Pulmonary inflammation caused by silica dioxide nanoparticles in mice via TXNIP/NLRP3 signaling pathway

Je‑Oh Lim,Je‑Won Ko,Tae‑Yang Jung,Woong‑Il Kim,So‑Won Pak,In‑Sik Shin,Won‑Kee Yun,Hyoung‑Chin Kim,Jeong‑Doo Heo,Jong‑Choon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Silica dioxide nanoparticles (SiONPs) have been used for various medical applications, including therapeutics and imaging, and the use of SiONPs has increased gradually over the years. However, despite an increase in the use of SiONPs, not much is known about mechanism of action of SiONPs and their pulmonary toxicity. Objective The present study investigated the pulmonary toxicity of SiONPs and explored the underlying mechanism of action, primarily focusing on thioredoxin-interacting protein (TXNIP)/NOD-like receptor pyrin domain-containing 3 (NLRP3) in SiONPs-treated mice. We investigated the toxic effects of SiONPs in the lung of BALB/c mice administered 5, 10, and 20 mg/kg SiONPs for 3 days. Results Exposure to SiONPs markedly increased inflammatory cell counts, including those of neutrophils and macrophages, and levels of inflammatory mediators, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α in a dose-dependent manner in the bronchoalveolar lavage fluid. Moreover, the inflammation was verified upon histopathological analysis. In addition, exposure to SiONPs increased the expression of TXNIP in a dose-dependent manner and, in turn, upregulated NLRP3 inflammasome proteins, which subsequently induced IL-1β production. Conclusion Collectively, exposure to SiONPs induced inflammation in the lungs of mice, which resulted in the activation of IL-1β production via the TXNIP-NLRP3 axis. Our results provide useful information on the pulmonary toxicity induced by SiONPs and provide insights into the underlying mechanism of action.

뇌하수체 종양 제거술 시행 후 뒤늦게 발생한 횡문근 융해증과 동반된 중추성 열

장제혁,최규남,김일환,노은지,김윤정,유충헌,고정해,박봉수,김태균,권민정,이순희,박정현 인제대학교 2008 仁濟醫學 Vol.29 No.-

Body temperature is controlled by thermoregulatory center of the hypothalamus. We report a case of 24 years old man with central fever. He was subjected to a partial excision of pituitary tumor compressing optic chiasm four years ago. He has received hormonal therapy for panhypothyroidism after removal of pituitary tumor. And He received gamma knife operation for partially contrast-enhancing masses in suprasellar and both hypothalamic areas that is probably postoperatively remnant or recurrent tumor of pituitary adenoma. One year after gamma knife operation, he presented with a febrile syndrome of unknown origin including rhabdomyolysis. All usual investigations proved negative. We diagnosed him as central fever with rhabdomyolysis. He received medical ICU care with cooling bed, ice pack. And his symptom improved. Postoperative hyperthermia may result following resection of the pituitary tumor. When central fever is suspected taking note of past history, a quick recognition of course of fever can help reduce the using of unnecessary antibiotics and hospital stay.

제어 시스템 설계를 위한 IEC 1131-3 기반의 제어 로직 생성기의 개발

정구,심주현,이제필,이철수 한국공작기계학회 2001 한국공작기계학회 춘계학술대회논문집 Vol.2001 No.-

This paper describes the methodology of an IEC 1131-3-based control logic generator for the control system design and converting algorithm between programmable languages. The proposed control logic generator is generated based on the software model and common element with data type, variables, POUs(program organization unit) and execution control unit commonly used within programmable languages of IEC 1131-3 Standard. The generation method of object file was proposed on five programmable language based on IEC1131-3. The generation method of object file is represented as following; 1) the generation method using conversion algorithm from LD to IL with FBD(function block diagram), 2) the generation method using C code generation algorithm from SFC using the SFC execution sequence with FBD and ST(structured text). The proposed control logic generator was implemented by Visual C++ and MFC on MS-windows NT 4.0

有機溶劑染色에 의한 PET Filament 의 染色性과 物性變化에 관한 硏究

鄭濟石,李相奎 단국대학교 1989 論文集 Vol.23 No.-

Poly(ethylene terephthalate) filament was dyed with C.I. Disperse Orange 3 in DMF, MCB, and PER dyebath at varying temperature in order to examine the dyeing properties related with the changes in the physical properties. The results of experiment were as follows; 1. Amount of dye uptake decreased in order of PER, MCB, and DMF, and diffusion coefficients of dye were increased according to increasing temperature, but amount of dye uptake were deacreased. 2. Shrinkage and degree of swelling in DMF which interacted with the aliphatic residue of PET were shown better than other solvents. 3. Dye exhausion rate increased the linear proportion with the change in the physical properties increased until the equilibrium dye uptake. 4. In isothermalic condition, degree of crystallinity was increased as time pass.

