김치저장성 향상을 위한 동결방법이 배추조직의 물성변화에 미치는 영향

양진한,박소희,유진현,임호수,조재선,황성연 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.2

김치를 10˚C에서 8일간 숙성시켜 산도 0.6~0.7%로 숙성된 김치를 -70˚C와 -20˚C로 냉동하며 -20˚C에서 저장하면서 배추 조직의 elasticity, hardness, 세포 조직의 변화, 드립양을 실험한 결과는 다음과 같다. -70˚C에서 급속 동결한 것과 -20˚C에서 완만 동결한 냉동 김치 중 배추조직의 elasticity는 냉동 저장 15일까지 감소하다 일정하게 유지되었고 hardness는 거의 변화가 없었으며 냉동 방법에 따른 변화도 거의 나타나지 않았다. 투과 전자현미경으로 관찰한 결과 control의 경우 세포벽이 매우 두꺼우며 세포의 모양들이 잘 보존되어 잇는 것을 볼 수 있었고 -20˚C로 냉동 처리하여 해동시킨 세포벽들은 많이 손상되어 있음을 볼 수 있었으며 -70˚C로 급속 냉동시료의 경우 세포벽의 손상 정도가 -20˚C로 냉동 처리한 시료보다 덜 파괴되어 있음을 볼 수 있었다.한편 냉동 저장기간 동안 드립의 손실량의 변화는 -70˚C로 냉동 처리한 시료의 드립양은 3~4%정도로 -20˚C로 냉동 처리한 시료의 5~6%에 비해 적은 것을 알 수 있었다. This study was conducted to investigate the effect of freezing methods, show freezing at -20℃ and rapid freezing at -70℃, on physical properties of Chinese cabbage in frozen Kimchi during storage at -20℃. Elasticity of midrib of Chinese cabbage in frozen Kimchi was decreased until 15 days and did not changed thereafter during storage at -20℃, Hardness of that was not changed during storage. Those results of elasticity and hardness of slow frozen sample are similar to rapid frozen sample. By the morphological observation through transmission electron microscope, more of cellular structure of Chinese cabbage in slow frozen was destructed than that of rapid frozen sample. Drip loss was more in slow frozen sample than that in rapid one.

LP강판의 두께변화에 따른 강거더의 효율성 평가

양경환,정경섭,이종선,권소진 충북대학교 건설기술연구소 2003 建設技術論文集 Vol.22 No.1

There has been an increased interest in the Longitudinally Profiled(LP) plate as a structural element for bridges, due to its advantage to efficiently distribute the sectional force applied to the structure. The advantage also includes the reduction of weight and the number of welding points. The LP plate was originally developed in Europe, and the application has since increased rapidly on steel bridges in Europe and Japan. Though widely used internationally, much of the study for the structural application of the LP plate is yet to be performed. In this paper, the workability of the LP plate was evaluated by the tests for tensile and bending. In addition, the stability was reviewed and verified by a comparison between experimental and analytical results. The economical efficiency was also examined for different section types by finite element analysis.

