Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

대량의 각혈로 사망한 장티푸스 1예

한상우,유진홍,권순석,김성훈,박지찬,홍은정,최경성,박용완,장준희,안지원,박유경,강지영,박상미,신완식 대한감염학회 2004 감염과 화학요법 Vol.36 No.5

We experienced a 25 year-old male patient with typhoid fever complicated with massive hemoptysis. Pulmonary complication in typhoid fever is very rare and to our knowledge, there has been no report of hemoptysis as a main cause of death with this disease. We herein report a rare case of typhoid fever.

고출력 LED의 접합온도 측정회로 설계 및 구현

박종연,유진완 江原大學校 産業技術硏究所 2010 産業技術硏究 Vol.30 No.A

Recently, the LED lighting is widely used to illumination purpose due to its high luminous efficiency and the long life time. However, the light power and lifetime is reduced by junction temperature increment of LED. So it is important to measure the junction temperature accurately. In this paper, we proposed a new design and implementation method of high power LED junction temperature measurement circuit. The proposed circuit has two current sources which are a driving current source and a measurement is verified by experiment, and the result shows that the proposed circuit is appropriate to practical use.

아세톤-물 混合溶媒中의 브롬化페나실과 파라클로아닐린과의 反應速度硏究

朴鎭夏,洪性完 全北大學校 基礎科學硏究所 1982 基礎科學 Vol.5 No.1

The rate constants of the reactions of phenacyl bromide with p-chloroaniline in acetone-water at 30, 35 and 40℃ were determined by means of electric conductivity method and some activation parameters were also calculated. In the results, the rate constants were first order with respect to each reactant and increased with water content and the values of entropy of activation were quite negative and those of enthalpy of activation were comparatively low. Therefore, it is concluded that the reactions were to be S_N2 mechanism.

샌드위치 구조물의 형상최적설계

박철민,박경진,이완익 한양대학교 공학기술연구소 1992 공학기술논문집 Vol.1 No.1

General structural optimization is utilized to minimize the weight of structures while satisfying constraints imposed on stress, displacements and natural frequencies, etc.. Sandwich structures consist of inside core and outside face sheets. The selected sandwich structures are isotropic sandwich beams and isotropic sandwich plate. The face sheets are treated as membrane and assumed to carry only tensions, and the core is assumed to carry only the transverse shear. The characteristics of the varying area are considered by adding the projected component of the tension to the transverse shear. The bending theory and energy method are adopted for analyzing sandwich beams and plates, respectively. In the optimization process, the cost function is the weight of a structure, and a deflection and stress constraints are considered. Design variables are thickness and tapering coefficients which determine the shape of a structure. An existing optimization code is used for solving the formulated problems.

급성 파라쿼트 중독에서 혈중 파라쿼트 농도

박승민,김세현,최수진,김현,이완구,김영남,이광영,이영희,신성혜 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3

Background: Paraquat is a bipyridyl compound, and when ingested, concentrated paraquat can cause either rapid death from multisystem failure and cardiovascular shock or delayed death from progressive pulmonary fibrosis. Paraquat is poorly absorbed by inhalation, but when ingested orally, severe illness can occur. Death usually occurs within 2 days if more than 50 ㎎/㎏ of paraquat is ingested . The most important prognostic indicator is the quantity of paraquat absorbed, as shown by the plasma paraquat concentration, and the prognostic indication depends mostly on the description given by the patients and their families about the amount of paraquat ingested, which is often underestimated or overestimagted. For these reasons, we tried to compare the plasma paraquat concentrations with amount of paraquat described by patients or their families. Methods: We reviewed the medical records of 59 patients with acute paraquat poisoning from February 1998 through February 1999, the paraquat concentrations in plasma were measured at Presbyterian Medical Center by using high performance liquid chromatography. Results: There was a striking discrepancy between the plasma paraquat concentration and the ingested amount described by the patients or their families. Conclusion: We recommend that the plasma paraquat concentration be measured in patients being treated for acute paraquat poisoning.

"한국판 학령전기 기질성격검사[K-psTCI]" 의 신뢰도 및 타당도 검사

박진균,김지웅,기선완,신의진 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Objectives : The purpose of this study was to evaluate the reliability and validity of the Korean version of Preschool Temperament and Character Inventory (K-psTCI), a questionnaire based on Cloninger's seven-factor model of personality. Methods : The psTCI was translated into Korean and administered to 266 children aged 2-6 years. α test-retest study of the K-psTCI was conducted across a 4-month interval, Internal consistency was calculated by Cronbach a. Test-retest and reliability was analyzed by Pearson correlation analysis. Factor analyses for the temperament and character dimensions were performed using principal component analysis, rotating factors by varimax. The psTCI scores of Korean and United States preschoolers were compared Results : Cronbach α values for the K-psTCI scales ranged from .62 to .78 for each dimensions. Test-retest correlations (r) ranged from .50 to .77 for each dimensions. Explorative factor analysis with the condition of eigenvalue greater than 2 produced four factors for the temperament items and three factors for the character items like the US original version of psTCI. Through factor analyses, five items in the K-psTCI were categorized differently from the US version of psTCI. Korean preschoolers had higher mean scores on Cooperativeness (CO) in males as compared to a sample of US preschoolers. Conclusion : The results of this study suggests that the Korean preschool TCI is satisfactory in reliability and validity.

