Transcriptional activation of hypoxia-inducible factor-1α by HDAC4 and HDAC5 involves differential recruitment of p300 and FIH-1

Seo, Hee-Won,Kim, Eun-Jin,Na, Hyelin,Lee, Mi-Ock Elsevier 2009 FEBS letters Vol.583 No.1

<P><B>Abstract</B></P><P>The interplay between hypoxia-inducible factor-1α (HIF-1α) and histone deacetylase (HDACs) have been well studied; however, the mechanism of cross-talk is unclear. Here, we investigated the roles of HDAC4 and HDAC5 in the regulation of HIF-1α function and its associated mechanisms. HDAC4 and HDAC5 enhanced transactivation by HIF-1α without stabilizing HIF-1α. HDAC4 and HDAC5 physically associated with HIF-1α through the inhibitory domain (ID) that is the binding site for factor inhibiting HIF-1 (FIH-1). In the presence of these HDACs, binding of HIF-1α to FIH-1 decreased, whereas binding to p300 increased. These results indicate that HDAC4 and HDAC5 increase the transactivation function of HIF-1α by promoting dissociation of HIF-1α from FIH-1 and association with p300.</P><P><B>Structured summary:</B></P><P>MINT-6802187:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>FIH1</I> (uniprotkb:Q9NWT6) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802058:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>HDAC4</I> (uniprotkb:P56524) by <I>pull down</I> (MI:0096)</P><P>MINT-6802021:</P><P><I>HIF1 alpha</I> (uniprotkb:Q61221) <I>physically interacts</I> (MI:0218) with <I>HDAC4</I> (uniprotkb:P56524) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802036:</P><P><I>HIF1 alpha</I> (uniprotkb:Q61221) <I>physically interacts</I> (MI:0218) with <I>HDAC5</I> (uniprotkb:Q9UQL6) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802102:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>HDAC5</I> (uniprotkb:Q9UQL6) by <I>pull down</I> (MI:0096)</P><P>MINT-6802121, MINT-6802156:</P><P><I>P300</I> (uniprotkb:Q09472) <I>physically interacts</I> (MI:0218) with <I>HIF1 alpha</I> (uniprotkb:Q16665) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P>

Function of the pentose phosphate pathway and its key enzyme, transketolase, in the regulation of the meiotic cell cycle in oocytes

Kim, Yunna,Kim, Eun-Young,Seo, You-Mi,Yoon, Tae Ki,Lee, Woo-Sik,Lee, Kyung-Ah The Korean Society for Reproductive Medicine 2012 Clinical and Experimental Reproductive Medicine Vol.39 No.2

Objective: Previously, we identified that transketolase (Tkt), an important enzyme in the pentose phosphate pathway, is highly expressed at 2 hours of spontaneous maturation in oocytes. Therefore, this study was performed to determine the function of Tkt in meiotic cell cycle regulation, especially at the point of germinal vesicle breakdown (GVBD). Methods: We evaluated the loss-of-function of Tkt by microinjecting Tkt double-stranded RNAs (dsRNAs) into germinal vesicle-stage oocytes, and the oocytes were cultured in vitro to evaluate phenotypic changes during oocyte maturation. In addition to maturation rates, meiotic spindle and chromosome rearrangements, and changes in expression of other enzymes in the pentose phosphate pathway were determined after Tkt RNA interference (RNAi). Results: Despite the complete and specific knockdown of Tkt expression, GVBD occurred and meiosis was arrested at the metaphase I (MI) stage. The arrested oocytes exhibited spindle loss, chromosomal aggregation, and declined maturation promoting factor and mitogen-activated protein kinase activities. The modified expression of two enzymes in the pentose phosphate pathway, Prps1 and Rbks, after Tkt RNAi and decreased maturation rates were amended when ribose-5-phosphate was supplemented in the culture medium, suggesting that the Tkt and pentose phosphate pathway are important for the maturation process. Conclusion: We concluded that Tkt and its associated pentose phosphate pathway play an important role in the MI-MII transition of the oocytes' meiotic cell cycle, but not in the process of GVBD.

Descriptive and Hedonic Perception of Consumers for Instant Drinking Porridges

Kim Young Seo1,You Su MI,Oh Ji Eun,Cho Mi Sook 한국식생활문화학회 2018 한국식생활문화학회학술대회논문집 Vol.2018 No.10

Increased Urinary KIM-1 and Tubular KIM-1 Expression in IgA Nephroapathy (초)

( Mi Seon Seo ),( Soon Hyo Kwon ),( Moo Young Park ),( Soo Jung Choi ),( Jin Seok Jeon ),( Hyun Jin Noh ),( Jin Gook Kim ),( Dong Cheol Han ),( Seung Duk Hwang ) 대한내과학회 2010 대한내과학회 추계학술대회 Vol.2010 No.-

울릉도 소의 전염성 병원체 감염률 조사

서민구 ( Min Goo Seo ),도재철 ( Jae Cheul Do ),우인옥 ( In Ohk Ouh ),조민희 ( Min Hee Cho ),김중규 ( Joong Kew Kim ),김영환 ( Young Hoan Kim ),박노찬 ( No Chan Park ),곽동미 ( Dong Mi Kwak ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.4