항우울제 장기 투여에 의한 흰쥐 해마에서 PhosphoCREB의 발현 증가

김소양,한진희,이창욱,백인호 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.5

목 적 : 저자들은 paroxetine(selective serotonin reuptake inhibitor). desipramine(selective norepinephrine reuptake inhibitor), moclobemide(reversible monoamine oxidase inhibitor)의 항우울제를 흰쥐에 장기 투여한 후 해마에서 phosphoCREB의 발현정도를 측정하고 이를 항정신병약물, 항불안제 및 control을 장기 투여하였을 때의 발현정도와 비교해 보고자 하였다. 방 법 : 실험동물로는 체중 200∼300g의 Sprague-Dawley 수컷 흰쥐 240마리를 사용하였고, paroxetine 투여군, desipramine 투여군, moclobemide 투여군, haloperidol 투여군, lorazepam 투여군과 대조군으로 나누고 각각의 약물을 1일간, 3일간, 7일간, 14일간 각각 하루 한차례씩 복강내 주입을 하였다. 흰쥐는 마지막 투약 15분후에 대뇌를 적출하였고 면역조직화학법을 이용하여 해마에서 발현되는 phosphoCREB(+)신경원 수를 측정하였다. 결 과 : 항우울제인moclobemide 투여군에서 3일째부터 phosphoCREB의 발현을 증가시켜 7일째와 14일째 모두 증가되었고 paroxetine 투여군과 desipramine 투여군도 7일째와 14일째 증가를 나타내었다. 그러나 항우울제가 아닌 약물을 투여한 군(lorazepam 투여군과 haloperidol 투여군)에서는 대조군과 비교하였을 때 투여 기간에 따른 phosphoCREB의 증가는 없었다. 결 론 : 다양한 항우울제의 장기 투여는 항정신병약물과 항불안제의 장기 투여와 비교하면 모두 해마에서 phosphoCREB의 발현을 증가시킴을 알 수 있었다. 따라서 이는 다른 약제와 구별되는 항우울제만의 특이한 공통적 약물작용기전으로 생각된다. Objectives : This study was designed to assess the expression of phosphoCREB in rat hippocampus after chronic administration of various antidepressants in comparison with chronic administration of antipsychotic and antianxiety drugs. Methods : Male Sprague-Dawley rats(200-300g) were used for this experiment. The subjects were divided into 6 groups according to specific treatment agents(paroxetine, desipramine, moclobemide, haloperidol, lorazepam, vehicle) which were administered daily for 1day, 3day, 7day, and 14days by intraperitoneal injection respectively. Brains were removed 15 minutes after the last treatment. PhosphoCREB immunoreactivity was measured by phosphoCREB(+) cell counts in hippocampus of rats. Results : Expression of phosphoCREB was significantly increased from day 3 in moclobemide group, from day 7 in paroxetine and desipramine groups, and increased most significantly from day 14 in all antidepressant-administered groups, with no increase in other two groups(lorazepam and haloperidol groups) throughout the experiment and even after 14 days of treatment. Conclusion : These result suggest that increased expression of phosphoCREB after chronic administration of antidepressants, not of antipsychotic or antianxiety drugs, demonstrates pharmacolgical specificity of antidepressant treatment in rat hippocampus regardless of their receptor preference.

