CdSe ; Cu 광전도 cell 제작

신영진,정태수,신현길,김택성,정철훈,이훈,신영신 경북대학교 센서기술연구소 1992 센서技術學術大會論文集 Vol.3 No.1

SWAT 모형을 이용한 태화강유역 장기유출 분석

신현석(Shin Hyun Suk),강두기(Kang Du Kee),김균태(Kim Kyun Tae) 대한토목학회 2004 대한토목학회 학술대회 Vol.2004 No.10

Vincristine Sulfate가 Mouse 모낭세포의 미세구종에 미치는 영향에 관한 전자현미경적 연구

신현태,이규식,류재만 한양대학교 의과대학 1990 한양의대 학술지 Vol.10 No.1

Vincristine sulfate, one of thte Vinca alkaloids has been used clinically as an antitumor reagent. Vincristine sulfate has high affinity to the basic protein subunit of microtubules, that it disrupts the mitotic spindle apparatus. As a result this reagent causes the cell to arrest in metaphase and to die finally. When vincristine was spread on the skin it would damage the growth of the hair, that the author undertook the present study to observe the effect of vincristine onthe cytoplasmic organelles of the hair follicular cells. Male albino mouse, DDY strain, weighing 20gm were used as an experimental animals. The back skin of experimental animals were spread with a vincristine sulfate solution(1mg/5ml) once a day for 6 days after cutting of the hair. The experimental animals were sacrificed at 2 day, 4 day, and 6 day after first treatmetn and specimens obtained from the skin of the vincristine treated area. The specimens were prefixed in 2% glutaradehyde-2.5% paraformaldehyde solution and postfixed in 1% osmium tetroxide solution and then ultrathin section, 600-800A, were made and stained with uranyl acetate and lead citrate. Some specimens were also fixed in 10% formalin for hematoxylin eosin stain. The specimens were observed with light microscopy and JEM 100cx-II electrom microscopy. The results were as follows. 1. Hair follicles were decreased numerously in the skin of the 4th and 6th vincristine treated group. And the length of the hair was reduced in the skin of the 6th vincristine treated group. 2. In the 2nd vincristine treated group, irregularly shaped nuclei, reduced heterochromatin and numerous vacuoles wre observed in the external root sheath cells. In cells of the inner root sheath, heterochromatin was segregated and clumpped, and cristae of mitochondria was disappeared. 3. In the 4th vincristine treated group, dilatation of cisternae of the rough endoplasmic reticulum, degenerated mitochondria were observed in the external root sheath cells. And trichohyaline granules were reduced and condensed nuclei were observed in the cells of the Henley's layer. 4. In the 6th vincristine treated group, cuticle cells were more keratinized. And in the external root sheath cells, colummar shaped nuclei, degenerative mitochondria and rough endoplasmic reticulum, and decreased cisternae of Golgi apparatus were observed. Consequently, it is suggested that vincristine sulfute would induce the degenerative changes in the hair follicular cells.

