회지 Vol.32 No.1

혈관질환 정보관리 시스템

김동익,김덕경,허세호,이병붕,김용신,김은숙,문지영,도영수,신성욱,김동수,김만태,진재욱,김용신 대한혈관외과학회 2002 Vascular Specialist International Vol.18 No.1

As medical technology progresses rapidly, there is a rise in the average age along with the Korean dietary lifestyle becoming more westernized, which leads to an increase in the number of vascular disease patients in Korea. Thus, we need to manage the medical information of a disease systematically in order to diagnose and treat constructively. However, since there has been no standardized method of man agement to date, a great deal of information could not be properly utilized nor studied. Therefore, the departments of Cardiology, Radiology and Neurology of Samsung Seoul Hospital recently got together to develop an information management system called the Vascular Data System. This program was developed to be run on win98 O/S, upper Pentium Ⅲ, and upper 128 MB Memory, and its source code is Dephi 4.0. It was configured for the user to set the configurations as well as do a variety of search and analysis. If this program were to be updated continuously, it may be used extensively as well as in various parts of clinical research activities.

Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Lee, Sang Choel,Han, Seung Hyeok,Li, Jin Ji,Lee, Sun Ha,Jung, Dong-Sub,Kwak, Seung-Jae,Kim, Seung Hye,Kim, Dong Ki,Yoo, Tae-Hyun,Kim, Jin Hyun,Chang, Se-Ho,Han, Dae Suk,Kang, Shin-Wook International Society of Nephrology 2009 Kidney international Vol.76 No.8

Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose–treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose–treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.

만성 견비통에 대한 동씨침 치료의 무작위 대조군의 임상 연구

김찬영,권나현,신예지,남동우,김건형,김종인,최도영,이윤호,이재동 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2007 東西醫學硏究所 論文集 Vol.2007 No.-

Objectives : To observe the effect of acupuncture treatment in chronic shoulder pain patients. Methods : 36 voluntary patients were randomly assigned to an acupuncture treatment group(E GrouP, n=18) and a control group(C GrouP, n=18). The E Group patients received acupuncture treatment on LI_(15), TE_(14), GB_(21) and Master Dong's acupuncture points, Shin-gwan and Gyun-joong, twice a week for four weeks. The C Group patients received no treatment. All patients in both groups were instructed to practise self exercise in their daily lives. Evaluations were made at baseline and after four weeks of study. The Constant Shoulder Assessment(CSA), Shoulder Pain and Disability Index(SPADI) and the patient's subjective pain was measured by Visual Analogue Scale(VAS). The obtained data was analyzed. Results '. The E Group showed significant(p<0.05) improvement in CSA, SPADI and VAS after four weeks of treatment. The C Group showed significant(p<0.05) improvement in CSA, but the change of SPADI and VAS was insignificant(P>0.05). CSA and SPADI of E Group significantly(p<0.05) improved compared to the C GrouP, but the difference of VAS change in the two groups was insignificant(p>0.05). Conclusions : Four weeks of acupuncture treatment significantly improved CSA, SPADI and VAS. The improvement of CSA and SPADI was significant(p<0.05) compared to untreated patients.

SM45C의 레이저 표면경화특성

신호준,유영태,안동규,임기건 한국공작기계학회 2005 한국공작기계학회 춘계학술대회논문집 Vol.2005 No.-

Laser surface hardening is an effective technique used to improve the tribological properties and also to increase the service life of automobile components such as camshafts, crankshatfs, lorry brake drums and gears. High power CO₂ lasers and Nd:YAG lasers are employed for localized hardening of materials and hence are of potential application in the automobile industries. The heat is conducted rapidly into the bulk of the specimen causing self quenching to occur and the formation of martensitic structure. In this investigation, the microstructure features occurring in Nd:YAG laser hardening SM45C steel are discussed with the use of optical microscopic and scanning electron microscopic analysis. Moreover, This paper describes the optimism of the processing parameters for maximum hardened depth of SM45C steel specimens of 3mm thickness by using CW Nd:YAG laser. Travel speed was varied from 0.6m/min to 1.0m/min. The maximum hardness and case depth fo SM45C steel are 780Hv and 0.4mm by laser hardening.

독일 도르트문트시의 지역혁신체제 : 첨단산업단지 중소기업 지원기관을 사례로 The Roles of Supporting Institutions of Firms in Dortmund Technology Park