Prevalence of infectious agents, including Brucella abortus (BA), Mycobacterium bovis (MB), bovine leukemia virus (BLV), M. avium subsp. paratuberculosis (MP), Neospora caninum (NC) and Toxoplasma gondii (TG), was investigated in all the cattle raised in Ulleung island during 2007∼ 2010. For BA, the prevalences in head and farm were 8.1% (44/545) and 5.5% (4/73) in 2007, all negative in 2008∼2009, and 0.5% (4/774) and 1.7% (1/58) in 2010, respectively. For MB, no sample was positive by PPD or ELISA in 2007∼2010. For BLV and MP, no sample was positive by ELISA in 2007∼2009. For NC, seroprevalences in head and farm were 0.2% (1/545) and 1.4% (1/73), respectively, in 2007 and all negative in 2008∼2009. For TG, seroprevalences in head and farm were 17.6% (97/552) and 54.8% (34/62) by ELISA in 2009. By regions, the seroprevalences of TG in Ulleung-eup, Seo-myeon and Buk-myeon were 26.0%, 9.8% and 16.7%, respectively, which had significant differences (P<0.0001). Tiger cattle were more resistant to TG infection than Hanwoo. The seroprevalence of TG in summer was higher than in autumn. The seroprevalence of TG in cows was higher than in oxen. The seroprevalence of TG in cattle was increased with age. In conclusion, this study indicates that the prevalences of six infectious diseases, except for TG which are widely spread, are relatively low in cattle reared in Ulleung island.

암 병동 간호사의 통증관리에 대한 지식과 태도

공성화,방찌야,서민숙,현신숙,김희정,이미애1,이미애2,유현희,허재경,김은애,박경숙 성인간호학회 2004 성인간호학회지 Vol.16 No.1

Purpose: The purpose of this study is to evaluate the knowledge and attitudes of oncology ward nurses toward cancer pain management and to find ways to improve the educational program for nurses. Method: A total of 209 nurses working at the oncology ward of three hospitals in Seoul and a Gyenggi Province. The survey instrument used was the 32-item scale for evaluating nurses knowledge and attitudes originally developed by McCaffery and Ferrell'(1990). that was by Kim'(1997). Result: In terms of the nurses knowledge of pain management. the result showed that the nurses scored an average of 67.8 out of 100 for phamacokinetics of opioids. 84.8 for classification of analgesics, 60.1 for pain assessment, and 70.7 for drug administration. 18.2% of the nurses hesitate to inject the narcotic agent because of concerns regarding the drug's potential side effects. there was significant difference in the knowledge of pain management according to the general characteristics of pain in terms of the nurses age(p=.001), position (p=.016). years of experiences(p=.002), experience of cancer pain education(p= .001). Conclusion: The also showed that nurses working at cancer ward lack knowledge. It is important to provide intensive education to nurses about cancer pain management.

읽기장애 아동의 음운처리 능력 특성 연구

김미경,서경희 한국특수교육문제연구소 2003 특수교육저널 : 이론과 실천 Vol.4 No.4

최근 읽기장애와 언어와의 관계의 중요성이 부각되면서 음운처리 결함이 읽기장애의 원인으로 널리 받아들여지고 있다. 이에 본 연구는 2학년, 4학년 일반아동 29명과 읽기장애아동 23명을 대상으로 음운인식능력 검사와 단어회상하기 검사, 문장회상하기 검사, 글자, 숫자, 사물, 색깔명명하기 검사를 통한 읽기장애아동들의 음운처리능력 특성을 연구하였다. 연구 결과 읽기장애아동들은 일반아동들에 비해 음운인식, 작업기억, 명명속도에서 유의미한 차이를 보였다. 이러한 결과를 바탕으로 일반학급과 특수학급에서 읽기장애 아동들을 지도하는 프로그램개발에 기여할 것을 제안한다. The purpose of this study was to investigate difference between normal children and children with reading disabilities, ranged from grade 2 to grade 4 in phonological processing ability. The sample was classified into two groups: (1) a group of 23 children with reading disabilities; (2) a group of 29 normal children. Students in the sample of this study do not have severe emotional problems, uncorrected vision problems, hearing loss, acquired neurological disorders. The results of this study were as follows: 1.Children with reading disabilities performed significantly poorly on test of phonological awareness than children without reading disabilities. 2.Children with reading disabilities performed significantly poorly on test of working memory than children without reading disabilities. 3.Children with reading disabilities performed significantly poorly on test of naming speed than children without reading disabilities. There is also interaction effect between the presence or absence of reading disability and grade on naming speed.

