배양세포주(MDBK cell)내에 caffeine, 철분 및 vitamin E 혼합처리시 배양시간 경과에 따른 지질과 단백질 구성성분의 변화

도재철,허린수,Do, Jae-cheul,Huh, Rhin-sou 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.4

This study was conducted to identify the effects of caffeine or combinations of caffeine and iron or vitamin E on the lipid and protein components in the MDBK(Mardin-Darby Bovine Kidney) cells. For the In vitro test, MDBK cells in ${\alpha}$-MEM(Minimum Essential Medium) were divided into 4 treatment groups according to drug types and dosages as follows; the control(group A), group B was treated with 0.3mM caffeine, group C was treated with 0.3mM caffeine and 0.3mM ferric chloride, group D was treated with 0.3mM caffeine and 0.3mM vitamin E. Those groups were further divided into 5 subgroups according to the time lapsed(control, 4hrs, 8hrs, 24hrs and 48hrs lapsed group). The concentrations of the carbonyl group and malondialdehyde(MDA) and the patterns of the SDS-PAGE(Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) and fatty acid compositions were analyzed to determine the oxidative damages and metabolic changes on the lipid and protein components in the MDBK cells. The results obtained from this study were summarized as follows; 1. The concentrations of carbonyl group and malondialdehyde in MDBK cells of group C were significantly higher(p<0.01) in comparison to the control, and increased according to the time lapsed. But the results of groups B and D were little different in comparison to the group C. 2. As the analytical results of fatty acid compositions in MDBK cells, the proportions of palmitoleic acid and linoleic acid in groups B, C and D were lower in comparison to the control, while the proportion of arachidonic acid in groups B, C and D were significantly higher(p<0.01) in comparison to the control. 3. In order to determine the oxidative damages to the protein in MDBK cells, the patterns of the SDS-PAGE were examined and the patterns of SDS-PAGE in groups C and D were significantly different between 43kd and 200kd of molecular weight.

Caffeine, 철분 및 vitamin E 혼합투여시 rat의 혈액과 간조직내에서 혈액화학성분과 지질 및 단백질 구성성분의 변화

도재철,허린수,Do, Jae-cheul,Huh, Rhin-sou 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.3

This study was conducted to identify the effects of caffeine or combinations of caffeine and iron or vitamin E on the lipid and protein components or blood chemistry levels of the serum as well as the total homogenate, mitochondrial and microsomal fraction of the rat(Sprague-Dawley, female) liver. Chronic test were conducted to determine those effects. The chronic test was conducted by dividing rats into 5 groups according to the type of drugs and dosages administrated as follows; the control(group A), and group B was given 25mg/kg caffeine orally once daily for 30 days, group C was given 50mg/kg caffeine orally once daily for 30 days, group D was given 25mg/kg caffeine and orally ferric chloride once daily for 30 days and group E was given 25mg/kg caffeine and 25mg/kg vitamin E once daily for 30 days. The concentrations of glucose, urea nitrogen, uric acid, creatinine, total protein, albumin, A/G ratio, triglyceride, total cholesterol, HDL-cholesterol, free fatty acid, phospholipid as well as the activities of alanine aminotransferase(ALT), aspartate aminotransferase(AST) and alkaline phosphatase(ALP) were measured in the serum of each experimental groups. The concentrations of the carbonyl group and malondiaidehyde(MDA) and the patterns of the SDS-PAGE(Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis) and fatty acid compositions in free fatty acids and phospholipids were analyzed to determine the oxidative damages and metabolic changes on the lipid and protein components in the serum, and total homogenate, mitochondrial and microsomal fractions of the rat liver. The results obtained from this study were summarized as follows; 1. Body weights of groups B, C, D and E were significantly decreased(p < 0.01) in comparison with that of the control in the chronic test. 2. The concentrations of serum glucose in groups B(124.5mg/dl), C(130.1mg/dl), D(122.1mg/dl), E(119.3mg/dl) were significantly higher(p < 0.01) in comparison to that of the control(101.5mg/dl). But, there were no significant differences in the concentrations of urea nitrogen, uric acid, creatinine, total protein, albumin and A/G ratio in comparison to that of the control. 3. The concentrations of total cholesterol and HDL-cholesterol in serum of groups B(69.6, 53.4mg/dl), C(73.0, 56.3mg/dl), D(68.9, 51.1mg/dl) and E(68.2, 51.3mg/dl) were significantly higher(p < 0.01) in comparison to that of the control(52.6, 38.8mg/dl). On the other hand, the concentrations of triglyceride in serum of groups B(45.0mg/dl), C(40.4mg/dl), D(33.8mg/dl) and E(47.2mg/dl) were significantly lower(p < 0.01) in comparison to that of the control(66.2mg/dl). There were no significant differences in the activities of ALT, AST and ALP in comparison to that of the control. 4. The concentrations of free fatty acid and phospholipid in serum of groups B(45.7, 154.4mg/dl), C(50.0, 167.2mg/dl), D(52.5, 148.4mg/dl) and E(41.1, 159.2mg/dl) were higher(p < 0.01) in comparison to that of the control(35.2, 125.3mg/dl). And the concentrations of the carbonyl group and malondialdehyde in serum of group D(1.82, 0.52nM/mg protein) were significantly higher(p < 0.01) in comparison to the control(1.53nM/mg protein). 5. The concentrations of carbonyl group in total homogenate, mitochondrial and microsomal fraction of group D(1.45, 0.94, 1.67nM/mg protein) were significantly higher (p < 0.01) in comparison to the control(1.16, 0.66, 1.27nM/mg protein). And the concentrations of malondialdehyde in the total homogenate, mitochondrial and microsomal fraction of group D(6.70, 6.10, 1.36nM/mg protein) were significantly higher(p < 0.01) in comparison to the control(5.17, 3.64, 0.68nM/mg protein). 6. As the analytical results of the fatty acid compositions of free fatty acid in serum, the proportions of stearic acid and arachidonic acid of groups B(16.52, 12.62%), C(17.52, 15.18%), D(19.73, 13.47%) and E(17.62, 13.28%) were significantly higher(p < 0.01) in comparison to the control(14.75, 7.88%), but the pr

