이종 유전자에 의한 형질전환된 담배의 광합성 능력회복

우제창 木浦大學校基礎科學硏究所 1996 基礎科學硏究誌 Vol.14 No.-

완두의 rbcS 유전자가 삽입된 pAC1350 vector 에 hygromycin transfere 유전자를 선별지표 (selector marker) 로 재삽입하여 p35SSU-HPH vector을 구성하고 이를 이미 antisence 유전자에 RUBISCO small subunit 유전자 발현이 억제된 식물체에 형질전환시켜 재분화를 유도하고 계속적으로 배양하여 그 종자를 채종하였다. 채종된 종자는 발아시 hygromycin이 함유된 배지에서도 16% 정도는 생장이 가능하였고, Southern 과 Northern bolt hybridization 의 결과 HPH 유전자를 핵내에 보유하고 있으며 완두의 rbcS유전자의 전사도 이루어지는 것으로 확인되었다. 이들을 생장속도에 따라 대, 중, 소로 구분하여 Southern bolt hybridization을 수행한 결과 모식물체 (parental plant)에서 보호하고 있는 4개 이상의 antisence 유전자는 0 - 4개의 band로 검출되어 생장이 좋을수록 antisence 유전자의 copy 수가 적은 것으로 확인되었다. 이들 종자의 광합성 능력은 모식물체와 정상 담배 식물체(SRI)의 중간을 나타내어 완두의 rbcS 유전자가 종자를 통하여도 전달됨을 보였고 이들 식물체의 세포내 CO₂ 농도는 400 μbar 정도로 일정하게 유지되어 삽입된 유전자에 의해 외부로부터 CO₂를 흡수하는 능력이 향상된 것으로 나타났다. RUBISO (Ribulose bisphosphate carboxylase/oxygenase) is sharply reducd in transgenic tobacco carrying and rbcS antisense(Rodermel et al., Cell 55:673-681/ 1988):expression of most if all member of the tobacoo rbcS family is reduced. To replace the tobacco rbcS gene products, plants with the tobacco antisense gene were transformed with T-DNA containing a 35S cauliflower mosaic virus promoter : pea rbcS gene and the hygromycin phosphotransferase gene. Such transgenic plants accumulated more RUBISCO small subunit protein than the parental strain and their photosynthetic activity was enhanced up to 150%. Progenies of selfed "double- transgenic" plants showed similar, though variable, patterns of hygromycin resistance and enhanced photosynthetic activity.

Chlamydomonas reinhardtii 엽록체 DNA의 형질전환

우제창 木浦大學校 基礎科學硏究所 1992 基礎科學硏究誌 Vol.10 No.-

pCrc44-rbcL-AAD, consist of rbcL promotor and aminoglycoside adenyl transferase (aadA) structural gene in atpB region of Chlamydomonas reinhardtii chloroplast DNA, was transformed to Chlamydomonas reinhardtii chloroplast by microprojectile bombardment. 37 clonies were selected as photosynthesis competent cell line on HS minimal medium, among them, 12 clonies showed spectinomycin resistant as a result of expression of aadA. All transformed cell line showed two distinctivly hybridized bands,4.1kb and 3.1kb, by using 0.5kb atpB fragment for probe. It meant that all of them were heterokaryon of normal and aadA transformed cells.

CHINESE BANKING REFORM OF 1998-2000 AND ITS EFFECT ON THE DEVELOPMENT OF A COMMERCIAL CREDIT CULTURE

우제창 연세대학교 동서문제연구원 2002 Global economic review Vol.31 No.1

1999년도 한국학술진흥재단 사업내용 및 향후 계획

우제창 목포해양대학교 해사산업기술연구소 1999 심포지움강연논집 Vol.1999 No.11

Chlamydomonas Reinhardtill rbcL Promoter 의 형질전환을 통한 atpB 부위에서의 발현양상

우제창,박영목 한국유전학회 1993 Genes & Genomics Vol.15 No.4

pCrc44 - rbcL - AAD, consist of rbcL Chlamydomonas reinhardtii chloroplast DNA and aminoglycoside adenyl transferase promoter from (aadA) structural gene from E. coli in C. reinhardtii chloroplast DNA atpB region, was transformed to atpB region of C. reinhardtii chloroplast DNA by microprojectile bombardment. 37 clonies were selected as photosynthesis competent cell lines on HS minimal medium, among them 5 clonies expressed photosynthesis competent and spectinomycin resistant as a result of expression of atpB and aadA genes. All transformed cell lines showed two distinctively hybridized bands, 4.lkb and 3.lkb, by using 0.5kb atpB fragment for probe. It meant that all of them were heterokaryon of normal and aadA transformed cells. RNA gel blot analysis showed that expression of rbcL promotor was inhibited during light illumination.

黃酸處理 및 溫度가 풍선덩굴의 種子發芽에 미치는 影響

梁孝植,禹濟昌 木浦大學校沿岸生物硏究所 1991 沿岸生物硏究 Vol.8 No.1

황산(98%) 처리시간과 온도가 풍선덩굴의 종자발아에 미치는 영향을 조사한 결과는 다음과 같다. 종자의 발아를 촉진시키는 효과적인 황산처리시간은 30분이었다. 발아율과 발아세는 30℃, 30분간의 황산처리구에서 각각 100% 34.17로 다른 처리구에 비해 가장 높았으며, 황산처리에 의한 발아율의 증가비는 다른 온도구에 비해 낮은 온도구에서 더 효과적 이었다. The seed germination were experimented according to period of sulfuric acid treatment at different temperature condition for Cardiospermum halicacabum L. The highest values of germination rate and velocity of seed were 100% and 34.10, respectively at 30℃ in 30 minutes' treatment with sulfuric acid. The effects of sulfuric acid treatment on seed germination was more increased in low temperatures' than in high.

