결핵성 흉막염 환자의 진단에 있어서 ICT Tuberculosis^(�) 검사를 이용한 유용성 연구

박희선,조은경,송창화,정성수,김주옥,김선영 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

The incidence of tuberculous pleuritis is high in Korea where the disease is endemic. Diagnosis of tuberculous pleuritis is often problematic for its difficulty in detecting microorganism by stain or culture and sometimes it is not available and to obtain specimens from the pleura because highly invasive procedures are needed. Repeated pleural biopsy is almost always hard for patients. Recent studies on tuberculosis have interest on new methods for accurate and rapid diagnosis. Serologic assay is one of the new imerging diagnostic method for tuerculosis and extrapulmonary tuberculosis. Total 38 subjects admitted in the Department of Respiratory Division of Internal Medicine of Chung-Nam National University Hospital are involved in this study. Median age of patients with tuberculous pleuritis was 40 years old, ranging from 18 to 88 years. All patients were receving treatmented by combination anti-tuberculous therapy after the test. The cardboard folding device contained a nitrocellulose strip on which the 38-kDa antigen from M. tuberculosis has been applied. When a specimen (pleural effusion, serum) flows past the nitrocellulose strip, antibodies specific for the 38-kDa antigen bind to the antigen line. In a reverse flow, the bound antibodies are detected by a goat anti-human immunoglobulin G antibody conjugated to colloidal gold particles and show a pink to red positive line. The test is completed in a maximum of 15 minites. Two of the 38 subjects showed positivity. One case was from the pleura and the other from the serum. From the above data, we concluded that ICT Tuberculosis^( ) test is not effective in the diagnosis of tuberculous pleuritis.

결핵균 30 kDa 항원과 Triton X-100 Solubilized Protein 항원에 의한 대장암 주변 림프절 단핵구의 활성화

박정규,김광호,조은경,임재현,민들레,송영자,김화중,백태현 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Tumor-draining lymph node mononuclear (TDLMN) cells are specifically sensitized to the growing tumor but such cells are deficient for mediating an antitumor response. In this study, we examined the feasibility of using mycobacterial 30 kDa or Triton X-100 solubilized protein (TSP) antigen to stimulate mononuclear cells of colon cancer-draining lymph node for the generation of cell mediated immune effector cells. The proliferative response of TDLMN cells stimulated with mycobacterial 30 kDa or TSP antigen was determined by ^(3)H-thymidine incorporation assay. The proliferation of TDLMN cells to mycobacterial 30 kDa or TSP antigen was significantly increased in PPD (+) patients, but a poor response to the 30 kDa or TSP antigen was observed in PPD (-). The expression on γδ T cells to mycobacterial 30 kDa or TSP antigen was assessed by flow cytometry. The γδ T cells from PPD ( + ) patient responded only to 30 kDa antigen but to TSP antigen. An investigation of cytokine mRNA expression was undertaken using reverse transcription-polymerase chain reaction (RT-PCR) to follow TDLMN cells stimulated with the 30 kDa or TSP antigens for 5 days. The IFN-γ and TNF-α mRNA expression was only induced in TDLMN cells of PPD ( + ) patient in response to the 30 kDa or TSP antigen. The IL-2 mRNA expression was induced in both PPD (+) and PPD (-) in response to the 30 kDa or TSP antigen. But the IL-4 mRNA expression was not induced in response to the 30 kDa or TSP antigen. These results suggest that the 30 kDa and TSP antigens may serve as biologic response modifier for the generation of cell mediated immune effector cells.

