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Primary and secondary structure of the 25 S rRNA from the Dimorphic Fungus Mucor racemosus
지근억 한국농화학회 1990 Applied Biological Chemistry (Appl Biol Chem) Vol.33 No.2
A 9.76-Kb ribosomal DNA repeat unit from the nuclear genome of the dimorphic fungus Mucor racemosus (zygomycetes) was cloned into E. call plasmids as four overlapping pieces and mapped. The nucleotide sequence of the complete 25s rRNA gene and flanking regions were determined. The 5' and 3' ends of the structural gene was identified by comparison with the published sequence for the S. cerevisiae gene. The Mucor gene was found to posses 3469 by compared with 3392 by in the Saccharomyces gene. The main reason for this difference is the apparent insertion of a 106-by segment(from bp-608 to bp-713) in the Mucor gene. This 106-by segment having a GC content of only 37%, departs radically from the typical GC-rich insertion sequences found in the gene of higher eukaryotes. Indeed the entire 25S rRNA gene from Mucor has a very low content (42.8%) compared with homologous gene from other eukaryotes. Saccharomyces and Drosophila were the only other organisms for which this value has been reported below 50%. The Saccharomyces 25S rRNA molecule is post-transcriptionally methylated at 43 sites, 30 of which have been identified in the base sequence by Veldman et al.. Our sequence data indicated that all 30 sites were conserved in Mucor gene. Moreover, 750 bp immediately surrounding the 30 methylation sites showed 98% sequence similarity with the gene from Saccharomyces. By means of computer-assisted modeling, we were able to organize the 25S rRNA from Mucor into seven domains that are clearly homologous with the established domains in the molecules from E. coli and Saccharomyces. The stem and loop structure are generally similar, though nor always identical to the E. coli-Saccharomyces paradigm. The inserted 106bp segment is found in domain II as part of a highly stable stem-loop structure. Domain VII contains a loop with a base sequence identical to the alpha-sarcin cleavage site located in homologous regions of both E. coli 23S rRNA and Saccharomyces 25S rRNA. Even those domains (II and VII) in which sequence similarity to that of Saccharomyces was its lowest secondary structure was substantially conserved.
한국인의 분변으로부터 Eubacterium을 분리하기 위한 선택 배지 조사
지근억 한국산업미생물학회 1994 한국미생물·생명공학회지 Vol.22 No.4
Eubacterium is one of the predominant bacteria in the human large intestine. Currently ES (Eubacterium Selective) medium developed by T. Mitsuoka is commonly used as a selective medium. Neomycin sulfate which is one of the selective agents of ES medium inhibited about 50% of the growth of Eubacterium isolates, whereas nalidixic acid inhibited only 5% while inhibiting other intestinal bacteria. NES medium which replaced neomycin with nalidixic acid in the ES medium was designed and shown to be better for the isolation and enumeration of Eubacterium sp. than ES medium.
Mucor racemosus 18S rRNA gene의 3'말단 염기해독
지근억,김진경 한국미생물학회 1991 미생물학회지 Vol.29 No.5
the nucleotide sequence of the 3' terminal 568 bases of the 18S rRNA gene from Mucor racemosus was determined. The 3' end of the structural gene was identified by comparison with the published sequence for the Saccharomyces cerevisiae gene. The M. racemosus gene was found to share 83.8% homology with that of S. cerevisiae and 71-81% homology with those of human, mouse, maize, Xenopus laevis and Tetrahymena thermophila. The known methylation sites in X. laevis and human were also highly conserved in M. racemosus and located within most conserved regions of 18S RNA gene throughout evolution.