발아과정을 통한 3종 식물의 내염성 단백질 합성

정숙주,우제창 木浦大學校基礎科學硏究所 1997 基礎科學硏究誌 Vol.15 No.-

염분을 농도별로 첨가한 배지에서 유채와 무 0.2%때 발아율이 가장 높았다. 염분의 농도별 생장율을 조사한 결과 유채와 무에서 0.4%와 0.6%의 염분이 첨가 되었을 때 생장이 가장 좋았다. 3종 작물을 각기 다른 염분내성 단백질을 생산해내는 것으로 나타나서 유채는 35kD와 26kD의 단백질을 새로 생산해 내었으며 무는 40kD와 16kD 그리고 벼는 0.4%에는 30kD, 고농도에서는 20kD의 단백질을 생산했다. Germination rate of Brassica and Raphanus was observed highest rate at the 0.2% NaCl concentration. Growth rate of Brassica and Raphanus showed maximum value at the 0.4% and 0.6% respectively. Salt resistant protein was biosynthesized with different molecular weight; Brassica synthesized 35kD and 26kD protein, Raphanus with 40kD and 16kD and rice produced 320kD and 20kD protein at the 0.4% and over 0.6% concentration of NaCl respectively.

담배에 형질발현된 완두 rbcS GENE의 발현

박승희,우제창 木浦大學校 基礎科學硏究所 1995 基礎科學硏究誌 Vol.13 No.-

Using a binary vector system with pea rbcS gene and hygromycin phosphotransferase gene for selectable marker, tobacco plant was transformed by means of a leaf disc transformation method. After co-cultivation, bacterial cells were removed by cefotaxim treatment. Transformed shoots were developed from the co-cultivated leaf disc within 1 to 2 weeks in selection media supplemented with 25㎍/㎖ hygromycin and 50㎍/㎖ kanamycin. While normal plants could not grow in the medium with 25㎍/㎖ hygromycin the transformed plants could survive in the medium with 200㎍/㎖ hygromycin. It meant that resistant gene and rbcS gene was expressed in regenerated tobacco plants. Differential protein accumulation of RUBISCO small subunit was not detected by SDS-PAGE.

尖察山의 植生에 關한 植物社會學的 硏究

金喆洙,禹濟昌 木浦大學校沿岸生物硏究所 1991 沿岸生物硏究 Vol.8 No.1

多島海 海上國立公園內의 鳥島群島의 森林에 對한 植生調査는 1989年 6月 1日에서 1989年 8月 31日에 걸쳐 Braun-Blanquet(1964)의 방법으로 實施하였다. 첨찰산의 植生은 1個의 植物群集과 10個의 植物群落으로 區分되었다. 常綠闊葉樹林인 구실잣밤나무-자금우 群集, 붉가시나무-참가시나무 群落, 동백나무 群落, 落葉闊葉樹林인 졸참나무 群落, 굴참나무 群落, 상수리나무 群落, 개서어나무 群落, 소사나무 群落, 常綠針葉樹인 곰솔 群落, 이대 群落, 억새 群落 等으로 分類되었다. 現在植生圖는 植生分類單位와 相關을 基準으로 하여 常綠闊葉樹林인 구실잣밤나무-자금우 群集, 붉가시나무-참가시나무 群落, 동백나무 群落, 落葉闊葉樹林인 졸참나무 群落, 굴참나무群落, 상수리나무 群落, 개서어나무 群落, 소사나무 群落, 常綠針葉林인 곰솔 群落, 草地 이대 群落, 억새 群落 等 11個 單位로 區分하여 제작하였다. Mt. Chomch'al(127˚17′30″-127˚20′00″E and 34˚27′30-34˚30′00″N), was covered with substitutional vegetation according to various human impact such as grazing, cutting for firewood. Phytosociological investigation of this forest vegetation was carried out by Braun-Blanquet's method from June to August, 1989. The forest vegetation was classified into 1 association, Ardisio-Castanopsietum sieboldii and 10 communities, Quercus salicina-Quercus acuta, Camellia japonica, Quercus variabilis, Quercus serrata, Quercus acutissima, Carpinus tschonoskii, Carpinus coreana, Pinus thunbergii, Pseudosasa japonica and Miscanthus sinensis var. purpurascens community. Based on the above classification, the actual vegetation map was drawn in 1:25,000 scale.

노회(Aloe arborescens)와 샤본노회(Aloe saponaria)의 핵형분석

禹濟昌,金美漢 木浦大學校基礎科學硏究所 1987 基礎科學硏究誌 Vol.5 No.-

For the purpose of the distinctive cytological differences between Aloe arborescens and A. saponaria, the karyotypes of two species were investigated. The results are as follows; 1. The somatic chromosome numbers of two species were found to be 2n=14. 2. The chromosomes of two species were composed of 8 long chromosomes and 6 short chromosomes. 3. The satellites were found from both species.