Identification of Genes Modulated by High Extracellular Calcium in Coculture of Mouse Osteoblasts and Bone Marrow Cells by Oligo Chip Assay

Kim, Hyung-Keun,Song, Mina,Jun, ji-Hae,Woo, Kyung-Mi,Kim, Gwan-Shik,Baek, Jeong-Hwa The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Calcium concentration in the bone resorption lacunae is high and is in the mM concentration range. Both osteoblast and osteoclast have calcium sensing receptor in the cell surface, suggesting the regulatory role of high extracellular calcium in bone merabolism. In vitro, high extracellular calcium stimulated osteoclastogenesis in coculture of mouse osteoblasts and bone marrow cells. Therefore we examined the genes that were commonly regulated by both high extracellular calcium and 1,25(OH)_(2)vitaminD_(3)(VD3) by using mouse oligo 11 K gene chip. In the presence of 10 mM[Ca^(2+)]e or 10 nM VD3, mouse calvarial osteoblasts and bone marrow cells were co-cultured for 4 days when tartrate resistant acid phosphatase-positive multinucleated cells start to appear. Of 11,000 genes examined, the genes commonly regulated both by high extracellular calcium and by VD3 were as follows; 1) the expressions of genes which were osteoclast differentiation markers or were associated with osteoclastogenesis were up-regulated both by high extracellular calcium and by VD3; trap, mmp9, car2, ctsk, ckb, atp6b2, tm7sf4, rab7, 2) several chemokine and chemokine receptor genes such as sdf1, scya2, scyb5, scya6, scya8, scya9, and ccr1 were up-regulated both by high ectracellular calcium and by VD3, 3) the genes such as mmp1b, mmp3 and c3 which possibly stimulate bone resorption by osteoclast, were commonly up-regulated, 4) the gene such as c1q and msr2 which were related with macrophage function, were commonly down-regulated, 5) the genes which possibly stimulate osteoblast differentiation and/or mineralization of extracellular matrix, were commonly down-regulated;slc8a1, admr, plod2, lox, fosb, 6) the genes which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were commonly up-regulated;s100a4, npr3, mme, 7) the genes such as calponin 1 and tgfbi which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were up-regulated by high extracelluar calcium but were down-regulated by VD3. These results suggest that in coculture condition, both high extracellular calcium and VD3 commonly induce osteoclastogenesis but suppress osteoblast differentiation/mineralization by regulating the expression of related genes.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

Protective Effects of N-acetylcystenine and Selenium against Doxorubicin Toxicity in Rats

Yeo-sung Yoon,Sun-don Kim,Min-hye Lee,Heung-shik S.Lee,In-se Lee,Je-kyung Sung,Eun-sung Park 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Effects of N-acetylcysteine and Selenium against Doxorubicin Toxicity in RatsEun-sung Park, Sun-don Kim1, Min-hye Lee, Heung-shik S. Lee, In-se Lee, Je-kyung Sung and Yeo-sung Yoon*

안면비대칭 환자의 natural head position에 대한 정모두부방사선학적 연구

김현,황현식,김경근 전남대학교 치과대학 2000 전남치대논문집 Vol.12 No.1

The purpose of this study was to find the characteristics of the frontal natural head position(NHP) of patients with facial asymmetry, and to contribute to the diagnosis of facial anymmetry in the clinical examination of orthodontic patients. Twenty adult patients who had apparent facial asymmetry and no severe sagittal skeletal discrepancy were selected as the asymmetry group, and 21 young adults who had symmetric faces were selected as the symmetry group. Frontal cephalograms were obtained in the state of NHP using a pivot-mounted fluid level device. The degree of the menton deviation was defined as the angle between the line drawn through crista galli and anterior nasal spine and the line drawn through crista galli and menton. The following angles were measured and each of them was compared with the degree of the menton deviation: one is the angle between the true vertical line and the supra-orbital line which is a tangent line to the extreme cranial point on the supra-orbital margin, and the other is the angle between the true vertical line and the cervical line drawn through the midpoint of atlas and 4th cervical vertebra. Through the statistical analysis, following results were obtained. 1. The angle between the supra-orbital line and the true vertical line was much more deviated from the right angle in the asymmetry group than in the symmetry group. 2. The angle between the cervical line and the true vertical line in the asymmetry group showed greater tendency than in the symmetry group, but the difference was not statistically significant. 3. In the asymmetry group, the degree of the menton deviation was positively correlated with angle between the supra-orbital line and the true vertical line. The above results suggest that facial asymmetry patients show the tendency to have the tilted NHP to compensate the deviation of menton position.

