배양계배 근아세포의 MyoD 발현에 대한 N-cadherin의 영향

안병태,진경실 가톨릭대학교 자연과학연구소 1999 자연과학논문집 Vol.20 No.-

근아세포는 여러 단계의 근육형성 프로그램을 거쳐 다핵의 근관세포를 형성하며, 근특이 단백질의 합성 및 축적이 일어난다. 근특이 단백질의 합성은 유전자 조절 단백직인 MyoD, myogenin, Myf-5, 그리고 MRF-4 등에 의하여 조절되며, 이들 단백질이 동형접합체나 이형접합체를 형성함에 따라 DNA와 결합하여, 전사를 조절한다. MyoD는 유전자의 발현 시기와 장소를 결정하는 단백질로, 근세포가 아닌 세포를 근세포로 전환시키는 능력이 있으며, 근세포 분화에 관여한다. 근아세포가 응집을 하기 위해서는 cell adhesion molecule 등이 필요하며, 이 과정에 칼슘의존적 cell-cell adhesion molecule로서 생쥐와 닭의 뇌세포에서 발현되는 N-cadherin이 관여한다. 신경세포내의 cadherin 발현양상에 따라 신경세포가 운동신경과 감각신경으로 분화되며, anti-cadherin antibody를 처리했을 때 팔, 다리의 연골형성이 억제된다. N-cadherin은 골격근세포나 그밖의 많은 세포에서도 태아시기에 일시적으로 발현되며, 분화 초기에 근아세포에서 발현되어 근세포 간의 접합 등에 관여한다. 또한, 세포의 접합 뿐아니라 융합, 이동, 배열 등에 관여한다고 보고된 바 있다. 본 연구는 N-cadherin이 근육에서 일시적으로 발현되는 것에 초점을 맞추고, anti-N-cadherin antibody(NCAD2)를 사용하여 N-cadherin을 억제함으로써 발생하는 근육 분화의 형태적, 생화학적 변화를 분석하였다. 근아세포 성장에 NCAD2를 처리함에 따라 형태적 분화양상의 차이가 관찰되었고, 융합의 정도가 감소되어 나타났다. 또한 NCAD2를 처리했을 때, 근세포 분화 동안 발현되는 MyoD의 발현이 억제됨을 관찰할 수 있었다. 이러한 결과들은 세포 상호작용을 중재하는 N-cadherin이 근세포 분화에 요구되는 신호전달에 중요 역할을 하는 것을 시사한다. N-cadherin is a Ca^(2+)-dependent cell-cell adhesion molecule. It was identified in brain cells of mouse and chicken. Its expression is transient in skeletal muscles and many other tissues. Myoblasts grown in the presence of anti-cadherin antibodies(NCAD-2) exhibited an altered morphology compared to control cultures, coupled with decreased myoblast fusion. Treatment with NCAD-2 suppressed MyoD expression in muscle cell differentiation. These results show that cadherin-mediated cell interaction plays a critical role in the signaling events required for differentiation of muscle progenitor cells.

