SCaM4 과발현 형질전환 감자의 농업적 특성 및 감자 무름병 저항성 평가

손황배,조광수,조지홍,권오근,천충기,최지경,이신우,정우식 한국식물생명공학회 2012 식물생명공학회지 Vol.39 No.4

We performed in vitro assay and field trials to assess levels of changes in intrinsic properties and resistance against soft rot of the potato cv. Dejima upon the introduction of a soybean calmodulin 4 gene (SCaM4). Field trials with four lines overexpressing SCaM4 gene were conducted over two seasons, and harvested tubers were evaluated in bioassay for resistance to Pectobacterium carotovorum ssp. carotovorum. The SCaM4 transgenic potato lines inoculated with 108CFU/ml of P. carotovorum ssp. carotovorum showed enhanced resistance compared to control. Among the SCaM4 transgenic lines, the transgenic line SCaM4-4 exhibited the highest tolerance to soft rot in vitro assays, so did in field trials. In the field trial, the soft rot resistance of SCaM4-4 line was more than 5 times higher compared to that of control cultivar,Dejima. The major agronomic characteristics of the SCaM4transgenic lines were not different from those of the nontransgenic ‘Dejima’. The result demonstrated that the transformation of a calmodulin 4 gene was a successful strategy in development of potato cultivar enhanced to soft rot. SCaM4 과발현 감자의 무름병에 대한 저항성은 대비 품종인 ‘대지’에 비해 실내・외 검정에서 저항성인 것으로나타났으며, SCaM4 과발현 SCaM4-4 계통의 경우 실내·외에서 무름병 저항성이 강하였다. SCaM4 과발현 형질전환 감자 계통들에 대한 포장 검정 결과 공시된 4 계통은 꽃색, 줄기형 등에서 대조품종인 대지와 차이가 없었다. 괴경특성 및 수량성의 경우 주당 괴경수는 유사했으나 과발현체의 경우 줄기 무름병 저항성으로 동화산물의생성 및 집적이 원활하여 괴경 무게 증가하여 수량이 증가하는 경향성을 보였다. 실내외검정에서 무름병에 강한저항성을 보인 SCaM4-4 라인은 대조품종인 대지와 실질적인 동등성을 확인할 수 있어 향후 식품학적 안전성과환경위해성 평가가 이루어진다면 실용화 가능성이 클 것으로 사료된다.

콩 전장 유전체 기반의 202개 InDel 마커를 활용한 한국 콩 품종의 염색체 재조합 양상 구명

손황배,김수정,구본철,김율호,황태영,박향미,이유영 한국육종학회 2017 한국육종학회지 Vol.49 No.3

Knowledge of the chromosomal constitution of the ancestors of modern soybean will complement plant breeding efforts to improve agronomic and economic characteristics of soybean. Variation block (VB)-based comparison using genome-wide insertion/deletion (InDel) markers was used on a diverse panel of 147 soybean cultivars to assess the impact of chromosomal changes during modern breeding. There were identical variation patterns of the examined InDels consistently appearing in the genome parts arising from parental varieties, indicating that soybean chromosomes in descendants should be all determined by genetic reshuffling of VBs inherited from parental chromosomes. Structure analysis of the accessions through the 202 InDels separated the accessions into four subgroups. Gene introgression revealed by the structure analysis agreed with the fact that a limited number of landraces and elite varieties were introduced and used as donors for breeding soybean cultivars in pedigree analysis. Especially, VBs became more reshuffled over time as a result of the breeding process, which resulted in using breeding parents with new VB-types for improving the end-use value of soybean. Therefore, their clustering using the 202 VB-specific InDels is strongly influenced by the difference in breeding ancestors among the subgroups. This indicates that the 202 InDel markers are very useful for genetic study by analyzing the reshuffling patterns of the parental genomes in the descendant.