界面活性劑가 有機실리콘 第四級암모늄鹽의 抗菌性에 미치는 影響

鄭濟石,白欽吉 단국대학교 1987 論文集 Vol.21 No.-

After knitted cotton fabric was finished silicone quaternary ammonium salt of antibacterial agent, effects of treatment by nonionic and anionic surface active agent and salt-methanol rinsing on antibacterial properties were investigated. When laundered with anionic surface active agent, cation of quaternary ammonium, antibacterial functional radical from the first laundry was compete masked with anion of surface active agent, and antibacterial properties was decreased extremely after ten times of laundry. But, as a result of milk test, the effect of antibacterial properties was known as positive comparing with unfinished fabric.

발아과정을 통한 3종 식물의 내염성 단백질 합성

정숙주,우제창 木浦大學校基礎科學硏究所 1997 基礎科學硏究誌 Vol.15 No.-

염분을 농도별로 첨가한 배지에서 유채와 무 0.2%때 발아율이 가장 높았다. 염분의 농도별 생장율을 조사한 결과 유채와 무에서 0.4%와 0.6%의 염분이 첨가 되었을 때 생장이 가장 좋았다. 3종 작물을 각기 다른 염분내성 단백질을 생산해내는 것으로 나타나서 유채는 35kD와 26kD의 단백질을 새로 생산해 내었으며 무는 40kD와 16kD 그리고 벼는 0.4%에는 30kD, 고농도에서는 20kD의 단백질을 생산했다. Germination rate of Brassica and Raphanus was observed highest rate at the 0.2% NaCl concentration. Growth rate of Brassica and Raphanus showed maximum value at the 0.4% and 0.6% respectively. Salt resistant protein was biosynthesized with different molecular weight; Brassica synthesized 35kD and 26kD protein, Raphanus with 40kD and 16kD and rice produced 320kD and 20kD protein at the 0.4% and over 0.6% concentration of NaCl respectively.

하천 수질에 대한 36시간 연속 모니터링 기법 연구

박정규,정흥배,문성환,류태권,류제영,황인영 한국환경독성학회 2001 환경독성보건학회지 Vol.16 No.4

1. 금호강물에 대한 상대독성과 수온, 전기전도도의 경우 달서천 내부, 금호강과 달서천의 합류점 하부, 합류점 상부 순으로 값의 크기를 보였고 pH의 경우 역순을 보이므로 달서천이 금호강과 달서천 합류점 하부에 영향을 주는 것으로 사료된다. 2. 금호강 하천수에 대한 Microtox 독성값과 온도, pH, 전기전도도의 상관성은 없었으므로 기존의 일반적인 몇 가지 수질 인자로는 독성변화 및 수준을 판단할 수 없다고 사료된다. 3. 36시간 연속 모니터링을 통해 짧은 시간에도 독성값이 달라지는 것으로 보아 수질의 독성 수준이나 오염 수준을 판단하기 위해서는 연속적인 독성 조사 및 분석이 필요하다고 사료된다. 4. 하천수에 대한 연속 모니터링만으로는 하천 생태 위해도를 평가하기는 어렵다. 따라서, 하천 퇴적물에 대한 특성을 병행하여 조사 분석하는 것이 유해화학물질에 의한 하천 오염을 평가하는 방법이 된다고 사료된다. The main point source of pollution of the Keumho river in Taegu, Korea, stems from waste from the areas of industrial complexes. Although it is widely accepted that pollutants in waste water negatively effects general water quality, it is difficult to evaluate the effluent effect because of varying conditions in ambient water and inconclusive knowledge of causative pollutants. To analyze the water in relation to the industrial effluent in the area, pH, temperature, conductivity, and Microtox toxicity of various river samples were measured. Water samples were collected every 2 hours for 36 hours from Keumho river and Dalseo stream. Data from continuous monitoring for 36 hours showed that effluent in Keumho river originated from Dalseo stream, which is near adjacent to industrial complexes. Change in toxicity and other factors tested during the 36 hours indicated that continuous monitoring was necessary for a satisfactory effluent toxicity test. Furthermore, in addition to water quality monitoring, it was concluded that sediment toxicity also needed to evaluate effluent effects.