약물에 의해 미세사의 기능장애를 받은 배양간세포의 미세구조 변화

김진국,고정식,안의태,박경호 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.5

결정형 규산분진에 폭로된 섬유모세포의 자가증식 : Evaluation by H₂O₂and PDGF-AA 와 TGFβ

안병용,김경아,문제혁,정진숙,김은경,임영 대한산업의학회 2000 대한직업환경의학회지 Vol.12 No.2

Objectives : The aim of this study is to find out the activity of autoproliferation of rat fibroblast exposed to crystalline silica and the role of mediators secreted from rat fibroblast. Methods : The effect of α-quartz on production of growth factor (platelet-derived growth factor-AA and transforming growth factorβ) from rat fibroblasts were evaluated by ELISA and immunocytochemical analysis. Gene expression of these growth factors in rat fibrobast exposed to crystalline silica was evaluated by RT-PCR. Furthermore, fibroblast proliferation by culture supernatant of rat fibroblast was assayed by the neutral red test. Results : The amounts of H2O2 and growth factors synthesized in rat fibroblasts were significantly increased by the stimulation of crystalline silica (α-quartz) , which showed the dose-dependent manner to the concentration of α-quartz with the maximum response at the dosage of 100 ㎍/㎠. The result of RT-PCR demonstrated that α-quartz induced gene expression of PDGF-AA and TGFβ in rat fibroblast. We also found that supernatant of α-quartz-cocultured rat fibroblast induced a significant proliferation of fibroblast. Conclusion : Crystalline silica directly induce functional change In fibroblast such as increased release of reactive oxygen species and growth factors. The products of these functional change promote fibroblast proliferation via autocrine loop.

유성견의 소구치 압하시 초기 치아 및 치주 조직의 조직학적 변화와 재생에 관한 연구

안병교,차경석,이진우 대한치과교정학회 1998 대한치과교정학회지 Vol.28 No.2

치아에 압하력을 elastic chain을 이용하여 지속적으로 교정력을 평균 9개월된 유 성견의 소구치에 가한 직후 치아와 그 주위 조직에서 일어나는 조직학적인 변화와 압하력을 제거한 후 일정기간이 지난 후 변화되었던 조직의 회복양상을 고찰하고, 이를 토대로 교정치료를 위한 생물학적인 근간을 마련하기 위하여 본 연구를 시행하였으며, Hematoxylin & Eosin 및 Masson's Trichrome염색법을 이용하여 치아와 주위조직의 반응을 치경부, 치근 중앙부, 치근단부로 나누어 광학 현미경하에서 관찰하여 다음과 같은 결론을 얻었다. 1. 대조군의 HE 소견에서 치주인대는 치근단부에서 치근경부까지 비교적 균일한 두께를 나타냈고, 치주인대의 주행방향은 치근경부에 수평 또는 약간 경사져 있었으며, 치근 중앙부와 치근단부에서는 사선으로 주행하였다. 2. 대조군의 MT 소견에서 치경부에서는 조골세포 및 파골세포가 나타났으며 치경부, 치근 중앙부 그리고 치근단부에 약한 골흡수와 신생골 침착이 보였다. 3. 실험1군에서는 치근단부에서 뚜렷한 파골세포의 증가와 혈관 충혈이 보였으며, 신생골 형성과 불규칙한 반전선들이 나타나 골흡후하는 양상을 보였다. 치주인대는 치경부에서 치근단에 이르기까지 뚜렷한 증가를 보였으며 치근단부에서 더 심하였다. 4. 실험 2군에서는 대조군에 비해 조골세포가 치근단부에서 혈관 충혈과 함께 여전히 관찰되었다. 치주인대 폭경은 치근단부에서 대조근에 비해서는 증가되었고 1군에 비해서는 감소된 양상을 보였다. 치주인대 주행방향은 1군과 같았고 단지 치근단부에서 혼합된 양상으로 관찰되었다. 이상과 같은 소견으로 보아 유성견 하악 소구치 압하시 초기 조직변화에서 압박을 받는 치근단부에서 파골세포의 뚜렷한 증가, 출혈상 및 간격확장, 그리고 골흡수와 백악질 흡수가 현저히 나타났으며, 치주인대 주행방향도 다양하였다. 또한 압하력을 제가한 후에 변형되었던 치아와 치아 주위조직들이 완전한 회복 양상을 보이지는 않았다. The purpose of this study was to investigate the initial tissue change, to repair on the teeth & surrounding tissue under the intrusive orthodontic forces by use of elastic chain, through the microscopic findings. For this study, three young adult mongrel dogs were used, and were divied into three group; the control group was delivered only casting crown, and the experimental group 1 was equipped with energy chain during 1 week, and experimental 2 group was delivered using energy chain during 1 week, and 3 weeks observation. All experimental groups and control groups were sacrificed to make the samples for microscopic findings on premolar teeth. All samples were examed and compared the histologic changes through the microscopic with H-E stain. The obtained results were as follows. 1. In hematoxylin-eosin stain of the control group, the periodontal ligament was constant width from apical third to cervical third of the root, and the periodontal fiber arrangement was horizontal or oblique in cervical third, oblique in middle and apical third of the root. 2. In Masson Trichrome stain of the control group, osteoblast and osteoclast appeared in cervical third of root, and bone resorption and new bone formation was observed in middle and apical third of the root. 3. In experimental 1, osteoclasts were increased highly, and hyperemia of blood vessels and new bone formation and bone resorption by reversal line in apical third of the root were seen. PDL width was increased apprarently from crest to apex of the root and more in apical third. 4. In experimental 2, osteoclasts and hyperemia of blood vessels were more increased than control material in apical third of the root. PDL width was increased more than control group in root apex, and was seen less than experimental 1. PDL arrangement was similar to experimental 1 and was mixed only in root apex. Therefore, in premolar intrusion of the young adult dog, there were increased osteoclast, hyperemia and dilation of blood vessel, resorption of alveolar bone and cementum, and different arrangement of PDL in initial tissue change. There was not observed complete repair after remove intrusive force.