Streptomyces 속 균주 HA-40이 생산하는 내열성 α-Amylase의 효소학적 성질

신운섭,이동희,이현우,권태종,정호권 建國大學校 附設 産業技術硏究所 1988 논문집 Vol.13 No.-

토양으로부터 분리한 Streptomyces 속 균주 HA-40이 생산하는 내열성 α-amylase의 효소학적 성질을 조사한 결과는 다음과 같다. 효소의 작용 최적 온도는 65℃였으며 activation energy 는 16.9Kcal/mole이었고 60℃에서는 안정하였으나 70℃에서는 30분 처리로 약 50%, 1시간 처리로 약 75%가 실활하였다. 그러나 ??를 0.1mM농도로 첨가한 경우엔 70℃ 에서 1시간 처리하여도 잔존활성도가 55%이상으로 열변성에 대한 ??의 보호효과가 있었다. 본 효소는 pH7에서 활성이 가장 강하였으며 안정성도 가장 높았다. 그리고 금속이온 중에서 ??의 첨가로 효소활성이 증대되었으며 ??, ??, ??등에 의해서 활성이 저해되었다. 각종 효소저해제는 대부분 영향을 미치지 못하였으나 EDTA가 반응액 중에 공존하면 효소활성이 강하게 저해되었다 본 효소는 maltotriose이하의 당류에 대해서는 분해력이 없었으며 amylose에 대한 환원당 생성력을 100으로 하였을때 soluble starch 108, amylopectin 94, glycogen 87, dextrin 48, potato starch 25, corn starch 18로 생전분에 대한 분해력은 soluble starch에 비해서 1/4∼1/5 정도밖에 되지 않았다. 그리고 soluble starch에 대한 Km 치는 1.9㎎/㎖였다. The properties of a thermostale α-amylase produced from Streptomyces sp. strain HA-40, isolated from soil, were investigated. The optimal temperature of the enzyme was 65℃ and the enzyme was stable below 60℃. However, at 70℃ about 75% of the activity was lost after 1hr treatment, but by the addition of ?? in the concentration of 0.1mM only 45% of the activity was lost. The enzyme was most active and stable at around pH 7. The activity of the enzyme was increased by the addition of ??, but depressed by ??, ??, and EDTA. The enzyme could be hydrolyze soluble starch, amylose, amylopectin and glycogen well, and could also digest dextrin and raw starch. The Km value for soluble starch was 1.9㎎/㎖.

Fluoranthene 독성에 기인하는 비정상적 어류행동의 신경생화학적 분석

신성우,조현덕,전태수,김정상,이성규,고성철 한국환경독성학회 2003 환경독성보건학회지 Vol.18 No.2

Fluoranthene, a common polycyclicaromatic hydrocarbon (PAH), exhibits phototoxicity which may affect aquatic organisms, The eventual goal of this study is to develop a biomarker of Japanese medaka (Oryzias iatipes) used in monitoring hazardous chemicals in the ecosystem. In this study we investigated neural toxicity of fluoranthene in Japanese medaka (Oryzias latipes) along with comparative analysis of corresponding behavioral responses. The untreated individuals showed normal behavioral characteristics (i.e' smooth and linear movements). The treated fish, however, showed stopping and abrupt change of orientation (100 ppb), and severely reduced locomotive activity and enhanced surfacing activity (1,000ppb). Treatment of the medaka fish with fluoranthene caused a significant suppression of acetylcholine esterase (AChE) activities in the body portion but not in the head portion. When fish were exposed to 1,000pph of fluoranthene for 24hr, the body AChE activities decreased from 126.±31.89 (nmoles substrate hydrolyzed per min per mg protein) 10 49.51 ± 11.99. Expressions of tyrosine hydroxylase (TH) protein in the different organs from both head and body portions were comparatively analyzed using an immunohistochemical technique. Five organs of the medaka fish showing a strong TH protein expression were the olfactory bulb, hypothalamus, optic lobe, pons and myelencephalon regions. This study provides molecular and neurobehavioral bases of a biomonitoring system for toxic chemicals using fish as a model organism.