船體의 上下振動에 對한 附加質量計算에 附加質量3次元修正係數 分布法

金正濟 울산대학교 1977 연구논문집 Vol.8 No.2

선체의 상하진동에 대한 부가질량의 계산에 있어서 타원단면주상체의 부가질량 3차원수정계수를 배의 각단면에 분포시켜 계산하는 방법의 가능성 및 타당성을 검토하기 위하여 본 방법으로 실선에 대한 계산을 수행하였으며 현재통용되는 두가지 방법, 즉회전타원체의 단일 3차원수정계수 적용 방법 및 유한장 타원단면 주상체의 단일 3차원 수정계수 적용법에 의한 계산치들과 비교검토 하였다. 본 방법은 결과적으로 기존 방법에 비하여 진동수에는 큰 차이를 주지 않으나 보다 정확한 부가질량 계산방법으로서의 실용성이 있음을 확인 하였다. To investigate the possibility and reliability of Three-dimensinal correction factor distribution method for calculation of added mass of vertically vibrating ship, the Author has carried out some calculation of added mass for actually existing ship by this proposed method. The Author also has calculated the same by applying existing two methods, application of unique 3-dimensional correction factor of ellipsoid of revolution and application of unique 3-dimensional correction factor of finitely long elliptic cylinder, and investigated the former result in comparision with these values. The new proposed method was confirmed reasonable with more accuracy in calculation of added masseven though it does not give so signficant difference in vibration frequeney compared to existing methods.