머리에 방사선 조사를 받은 흰쥐 신경뇌하수체의 미세구조 연구

안의태,김표년,양남길,고정식,박경호,김진국 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.1

十二變에 대한 考察 : 六元正紀大論을 중심으로

채영진,남호현,이석모,여성원,한성규,이재원,안민식,정헌영,금경수 한국전통의학연구소 2003 한국전통의학지 Vol.13 No.1

The six kinds of natural factors(It refers to wind, cold, summer-heat, dampness, dryness and fire.) is constantly moving to maintain the balance of whole climate of one year. Four seasons are the largest mediation of whole climate of one year. The cold and the heat have the relationship of mutual intervention. The dampness and the dryness have it also. In this process they raise various climate appearance. And that various climate appearance influence the whole lives on the earth. This paper is concerned with the classification of them.

Satellite-Pulse Technique(SPT)를 이용한 결함의 크기측정

곽경진,안희성,정용무,이영필 韓國非破壞檢査學會 1981 한국비파괴검사학회지 Vol.1 No.1

소위Satellite-Pulse Technique(SPT)을 이용하여 인공결함의 크기를 측정하였다. 측정치와 실제치를 비교하였는바 오차는 무시할 정도이었다. Sizing of artificial flaws is accomplished by ultrasonic Satellite-Pulse Technique(SPT). Negligible error is found.