중학교 가정교과의 열린교육 활성화를 위한 수업 모듈 및 웹 기반 교육자료 개발

신상옥,유태명,한규정,윤지현,홍정숙,김유정,양정혜 한국 가정과 교육 학회 2000 한국가정과교육학회지 Vol.12 No.3

This study is about open and secondary level Home Economics Education which is applicable to field and which includes the characteristics, purpose and problems of each open education. secondary education and home economics education. Based on the ideology of open education, this study will identify the meaning of openness in terms of Home Economics Education reconstructing meaning. purpose, characteristics, curriculum paradigm and curriculum. The instructional model and module which use web, will be Presented as the results of the reconstruction works. The open instructional module in Home Economics Education, which will be operated on the web, is consisted of 1)setting the orientation of curriculum development, 2)instructional model development. 3)rebuilding the Home Economics Education curriculum 4)development of instructional modules and web-based materials and 5)construction of the web-site . Six instructional module begin with critical/emancipatory curriculum Paradigm in open Home Economics Education. use module as a unit of class management instead of 1 class. facilitates nest-type integration which is applied from open education keeping the nature of Home Economics Education. and focus on practical problem and problem solving through reconceptualization with dialectical perspective on individual, family and society. The practical problem solving model has four steps : Identifying practical problems. Understanding the contexts of Problem, Searching for solutions and examining its consequences . Action and evaluation . The web-site has to be developed based on the six instructional module includes those issues : self-identification for reinforcing autonomy and self-esteem. living with famity. living with friends, autonomous living. healthy living, and understanding sexuality.

구리 독성에 기인하는 비정상적인 어류행동의 신경생화학적 분석

신성우,조현덕,전태수,김정상,이성규,고성철 한국환경독성학회 2003 환경독성보건학회지 Vol.18 No.2

The goal of this study is to develop a biomarker used in monitoring abnormal behaviors of Japanese medaka (Oryzias latipes) as a model organism caused by hazardous chemicals. Japanese medaka was treated by copper of appropriate sublethal concentrations after starvation for 48 hr, The untreated individuals showed common behavioral characteristics (i.e., smooth and linear movements). Locomotive activity of the fish was monitored using an image processing and automatic data acquisition system. When treated with copper (100 ppb), the fish showed shaking patterns more frequently. As the concentration of copper increased to 1,000ppb, activity decreased, and the fish showed an erratic movement. Fish were exposed to copper at various concentrations (0, 100 and 1,000 pph) for 24 hrs, and acetylcholine esterase (AChE) activity was observed. When fish were exposed to 1,000 ppb of copper, the body AChE activities appeared to decrease but the head AChE activities showed little change. Expressions of tyrosine hydroxylase (TH) protein in the different organs from both head (brain) and body(kidney) portions affected by the copper treatment were analyzed using immunohistochemical technique compared with control. Five organs of the fish (olfactory bulb, hyothaiamus, optic lobe, pons and myelencephalon regions) showed a relatively strong TH protein expression in the control experiment. A differential expression of TH, however, was observed in the treatment (100 ppb and 1,0M ppb). The treatment (1,000 ppb) significantly suppressed TH protein production in the brain regions. In kidney, however, the same treatment caused little suppression compared with the control. Copper appeared to be less effective in suppression of TH than diazinon, a known TH suppressor. It was concluded that TH could be used as a potential biomarker to monitor the acute copper toxicity in Japanese medaka.

