Increased Urinary KIM-1 and Tubular KIM-1 Expression in IgA Nephroapathy (초)

( Mi Seon Seo ),( Soon Hyo Kwon ),( Moo Young Park ),( Soo Jung Choi ),( Jin Seok Jeon ),( Hyun Jin Noh ),( Jin Gook Kim ),( Dong Cheol Han ),( Seung Duk Hwang ) 대한내과학회 2010 대한내과학회 추계학술대회 Vol.2010 No.-

Laccaria laccata B.의 Phytohemoagglutinin에 關한 硏究

金亨會,文國鎭 고려대학교 의과대학 1973 고려대 의대 잡지 Vol.10 No.3

The author screened twenty three kinds of mushroom native to Korea on its hemoagglutination activity to human red blood cell (RBC) and found out a new Phytohelnoagglutinin (PHA) in the saline extract of Laccaria laccata B. (LLB). Its hemoagglutination titer, absorption test with human saliva and sugars, heat stability test, salt fractionation by anmonium sulfate, sperm agglutination activity and elution test were studied. The results could be summarized as follows : 1) Anti A-lectin was found in LLB. 2) Anti A-lectin in LLB was absorbed by same group's RBC and saliva of secretor. 3) The agglutination titer of anti A-lectin in LLB were 1:8 with type A RBC and 1:4 with type AB RBC, and its optimal temperature of agglutination was 37℃. 4) The anti A-lectin was found in precipitates of LLB treated by 40% saturated amiuonium sulfate solution. 5) The agglutinating activity of anti A-fraction in LLB was inhibited by various sugars except dextrose. 6) The human sperms were not agglutinated by PHA in LLB. 7) It was possible to detect blood type A RBC in the blood stains and various humoral stains by elution test with anti A-lectin in LLB.

배양된 간세포 담세관 및 세포질소기관의 미세구조 연구 : Phalloidin과 Cytochalasin D의 영향 Effects of Phalloidin and Cytochalasin D