수면 다원검사상 정상인 단순 코골기 환자에서 레이저 수술의 임상적 효과

김경수,이정권,김성식,박국진,한재욱,김원석 대한이비인후과학회 1998 대한이비인후과학회지 두경부외과학 Vol.41 No.3

음향방출 신호처리에 의한 플라즈마용사코팅 피막부식재의 파손해석

김귀식,박경석,홍용의,현창해 제주대학교 산업기술연구소 2001 尖端技術硏究所論文集 Vol.12 No.1

This paper was to investigate of a adhesiveness of the plasma sprayed coating materials did a salt spray by acoustic emission method in tensile loading. The powders used for the coating were nickel aluminum composite powder Ni-4.5wt.%Al and titanium dioxide powder TiO2. These powders were coated on a carbon steel S45C by plasma spray method. The salt solution was a 5% NaCl and the salt spray times were 2, 5 and 10 hours respectively. The salt solution penetrated into the surface of the substrate through pore of the coating layer built in the process of plasma spay. Corrosion productions formed on the surface of substrate. The adhesiveness between the substrate and the coating layer is weaken by corrosion and the exfoliation initiated chiefly at the corrosion surface of the substrate. The AE events and energy of the corroded coating specimens decreased as the salt spray times increased. The AE amplitudes increased at strain range of 6%-11%.

급성백혈병환자에서 분리된 Staphylococcus epidermidis의 분자 역학적 특성 및 위험요인

김경미,이동건,김상일,최정현,한성숙,신완식 대한병원감염관리학회 2003 의료관련감염관리 Vol.8 No.2

자기공명영상으로 진단된 바이러스성 능형뇌염

김재영,서경식 건국대학교 의과학연구소 1994 건국의과학학술지 Vol.4 No.-

Viral neuritis due to herpes simplex type II has been well documented. The virus lies dormant within the gasserian ganglion of the trigeminal verve. Retrograde spread into the brainstem is rare. This has been referred to as rhombencephalitis. The reported cases of rhombencephalitis detected by MR are very rare. So we report a case of viral rhombencephalitis which was diagnosed by MR. A 35 year-old female patient was admitted due to dizziness, nausea, tinnitus(Lt.), hearing impairment(Lt.), and hypoesthesia on the left face, which were similar to the symptoms of acoustic neuroma. MR showed the enhanced high signal intensity in left middle cerebellar peduncle. One month after treatment, the symptoms resolved. Follow-up MR showed the decreased high signal intensity. We report this case with review of the literature.

접형동에서 발생한 후비공 비용

김재영,서경식,허형빈 건국대학교 의과학연구소 1995 건국의과학학술지 Vol.5 No.-

Choanal polyps are a separate clinical entity. While the etiology of choanal polyps is unclear, they present a fairly uniform clinical picture. Maxillary sinus is the usual site of origin, but in rare cases, a choanal polyp may originate from a different site, e.g, turbinate sinus, anteroinferior surface of the ethmoidal bulla, sphenoethmoidal recess, or sphenoid sinus. A 37 year-old woman was referred with 6 months history of nasal obstruction. Anterior rhinoscopic examination revealed the large polyp on the posterior portion of nasal cavity extending to the choana. Endoscopic and radiologic evaluation was taken, and FESS was performed. Polyp was removed completely. We report this case with review of the literature.