골격성 전치부 반대교합 아동의 치아성숙도에 관한 연구

신종현,권민석,김신,정태성 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.3

전치부 교환기의 반대교합 아동에서는 골격적 특성이 강할수록 상하 치아의 성숙도에 현격한 차이가 있음을 쉽게 관찰할 수 있다. 파노라마 방사선 사진으로 평가가 가능한 상하 치령의 차이가 III급 부정교합의 조기 징후로 개연성이 있다면, 이것은 조기 감별진단의 한 도구로써 가치가 있을 것으로 판단되었다. 부산대학교치과병원 소아치과에 내원한 Hellman 치령 IIA, IIC의 환자 를 대상으로, 측두방사선사진, 파노라마 방사선사진, 석고 모형과 임상사진을 조사하여 정상교합군과 전치부 반대교합군 각 50명을 선택하였다. 이들의 파노라마 방사선사진을 이용하여 Demirjian법으로 각 군의 상하 치령 및 제1대구치 맹출율을 구하고 그 차이를 비교, 분석하여 다음과 같은 결과를 얻었다. 1. 정상교합군과 반대교합군 모두에서 역령에 비해 치령이 높게 나타났으며 성차는 보이지 않았다(p>0.05). 2. 정상교합군과 반대교합군의 상하 치령의 차이는 각각 0.22세와 0.69세로 반대교합군에서 더 큰 차이를 보였다(p<0.05). 3. 정상교합군에 비해 반대교합군에서 상하 제1대구치 맹출율의 차이가 크게 나타났다(p<0.05). It was easy to find that children of a skeletal anterior crossbite in the early mixed dentition period showed a stark difference in the dental maturity between their maxillary and mandibular teeth, if they have stronger physical characteristics. If the difference of dental age between maxillary and mandibular teeth which can be identified via panoramic radiographs may serve as an early sign of class III malocclusion, this is considered valuable as a tool of early detection diagnosis. We obtained lateral cephalometric radiographs, panoramic radiographs, working model and clinical images of patients of Hellman dental age IIA and IIC who visited the department of pediatric dentistry, Pusan National University Dental Hospital and examined them to select 50 patents for normal occlusion group and skeletal anterior crossbite group, respectively. Their panoramic radiographs were used for the Demirjian's method to figure out dental ages of maxillary and mandibular teeth of each group and the eruption rate of the first molars. Their differences are as follows: 1. In both groups, the dental ages from Demirjian's method were advanced than the chronological ages. No sexual dimorphism was detected for the chronological or dental age in either group (p>0.05). 2. The difference of dental age of maxillary and mandibular teeth between the normal occlusion group and crossbite group was 0.22 and 0.69 years, respectively, with a higher difference in crossbite group(p<0.05). 3. Compared to the normal occlusion group, the crossbite group showed a higher difference in the eruption rate between maxillary and mandibular first molar(p<0.05).

초등학생의 Molar Incisor Hypomineralization 유병률과 제1대구치 치아우식증 및 수복상태 조사

신종현,안울진,김신,정태성 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.2

Molar Incisor Hypomineralization(MIH) have recently gained intensive research interests and various clinical trials. Most prevalence, etiology, treatment studies in MIH were carried out in the European countries, and data from the Korean were seldom. This study aimed to investigate the prevalence of MIH and the status of dental caries and treatment on the first permanent molars in primary school children. For this study, 1.344 primary school students in Busan and Ulsan cities were examined directly the permanent incisors and first molars. The results of the survey were as follows: 81 MIH cases were identified among 1.344 cases. The prevalence of MIH in this group of children was 6.0%. Children with MIH showed a significantly higher DMFT value for permanent teeth than children without MIH. The mean number of decayed, missed, and filled in 1st permanent molars(DMFT index) was 1.17. The rate of children with restoration on 1st permanent molar was 13.6% and the sequence of restoration materials was as follows: composite resin, amalgam, gold inlay. Molar Incisor Hypomineralization(MIH)에 관하여 최근 많은 연구적 관심이 집중되고 여러 임상적 대처방법들이 제시되고 있다. 이미 세계적으로는 MIH의 빈도, 병인, 치료 등에 관한 많은 연구보고가 있었으나, 우리나라에서는 이에 대한 관심이 아직 미미한 것으로 사료된다. 본 연구는 초등학생의 MIH 유병률과 제1대구치 우식 및 치료 실태에 대하여 조사할 목적으로 시도되었다. 부산 및 울산광역시에 거주하는 1,344명의 초등학생을 대상으로, 상하 제1대구치와 전치부를 직접 시진하여, MIH 유병률, 제1대구치 우식경험지수와 치료 상태를 조사하여 다음과 같은 결과를 얻었다. 총 1,344명 의 조사 대상 중 81명에서 MIH가 나타나, 유병률은 6.0%를 보였으며, MIH에 이환왼 어린이의 제1대구치 우식경험지수는 비이환군에 비해 통계적으로 유의하게 높았다(P<0.05) 한편, 제1대구치 우식경험지수는 1.17개로 나타났다. 조사 대상 제1대구치의 13.6%가 수복되어 있었고, 수복재 별로는 복합 레진, 아말감, gold inlay의 순이었다.

고실 사구종 1례

신현수,윤태현,장희종,남정권 대한이비인후과학회 1998 대한이비인후과학회지 두경부외과학 Vol.41 No.3