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개소시랑개비 추출물의 RAW264.7대식세포에서 in vitro 항염효과
남정환,김현삼,김병진,유홍섭,장동칠,진용익,유동림,최종근,박희준,이승빈,이경태,박수진,Nam, Jung-Hwan,Kim, Hyun-Sam,Kim, Byoumg-Jin,Yu, Hong-Seob,Chang, Dong-Chil,Jin, Yong-Ik,Yoo, Dong-Lim,Choi, Jong-Keun,Park, Hee-Jhun,Lee, Seung-Bin,Lee 한국식물생명공학회 2017 식물생명공학회지 Vol.44 No.1
본 연구에서는 개소시랑개비 (Potentilla supina)의 전초를 이용하여 세포독성 및 항염증 활성 효과를 평가하였다. 대식세포인 RAW264.7 cell에서 염증 매개 물질인 lipopo-lysacchride(LPS)로 염증을 유발해 nitric oxide (NO), Inducible nitric oxide synthase (iNOS)와 prostaglandin$E_2$($PGE_2$) 같은 염증 유발 인자들의 억제효과를 확인하였다. 개소시랑개비 ethyl acetate 분획물의 염증 유발 인자 억제 시 $IC_{80}$ value를 측정하였을 때 nitric oxide 및 prostaglandin $E_2$ 생성을 농도의존적으로 현저하게 저해하는 농도는 각각 29.34와 $50.75{\mu}g/ml$이었고 Inducible nitric oxide synthase의 양도 $50{\mu}g/ml$ 처리하였을때 농도 의존적으로 발현 감소 하였다. 따라서 본 연구결과는 개소시랑개비의 ethyl acetate 분획물과 같은 비극성용매 분획물들이 유의성 있는 항염증 효과를 나타내었으며, 이러한 효능은 예방의학적 가능성을 충분히 가지고 있기에 염증성질환의 예방을 위한 건강 기능성식품의 개발 가능성을 제시할 수 있을 것으로 기대된다. 또한 염증과 관련된 사이토카인 및 단백질 발현 메커니즘에 대한 추가적인 연구가 필요할 것으로 판단된다. Potentilla supina (Rosaceae) has traditionally been used to treat disorders of hemostasis, dysentery, malaria, bloody discharge and arthritis, and it has antinociceptive and anti-inflammatory properties. However, validity of the anti-inflammatory activity has not been scientifically investigated so far. Therefore, the aim of this study was to investigate the anti-inflammatory potential of P. supina using the ethanolic extract of P. supina and its sub-fractions. To evaluate the anti-inflammatory effects of P. supina, we examined the inflammatory mediators such as nitric oxide (NO), inducible nitric oxide synthase (iNOS) and prostaglandin $E_2$ ($PGE_2$) in RAW 264.7 cells. Our results indicated that ethyl acetate fraction significantly inhibited LPS-induced NO, iNOS and $PGE_2$ production in RAW 264.7 cells. This result showed that ethyl acetate fraction of P. supina is expected to be a good candidate for development into a source of anti-inflammatory agents.
남정환 ( Jung-hwan Nam ),김현삼 ( Hyun-sam Kim ),구본철 ( Bon-cheol Koo ),정진철 ( Jin-cheol Jeong ),서종택 ( Jong-taek Seo ),김율호 ( Yul-ho Kim ),김기덕 ( Ki-deog Kim ),이종남 ( Jong-nam Lee ),홍수영 ( Su-young Hong ),김수정 ( S 충북대학교 과학교육연구소 2016 과학교육연구논총 Vol.32 No.1
Recently, cvs `Hongyoung` with red color in skin and `Jayoung` with purple color in flesh potatoes were developed in Korea. Generally, it has been well known that the color of skin and flesh is due to an accumulation of anthocyanin that exhibit many functional properties for human health. In recent study, we found that extracts of two color-fleshed potatoes showed the antimutagenic effect and cytotoxity to human cancer cell lines, As functional properties of color-fleshed potatoes are widely introduced to consumers, it will be considered that the demand for the processing to various food materials including powder increase. This study was carried out to investigate the effect of steaming and drying conditions on the quality of the powders in color-fleshed potatoes. Cvs. `Hongyoung` and `Jyoung` and `Superior` as control were treated with different steaming times drying, room condition drying and hot air drying were treated. The quality factors such as anthocyanin content, moisture and hardness of powder were investigated. As a result of determining of Hunter`s value, powder color was well maintained after drying process by freeze drying for `Jayoung` and room condition drying for `Hongyoung`. anthocyanin in all cultivars as compared with other methods. The optimum hardness of powder was obtained in the steaming treatment for about 20 minutes before drying for all cultivars. Based on these results, our findings suggest that powders obtained from color-fleshed potatoes are able to be used for functional food materials.
개쇠랑개비(Potentilla supina)추출물의 항산화 활성 및 Total Polyphenol분석에 관한 연구
남정환 ( Jung-hwan Nam ),김현삼 ( Hyun-sam Kim ),구본철 ( Bon-cheol Koo ),정진철 ( Jin-cheol Jeong ),서종택 ( Jong-taek Seo ),김율호 ( Yul-ho Kim ),김기덕 ( Ki-deog Kim ),이종남 ( Jong-nam Lee ),홍수영 ( Su-young Hong ),김수정 ( S 충북대학교 과학교육연구소 2016 과학교육연구논총 Vol.32 No.1
The objectives of this study were to investigate the antioxidant activity and total poly phenol content from Potentilla supina extract and fraction, DPPH free radical scavenging activity was most excellent in the ethyl acetate fraction (95.4±0.3%) and ethanol extract(62.3±1.9%) of 250ug/m concentration, The ethanol extract (53.1±2.8mg/g), and the ethyl acetate fraction (316.4±11.8mg/g) showed relatively higher total polyphenol contents. There was the large amont of total polyphenol content in the ethanol extract and the ethly acetate fraction, and their free radical scavenging activities were higher effects.