[PG-0012] EMS 처리로 유기된 아미란스 유전자원의 주요 농업형질

손황배,김수정,김미향,남정환,김도연,이종남,홍수영,김율호 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

변이영역 특이 202개 InDel 마커를 이용한 강원도 육성 콩 품종의 판별 및 염색체 재조합 양상 구명

손황배,송윤호,김수정,홍수영,김기덕,구본철,김율호 한국육종학회 2018 한국육종학회지 Vol.50 No.4

The areas of soybean (Glycine max (L.) Merrill) cultivation in Gangwon-do have increased due to the growing demand for well-being foods. The soybean barcode system is a useful tool for cultivar identification and diversity analysis, which could be used in the seed production system for soybean cultivars. We genotyped cultivars using 202 insertion and deletion (InDel) markers specific to dense variation blocks (dVBs), and examined their ability to identify cultivars and analyze diversity by comparison to the database in the soybean barcode system. The genetic homology of “Cheonga,” “Gichan,” “Daewang,” “Haesal,” and “Gangil” to the 147 accessions was lower than 81.2%, demonstrating that these barcodes have potentiality in cultivar identification. Diversity analysis of one hundred and fifty-three soybean cultivars revealed four subgroups and one admixture (major allele frequency <0.6). Among the accessions, “Heugcheong,” “Hoban,” and “Cheonga” were included in subgroup 1 and “Gichan,” “Daewang,” “Haesal,” and “Gangil” in the admixture. The genetic regions of subgroups 3 and 4 in the admixture were reshuffled for early maturity and environmental tolerance, respectively, suggesting that soybean accessions with new dVB types should be developed to improve the value of soybean products to the end user. These results indicated that the two-dimensional barcodes of soybean cultivars enable not only genetic identification, but also management of genetic resources through diversity analysis.

국내 감자 24품종의 괴경내 글리코알칼로이드(glycoalkaloids) 함량 분포

손황배(Hwang-Bae Sohn),김수정(Su-Jeong Kim),이유영(Yu-Young Lee),박향미(Hyang-Mi Park),메카포구 만줄라타(Manjulatha Mekapogu),장동칠(Dong-Chil Chang),홍수영(Su-Young Hong),남정환(Jeong-Hwan Nam),서종택(Jong-Taek Suh),권기범(Ki-Bum Kweo 한국육종학회 2015 한국육종학회지 Vol.47 No.4

Potato glycoalkaloids (PGA) are potentially toxic to human at high levels and current safety regulations have recommended that PGA content in tubers should not exceed 20 mg/100g·FW. The purpose of this study was to evaluate α-chaconine, α-solanine and total PGA content in tuber peel and flesh of 24 potato cultivars including ‘Haryeoung’, ‘Goun’, ‘Hongyoung’ and ‘Jayoung’. The total PGA ranged from 3.1 to 10.1 mg/100g·FW and 41-85% of total PGA was accumulated in tuber peel in all cultivars. Potato cultivars with lower level of PGA were ‘Jasim’ (3.1 mg/100g·FW) and ‘Goun’ (3.4mg/100g·FW), whereas ‘Haryoung’ exhibited the highest level of PGA, 10.1 mg/100g·FW. Especially ‘Jasim’ and ‘Goun’ with the lower level of PGA can be consumed along with the peel on tubers. Also, these results provide the composition and levels of PGA in various potato cultivars for food safety and breeding of new cultivars with low levels of PGA for potato breeders and farmers.

컬러감자 안토시아닌 색소발현에 관여하는 재배환경 조건

정진철,김수정,홍수영,남정환,손황배,김율호,Manjulatha Mekapogu 한국작물학회 2015 한국작물학회지 Vol.60 No.2

This study was carried out to determine environmental factors affecting the anthocyanin content of colorfleshed potato (Solanum tuberosum L.) tubers. After planting of two color-fleshed potato cultivars of ‘Hongyoung’ and ‘Jayoung’ in different 14 locations, their soil chemical properties and meteorological data were evaluated, and anthocyanin contents of tubers were analyzed after harvest, additionally their relationship among them was analyzed through correlation analysis. In comparison with two cultivars, purple-fleshed ‘Jayoung’ potatoes showed higher anthocyanin content than red-fleshed ‘Hongyoung’ in almost locations. When locations were divided to three categories (highland, sub-highland and lowland) according to altitude, in general, highland-grown tubers had the higher content of anthocyanin compared to those grown in lowland. An analysis of the results of chemical components of soil showed that anthocyanin content of color-fleshed potato tubers was negatively correlated with the pH of soil. In addition, mean temperature and minimum temperature from 80 to 100 days after planting most significantly affected on the accumulation of anthocyanin in color-fleshed potato tubers, that is, higher content of anthocyanin was observed in tubers produced in locations with lower mean temperature and minimum temperature from 80 to 100 days after planting. This information can be useful to producers and industries in selection of proper fields for the production of color-fleshed potato tubers having high quality in Korea.