Vibrio vulnificus 박테리오 파아지의 특성

오양효,윤소겸,공은진,김민정,김영부,박영민 대한감염학회 1995 감염 Vol.27 No.5

목 적 : 이미 보고되어 있는 파아지들의 경우, 일반적인 형태, 숙주역과 몇 가지의 성질만 연구되어 있으므로 보다 상세한 특성에 대한 연구가 필요한 상태이다. 본 연구에서는 해양으로부터 분리한 V. vulnificus를 숙주균으로 하여 투명한 용균반을 형성하는 파아지를 분리해 낸 다음, 분리된 V. vulnificus 파아지의 특징을 연구하고자하였다. 방 법 : V. vulnificus 를 숙주균으로 하여 해산물로부터, 숙주균에 특이적인 파아지를 분리하고, 농축 정제과정을 거쳐 파이지입자를 순수분리하였다. 파아지의 형태를 전자현미경으로 관찰하였으며, 분리된 파아지의 숙주특이성을 조사하고, 파아지 입자의 물리적 특성을 검토하기 위해, 온도 안정성시험, pH 안정성 시험을 실시하였다. 파아지의 흡착율, 파아지의 흡착율에 미치는 무기염 이온의 영향, 온도의 영향과 pH의 영향을 관찰하였다. 파이지의 일단증식곡선을 구하였으며 토끼 면역과 교차 중화 시험, 파아지 핵산의 특성, 구조단백질의 특성을 조사하였다. 결 과 : 전자현미경관찰결과 본 연구에서 분리한 파아지는 두부와 미부를 가지는 형태로 미부에 꼬리판과 꼬리섬유를 가지고 있었다. 파아지의 물리적성질시험 결과 파아지는 50℃이하의 온도에서 안정하였으며 중성pH영역에서 안정하고 산성과 알칼리 영역에서는 활성을 잃었으며 자외선에는 35초 조사에 50% 정도 불활성화 되었다. 파아지는 약 65%의 흡착율을 나타내었으며 흡착에 ??, ??을 요구하였다. 파아지입자가 안정한 온도와 pH영역에서는 흡착에 영향을 받지 않았으며 일단증식시험 결과 108 PHU/ml의 파아지 입자를 생산하였다. 정제한 파아지를 항원으로 하여 토끼에 면역한 항혈청에 파아지가 중화되었으며 파아지 핵산은 이중나선 DNA였다. 파아지의 구조단백질은 SDS-PAGE polyacrylamide gel 전기영동 결과 2개의 밴드를 나타내었다. 그리고 Western blotting 결과 파아지의 전기영동 결과와 동일한 위치의 밴드에서 항원성을 나타내었다. 결 론 : 인체병원균으로 중요성이 인식되고 있는 V. vulnificus는 해산물의 위생과 국민건강에 직접적인 영향을 미칠 수 있는 세균으로 이에 대한 활발한 연구가 요구되는 실정이다. 파아지의 분리율은 숙주균의 분리율에 의존하므로 숙주균의 해수내 분포에 영향을 미친다고 추측되며, 파아지는 숙주균에 감염되어 숙주균을 용균시키므로 숙주균의 개체수를 조절하여 생태학적 측면에서 중요한 기능을 담당하는 것으로 여겨진다. Background : Vibrio vulnificus causes severe wound infection and life-threatening septicemia. In this study we present the characteristics of V. vulnificus bacteriophage and the basis of pathogenicity study of V. vulnificus related on bacteriophage. Methods : We isolated bacteriophage for V.vulnificus from marine products and examined its physiological and biochemical characteristics, and the ultrastructure by electron microscopy (TEM,SEM). And also, we investigated host ranges, temperature stability, pH stability, inactivation by UV irradiation, DNA isolation and analysis and structural protein analysis of the bacteriophage. Results : The adsorption rate of bacteriophage was above 80% and had a little influence at 10-45℃ and pH5-10. Isolated bacteriophage was used to produce antiserum against rabbits and its antigenicity was investigated by cross-neutralization. Western blot analysis showed the capsid protein of bacteriophage had antigenicity. The bacteriophage had double-strand DNA. In restriction analysis, the phage DNA was digested HinfI. The structural protein of phage showed two bands of 34.7kDa and 18.4kDa on SDS-PAGE. Conclusion : The specificity of bacteriophage may be due to the specific binding site that be possessed by host strain surface. Therefore, it seems that virulent bacteriophage controls the number of host strain and then takes a role on natural marine environment.

