http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Je‑Oh Lim,Je‑Won Ko,Tae‑Yang Jung,Woong‑Il Kim,So‑Won Pak,In‑Sik Shin,Won‑Kee Yun,Hyoung‑Chin Kim,Jeong‑Doo Heo,Jong‑Choon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3
Background Silica dioxide nanoparticles (SiONPs) have been used for various medical applications, including therapeutics and imaging, and the use of SiONPs has increased gradually over the years. However, despite an increase in the use of SiONPs, not much is known about mechanism of action of SiONPs and their pulmonary toxicity. Objective The present study investigated the pulmonary toxicity of SiONPs and explored the underlying mechanism of action, primarily focusing on thioredoxin-interacting protein (TXNIP)/NOD-like receptor pyrin domain-containing 3 (NLRP3) in SiONPs-treated mice. We investigated the toxic effects of SiONPs in the lung of BALB/c mice administered 5, 10, and 20 mg/kg SiONPs for 3 days. Results Exposure to SiONPs markedly increased inflammatory cell counts, including those of neutrophils and macrophages, and levels of inflammatory mediators, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α in a dose-dependent manner in the bronchoalveolar lavage fluid. Moreover, the inflammation was verified upon histopathological analysis. In addition, exposure to SiONPs increased the expression of TXNIP in a dose-dependent manner and, in turn, upregulated NLRP3 inflammasome proteins, which subsequently induced IL-1β production. Conclusion Collectively, exposure to SiONPs induced inflammation in the lungs of mice, which resulted in the activation of IL-1β production via the TXNIP-NLRP3 axis. Our results provide useful information on the pulmonary toxicity induced by SiONPs and provide insights into the underlying mechanism of action.
Refractory pseudocyst of auricle treated with surgical deroofing and tie-over dressing
( Won Ku Lee ),( Jeong Min Kim ),( Gun Wook Kim ),( Je Ho Mun ),( Margaret Song ),( Hyun Chang Ko ),( Byung Soo Kim ),( Moon Bum Kim ),( Hoon Soo Kim ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.1
Pseudocyst of the auricle (PCA) is characterized by asymptomatic dome shaped cystic swelling on the anterior surface of the auricle and caused by an intracartilaginous accumulation of viscous fluid. Despite various therapeutic approaches, it relapses frequently. A 66-year-old man presented with a 2-month history of an asymptomatic nodule on the right ear. Physical examination revealed a skin colored, fluctuant swelling on the scaphoid. Needle aspiration of the swelling yielded approximately 1ml of yellowish viscous fluid. With the diagnosis of PCA, sclerotherapy with sodium tetradecyl sulfate and minocycline had been performed, but the swelling appeared within few days after each attempt. Then, we tried multiple holing with 1mm punch biopsies and pressure dressing, however, the fluctuant swelling recurred. And so, we finally did surgical deroofing and tie-over dressing. The procedure was composed of resection of the anterior cartilaginous leaflet of the cyst and repositioning of the overlying skin flap followed by tie-over dressing. The lesion disappeared with complete adhesion between the skin and the cartilage after 1 week. The lesion has not recurred during 10 months of follow-up. We suggest that surgical deroofing followed by tie-over dressing is excellent surgical option of PCA as it is known with very less rate of recurrence, gives a cosmetically acceptable result, and can be easily done by dermatologists.