Rat체내 Sulfamethazine 경구투여 후 시간경과에 따른 간장, 신장 및 근육내 잔류함유량 측정

도재철,Do, Jae-cheul 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.3

Sulfamethazine sodium was orally administrated to Sprague Dawley female rats(body weight: 200~250g) with the sonde caude at the dose of 20mg of sulfamethazine sodium per 100g of body weight for 3 days to investigate the depletion rate of the drug from liver, kidney and muscle of rat. The results obtained were summerized as follows; 1. The mean concentrations of sulfamethazine in liver according to the time lapsed after oral administration of the sulfamethazine sodium were decreased from 1.27ppm at day 1 to 0.28ppm at day 4. 2. Sulfamethazine concentrations in kidney according to the time lapsed after oral administration of the sulfamethazine sodium were decreased from 0.77ppm at day 1 to 0. 12ppm at day 4. 3. The mean concentration of sulfamethazine in skeletal muscle according to the time lapsed after oral administration of the sulfamethazine sodium was at or below 0.09ppm within 4 days after withdrawl of medicated solution.

Rat에 caffeine 경구투여후 시간경과별로 혈액과 간조직에서 혈액화학성분, 지질 및 단백질 구성성분의 변화

도재철,허린수,Do, Jae-cheul,Huh, Rhin-sou 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.4