Calcium phosphate 결정 성장 최적화를 위한 Ti 표면의 BaTiO_3 박막 형성에 관한 연구

조진우,양홍서,박영준,황규석,송종은,이용렬 전남대학교 치과대학 2001 구강과학 Vol.13 No.2

This study was purposed to establish an optimized manufacturing process for a negatively charged ferroelectric thin flim on titanium substrate in order to develop a new implant system having the mechanical strength and bone forming ability. Ferroelectric materials show an alignment of positive and negative charges by poling treatment. Based on this property, this study was performed with the assumption that the Ca^2+ ions would be easily attracted on negatively charged surface and the attracted cation might behave as nuclei for bone-like crystal grownth in biological solutions. In order to have an osteoinducibility by the ferroelectric thin film coating, continuous and flawless crystalline thin film should be formed with ferroelectricity, and finally should be adequately poling treated. In this study, BaTiO_3 (BTO) was selected as a ferroelectric material. Before thin film coating process, most favorable poling condition was investiged with the evaluation of the difference in calcium phosphate (Ca-P) formation in simulated body fluid (SBF). Moreover, the parameters to form an optimum thin film were studied. Dense bulk BTO disks were fabricate using cold isostatic pressing(CIP) and sintered in air at 1300℃ for 2 hours. Sintered BTO disk was polished, and he crystallinity, microstructure, and ferroelectric characteristics were evaluated using an X-ray diffraction analysis (XRD), a field emission scanning electron microscope (FE-SEM), and an Impedance analyzer. To find the optimum poling condition for the calcium phosphate crystal formation, sintered bulk BTO specimens were treated with either of following poling conditions; 1) room temperature poling treatment [ polarizing field (Ep) = 25kV/cm, at 25℃ for 2 hours], 2) under Tc medium-temperaturepoling treatment (Ep=20kV/cm, from 85˚C to 25˚C for 2 hour), and 3) above Tc high-temperature poling were immesed in simulated body fluid (SBF) for 30 days, and the formation of calcium phosphate (Ca-P) layer n the surface was evaluated. The crystallinity, microstructure, and ferroelectric characteristic of BTO thin film were evaluated withthe variation of final heat treatment. It was performed after establishing the starting substance-solvent mixing ratio, coating times, and pre-heating temperature optimal to form continuous and flawless BTO thin film adequate for having ferroelectricity. Perparation of BTO thin film was made by dipping-pyrolysis technique using metal naphthenates as starting substances. BTO thin films was spin-coated on Ti substrate by dipping-pyrolysis technique using sol obtained by mixing Ba-naphthenate and Ti-naphthenate as starting substances. Pre-firing temperature of BTO thin film was performed at 450℃ because organic-solvent ws completely volatilized without any crystal growth, and the final heat treatment was performed at 600, 700 or 800℃, respectively. The results are as follows; 1. BTO disk sintered at 1300℃ for 2 hours showed average grain size of about 1㎛ and the relative dielectric constant at 1㎑ was abut 3000∼3500. 2. After immersing the sintered bulk BTO poled at respective conditions in SBF for 30 days, Ca-P layers were present on the negatively charged surfaces. In contrary, positively charged Bto surface did not show any noticeable charge of the surface microstructure after SBF immersion. 3. In he case of poling condition 1 and 2 treated with below Tc, Ca/P ratio showed a relatively low value of 1,.2∼1.5. While, in the case of poling condition 3 treated with above Tc, Ca/P ratio was 1.5∼1.67, which is similar to that of biological apatite. This phenomenon demonstrates that poling the BTO above the temperature of Tc is preferable for the Ca-P formation. 4. For thin film BTO coastin, the starting substance was prepared by an equimolar mixing of the Ba and Ti-naphthenate. Dilution of the starting substance with toluene was most appropriate at sol : toluene ratio of 5.2:4.8 Too thin sol produced porosity in film, and too thick sol resulted cracks in the film. By repeating the coating and pre-firing procedure 15 times, homogeneous film of 0.5㎛ thickness could be fabricated, and perfomed well during the poling treatment without breakdown. 5. The heat-treatmen of the BTO thin film at 700℃ produced a homogeneous crystalline structure without heterogeneous TiO2 formation. The BTO thin film heat-treated at 600℃ showed non-crystallinity and no ferroelectricity. Treatment at 800℃ produced heterogeneous TiO2 phase in the film, which is unfavorabe for having ferroelectricity by the distortion of the perovskite structure. The relative dielectic constant at 1 ㎑ was 152 and 112 by heat-treatment at 700 and 800℃, respectively. In summary, these results demonstrated that poling the ferroelectric B_aT_iO_3 surface negatively is effective for the formation of Ca-P layer in simulated body fluids. Poling condition at a temperature cooling from above Tc down to room temperature is most effective. By optimizing the method of BTO thin film coating on metal substrate for the formation of Ca-P layer, the data of this study might be applied for the manufacture of new bioactive implant system.

유산균들의 콜레스테를 저하성, 내산성, 내담즙성, 항생제 내성 비교

박소영,고영태,정후길,양진오,정현서,김영배,지근억 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