Genipin inhibits allergic responses in ovalbumin-induced asthmatic mice
Ko, Je-Won,Shin, Na-Rae,Park, Sung-Hyeuk,Cho, Young-Kwon,Kim, Jong-Choon,Seo, Chang-Seob,Shin, In-Sik ELSEVIER 2017 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.53 No.-
<P><B>Abstract</B></P> <P>Genipin is a natural compound isolated from the fruit of <I>Gardenia jasminoides</I> with various pharmacological effects. In this study, we investigated whether genipin effectively alleviates allergic responses in a murine model of ovalbumin (OVA)-induced asthma. The mice were administered an intraperitoneal injection of OVA on day 0 and 14 to boost the immune response; genipin was then administered from day 18 to 23 by oral gavage. On days 21 to 23, mice were OVA-challenged using am ultrasonic nebulizer, and airway hyperresponsiveness (AHR) was determined on day 24 by plethysmography. Genipin significantly reduced the inflammatory cell count in bronchoalveolar lavage fluids (BALF) and AHR, which were accompanied by lower interleukin-5 (IL-5), IL-13 and OVA-specific immunoglobulin (Ig) E levels in the BALF or serum from OVA-induced asthmatic mice. In histology, genipin significantly decreased airway inflammation and mucus hypersecretion in OVA-induced asthmatic mice. Additionally, genipin inhibited OVA-induced increases in the expression of inducible nitric oxide synthase and cyclooxygenase-2 proteins. Further, genipin reduced the activity and protein levels of matrix metalloproteinase-9 in lung tissue from OVA induced asthmatic mice. Overall, genipin effectively alleviated the asthmatic inflammatory response in an OVA-induced asthmatic model. Therefore, our results suggest that genipin has therapeutic potential for treating asthma.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Effects of genipin on ovalbumin (OVA)-induced asthmatic mice </LI> <LI> Genipin decreased airway hyperresponsiveness and eosinophilia in asthmatic mice </LI> <LI> Genipin reduced IL-5, IL-13 and OVA-specific IgE in asthmatic mice </LI> <LI> Genipin suppressed airway inflammation and mucus production in asthmatic mice </LI> <LI> Genipin inhibited iNOS, COX-2 and MMP-9 in lung tissue from asthmatic mice </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Je-Won Ko,In-Chul Lee,Sung-Hyuk Park,Changjong Moon,Seong-Soo Kang,Sung-Ho Kim,Jong-Choon Kim 한국실험동물학회 2014 Laboratory Animal Research Vol.30 No.4
We investigated the protective effects of pine bark extract (pycnogenol<SUP>®</SUP>, PYC) against cisplatin-induced hepatotoxicity and oxidative stress in rats. Twenty-four male rats were divided into the following four groups: (1) vehicle control, (2) cisplatin (7.5 mg/kg), (3) cisplatin & PYC 10 (10 mg/kg/day), and (4) cisplatin & PYC 20 (20 mg/kg/day). A single intraperitoneal injection of cisplatin induced hepatotoxicity, as evidenced by an increase in serum aminotransferase and histopathological alterations, including degeneration/necrosis of hepatocytes, vacuolation, and sinusoidal dilation. In addition, an increase in the malondialdehyde (MDA) concentration and a decrease in the reduced glutathione (GSH) content and catalase (CAT), superoxide dismutase (SOD), and glutathione S-transferase (GST) activities were observed in the cisplatin-treated rat hepatic tissues. In contrast, PYC treatment effectively prevented cisplatin-induced hepatotoxicity, including the elevation of aminotransferase and histopathological lesions, in a dosedependent manner. Moreover, PYC treatment also induced antioxidant activity by decreasing MDA level and increasing GSH content and SOD and GST activities in liver tissues. These results indicate that PYC has a protective effect against acute hepatotoxicity induced by cisplatin in rats, and that the protective effects of PYC may be due to inhibiting lipid peroxidation and increasing antioxidant activity.
P050 : Dermoscopic patterns of m alignant melanomas on the trunk and extremities in Koreans
( Je Ho Mun ),( Woo Il Kim ),( Gun Wook Kim ),( Hyun Ho Cho ),( Won Jeong Kim ),( Margaret Song ),( Hoon Soo Kim ),( Hyun Chang Ko ),( Byung Soo Kim ),( Moon Bum Kim ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2
Background: Dermoscopy has been proven to increase diagnostic accuracy of malignant melanomas. However, dermoscopic features of melanomas in Asians have been scarcely reported. Objectives: To investigate dermoscopic patterns and evaluate diagnostic algorithmic approach in Korean patients with melanomas in trunk and extremities. Methods: Dermoscopic evaluation of histopathologically confirmed melanomas at 2 university hospitals from 2007 to 2014 was performed. Dermoscopic scores were evaluated using various dermoscopic algorithmic methods. Results: Sixteen patients with 16 primary melanomas and 5 patients with 15 metastatic melanomas were included. In primary melanomas, several dermoscopic melanoma-associated features were found: blue-white veil (76%), blotches (62%), atypical dots/globules (52%), ulcer (48%), atypical pigment network (43%), irregular peripheral streaks (43%), atypical vessels (38%), shiny white lines (29%), and regression (10%). Mean scores of 7-point checklist, revised 7-point checklist, 3-point checklist, ABCD rule, and CASH algorithm were 6.3, 4.1, 2.6, 7.6, and 10.9. In metastatic melanomas, the algorithmic approaches were not accurate as the mean scores were 1.7, 0.9, 0.8, 2.0, 4.1. Conclusion: Dermoscopy can be useful in diagnosingmalignant melanomas in trunk and extremities in Koreans. Dermoscopic algorithmic approaches were useful in detecting primary melanomas; however, they were not precise in identifying metastatic melanomas.