This study was conducted to identify the effects of caffeine on the lipid and protein components or blood chemistry levels of the serum as well as the total homogenate, mitochondrial and microsomal fraction of the rat(Sprague-Dawley, female) liver. Acute test were conducted to determine those effects. The acute test was conducted by dividing rats into 7 groups according to the time lapsed after a single oral administration of 100mg/kg caffeine(that is control, 2hrs, 4hrs, 8hrs, 24hrs, 48hrs and 72hrs lapsed group). The concentrations of glucose, urea nitrogen, uric acid, creatinine, total protein, albumin, A/G ratio, triglyceride, total cholesterol, HDL-cholesterol, free fatty acid, phospholipid as well as the activities of alanine aminotransferase(ALT), aspartate aminotransferase(AST) and alkaline phosphatase(ALP) were measured in the serum of each experimental groups. The concentrations of the carbonyl group, malondialdehyde(MDA) and the patterns of the SDS-PAGE(Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) were analyzed to determine the oxidative damages and metabolic changes on the lipid and protein components in the serum, and total homogenate, mitochondrial and microsomal fractions of the rat liver. The results obtained from this study were summarized as follows; 1. The concentrations of serum glucose were significantly higher(p<0.01) between 4(143.0mg/dl) and 8hrs(138.0mg/dl) in comparison to that of the control(101.1mg/dl) after a single oral administration of caffeine(100mg/kg). While on the other, there were no significant differences in the concentrations of urea nitrogen, uric acid, creatinine, total protein, albumin and albumin/globulin(A/G) ratio in comparison to those of the control. 2. The concentrations of total cholesterol and HDL-cholesterol in serum were significantly higher(p<0.01) between 4(77.4mg/dl, total cholesterol) and 8hrs(64.7mg/dl, HDL-cholesterol) in comparison to those of the control(62.8, 46.7mg/dl) after a single oral administration of caffeine(100mg/kg). On the other hand, the concentrations of triglyceride in serum were significantly lower(p<0.01) after 8hrs(38.8mg/dl) in comparison to that of the control(66.5mg/dl). 3. The activities of AST in serum was significantly higher(p<0.05) from 2hrs(149U/L) to 8hrs(178U/L) in comparison to the control(112U/L) after a single oral administration of caffeine(100mg/kg). The activities of ALT in serum were significantly higher(p<0.01) at 4(45.5U/L), 24(49.3U/L), 48(46.8U/L) and 72 hrs(42.3U/L) in comparison to that of the control(39.7U/L) after a single oral administration of caffeine(100mg/kg). On the other hand, there were no significant differences in the activities of ALP in comparison to that of the control. 4. The concentrations of free fatty acid in serum were significantly higher(p<0.01) at 8hrs(65.0mg/dl) in comparison to that of the control(37.6mg/dl) after a single oral administration of caffeine(100mg/kg). However, there were no significant differences in the concentrations of carbonyl group and malondialdehyde within serum, and liver homogenate, mitochondrial and microsomal fractions in comparison to that of the control. 5. The patterns of SDS-PAGE in serum, mitochondrial and microsomal fraction of the liver showed no significant differences.

Parathion을 경구투여(經口投與)한 Mouse의 체내(體內) Cholinesterase 활성도(活性度) 및 Glucose함량(含量) 조사(調査)

도재철 ( Jae Cheul Do ),이창우 ( Chang Woo Lee ),차우양 ( Woo Yang Cha ),손재권 ( Jae Kweon Son ),정종식 ( Jong Sik Chung ) 한국가축위생학회 1992 韓國家畜衛生學會誌 Vol.15 No.1

The insecticide p-nitropheny diethyl thiophospate is alse known by the symbol E.605 and a legion of trade names including "parathion". The insecticide is widely used in agriculture, but it is highly toxic and now clear that paration behaves like a cholinergic drug by inhibiting the enzyme cholinesterase. In order to know acute toxicity and the changes of glucose concentrations and activity according to time lapsed in female mice given orally single with the half dose to LD50 of parathion, glucose contents and cholinesterase activities in serum as well as cholinesterase activities in whole brain and spinal cord were investigated, otherwise median lethal dose (LD50) of parathion given orally against female mice was determined. The results obtained were summerized as follows; 1. LD50 value of parathion given orally to female mice was 7.lmg/kg(95% confidence limits, 3.8-13.1mg/kg). 2. The inhibition rate of cholinesterase activities in serum of parathion-administrated mice according to time lapsed were peakly decreased to 61% after 30 minutes in comparison to control group, but activities were completely recovered after 48 hours. 3. The inhibition rate of cholinesterase activities in whole brain of parathion- administrated mice according to time lapsed were peakly decreased to 49% after 2 hours in completely recovered after 24 hours. 4. The inhibition rate of cholinesterase activities in spinal cord of parathion- administrated mice according to time lapsed were peakly decreased to 57% after 2 hours in comparison to control group, but activities were completely recovered after 48 hours. 5. The changes of glucose contents in serum of parathion-administrated mice according to time lapsed and in directly after death due to parathion poisoning were no significantly difference.

한우 및 돼지의 혈액화학치에 관한 연구

도재철 ( Jae Cheul Do ),이창우 ( Chang Woo Lee ),손재권 ( Jae Kweon Son ),정종식 ( Jong Sik Chung ) 한국가축위생학회 1990 韓國家畜衛生學會誌 Vol.13 No.1