결핵군 55 kDa 항원의 면역학적 분석

김화중,김홍성,임재현,조은경,송창화,박정규 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1

Identification and characterization of mycobacterial antigens are critical for evaluation of their role in diagnosis, vaccination, and pathogenesis of mycobacterial diseases. An attempt has been made to immunologic characterize the 55 kDa protein antigen of Mycobacterium tuberculosis H37Rv which attracted our interest because it is present in high concentration in 50-80% ammonium sulfate fraction of the culture filtrate. Two monoclonal antibodies (MAbs) directed to 55 kDa antigen were produced. MAbs MT55-1 and MT55-2 reacted with a single 55 kDa protein band. On examination of degree for cross-reactivity with other mycobacterial species by enzymelinked immunosorbent assay (ELISA) and immunoblotting, these antibodies reacted strongly with M. tuberculosis and M. bovis BCG, and reacted weakly with M. marinum and M. smegmatis. To investigated the subcellular distribution of MAbs defined epitopes in the 55 kDa antigen within the mycobacterium, we isolated three major subcellular factions of M. tuberculosis, namely, cell wall, cytoplasmic membrane, and cytosol, by a simple fractionation procedure. MAb MT55-1 reactive cpitope was found in the cytosol when tested by immunoblotting. A sandwich ELISA was initially developed for detecting 55 kDa antigen using MT55-1 MAb in mycobacterial culture filtrate before detecting it in clincal specimens. The minimal detectable concentration was 1.0 ㎍/m1 for M. tuberculosis culture filtrate and 100 ㎍/ml for sonic extracts of M. bovis BCG and M. marinum, respectively. But the 55 kDa antigen was not detected in sonic extracts of other mycobacterial species examined. Although further evaluations are required, this study suggests that the 55 kDa antigen may be of interest as potential diagnostic reagent.

HL-60 세포주를 이용한 결핵균항원의 세포성면역반응의 분석

박정규,강윤중,김운옥,임재현,송창화,조은경,김화중 충남대학교 의학연구소 2001 충남의대잡지 Vol.28 No.2

Most persons who become infected with M. tuberculosis mount a protective immune response and remain clinically well, the only evidence of infection being development of a positive tuberculin skin test. Five to 10% develop tuberculosis disease within the first 2 years after infection (primary tuberculosis) or thereafter (reactivation tuberculosis). Acquired resistance against tuberculosis paradigmatically rests on cell-mediated immunity, with the major factors being mononuclear phagocytes and T Lymphocytes. While the former cells act as the principal effectors, the latter ones serve as the predominant inducers of protection. The usefulness of the single dose of BCG routinely given in childhood in many developing countries in preventing far more commonly occurring tuberculosis in adults is in doubt. An effective and safe vaccine against tuberculosis is sorely needed. A subunit vaccine are capable of inducing protective immunity and could have substantial advantages over BCG or other whole-bacterium vaccines. The human promyelocytic cell line HL-60 adopted characteristic macrophage-like properties, including adherence and CD14 expression after a period of continuous culture at high ambient CO_(2) concentration. When HL-60 cells were cultured with 1,25-dihydroxyvitamin D_(3) for 4 days, the cells acquired the activity to potentiate T cell proliferation by the 30 kDa or 38 kDa antigen of Mycobacterium tuberculosis H37Rv Therefore, vitamin D-treated HL-60 cells showed the function of the antigen presenting cells.

Distributed memory access architecture and control for fully disaggregated datacenter network

Kyeong-Eun Han(Kyeong-Eun Han),Ji Wook Youn(Ji Wook Youn),Jongtae Song(Jongtae Song),Dae-Ub Kim(Dae-Ub Kim),Joon Ki Lee(Joon Ki Lee) 한국전자통신연구원 2022 ETRI Journal Vol.44 No.6

In this paper, we propose novel disaggregated memory module (dMM) architecture and memory access control schemes to solve the collision and contention problems of memory disaggregation, reducing the average memory access time to less than 1 μs. In the schemes, the distributed scheduler in each dMM determines the order of memory read/write access based on delay-sensitive priority requests in the disaggregated memory access frame (dMAF). We used the memory-intensive first (MIF) algorithm and priority-based MIF (p-MIF) algorithm that prioritize delay-sensitive and/or memory-intensive (MI) traffic over CPU-intensive (CI) traffic. We evaluated the performance of the proposed schemes through simulation using OPNET and hardware implementation. Our results showed that when the offered load was below 0.7 and the payload of dMAF was 256 bytes, the average round trip time (RTT) was the lowest, ~0.676 μs. The dMM scheduling algorithms, MIF and p-MIF, achieved delay less than 1 μs for all MI traffic with less than 10% of transmission overhead.