신동호 한국경제지리학회 2004 한국경제지리학회지 Vol.7 No.3

독일의 루르 지역은 석탄과 철강 산업의 중심지로 독일이 부흥하는데 중요한 역할을 하였다. 도르트문트시는 루르지역의 한 중심도시로, 1970년대 초까지 급격한 성장을 계속하였다. 그러나 그 후부터 탈산업화 과정을 겪으면서 실업문제와 환경오염 등으로 도시의 사회, 경제적 여건이 열악해지는 상황에 직면하게 되었다. 도로트문트시는 이러한 문제를 타개하기 위해 1985년부터 도르트문트 대학에 창업보육센타와 과학기술연구공원을 조성하는 등, 산업구조의 고도화를 위해 적극적으로 노력하였다. 본 연구는 도르트문트시가 추진한 이러한 경제구조 개편정책을 지역혁신이란 맥락에서 분석하고자 하는 바, 1970년대 이후 일어난 도시경제 구조에 있어서의 전반적인 변화를 분석하고, 과학기술 연구공원에 입주한 기술집약적 중소기업과 각종 기업지원 혁신주체를 대상으로 면담조사를 실시하였고, 또 32개의 업체를 대상으로 설문조사를 실시하였다. 이러한 조사에 근거하여 본 연구는 도르트문트가 효과적인 지역혁신체제를 구축하긴 하였지만 아직 실리콘밸리나 제3의 이탈리아와 같은 수준의 혁신환경을 조성하는 수준에는 달하지 못하였다고 주장한다. A core industrial city of Germany, Dortmund, has gone through a serious deindustrialization process since 1960s. It lost more than 50,000 jobs from the two major industries of coal mining and iron and steel manufacturing during the period between the mid-1970s and the early 1990s. To promote new industries by providing new industrial spaces and technological and managerial assistance, Dortmund created a technology park and competence centers. Some dedicated entrepreneurs and scientists organized business organizations to create innovative milieu from which firms of the technology park. This paper reviews the roles of assisting organizations and their effectiveness to conclude Dortmund successfully established an effective mechanism of regional innovation. The city, however, yet to want to see innovative milieu, to the levels that have been shown by Silicon Valley and the Third Italy.

惠施 逆設의 意味

申東浩 충남대학교 인문과학연구소 1979 인문학연구 Vol.6 No.1

In this paper, I have examined some methods for interpretation of the Hui Shih's seven propositions which are generally considered most paradoxical among his ten propositions, and suggested which one is most appropriate for the right interpretation. His underlying meaning through these paradoxical statements are as follows: Hui Shih indicated the relative limitation, thorugh his paradoxes, of our empirical knowledge that would compare and measure the aspects of spatial things or the progress of time. Moreover he elucidated paradoxically the blindpoints of common ideas about the natural phenomena and the meaning of our language which were accepted being self-evident in our lives.

American Medical Association 5판을 근거로 한 한국형 말초신경계 장애 평가기준 개발

박동식,김병조,황선철,송인욱,손병철,장성호,정두신 순천향대학교 의학연구소 2009 Journal of Soonchunhyang Medical Science Vol.14 No.3

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브라켓 접착시 광중합방식에 따른 전단결합강도와 파절양상 비교

신재호,임용규,이동렬 대한치과교정학회 2004 대한치과교정학회지 Vol.34 No.4

기존의 가시광선 중합기와 비교해서 plasma arc light와 LED 방식의 중합기를 이용해 브라켓을 치아표면에 접착한 후 탈락시키는 실험을 시행해 각각의 중합방식별로 전단결합강도와 접착파절양상을 비교함으로써 임상에서의 유용성을 평가하고자 하였다. 교정치료를 위해 발거한 상, 하악 소구치 60개를 윈통형의 레진블록에 매몰하여 시편을 제작하였다. 광중합형 접착레진인 Transbond XT를 이용하여 광중합 방식별로 조건(가시광선 중합기는 40초, LED방식의 중합기는 20초, 그리고 plasma arc light 방식의 중합기는 3초) 에 따라 브라켓을 접착한 후 만능물성실험기로 전단결합강도를 측정하고 브라켓 기저면을 광학현미경으로 관찰해 접착파절양상을 관찰하여 다음과 같은 결과를 얻었다. 1. 가시광선 중합방식과 LED 중합방식으로 중합시킨 군간에 전단결합강도의 유의성 있는 차이는 없지만 plasma arc Light 방식으로 중합시킨 군은 앞의 두 군에 비해 유의성이 있게 작은 값을 나타내었다(p>0.05). 2. 가시광선 중합방식으로 중합한 군과 LED 방식을 이용한 군에서는 파절양상이 거의 유사하게 나타났다. 두 군 모두 잔류접착제가 치아면에만 있는 경우가 제일 적은 비율을 보였으며 브라켓 기저면에 50% 이상의 접착제가 남아 있는 양상이 더 큰 비율을 보였다. 3. Plasma arc light 로 중합시킨 경우에는 접착제가 브라켓 기저면에 50% 이하로 남아있는 양상이 큰 비율로 나타났으며 잔류 접착제 전체가 치아면에 남아 있는 경우는 15%였다. 이상의 실험결과 plasma arc light를 이용한 중합 방식이 가시광선이나 LED 방식을 이용한 중합 방식에 비해 유의성 있게(p>0.05) 낮은 전단결합강도를 보였으나 세 방식 모두 임상적으로 사용하기에 충분한 전단결합강도를 보여 유용하게 사용할 수 있음을 알 수 있었다. The purpose of this study was to evaluate the clinical effectiveness of a plasma arc light and light emitting diode (LED), compared with shear bond strength and the failure pattern of brackets bonded with visible light in direct bonding. Brackets were bonded with Transbond XT to 60 human premolars embedded in the resin blocks according to different light-curing methods. Then, the shear bond strength of each group was measured using a universal testing machine (Instron) and the adhesive failure pattern after debonding was visually examined by light microscope. The results were as follows: 1. The shear bond strength showed no significant difference between the visible light and light emitting diode, but the plasma arc light exhibited a significantly lower shear bond strength compared with the visible light and light emitting diode. 2. In the visible light and light emitting diode, adhesive failure patterns were similar. Bond failure occurred more frequently at the enamel-adhesive interface. 3. The bonding failure of brackets bonded with plasma arc light occurred more frequently at the bracket-adhesive interface. The results of this study suggest that plasma arc light, light emitting diode and visible light are all clinically useful in the direct bonding of orthodontic brackets.