Ko, Je-Won,Park, So-Won,Shin, Na-Rae,Kim, Woong-Il,Kim, Jong-Choon,Shin, In-Sik,Shin, Dong-Ho Korean Association for Laboratory Animal Science 2018 Laboratory Animal Research Vol.34 No.3
<P>Benign prostate hyperplasia (BPH) is a male reproductive disease that has gained increasing importance in recent years. The present study investigated whether Pycnogenol® (PYC), a standardized French maritime pine bark extract, could prevent BPH induced by testosterone propionate (TP) in rats. Male Sprague-Dawley rats were randomly divided into five groups of six rats. One group was used as a normal control rats and the other groups received subcutaneous injections of TP for 4 weeks to induce BPH. In the two treatment groups, PYC (20 or 40 mg/kg) was administered daily for 4 weeks by oral gavage concurrently with the induction of TP. All rats were sacrificed at the scheduled termination time, the prostates were weighed, and histopathologic examinations were conducted. Dihydrotestosterone (DHT) levels in serum and the prostate were measured, and the expression of proliferating cell nuclear antigen (PCNA) and Ki-67 proteins was investigated. BPH-treated animals showed increases in the relative weight of the prostate, higher concentrations of DHT in serum and the prostate, and higher expression of PCNA and Ki-67 in the prostate; in contrast, PYC-treated animals had significant reductions in these factors compared with the BPH animals. These findings indicated that PYC inhibited the development of BPH and that this was closely associated with a reduction in DHT concentration.</P>
P442 : Melanoacanthoma with calcification in patient with arsenic intoxication: their relationship?
( Won Ku Lee ),( Gun Wook Kim ),( Hyun Ho Cho ),( Won Jeong Kim ),( Je Ho Mun ),( Margaret Song ),( Hyun Chang Ko ),( Byung Soo Kim ),( Moon Bum Kim ),( Hoon Soo Kim ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.1
P212 : The impact of Vitamin D seems to be overestimated in some dermatoses
( Won Jeong Kim ),( Min Young Park ),( Gun Wook Kim ),( Hyun Ho Jo ),( Je Ho Mun ),( Margaret Song ),( Hoon Soo Kim ),( Hyun Chang Ko ),( Byung Soo Kim ),( Moon Bum Kim ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2
Background: Deficiency of vitamin D is reported as an important associated factor of various dermatoses such as atopic dermatitis, psoriasis, urticaria, and skin cancers in these days. However, the association between them is disputable and has not been clarified. Objectives: To evaluate the status of serum 25-hydroxyvitamin D in patients with psoriasis and chronic urticaria, and the relationship between vitamin D levels and disease activity compared with sex and age matched healthy control. Methods: A cross-sectional study of 34 patients with psoriasis, 73 patients with chronic idiopathic urticaria and sex and age matched healthy controls was conducted. An objective severity scoring of psoriasis (PASI) and urticaria (UAS) and a serum 25-hydroxyvitamin D level was measured for each subject. Results: Serum 25-hydroxyvitamin D levels in patients with psoriasis were not significantly lower than healthy control (P>0.05). The patients with chronic idiopathic urticaria also showed no significant differences compared with control subjects. Furthermore, no significant inverse correlation was found between disease severity and serum 25-hydroxyvitamin D level in both psoriasis and chronic idiopathic urticaria patients (P>0.05). Conclusion: Deficiency of vitamin D in patients with psoriasis and chronic idiopathic urticaria was not common than healthy control. The impact of vitamin D in these dermatoses seems to be overestimated and needs more study to prove its real association.