김진국,양남길,안의태,고정식,박경호,김주원 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

간세포 담세관의 주위세포질에는 미세사가 풍부하게 분포하는데, 이 실험에서는 배양 간세포의 미세사형성을 촉진 또는 억제시킨 후에 담세관 및 세포질소기관의 미세구조를 관찰하여 미세사의 형태학적 및 기능적 의의를 연구하였다. Sprague Dawley계 숫흰쥐의 간조직에 IV형 collagenaseㄹ를 처리하여 간세포를 분리한 후 배양하며 phalloidin(미세사 중합 촉진제)이나 cytochalasin D(미세사 중합 억제제)를 배지에 투여하고 전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1. 분리된 간세포 세포질에는 골지복합체와 소포 및 용해소체들이 세포의 일부분에 분포되어 생체의 간세포와 같이 세포질소기관 분포의 극성을 나타냈다. 2. Phalloidin와 cytochalasin D 투여군이 담세관은 확장되고 미세융모의 소실이 뚜렷하였다. Phalloidin투여군에서는 미세사가 증식되고 외형질층이 두터워져 담세관은 경직된 모습이었고, cytochalasin D투여군에서는 미세사가 감소되고 담세관의 확장은 더욱 심했다. 3. Phalloidin이나 cytochalasin D 투여군 모두에서 간세포 표면에는 세포질이 돌출되었다. Phalloidin투여군에서는 돌출부의 연결부분이 잘록하였고 외형질에 미세사가 증식되어 돌출부와 세포질을 분리시키고 있었다. 반면에 cytochalasin D투여군에서는 외형질층에서 미세사가 뚜렷한 감소되었고 각종 세포질소기관들이 포함된 채로 세포질 일부를 밀고나와 반구형의 돌출부를 이루었고 연결부는 넓었다. 4. 세포질에는 많은 소포와 공포들이 융합되어 세포의 주변부분이나 담세관주위에 세포내공간이 형성되었는데, 이 공간은 세포바깥이나 담세관과 교통되었다. 이상의 결과를 보면, 미세사가 증식 혹은 감소되면 배양간세포의 모습이 변화되며, 담즙의 이동에도 장애가 생겨 담즙정체를 일으킨다. 따라서 미세사는 간세포의 형태유지 및 담즙분비에 중요하게 관여하는 것으로 생각된다. The cytoplasmic microfilaments of hepatocytes are abundant beneath the plasma membrane, especially in the pericanalicular ectoplasm. In this study, the ultrastructural changes of bile canaliculus and cytoplasmic organelles induced by alteration of the microfilaments on the cultured rat hepatocytes were examined. Sprague-Dawley rats(male, about 200gm) were used. The isolated hepatocytes obtained by perfusion of 0.05% collagenase type IV through the portal vein, were cultred in the L-15 medium containing phalloidin(stabilizer of microfilaments) or cytochalasin D(destabilizer of microfilaments) for 10 min, 30 min, 1 hour, 2 hours, 4 hours, 10 hours and 20 hours, respecitvely. The hepatocytes on the cultured dish were fixed in 2.5% glutaradehyde -1.5% paraformaldehyde and 1% osmium tetroside. After alcohol dehydration, the cells were embedded in the araldite mixture. Ultrathin sections were contrasted with uranyl acetate and lead citrate, and observed with JEM100 CX-II electron microscope. The results were as follow: 1. Isolated hepatocytes maintained the typical architectionic relationships of secretory organelles, i.e., Golgi apparatus, vesicles, lysosomes, etc., in the vicinity of bile canalicular region. 2. In the phalloidin or cytochalasin D treated groups, bile canaliculi were dilated and devoid of microvilli. In phalloidin treated group, the pericanalicular ectoplasm containing the microfilaments microfilaments was thicker than that of cytochalasin D treated group. Whereas, the dilation of bile canaliculus was more marked in cytochalasin D group. 3. Both drugs, phalloidin or cytochalasin D, produced the alteration of cell shaper to form cytoplasmic protrusions at the cell surface. In the phalloidin treated group, protusions were pedunculated, and the microfilaments were accumulatd at the narrow neck region. In cytochalasin D treated group, in contrast, no microfilament barrier was seen at the broad base of protrusion which exhibit direct continuity with the internal cytoplasm. 4. Numerous vesicles and vacuoles were formed near the cell surface and perianalicular cytoplasm in the treated groups, and later in culture they fused each other to form large intracellular space. Eventurally. this space was connected to the extracellular space or bile canaliculus. This experiment demonstrated that excessive accumulation or depletion of microfilaments induced by phallyoidein or cytochalasin D altered the cell shape and disturbed the vesicular transport of bile components into bile canaliculi. The results suggest that dysfunction of microfilaments may play an imprtant role in the impairement of canalicular contraction and the integrity of microfilaments is necessary for the billiary secretin as well as for the maintainance of the cell shape of hepatocytes.

汎-고양이 植物凝集素로 分類한 사람 血靑型에 關한 硏究

金成宰,文國鎭 고려대학교 의과대학 1989 고려대 의대 잡지 Vol.26 No.3

Since the first study of phytagglutinin (PHA) in 1908 there have been many reports on PHA. THe utilization of PHAs in the medical and medicolegal field is increasing, especially as lectins for the blood grouping, as mitogens for the artificial culture of human cells, and as determinants of the receptor specificity of various tumor cells. There are reports that human serum can be classified not only by agglutination activity to the animal red blood cells but by inhibition property to agglutination reaction of PHA to various red blood cells. In this study, to examine serologocal relationships of human serum types classified by agglutination activity to cat red blood cells and by inhibition properties to agglutination reaction of PHA with cat red blood cells, agglutination test, agglutination lnhibition test, and titration of agglutination inhibition were carried out with 948 human sera. The results could be summarized as follows; 1. The human sera were classified into two types according to agglutination activities to cat red blood cells. 2. The human sera were also classified into two types by the inhibition properties against agglitination of Dolichos lablab L. extracts and Celosia argrntea L. extracts to cat red blood cells. 3. It was dermonstrated that there is a close relationship between the serum types classified by agglutination activities to cat red blood cells and those classified by agglutination inhibition properties. 4. The sera of the inhibitor that inhibit the agglutination of pan-cat PHA were classified into two types according to the agglutination activity to the cat red blood cells. The finding in the non-inhibitor's sera were alike as well. 5. It was dermonstrated that the agglutination substance to cat red blood cells and agglutination inhibition substance to pan-cat PHAs in human sera are quite different to each other and exist independently.