기능성 요구르트의 제조에 사용되는 유산균은 인체에 유익한 생리활성과 우수한 생존능력을 보유하고 있는 것이 바람직하다. 본 연구에서는 다양한 유산균주의 산과 담즙 및 항생제에 대한 내성을 조사하였으며 in vitro에서 콜레스테롤 저하능을 조사하였다. In vitro에서 콜레스테롤 저하능이 우수한 13균주를 선발하여 이들의 동결건조 분말을 고 콜레스테롤 식이의 실험쥐에 투여하였다. 그 결과 8균주는 비 투여구보다 유의적으로 18.3~27.3%의 콜레스테롤 저하능을 나타냈다(P≤0.05). 이들 중에서 Bifidobacterium infantis AM-220, Lactobacillus AM-245, Streptococcus MA-1의 3균주를 이용하여 요구르트를 제조한 후 동결건조 분말을 이용하여 급여실험을 수행한 결과, 총 콜레스테롤과 LDL 함량이 유의적으로 감소하는 것으로 나타났다. For a probiotic yoghurt it is desirable to utilize lactic acid bacteria with a high survival rate and beneficial function to human beings. We have examined a variety of lactic acid bacteria to assess the acid and bile tolerance and antibiotic resistance. In addition, an in vitro culture experiment was performed to evaluate their ability to reduce cholesterol levels in the growth medium. Thirteen strains were selected from in vitro cholesterol assays and fed to Sprague-Dawley rats with a high-cholesterol diet. Among the 13 strains tested, 8 strains were shown to reduce serum cholesterol levels significantly after 24 days of administration in vivo. Rats were fed lyophilized yoghurt powder fermented with a combination of 3 selected strains: Bifidobacterium infantis AM-220, Lactobacillus AM-245, and Streptococcus MA-1. The levels of total cholesterol and low density lipoprotein were significantly lower (p≤0.05) in rats fed the yoghurt powder compared with control group. These studies suggest that yoghurt fermented with appropriately selected lactic acid bacteria may have a anticholesterolemic effect.

압궤손상을 입은 혈관에서 Ginkgo Biloba 추출물(EGb 761)이 혈관문합술후 개통율에 미치는 영향

강소라,이동진,차진한,김양우 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

Replantation after crush amputation has a relatively low success rate. Thrombus formation due to vessel and tissue trauma is considered as the principal cause of failure. In the laboratory and clinically, we have been tried to improve the post-anastomosis patency rate in crushed microvasculature. To accomplish this, we have usually used several anticoagulant drugs. Extracts from the leaves of Ginkgo biloba (EGb) have been used therapeutically for centuries. EGb exerts a number of pharmacologic actions. Eighty rats were control group and another 80 rats were treated with EGb 761. The femoral arteries underwent crush injury with an energy of 0.4J, and the vessles in each group were divided and anastomosed by a standard microsurgical technique. Each group was comprised as follows: ① control group(A1,A2): group A1(n=40); intraluminal saline irrigation, group A2(n=40); intraluminal saline irrigation+heparin 40 u/ml(IV) ② EGb 761 treated group(B1, B2): groupB1(n=40); intraluminal saline irrigation, group B2(n=40); intraluminal saline irrigation + heparin 40 u/ml(IV). At postoperative 14 days, the patency rates were; group A1 20%, group A2 77.5%, group B1 47.5%, group B2 92.5%. These results were interpreted as follows: the patency rate was significantly increased in the EGb 761-only treated group(p<0.01), the heparin-only treated group(p<0.01), and the EGb 761 and heparin-combined at crushed microvessel surgery. However the patency rate of the EGb-only treated group was significantly lower than that of the heparin-only treated group(p<0.01). And in the EGb 761 and heparin-combined treated group compared to the heparin-only treated group, there was some patency rate increase in the combined treated group, but there was no significant difference between them(p=0.060).