주성분 분석 및 군집 분석을 이용한 자생국화의 휘발성 향기성분 분류

김수정,하태정,김종윤,유동림,서종택,김율호,홍수영,남정환,손황배,장동칠,김기선 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

본 연구는 자생국화 15분류군의 휘발성 향기성분을 다변량 분석을 통해 분류학적 유연관계를 구명하여 그 분류 기준을 제시하고 조경용 및 약용 소재로 이용하기 위한 기초자료로 활용하고자 본 연구를 수행하였다. 국화속 15분류군의 잎을 대상으로 GC/MS 분석 결과, 총 45종의 휘발성 향기성분이 함유된 것으로 나타났으며, camphor, borneol, phytol, α-pinene, camphene, 1,8-cineole 및 germacrene-D 는 모든 분류군에 공통적으로 함유된 것으로 나타났다. 국화속 15분류군은 hydrocarbon류(sabinene, cymene, β-selinene), alcohol류(1-octen-3-ol, cischrysanthenol,hinesol), ketone류(chrysanthenone, camphor), 및 ester류(cis-sabiene hydrate, trans-chrysanthenyl acetate)의 휘발성 향기성분을 바탕으로 주성분 분석과 군집 분석 결과 총 3그룹으로 분류할 수 있었다. Group I은 5분류군의 구절초류를 나타내었으며, 주요 성분은 D-limonene와 m-thymol이었다. Group II는 낙동구절초, 남구절초, 넓은잎구절초, 서흥넓은잎구절초 그리고 마키노 국화로 구성되었으며, linalool와 cis-chrysanthenol, eugenol, 및 chrysanthenone이 주요 성분이었다. Group III은 감국, 흰감국, 가는잎감국, 산국, 키큰산국이었다. 특히, Group I과 II에서는 α-terpineol이 공통적으로 함유되었으나, Group III에서는 검출되지 않았다.

경관작물 구절초의 고랭지 경사지 밭 토양유실 경감 효과

김수정,손황배,홍수영,김태영,이정태,남정환,장동칠,서종택,김율호 한국자원식물학회 2020 한국자원식물학회지 Vol.33 No.1

There is high vulnerability of soil loss in sloping and highland used for agricultural production due to the low surface covering in summer rainy season. This study evaluated the surface-covering rate of landscape crop in reducing soil loss in the highland. The experiment was conducted in a 55% sloped lysimeter with three treatments of planting density using Korean native chrysanthemum, and investigated the soil coverage rate, run-off water, and soil erosion. The three treatments according to the degree of soil covering are bare soil as the control treatment TC, coverage rate of 43-59% for treatment T1, and, coverage rate of 63-81% for treatment T1, and T2. During the cultivation period, the average reduction of run-off water was 71% for treatment T1 and 76% for treatment T2, which are better, compared with the control. The reduction in eroded soil was 84% in treatment T1 and 98% for treatment T2, which is also better than the control treatment. Therefore, it is possible to alleviate the soil loss in sloping lands by planting chrysanthemum, which is superior among the perennial plant species and considered as a crop with economic value. 고랭지 농업(해발고도 400 m이상)은 주로 해발고도가 높은산지의 경사지에서 이루어지고 있고, 대부분 작물 재배기간이5월부터 9월까지(5개월)로 짧아, 나머지 7개월은 토양 피복이이루어지지 않은 상태로 있어 토양유실 가능성이 높다. 이러한문제점을 개선하기 위해 본 연구에서 고랭지 경사도 55% 라이시미터(Lysimeter)에서 경관성이 높은 구절초를 식재하여 토양유실 저감 효과를 규명하였다. 관행구로 나지(Control, TC) 대비 식재 밀도에 따라 구절초 적은 그룹(T1, 40주), 구절초 많은그룹(T2, 70주)로 하여 총 3처리를 두었다. 구절초의 재배기간(6-10월) 피복율을 조사한 결과 대조구인 나지상태인 TC는 0% 의 피복율인데 반해 구절초 식재한 T1 처리구는 43-59%의 피복율을 보였으며, T2 처리구는 63-81%로 경사지 토양을 피복시키는 효과가 가장 좋았다. 재배기간 평균기온의 5개월 평균은평균기온범위는 16.1℃로 나타났으며 강우량은 1207.9 ㎜로 나타났다. 재배기간 동안 평균적인 지표유출량 경감 효과는 TC 처리 대비 구절초 피복처리구인 T1 처리구는 71%, T2처리구는76%로 우수하였다. 또한, 토양유실량의 경우 TC보다 T1처리구의 경우 84%, 재색밀도가 높은 T2 처리구의 경우 98%의 토양유실 감소효과를 보였다. 따라서, 고랭지 경사지에 영년생 자원식물 중 경관성이 뛰어난 구절초를 식재함으로서 경사지 토양유실을 경감시킬 수 있고, 부가가치가 높은 고소득작물로 활용 가능할 것으로 기대된다.