식물응집소 Vicia pallida Turcz. 및 개 적혈구에 의한 사람 혈청형에 관한 연구

金鍾哲,文國鎭 고려대학교 의과대학 1987 고려대 의대 잡지 Vol.24 No.3

Some plant extracts are known to have biological activities as phytagglutinins (PHA's) and, in recent medicolegal studies, human sera were classified by their agglutination inhibiting properties to animal red blood cells. Vicia pallida Turcz. acts as pan-PHA to dog red blood cells. Therefore the author carried out agglutination inhibition test on Vicia pallida extract to dog red blood cells with 851 human sera, and the results are compared with known blood and serum types. The results are obtained as follows: 1. Human sera could be classified into two groups. One group inhibited agglutinating activities of vicia pallida extracts to dog red blood cells and the other did not. 2. The distribution of the former was 15% and the latter was 85%. 3. The agglutination inhibiting phenomena of human sera on Vicia pallida extract to dog red blood cells showed no constant relation with known blood and serum types such as ABO, MN, H, CI, Htr, PVI and CCI respectively. 4. This new classification of human sera is considered to be applicable to individual identification of blood stains.

植物凝集素 Rheum koreanum Nakai 및 토끼赤血球에 의한 사람 血淸型에 關한 硏究

金正珠,文國鎭 고려대학교 의과대학 1985 고려대 의대 잡지 Vol.22 No.3

In medicolegal field, various classifications of human materials, especially blood, have great importance in individual identification, paternity test and so on. Although numerous methods have been used for the classification of human blood, some plant extracts were also used because of their agglutinating activities on human red blood cells. Recently, plant extracts, which have panhemagglutinating activities on animal red blood cells, were used for new classification of human serum types by several frontiers. So, the author surveyed the change of the agglutinating activities of Rheum Koreanum Nakai(RKN) extract, which act as panphytagglutinin on rabbit red blood cells, by human sera. In this study, samples of 645 human sera, 152 serum stains and 134 whole blood stains were tested and the results could be summarized as follows: 1. Human, sera could be derided into two groups. One group inhibited the agglutinating activities of RKN extract on rabbit RBC, and the other didn't. 2. The distribution of the former was 7% and the latter was 93%. 3. This serum type showed no constant relation with known blood types or serum types such as ABO, H, Cl, Cea, ab. C, CCI, PVI. etc. 4. This new serum type may be available for the classification of human blood stains.

약물에 의해 미세사의 기능장애를 받은 배양간세포의 미세구조 변화

김진국,고정식,안의태,박경호 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.5

만성 신부전 환자에서 혈액투석에 의한 Isoniazid의 제거

신재국,차인준,장인진,신상구,김윤구,한진석,김성권 大韓臨床藥理學會 1994 臨床藥理學會誌 Vol.2 No.2

배양 간세포의 극성과 세포막의 분화에 대한 미세구조적 연구

김진국,양남길 대한해부학회 1995 Anatomy & Cell Biology Vol.28 No.2

韓國産 植物凝集素 Dolichos lablab L.의 凝集活性에 關한 硏究

金周弼,文國鎭 고려대학교 의과대학 1982 고려대 의대 잡지 Vol.19 No.3

The author used extracts from the seeds of Dolichos lablab L. native to Korea. Study on its hemagglutination activities to red blood cells of 1,183 peoples, agglutination with human red blood cells, agglutination, titration, agglutination inhibition with human saliva and various sugar solutions, absorption with human red blood cells and agglutination with ficinated red blood cells were carried out. The results could be summarized as follows; 1. By using Dolichos lablab extracts, two types were identified. One is agglutinated blood group designated as DL(+) type, and the other is non-agglutinated blood group designated as DL(-) type. 2. 2% of normal group was in DL(-) type. 3. Agglutination activity of Dolichos extracts was not inhibited by human saliva and various sugars. 4. After ficinization of red cells, agglutination activity of DL (+) type was increased. 5. DL blood type showed no connection with special diseases.