컴퓨터 작업 시 의자 등받이 위치가 근육 활성도에 미치는 영향

김민우,변승진,이경은,정소영,조주영,최원자,최찬양,김경 대구대학교 특수교육재활과학연구소 2011 再活科學硏究 Vol.29 No.1

이 연구는 컴퓨터 작업시 의자 등받이 위치가 근육활성도에 미치는 영향을 알아보기 위해 36명의 근골격에 문제가 없는 성인 남녀가 참가하였으며 대상자들은 등받이가 뒤에 있는 의자와 등받이가 없는 의자, 등받이가 앞에 있는 우리들 의자를 무작위로 배정하고 동일한 컴퓨터 작업을 실시하게 하였다. 실험 중, 표면근전도기를 사용하여 의자에 따른 위등세모근, 머리널판근 그리고 척주세움근의 근활성도 변화를 측정하였다. 이 연구의 결과를 종합해보면 등받이가 앞에 있는 의자는 허리 근육의 부담을 줄이지만 목근육에 부담이 늘어나고 등받이가 뒤에 있는 의자와 없는 의자는 허리근육에는 다소 무리가 가지만 목근육에는 부담이 덜하다는 것을 알 수 있었다. 이러한 결과로 환자의 증상에 다라 컴퓨터 작업을 하는 동안 의자 등받이의 위치를 조절하여 치료에 도움이 될 수 있다고 생각되어진다. Objective : In this study, we checked and observed people's change of body muscle and muscle activity who do computer work on different chairs. We set three kind of chairs which one is a stool and another one is the back is fixed, and the other one is the back is fixed in front of chair. Subject : Accounting for 36 health men and women, we divided these people to 3 groups and put them on the three cases(position 1, position 2, position 3). We measured muscle activity of upper trapezius, Splenius Cervics, Erector Spinae from the groups by using surface electromyogram system. Methods : The standard of the electromyogram was 'reference voluntary contraction', and we carried out 'one way anova' to compare muscle activity of three groups. Results : In position 1, reference voluntary contraction of upper trapezius muscle activity was 123.2821%, Splenius Cervics was 141.7526%, and Erector Spinae was 254.5233%. In position 2, reference voluntary contraction of upper trapezius muscle activity was 132.9395%, Splenius Cervics was 141.7526%, and Erector Spinae was 246.6540%. In position 3, reference voluntary contraction of upper trapezius muscle activity was 190.6487%, Splenius Cervics was 270.2332%, and Erector Spinae was 182.1021%. Both upper trapezius and Splenius Cervics muscle activity of position 3 group was higher than the others groups. In position 1 group, Erector Spinae muscle activity was hight than the other groups. And either was position 2 group's(p<0.05). Conclusion : Therefore the chair which is the back is fixed in front is more comfortable for waist muscle but it's not good for neck. And the other chairs are better in waist muscle but not in neck muscle. So far, when we do a computer work, it's prefer to sit on the group 1&2's chairs to reduce neck muscle's stress and sit on group 3's chair to reduce waist muscle's stress.

임상검체에서 분리된 그람 음성 세균들에 대한 Isepamicin의 시험관내 항균력

권현희,박수진,소민욱,박현구,최성호,김미나,최상호,정진용,우준희,김양수 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

Background : The increasing incidence of multidrug-resistant gram-negative bacteria causing nosocomial infections is an important clinical problem. Isepamicin is a recently developed aminoglycoside which has been known to have potent activity against gram-negative organisms. We evaluated the in vitro activities of isepamicin and other aminoglycosides against a large number of gram-negative organisms. Materials and Methods : We tested the in vitro antimicrobial activities of isepamicin, amikacin, gentamicin, and tobramycin against 566 gram-negative organisms collected between January 2006 and June 2006 in Asan Medical Center. Minimal inhibitory concentrations (MICs) were determined and interpreted according to the recommendations of Clinical and Laboratory Standard Institute (CLSI). The breakpoint MIC used for interpretation of isepamicin was MIC≤16 ㎍/mL as susceptible, 32 ㎍/mL as intermediate, and 64 ㎍/mL as resistant. Results : The MIC_(50)/MIC_(90) of isepamicln for Escherichia coli, Klebsiella pneumon/ae, Pseudomonas aeruginosa, Acinetobacter baumannii, and Enterobacter cloacae were 1/2, 0.5/>128, 4/16, 16/>128, and 1/2 ㎍/mL, respectively. The susceptibilities for E. coli, K. pneumoniae, P. aeruginosa, A. baumannii, and E. cloacae were 100%, 86.4%, 89.7%, 50.0%, and 96.6%, respectively. For E. coli, K. pneumoniae, P. aeruginosa, and E. cloacae, isepamicln had better in vitro activities than gentamicin and tobromycin, and had similar activities with amlkacin. For A. baumanii, all four tested aminoglycosides had similar in vitro activities. Conclusion : Isepamicin had excellent in vitro activities against gram-negative organisms, except A. baumanii. The overall in vitro activities were similar with amikacin. 목적 : 그람 음성 세균은 다양한 지역사회 및 병원 획득 감염의 원인균이다. 최근에는 병원 감염을 일으키는 그람 음성 세균에서 항균제 내성균의 출현과 확산이 큰 문제가 되고 있다. Isepamicin은 최근에 소개된 gentamicin B의 유도체로 외국에서의 연구에 의하면 그람 음성 세균들에 대한 항균력이 우수함이 보고 된 바 있다. 본 연구에서는 우리나라의 임상 균주들을 대상으로 그람 음성세균에서 isepamicin의 시험관내 항균력을 기존의 아미노배당체 제재들과 비교하여 평가하고자 하였다. 재료 및 방법 : 2006년 1월에서 6월 사이에 서울아산병원 환자에서 분리된 Escherichia coli 113 균주, Klebsiella pneumoniae 110 균주, Pseudomonas aeruginosa 117 균주, Acinetobacter baumannii 108 균주, 그리고 Enterobacter cloacae 118 균주를 대상으로 isepaimcin, amikacin, gentamicin, tobramycin에 대한 최소억제농도(minimal inhibitory concentration, MIC)를 한천 희석법(Mueller-Hinton Agar)을 이용하여 측정하였다. 감수성여부는 CLSI 기준에 따랐으며, isepamicin은 MIC≤16㎍/mL를 감수성, MIC=32 ㎍/mL를 중등도 내성, MIC≥64 ㎍/mL를 내성으로 판정하였다. 결과 : Isepamicm의 E. coli, K. pneumoniae, P. aeruginosa, A baumannii, E. cloacae에 대한 MIC_(50)/MIC_(90)은 각각 1/2, 0.5/>128, 4/16, 16/>128, 1/2 ㎍/mL였고, 항균제 감수성률은 차례대로 100%, 86.4%, 89.7%, 50.0%, 96.6%였다. 기존 아미노배당체 제재들과 비교하면 E. coli, K. pneumoniae, P. aeruginosa, E. cloacae에서는 amikacin과 비슷한 감수성률을 보였고, gentamicin과 tobramycin보다 높은 감수성률을 보였다. A baumannii의 경우 isepamicin의 감수성률이 50.0%로 비교적 낮았지만 amikacin, gentamicin, tobramycin도 각각 50.0%, 46.3%, 47.2%의 감수성률을 나타내어 4가지 항균제가 모두 비슷한 항균력을 보였다. 결론 : Isepamicin은 그람 음성 세균들에 있어 기존의 아미노배당체 제재들과 비교했을 때 amikacin과는 동등한 시험관내 항균력을 보이고 gentamicin, tobramycin보다 뛰어난 시험관내 항균력을 보여 사용이 유망한 아미노배당체 중 하나라 생각된다.