In order to know the range in blood chemical values and enzyme activities of korean native cattle and pigs being raised in kyongbuk province, the mean values of serum protein compositions, enzyme activities(GOT, GP, ALP), cholesterol, glucose, bilirubin, creatinine, urea nitrogen and uric acid were determined and the results obtained were as follows: 1. Mean contents of total protein, albumin, globulin and A/G ratio for the korean native male cattle were 7.28±0.86 g / dl, 3.39±0.65 g / dl, 3.83±0.92 g / dl & 0.95±0.34: for the male pigs were 7.39± 0.84 g / dl, 3.63±0.43 g / dl, 3.77±0.88 g / dl, 0.97±0.26: for the female pigs were 7.60±0.68 g / dl, 3.54±0.58 g / dl, 3.90+0.74 g / dl, 1.02±0.34. 2. Mean activities of GOT, GPT and ALP for the korean native male cattle were 48.3±11.6 U, 29.6±4.6 U, 13.7±2.8; for the male pigs were 58.6±10.6 U. 38.6±5.3 U, 24.9±9.2 U: for the female pigs were 43.9±10.8 U, 40.5±4.6 U, 13.9±3.4 U. 3. Mean contents of cholesterol, glucose and bilirubin for the korean native male cattle were 108.7±31.8 mg/dl, 88.2+26.4 mg/dl, 0.69±0.52 mg/dl: for the male pigs were 105.9±18.3 mg/dl, 102.9±22.3 mg/dl, 0.70+0.18 mg/dl: for the female pigs were 113.1±20.9 mg/dl, 103.2±23.9 mg/dl, 0.49±0.21 mg/dl. 4. Mean contents of creatinine, urea nitrogen and uric acid for the korean native male cattle were 2.86±0.69 mg / dl, 18.1 ±5.0 mg / dl, 1.38±0.66 mg / dl; for the male pigs were 2.20± 0.51 mg / dl, 18.2±4.3 mg/dl, 0.29+0.10 mg/dl; for the female pigs were 2.36+0.70 mg/dl, 20.5±5.4 mg/dl, 0.69±0.27 mg / dl.

한우 유채중독 증례

도재철 ( Jae Cheul Do ),김은영 ( Eun Young Kim ),김인경 ( In Kyoung Kim ),조민희 ( Min Hee Cho ),김중규 ( Joong Kew Kim ),박노찬 ( No Chan Park ) 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.1

This is a case report about rape poisoning in a Hanwoo. Non-protein nitrogen (NPN) compound in rape may cause poisoning in domestic animals. Rape poisoned cattle may show big and rapid breath with opening its mouth, edema and erosion of skin with itching, drooling, dyspnea and indigestion. Besides there can be shown neurological (blindness) and urological (red water) symptoms in some cases. There is no effective therapy for rape poisoning, therefore stopping the rape supplying is basically important. Clinical symptoms and history taking are very important factors in diagnosis of rape poisoning, due to the absence of diagnostic standard of judgment for rape poisoning. A Hanwoo 38 months old cow, in a farm located in Gyeoungbuk province showed typical rape poisoning symptoms such as breath with opening its mouth, drooling, dyspnea and hyperphotosensitivity, and dramatical recovery were occurred after stopping supplying of rape. The cow were diagnosed as the rape poisoning based on observation of clinical symptoms and history taking.

국군 장병에게 급식되는 각종 식품류에 함유된 비소함량에 관한 연구

도재철 ( Jae Cheul Do ),서정유 ( Jeong You Seo ),황건택 ( Kun Taeck Hwang ),허린수 ( Rhin Sou Huh ) 국군의무사령부 1986 대한군진의학학술지 Vol.17 No.1

Vitamin E 경구투여가 한우 거세비육우의 혈액 및 근육내 지질과산화 작용에 미치는 영향

도재철 ( Jae Cheul Do ),조민희 ( Min Hee Cho ),이영미 ( Young Mi Lee ),장지택 ( Jee Taek Jang ),이양수 ( Yang Soo Lee ),손재권 ( Jae Kwon Son ),송희종 ( Hee Jong Song ),정종식 ( Jong Sik Jyeong ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.22 No.3