LED 기생 커패시턴스를 고려한 접합온도 측정 시스템의 개선

박종연,유진완 江原大學校 産業技術硏究所 2009 産業技術硏究 Vol.29 No.B

Recently, we have used LEDs to illumination because it has a high luminous efficiency and prolong lifespan. However the light power and lifetime is reduced by junction temperature increment of LED. So it is important to measure the junction temperature accurately. In case of using a electrical method measuring junction temperature of LED. Temperature measurement errors are spontaneously generated because of a parasitic capacitances in LED. In this paper, we proposed a method that reducing LED's parasitic capacitance effects for electrical measurement. It was demonstrated by the experimental result that is more correct than established method.

표고버섯의 저장성 연장에 관한 연구

박철,윤희진,신완철,최석영 울산대학교 2006 생활과학논문집 Vol.7 No.2

저농도의 산소를 함유한 기체조성(G108)과 고농도의 이산화탄소를 함유한 기체조성(G604)을 이용하여 표고버섯의 저장성 연장 효과를 평가하였다. 표고버섯을 실온(22°C)과 냉장저장(4°C)으로 나누어 저장하면서 중량감소율, pH 변화, 색도(L값), PPO 활성, 유리아미노산 함량을 측정하였다. 실온저장 대조군은 7일 경과 후 중량이 6.3%까지 감소하였으나, G108군과 G604군은 각각 3.0%, 3.2% 감소하였다. pH는 실온저장 대조군에서는 저장 3일째 급격히 감소하다가 7일에는 크게 상승하였다. 반면에 G108과 G604군에서는 pH 변화가 3일까지 변화가 없다가 7일에는 감소하였다. L값은 실온저장 3일에 대조군이 46.4인 반면, G108과 G6O4군은 7일에 각각 76.1, 76.6이었다. 냉장저장 시 대조군의 중량은 7일부터 감소하기 시작하여 35일에는 39.0%까지 감소하였으나, G108과 G6O4군에서는 42일까지 각각 3.1%, 3.0% 감소하였다. 대조군의 pH는 저장 21일까지 크게 감소하다가 증가하기 시작하여 저장 35일째에는 크게 상승하였다. 반면에 G108과 G604군에서는 저장 42일까지 pH변화가 거의 일어나지 않았다. 냉장저장 시 대조군의 L값은 14일 경과 후 큰 폭으로 떨어졌지만, G108군과 G6O4군은 42일까지 별로 감소하지 않았다. PPO 활성은 대조군에서는14일부터는 크게 증가하였으나 G108과 G604 모두 저장 42일까지 거의 증가하지 않았다또한 유리 아미노산 함량도 대조군에서는 크게 감소하였으나 기체치환군에서는 그 감소율이 크게 지연되었다. 이상의 결과로 표고버섯의 저장 시 기체치환으로 버섯의 호흡을 억제함으로써 버섯의 저장성을 연장시킬 수 있음을 알 수 있었다. 실온저장 시 대조군은 1일부터 각종 품질 변화가 일어나는 반면에, 기체치환저장은 3일까지 변화가 일어나지 않았으며, 냉장저장 시에는 대조군이 3일경부터 품질 변화가 일어나는데, 기체치환저장은 14일까지 품질의 큰 변화가 없음을 알 수 있었다. The prolongation of shelf-life of oak mushroom(Lentius edodes) using low oxygen concentration(G108) and high carbon dioxide concentration(G604) was evaluated. The weight loss, pH change, colour(L value), PPO activities and free amino acid contents were measured during 7 days at 22"C or during 42 days at 4'c storage. During 22"C storage the weight loss rate of control was 6.3% at 7 days, but that of G108 and G6O4 were 3.0% and3.2% at 7 days respectively. The pH of control was decreased at 3 days, but increased thereafter, However The pH of G108 and G6O4 were not changed during 3 days and were decreased thereafter. The colour (L value) of control was 46.4 at 3 days, but that of G108and G604 were 76.1, 76.6 during 7 days respectively. During 4"C storage the weigh loss rate of control was 39.0% at 35 days, but that of G108 and G6O4 were little changed during 42 days. The pH of control was decreased at 21 days, but increased after 35 days. The pH of G108 and G604 were not changed during 42 days. The colour (L value) of control was highly decreased at 14 days, but that of G108 and G6O4 were little changed during 42 days. Also, PPO activities of control were continually increased from 14 days, that of G108 and G6O4 however were little changed during 42 days. The contents of free amino acids of control were decreased continuously, but their decreasing rate was delayed in the groups of modified atmosphere packaging. These results suggested that the shelf-life of oak mushroom was prolonged by inhibiting the respiration of that using the modified atmosphere packaging. During 22'c storage the qualities of control were not changed at 1 days, but that of G108 and G6O4 were not changed during 3 days. Also during 4f storage the qualities of control were not changed at 3 days, but that of G108and G6O4 were not changed during 14 days.