진행성 비호지킨 림프종 환자에서 ICE 구제 요법에 의한 조혈모세포 가동화 후 자가 말초혈액 조혈모세포이식

이제중,이병환,김여경,변정래,이일권,박무림,정익주,김형준 대한조혈모세포이식학회 2003 대한조혈모세포이식학회지 Vol.8 No.1

연구배경: 비호지킨 림프종에서 자가 말초혈액 조혈모세포이식술이 폭넓게 이용됨으로 인해서, 높은 치료효과뿐만 아니라 조혈모세포의 가동화 효율이 높은 구제 요법이 요구되었다. 방법: 본 연구에서는 진행성 비호지킨 림프종에서 ICE 요법을 이용하여 조혈모세포를 가동화시킨 후 자가 말초혈액 조혈모세포이식이 시행하였던 환자를 후향적으로 분석하였다. 결과: 대상 환자의 중앙 연령은 38세(범위, 16~61)였으며, ICE 요법은 환자당 4주기(범위, 1~6주기)가 투여되었고, 투여 간격은 중앙값이 24일(범위, 16~36일)이었다. 고위험도 관해군을 제외한 13예의 환자 중 완전반응은 7예(53.8%), 부분반응은 4예(30.8%), 진행성질환은 2예(15.4%)를 보였다. 치료에 따른 3~4등급의 혈액학적 독성은 중성구감소증이 13예(81.3%), 혈소판감소증이 7예(42.8%)에서 관찰되었다. 조혈모세포 채집은 ICE 요법 후 중앙값이 12일(범위, 6~20일)에 시행되었고, 각 ICE 요법당 채집한 단핵구치는 중앙값이 5.75× 10^(8)/체중, CD34^(+) 세포는 중앙값이 1.25× 10^(6)/체중, CFU-GM치는 중앙값이 1.19× 10^(5)/체중이었다. 조혈모세포이식은 11예에서 시행되었고, 중앙 추적기간 401일에 평가한 2년 전체생존율은 70.1±14.7%, 1년 및 2년 무사건생존율은 각각 60.7±15.4%와 32.3±17.1%를 보였다. 결론: ICE 요법은 재발성/불응성 비호지킨 림프종 환자에서 높은 치료 반응률과 만족할 만한 조혈모세포 가동화를 보여 주었지만, 치료에 대한 순응도가 낮아서 투여 간격이 연장되는 문제점을 안고 있어서, 우리나라 환자의 실정에 적합한 구제요법에 대한 연구가 필요할 것으로 생각된다. Background: Due to the extensive application of high-dose chemotherapy with autologous peripheral blood stem cell transplantation (PBSCT), a salvage chemotherapeutic regimen with high response rate as well as effective capacity of PBSC mobilization is needed in non-Hodgkin's lymphoma (NHL). Methods: We analyzed the applicability of ICE (ifosphamide, carboplatin, and etoposide) regimen in NHL patients who underewent autologous PBSCT. Results: The median age was 38 years (range, 16~61 years), the patients received median 4 cycles (range, 1~6 cycles) of ICE regimen, and the median interval between each chemotherapeutic cycle was 24 days (range, 16~36 days). There were 7 (53.8%) complete responses, 4 (30.8%) partial responses, and 2 (15.4%) progressive diseases after ICE regimen. Toxicity included grade 3/4 neutropenia and throm bocytopenia in 13 (81.3%) and 7 (42.8%) patients, respectively. PBSC collection began on median day 12 (range, 6~20 days) after ICE therapy. The median number of mononuclear cells, CD34+ cells, and CFU-GM was 5.75× 10^(8)/kg, 1.25× 10^(6)/kg, and 1.19× 10^(5)/kg, respectively. With a median follow- up of 401 days, the patients who underwent autologous PBSCT had overall survival with 70.1±14.7% at 2 year and event free survival with 60.7±15.4% and 32.3±17.1% at 1 year and 2 years, respectively. Conclusion: ICE chemotherapy is an effective cytoreduction and mobilization regimen in patients with NHL, but profound myelosuppression with delayed recovery might pose difficulties in applying for Korean patients. Further evaluation for appropriate salvage regimens in Korean patients should be needed.

일제 식민지기 경성부 교외 지역의 전차 문제와 지역 운동 : 1932~33년 전차 교외선 폐지 반대 운동을 중심으로

김제정 서울시립대학교 부설 서울학연구소 2007 서울학연구 Vol.- No.29

The movement against the abolishment of the electric cars' outskirts line, which continued during 1932 and 1933, was a local movement originated from the outskirts area of the Gyeongseong-bu. This movement was based upon the dual structure of the Gyeongseong area and also the interests of the majority of the local residents, and continued for quite some time. The movement also involved, albeit partially, a nationalist agenda, and therefore was supported by the Joseon community as well. The movement proceeded in the most typical fashion we can observe from the Japanese occupation period. The issues were originated from the local resident leaders' meetings, then further discussed in conferences between representatives of Dong·Ri units, and then a movement community was organized to raise money and propagate their issues, and such efforts eventually resulted in the commencement of resident campaigns and submission of appeals. This movement was a rare success compared to other movements that were attempted under the colonial ruling. Although the movement featured one big flaw of having the colonial authorities involve themselves in the movement, the local movement itself was another opportunity of forming a public consensus nonetheless. And the concept suggested for the cause of the movement was 'public interest'. At the time, terms like 'public interest' or 'public matter' meant 'the interest of the majority', and in local movements, these terms meant the 'common interest of the loceil residents'. Electric cars were public assets that were usually used by all the members of local communities, so such assets always had the potential to generate a region-wide issue of common interest regardless of social classes or nationalities. In case of this electric car issue, 'safety and conveniences', 'protection of existing rights', 'local developments' were raised as reasons why such movement was necessary. And compared to this 'public interest' in the issue involving electric cars, the private(私) interest was known to be represented by a civilian company narned the Gyeongseong Electricity corporation. There was a social perceptive that civilian cornpanies in charge of public-natured businesses were required to serve public interests even such serving would limit the companies' pursuit of private interests or their excercise of property rights. The movement was led by local influentials, and also the collaborators of the colonial authorities. They maintained their cause of public interest while also holding onto their private profits, and the cause of 'local development' was what made that possible. This 'local development' cause was a social and economical basis which legitimatized the concept of collaborating with the colonial ruling, and the public's being assimilated into the occupation system.