Creutzfeldt-Jacob병 1례

홍승철,한진희,정종현,김소양,허균,이성필 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

저자들은 Creutzfeldt-Jacob병 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. 환자는 약 4개월에 걸친 비교적 짧은 기간 동안 중증의 인지 기능 장애와 시각 장애, 보행장애와 하지의 약화, 불수의적 운동 등과 함께 섬망상태에까지 이르게 되어 본원 정신과에 입원하게 되었다. 정신상태 검사상, 반응시간의 지체, 언어장애와 보속증, 시간에 대한 지남력 장애, 주의 집중력 장애, 단기 및 장기 기억 장애, 추상적 사고능력 장애 등의 소견을 보였고, 이러한 증상들은 입원후 치료에도 불구하고 빠르게 악화되었다. 뇌파검사상 지속적인 다발초점성 서파가 모든 전극에서, 특히 우측 전두부에서 현저하게 나타나는 소견을 보였고, 병리조직학적 검사상 대뇌 피질에 신경손실을 동반한 해면화와 신경교 증식증을 나타냈다. 이상의 소견들은 모두 CJD의 진단에 합당하였다. We report a case of Creutzfeldt-Jacob disease and review the relevant literatures. This 56-year-old male patient was admitted to our hospital with a history of rapidly progressing cognitive dysfunction, visual disturbance, gait disturbance, weakness, involuntary movement of lower extremities, and the symptoms of delirium. On mental status examination, he showed psychomotor retardation, speech disturbance, perseveration, disorientation, impairment of short-term and long-term memory, and inability of abstract thinking. He had a rapid downhill course, along with shakiness of trunk, rigidity of the limbs, myoclonus, confusion, and finally, inability to speak or move on the bedside. On electroencephalogram(EEG), continuous multifocal show delta activities were found in all leads, especially in the right frontal areas. Brain biopsy showed, spongiosis with neuronal loss and gliosis noted at the cerebral cortex. These findings were compatible with Creutzfeldt-Jacob disease.