가베 활용 프로그램이 유아의 다중지능에 미치는 효과

김미정,서영숙 숙명여자대학교 아동연구센터 2008 兒童硏究 Vol.21 No.1

이 연구의 목적은 가베를 활용하여 유아의 다중지능 향상에 필요한 프로그램을 개발하고 그 프로그램을 유아교육현장에 적용하여 유아들의 다중지능 증진에 미치는 효과를 알아보고자 하는데 있다. 연구 대상은 서울특별시 노원구에 소재한 A유치원에 다니는 만 4.5세 유아반 5개 반 유아를 실험집단 (48명)과 통제집단(48명)으로 무선 표집 하였다. 다중지능 검사 도구는 서울대학교 교육연구소(1997)에서 만든 유아용 다중지능 검사지 (7개영역)와 문용린 외 (2001)가 개발한 다중지능 검사지(1개영역-자연지능)를 합한 검사지를 사용하였다. 수집된 자료는 SPSS-WIN 10.0을 사용하여 t검증을 실시하였다. 이 연구의 결과는 다음과 같다. 첫째, 가베 활용 프로그램은 다중지능 여덟 개 영역으로 나누어서 개발 및 구성하였다. 다중지능 영역별 프로그램은 음악 지능 21개, 논리-수학 지능 14개, 언어 지능 13개, 신체-운동 지능 6개, 공간 지능 9개, 대인관계 지능 10개, 개인 내 지능 8개, 자연 탐구 지능 10개이다. 둘째, 가베 활용 프로그램을 처치한 실험집단의 유아들이 자유 선택놀이를 한 통제집단의 유아들 보다 다중지능검사도구의 접수가 다중지능 여덟 개 영역에서 모두 유의하게 높게 나타났다 프로그램 진행 과정에서도 유아들이 기본 활동 외에도 스스로 활동을 응용하고 확장하는 모습을 보여 주었으며 이런 활동들을 통하여 유아들의 잠재적인 능력을 끌어내어 다중지능을 향상 시킬 수 있었다. 이러한 연구 결과를 비추어 볼 때, 가베 활용 프로그램은 유아의 다중지능 향상에 효과적이며, 유아교육현장에서 활용할 수 있는 교육적인 프로그램으로서 유아의 다중지능 증진에 긍정적인 기여를 할 수 있을 것으로 본다. The purposes of this study are to develop the Gabe-based educational programs which are useful for enhancement of Multiple Intelligences ofchildren and to apply them to the kindgarten classrooms to study the effects of them. We selected 96 4~5 year olds from 5 classes of A kindergarten which is located in Nowon District, Seoul city. And we also separated the young children into a test group and a control group each composed of random 48 children. We used the Multiple Intelligence Test for Infants made by the Education Research Institute of Seoul National University(1997) and other Multiple Intelligence Tests developed by Moon Yong Rin(2001) based on the Shearer, C. B.(1991) development screening which is a test for an infant's multiple intelligences. Also the statistics program SPSS win 10.0 was used and T-verification was employed to analyze the data. The conclusion for the test results are as follows: First, the program is developed upon and composed of 8 intelligence dimensions(linguistic, logical-mathematical, musical, spatial, bodily-kinesthetic, inter-personal, intrapersonal, naturalist intelligences) Second, the children in the test group exposed to the Gabe-based programs geared to develop multiple intelligences showed a statistically significant higher grades in all 8 dimensions compared to the children in the control group which were exposed only to free plays. Based on the results of this study, we can arrive to the conclusion that the Gabe-based educational programs are useful for enhancement ofmultiple intelligences of young children when applied to kindergarten classrooms.

CATV 홈쇼핑에서의 충동구매 자극 요인과 구매 경험과의 관련성

서미정,김혜선 대한가정학회 2004 Human Ecology Research(HER) Vol.42 No.3

Cable TV Home Shopping is one of the popular distribution channels in modem society because of purchasing convenience and saving in shopping time. However, in spite of these advantages, there are same problems because of marketing strategies of many Cable TV shopping companies which stimulate impulse purchases. This study is aimed to f i i the relationship between buying experience and reaction to impulsive buying stimulus. This study can give a valuable insight in consumer education regarding impulsive purchase behavior in Cable TV home shopping. Study results can also give useful information on the direction of consumer campaign and consumer protection policies. The data was collected by using a questionnaire and the final 496 cases were analyzed by SPSS Win 100 program. The major finding were that 1. Younger, more educated and unmarried consumers tend to response more to impulsive purchasing stimulus. 2. consumers with higher response to impulsive purchasing stimulus had more buying experience, higher shopping frequencies and spent more money in Cable TV home shopping than consumers with lower response. These study results showed that there are some relationship between impulse purchasing stimulus and buying experience in Cable TV home shopping. Therefore, Cable TV Home Shopping companies should provide correct information to the consumers, so they can make resonable decisions. And the consumers should also try to restrain themselves from purchasing impulsively. A law that will regulate all false or exaggerated ads should be provided. To reach righteous and beneficial home shopping culture for both consumer and companies, all these means should be cooperated.

Polyaaccharide 類의 固形分斯體에 의한 藥物 傳達體系의 開發

서성훈,김길수,김동현,이승진,장미경 경희대학교 약학대학 1994 慶熙藥大論文集 Vol.22 No.