감자 24품종의 농업적 형질의 유전형과 재배환경의 상호작용 효과

김수정,손황배,박민우,장동칠,홍수영,권오근,정진철,구본철,김율호 한국육종학회 2018 한국육종학회지 Vol.50 No.2

The 24 potato cultivars grown in three regions with different cultivation environments were investigated genotype and environment interaction (GEI) using the Additive Main Effects and Multiplicative Interaction (AMMI), and Genotype main effect and genotype by environment interaction (GGE) biplot model analysis. Potato yield was highly related to environment and GEI factors but the number of tubers and dry matter content were related to the genotype factor. Potato yield was the highest in 'Seohong' in Gangneung, 'Dejima' in Jinbu area, and 'Saebong' in Daegwallyeong area. As a result of analysis of GEI effect between genotypes and environment, 'Seohong' in yield, 'Daejima in tuber, and 'Goun' in dry matter content were superior and were stable. The ‘Seohong’, which has the highest yield and stability, showed promising results and can substitute the ‘Superior’ variety. The 'Goun' was selected as a chip potato processing grade to replace 'Dejima', which currently is the major processing grade. Therefore, as a result of analysis of AMMI and GGE biplot models of agricultural traits of potatoes showed that the cultivation environment has greatest influence on the yield. Therefore, proper site selection for the desired cultivar is necessary for best results. Though it is also important to select the best genotype with a high dry matter content.

일반메밀과 쓴메밀 종실 추출물의 RAW 264.7 대식세포에서 LPS에 의해 유도되는 iNOS 및 염증성 사이토카인 발현 저해를 통한 항염증 효과 비교

김수정,손황배,이경태,신지선,김수연,김수연,남정환,홍수영,서종택,장동칠,김율호 한국식품과학회 2019 한국식품과학회지 Vol.51 No.6

The ethanolic seed extracts of the common buckwheat (CB) and tartary buckwheat (TB) were examined for their anti-oxidant and anti-inflammatory effects on lipopolysaccharide (LPS)-induced RAW 264.7 cells. In this study, it was observed that the rutin content of TB extracts was 65-78 times higher than the CB extracts, while quercetin was only detected in the TB extracts. In addition, TB extracts were observed to have 1.8-2.0 times higher flavonoid and polyphenolic content than the CB extracts. Cytotoxicity was not observed when both the buckwheat extracts were evaluated at concentrations in the range of 6.25-400 μg/mL. The treatment with TB extracts significantly suppressed the LPS-induced nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression at the protein and mRNA levels. The TB extracts more potently inhibited the LPS-induced production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 than the CB extracts. The mRNA levels of TNF-α, IL-1β, and IL-6 were also significantly inhibited both by the TB and CB extracts in a pattern similar to their production.