RAW264.7 대식세포에서 풀솜대 추출물의 nitric oxide 및 prostaglandin E<sub>2</sub>생성 저해효과
남정환,서종택,김율호,김기덕,유동림,이종남,홍수영,김수정,손황배,김현삼,김보성,이경태,박희준,Nam, Jung-Hwan,Seo, Jong-Taek,Kim, Yul-Ho,Kim, Ki-Deog,Yoo, Dong-Lim,Lee, Jong-Nam,Hong, Su-Young,Kim, Su-Jeong,Sohn, Hwang-Bae,Kim, Hyun-Sam,Kim, B 한국식물생명공학회 2014 식물생명공학회지 Vol.41 No.4
본 연구에서는 풀솜대(Smilacina japonica)의 전초를 이용하여 세포독성 및 항염증 활성 효과를 평가하였다. 대식세포인 RAW264.7 cell에서 염증 매개 물질인 lipopo-lysacchride (LPS)로 염증을 유발시켜 nitric oxide (NO)와 prostaglandin $E_2$ ($PGE_2$) 같은 염증 유발인자들의 억제효과를 확인하였다. 풀솜대 chloroform 분획물의 염증 유발인자 억제 시 $IC_{80}$ value를 측정하였을 때 nitric oxide 및 prostaglandin $E_2$ 생성을 농도의존적으로 현저하게 저해하는 농도는 각각 53.3과 $32.5{\mu}g/ml$이었다. 따라서 본 연구 결과는 풀솜대의 chloroform과 같은 비극성용매 분획물들이 유의성 있는 항염증 효과를 나타내었으며, 이러한 효능은 예방의학적 가능성을 충분히 가지고 있기에 염증성질환의 예방을 위한 건강 기능성식품의 개발 가능성을 제시할 수 있을 것으로 기대된다. 또한 염증과 관련된 사이토카인 및 단백질 발현 메커니즘에 대한 추가적인 연구가 필요할 것으로 판단된다. Smilacina japonica is a localized common rhizomatous flowering plant, This plant is often used in Korean traditional systems of medicine as a remedy for migrain, diplegia, physical impurity, blood circulation, abscess and contusion. Generally drugs that are used for arthritis have antinociceptive and anti-inflammatory properties. However, validity of the anti-inflammatory activity has not been scientifically investigated so far. Therefore, the aim of this study was to investigate the anti-inflammatory potential of S. japonica using the ethanolic extract and its subfractions. To evaluate the anti-inflammatory effects, we examined the inflammatory mediators such as nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) on RAW 264.7 macrophages. Our results indicated that hexane fraction significantly inhibited the LPS induced NO and $PGE_2$ production in the cells. The hexane fractions inhibitory activity for NO tests with $IC_{50}$ values showed in $53.3{\mu}g/ml$ and $PGE_2$ tests with $IC_{50}$ values showed at $32.5{\mu}g/ml$. Theseis result suggest a potential role of hexane fraction from S. japonica as source of anti-inflammatory agent.
안주옥,김현삼 경희대학교 산학협력기술연구원 1998 산학협력기술연구논문집 Vol.4 No.-
Because cross-section of Prestressed Concrete Box Girder Bridge is usually a closed section, its transverse, longitudinal flexural rigidity and elastic stability are very high. Hence, the bridge is very safe and stable during construction and use, and frequently used in these days. It can be applied with advantages to 60=70m span and curved bridges that have a lot of torsional moments. The Box Girder Bridge transmits the upper weight to a pier and abutment through diaphragm built inside the box girder, and prevents the section deformation by increasing the cross-section stiffness. But, the diaphragm, which includes geometric and static discontinuity, causes a lot of problems to be accurately analyzed and reasonably designed only with Korean Design Codes. When a prestressed force ia applied directly to a diaphragm, a large tension force is built up opposite side and lower part of anchorage zone, which causes bursting stress. Cracks around the anchorage zone are found because of bursting stress. The bursting stress and the tension around the diaphragm are not considered in the Korean Design Code. Hence, the prestress transferring mechanism, the analysis of End Strut and the stability of Lower Corner, are needed to study. Analysis using Strut-and-Tie Model has been performed to transverse analysis of diaphragm. An accurate structural analysis about the anchorage zone has been performed by a jStrut-and-Tie Model based on Area-AASHTO and CEB-FIP code, and their results has been compared with those of the Korean Design Code. It has been found that the Korean Design Code underestimates the shear strength of concrete.
남경희,이기남,이준학,문준일,홍성주,김현삼 대한마취과학회 2000 Korean Journal of Anesthesiology Vol.38 No.3
Anesthesiologists are occasionally faced with a dilemma in managing Jehovah's witness patients who refuse transfusion of blood or blood products in life threatening situations. We report a case in which a 28-year old Jehovah's witness parturient underwent an emergency cesarean section due to complex problems of fetal distress, twin pregnancy and preeclampsia. Despite the severe anemia, she had a good postoperative recovery without any complications. We suggest that it is necessary to know the religious background, ethical issues and conflicts in caring for Jehovah's Witness patients, and be familliar with perioperative management without transfusion for these cases. (Korean J Anesthesiol 2000; 38: 567~ 570)