혈액종양병동 입원환자에서 Vancomycin 내성 장구균의 직장내 감시 배양 연구

김명신,이승옥,임지향,서광수,김용구,한경자,최정현,유진홍,최수미,신완식,이동건,김경미 대한감염학회 2003 감염과 화학요법 Vol.35 No.3

목적 : Vancomycin 내성장구균(VRE)은 병원감염의 중요한 원인균으로 VRE 보균상태의 환자들을 파악하여 감염을 예방할 수 있는 감시체계가 필요하다. 본 연구에서는 혈액종양병동 입원 환자를 대상으로 VRE의 직장내 감시배양을 실시하여 그 빈도 및 분포를 알아보고, 집락화된 균주와 임상검체에서 분리된 VRE와의 관계를 알아보고자 하였다. 방법 : 가톨릭대학교 성모병원 혈액종양병동에 입원해 있는 환자를 대상으로 14개월간 직장 감시배양을 시행하였다. 혈액한천배지에서 장구균이 의심되는 집락을 따서 vancomycin 6㎍/㎖이 포함된 Brain-heart infusion agar에 10^(6) CFU를 접종하여 vancomycin 내성선별검사를 하였다. 이 기간 동안 4병실에서 환경감시배양을 시행하였다. 분리된 균주는 Microscan을 이용하여 균종을 동정하였고 vancomycin에 대한 최소억제농도를 측정하였다. Multiplex PCR로 균종 확인 및 동시에 vancomycin 내성 유전형을 알아보았다. 결과 : 대상이 된 452명의 환자 중 230명(50.9%)이 직장내 VRE 보균자로 확인되었다. 균종의 분포는 E. faecium (72.4%), E. faecalis(24.1%) 순으로 vancomycin에 대한 MIC가 128㎍/㎖ 이상의 고도 내성이었으며, vanA형이 대부분이었다. 환경 감시배양에서는 2개 병실에서 VRE 4주가 분리되었다(E. faecium 3주, E. faecalis 1주). 감시기간동안 32명의 환자의 임상검체에서 33주의 VRE가 동정되었고 이 중 29명(90.6%)이 직장내 VRE 보균자였다. 결론: 가톨릭의대 성모병원 혈액종양병동의 VRE의 직장 내 집락화율이 높음을 알 수 있었다. 특히 임상검체에서 VRE가 분리된 환자의 경우 VRE 직장내 집락화율이 유의하게 높게 나타나 보균상태의 VRE가 환자의 감염에 직접적인 연관이 있을 수 있으므로 이를 예방하고 관리하는 지침이 필요하다고 생각한다. Background: Vancomycin-resistant enterococci (VRE) have emerged as important pathogens among hospitalized patients. We evaluated the prevalence of rectal VRE colonization at hematology-on-cology unit and the correlation between VRE colonization and infection. Materials & Methods: All VRE isolates were obtained from rectal swabs and clinical samples of the patients hospitalized over 14-month period at hematology-oncology unit in St. Mary's Hospital. The agar screening test was performed by using 6 ug/ml of vancomycin in brain heart infusion agar with 10^(6) CFU inoculum. During this period, the environmental surveillance culture was performed in 4 hospital rooms. Species identification of VRE was done by Microscan and MICs to vancomycin. Identification and genotyping of VRE pattern were confirmed by multiplex PCR. Results: Rectal colonization rate was 50.9% (230 among 452 patients). The most common isolate was E. faecium (72.4%) followed by E. faecalis (24.1%), and all had vanA genotype. Four VREs (three E. faecium and one E. faecalis) were isolated from 2 hospital rooms. During this period, 33 clinical VRE strains from 32 patients were isolated (infected or colonized). Twenty nine (90.6%) of these clinical isolates came from VRE carriers. Conclusion: This study showed a high rectal colonization rate of VRE in patients hospitalized at the hematology-oncology unit in St. Mary's Hospital. The colonization rate of VRE in the rectum was higher among the patients who had clinical infection by VRE. Therefore, the strategies for prevention and control of VRE infection should be deveoloped and monitored.