사상체질과 신체형태지수와의 연관성

박경아,김희진,허경석,안수경,이성호,이종은,이원택 대한췌담도연구회 2000 대한췌담도연구회지 Vol.5 No.4

남자대학생의 흡연태도와 행동에 관한 연구

강경진,김정미,민혜영,안소진,오경숙,은경희,이현,이희선,홍주미,김수지 이화여자대학교 간호과학대학 2003 이화간호학회지 Vol.- No.37

There have been so many researches about problems of cigarettes and relation of smoking and heath since 1950's and generally known that smoking is one of the worst factor of increasing disease and mortality. No matter what such many proofs about various problems of smoking have been researched, smoking rate of current male collage students has not been decreased. Besides, most of the researches about cigarette use and its addiction have been focused on the adolescent or adult women smokers or adult male, not male collage student. Therefore, the subject of this study is about smoking attitude and smoking behavior of male collage students by general features. Smoking attitude was analyzed by modifying 'Guidelines for the content of tobacco smoking surveys of the general population'(WHO, 1982). Two hundred of male collage students participated to the distributed questionnaire for one week in May. The smoking attitude was categorized into duration of smoking and number of cigarette per day(CPD), and nicotine dependency, investigated by Fagerstrom Tolerance Questionnaire(FTQ) (Fagerstorm & Schneider, 1989). All statistics were performed by procedure of SAS according to number, percentage, mean, standard deviation, and ANOVA. The results are as follows: 1. Smoking attitude level was mean 25.73±5.15 marks, ranging from 13 to 39 marks of the total 40 marks. 2. Duration of cigarettes, number of cigarette per day, and nicotine dependency were mean 5.14±2.84 years, 12.88±6.85 pieces, and 4.78±1.69, respectively. 3. Smoking attitude showed significance according to general features such as marital state and allowance, but the significance according to marital state is not valuable because the numerical difference is so big between married one and single that they cannot be compared with each other. 4. Duration of smoking was significant according to general features such as age and allowance. 5. Smoking amount showed significance the factors of age only. 6. ll1ere was no significance in nicotine dependency according to general features. As a result, smoking attitude of male collage student in this study shows moderate level. Smoking attitude and duration is significant according to allowance, and smoking amount is significant according to age. Nicotine dependency according to general features is not significant but comparing with the preceding study about adult male, male collage students in this study are respectively more nicotine-dependent than adult male smokers. In conclusion, the right allowance usage strategies and effective heath education focused on the young are needed. In addition, helpful programs and information spreading strategies are necessary for establishing higher level of smoking attitude. At last, systemic regulations in daily living for ""anti-nico-dependent"" and perspective reforming of male university student are needed for reducing nicotine dependency.

BCG 접종후의 결핵반응검사의 변화

김진경,김기홍,김행미,안두홍 慶北大學校 醫科大學 1991 慶北醫大誌 Vol.32 No.1

신생아 시기에 BCG를 접종받은 영아를 대상으로 3±개월, 7-8개월 및 15-16개월에 Mantoux 검사를 시행하여 다음과 같은 결과를 얻었다. 3-4개월째 측정한 결절의 크기는 5.8±4.5㎜, 7-8개월째 7.9±4.9㎜,15-16개월째 6.7±4.9㎜로서 7-8개월째 의의있게 (p<0.55)증가되어 있었다. Mantoux 검사상 결절의 크기가 5㎜미만인 경우를 음성으로 간주하였을대 3-4개월째 38.5%, 7-8개월째 26.0% 및 15-16개월째 35.2%가 음성이었으며 15㎜이상의 강양성은 3-4개월째 1.9%, 7-8개월째 12%, 15-16개월째 7.5%를 차지하였다. 33례의 미숙아에 있어서 BCG 접종후 18개월 동안 5㎜ 이상의 반응을 보인 양전례는 63.6%인 21례로 이들 양전군과 비양전군 사이에는 출생시 제태연령, 체중 및 BCG 접종시기에 유의한 차이를 보이지 않았으며 만삭아의 양전율과 비교 하였을 때 의의있는 차이를 보이지 않았다. We conducted this study to evaluate the change of tuberculin reaction after neonatal BCG vaccination in Korea. Seventy-four infants were followed for 15-16 month after birth. BCG vaccination were given within 4 weeks after birht and 3 times of Mantoux test were performed at 3-4 months, 7-8 months and 15-16 month after birth. During the same period, Mantoux test were done on 238 cases of 3-4 months-old infants, 107 cases of 7-8 months-old infants and 85 cases of 15-16 months-old infants who were given BCG vaccination within 4 week after birth. We evaluated the results without distinction of these infants because there was no statistically significant difference between two groups. The size of induration was significantly increased at 7-8 months(7.9±4.9㎜) compare to the size at 3-4 months (5.8±4.5㎜) and 15-16months(6.7±4.9㎜) (P<0.05). But 38.5%, 26.8% andn 32.5% of infants were not converted ( < 5㎜) at 3-4months, 7-8 months and 15-16 months after birth respectively. Thirty-three preterm infants were followed for 18 months after birth. Twenty-one out of 33 (63.6%) were converted during this period. There was no difference of gestational age, birth weight, age on BCG vaccination between converters and nonconverters. There was no difference of conversion rate compatre to full term infants.