This study was evaluated to know the effects of vitamin E(VE) on the lipid peroxidation in blood and sirloin of castrated korean indigenous beef cattle. Experimental groups were divided into VE 500 IU(A), 1,500 IU additative feeding group(B) and non-VE-treated control group(C). After oral administration to the cattle for 120 and 150 days, body weight gains, VE contents in plasma and sirloin, and thiobarbituric acid(TBA) value were examined according to the exhibition period(1-7 days) in refrigerated showcase between aging and non- aging group. The results obtained from this study were summarized as follows; 1. Body weight gain per day of control compared with VE additative feeding A and B groups were showed no significantly differences. 2. The concentrations of VE in plasma after oral administration with VE for 120 days were significantly increased(p<0.05) in A and B groups. There were higher(p<0.05) 4.22μg/mℓ in A and 6.22μg/mℓ in B group than the control(3.0μg/mℓ). And the concentrations of VE in plasma for 150 days were significantly increased(p<0.05) in VE additative feeding groups. There were higher 4.89μg/mℓ in A and 7.05μg/mℓ in B group than the control(3.15μg/mℓ). 3. The concentrations of VE in sirloin for 120 days were significantly increased(p<0.05) in A and B groups. There were higher 1.84μg/g in A group and 2.40μg/g in B group than the control(0.78μg/g). And the concentrations of VE in sirloin for 150 days were significantly increased(p<0.05) in A and B groups. There were higher l.94μg/g in A group and 2.63μg/g in B group than the control(1.00μg/g). 4. TBA values, the indicator of lipid peroxidation, in non-aging sirloin according to the exhibition period(1-7 days) in refrigerated showcase after oral administration with VE additative feed for 120 days were lower 0.73 in A and B groups than 0.82 in control at the third day after exhibition. In the same group, TBA values were significantly(p<0.05) lower 0.77 and 0.75 in A and B groups than 1.22 in control at the seventh day after exhibition. Equally, in the aging group, there were significantly(p<0.05) showed lower TBA values 1.05 and 0.99 in A and B groups than 1.87 in control at the seventh day after exhibition. 5. After oral administration with VE additative feed to the cattle for 150 days, TBA values in non-aging sirloin according to the exhibition period(1-7 days) in refrigerated showcase were significantly(p<0.05) decreased to 0.84 and 0.88 in A and B groups than 1.26 in control at the seventh day after exhibition. In the aging group, there were significantly(p<0.05) showed lower TBA values 0.95 and 0.99 in A and B groups than 1.79 in control at the seventh day after exhibition.

Caffeine 투여시 Rat의 혈액내 혈액화학성분의 변화

도재철 ( Jae Cheul Do ),박노찬 ( No Chan Park ),장성준 ( Sung Jun Jang ),조광현 ( Kwang Hyun Cho ),박인화 ( In Hwa Park ),손재권 ( Jae Kwon Son ),김수웅 ( Soo Woong Kim ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.3

This study was conducted to identify the effects of caffeine on the change of blood chemistry components in the serum of the rat(Sprague-Dawley, female). The experimental groups were divided into 7 groups according to the time lapsed after a single oral administration of 100mg/kg caffeine(that is control, 2, 4, 8, 24, 48 and 72 hrs lapsed group) to the rats. The concentrations of glucose, urea nitrogen, uric acid, creatinine, total protein, albumin, A/G ratio, triglyceride, total cholesterol, HDL-cholesterol, free fatty acid and phospholipid as well as the activities of alanine aminotransferase(ALT), aspartate aminotransferase(AST) and alkaline phosphatase(ALP) were measured in the serum of each experimental groups. The results obtained from this study were summarized as follows; 1. The concentrations of serum glucose were significantly higher(p<0.01) between 4 (143.0 mg/dl) and 8 hrs(138.0mg/dl) in comparison to the control(101.lmg/dl) after a single oral administration of caffeine(l00mg/kg). Whereas there were no significant differences in the concentrations of urea nitrogen, uric acid, creatinine, total protein, albumin and albumin/globulin(A/G) ratio in comparison to the control. 2. The concentrations of total cholesterol and HDL-cholesterol in serum were significantly higher (p<0.01) between 4(77.4mg/dl, total cholesterol) and 8 hrs(64.7mg/dl, HDL-cholesterol) in comparison to the control(62.8, 46.7mg/dl) after a single oral administration of caffeine (100mg/kg). On the other hand, the concentrations of triglyceride in serum were significantly lower(p<0.01) after 8 hrs(38.8mg/dl) in comparison to the control(66.5mg/dl). 3. The activities of AST in serum was significantly higher(p<0.05) from 2 hrs(149 U/L) to 8 hrs(178 U/L) in comparison to the control(112 U/L) after a single oral administration of caffeine (100mg/kg). The activities of ALT in serum were significantly higher(p<0.01) at 4(45.5 U/L), 24(49.3 U/L), 48(46.8 U/L) and 72 hrs(42.3 U/L) in comparison to the control (39.7 U/L) after a single oral administration of caffeine(l00mg/kg). On the other hand, there were no significant differences in the activities of ALP in comparison to the control.