Selection and Characterization of Transposon Tagging Mutants of Synechocystis sp. PCC 6803 Sensitive to High-Light and Oxidative Stresses

( Eun Kyeong Song ),( Ismayil S. Zulfugarov ),( Jin Hong Kim ),( Eun Ha Kim ),( Woo Sung Lee ),( Choon Hwan Lee ) 한국식물학회 2004 Journal of Plant Biology Vol.47 No.4

We compared several analytical tools to identify which were most applicable for the selection and characterization of specific transposon-tagged mutant strains of Synechocystis sp. PCC 6803 that are sensitive to high light and oxidative stresses. Our primary parameter was the maximum photochemical efficiency of dark-adapted cells, a very sensitive factor that can be determined in a non-destructive manner. Using this as a tool for primary selection, we identified five mutant strains with different sensitivities to photoinhibition and photooxidation. For further characterization, we obtained data describing the absorption spectra for pigment contents, the 77 K fluorescence spectra, non-photochemical quenching (as a down-regulation process), and the photosynthetic electron transfer rate. Based on these results, we were able to design a strategy for selecting mutants with specific phenotypes. Here, we also discuss the strengths and weaknesses of each selection and characterization tool.

Ferulate, an Active Component of Wheat Germ, Ameliorates Oxidative Stress-Induced PTK/PTP Imbalance and PP2A Inactivation

Eun Mi Koh,Eun Kyeong Lee,Chi Hun Song,Jeongah Song,Hae Young Chung,Chang Hoon Chae,Kyung Jin Jung 한국독성학회 2018 Toxicological Research Vol.34 No.4

Ferulate is a phenolic compound abundant in wheat germ and bran and has been investigated for its beneficial activities. The aim of the present study is to evaluate the efficacy of ferulate against the oxidative stress-induced imbalance of protein tyrosine kinases (PTKs), protein tyrosine phosphatases (PTPs), and serine/threonine protein phosphatase 2A (PP2A), in connection with our previous finding that oxidative stress-induced imbalance of PTKs and PTPs is linked with proinflammatory nuclear factor-kappa B (NF-κB) activation. To test the effects of ferulate on this process, we utilized two oxidative stress-induced inflammatory models. First, YPEN-1 cells were pretreated with ferulate for 1 hr prior to the administration of 2,2’-Azobis(2-methylpropionamidine) dihydrochloride (AAPH). Second, 20-month-old Sprague-Dawley rats were fed ferulate for 10 days. After ferulate treatment, the activities of PTKs, PTPs, and PP2A were measured because these proteins either directly or indirectly promote NF-κB activation. Our results revealed that in YPEN-1 cells, ferulate effectively suppressed AAPH-induced increases in reactive oxygen species (ROS) and NF-κB activity, as well as AAPH-induced PTK activation. Furthermore, ferulate also inhibited AAPH-induced PTP and PP2A inactivation. In the aged kidney model, ferulate suppressed aging-induced activation of PTKs and ameliorated aging-induced inactivation of PTPs and PP2A. Thus, herein we demonstrated that ferulate could modulate PTK/PTP balance against oxidative stress-induced inactivation of PTPs and PP2A, which is closely linked with NF-κB activation. Based on these results, the ability of ferulate to modulate oxidative stress-related inflammatory processes is established, which suggests that this compound could act as a novel therapeutic agent.

캡슐내시경과 단일풍선 소장내시경으로 진단된 공장의 간질종양 1예

송도경,심기남,태정현,김경진,송명은,송하응,윤혜원,정가영,정정화 이화여자대학교 의과학연구소 2012 EMJ (Ewha medical journal) Vol.35 No.2

Gastrointestinal stromal tumors (GISTs) are common mesenchymal tumors that arise in the wall of the gastrointestinal tract. We report a case of obscure gastrointestinal bleeding due to a GIST of the jejunum successfully documented by videocapsule endoscopy (VCE) and single-balloon enteroscopy (SBE). A 36-year-old man with hematochezia was referred for further evaluation of no evidence of bleeding focus on esophagogastroduodenoscopy and colonoscopy. A VCE showed a suspicious ulcerative hyperemic mass that located in about 1 hour apart from duodenal second portion. SBE revealed a nonbleeding 4×2 cm mass with an ulcer at the proximal jejunum. The patient underwent laparoscopic resection without complication. Histological examination revealed a well circumscribed, dumbbell-shaped firm mass comprised of spindle cells. Immunohistochemical staining for CD 117 was diffusely positive, whereas staining for S-100, CD 34 and MIB-1 was all negative. It was confirmed to be a low-grade GIST at the proximal jejunum.

복통을 호소한 20세 환자에서 발견된 인식되지 못한 질내 이물

오은경 ( Eun Kyeong Oh ),송재연 ( Jae Yeon Song ),조현희 ( Hyun Hee Jo ),권동진 ( Dong Jin Kwon ),임용택 ( Yong Taik Lim ),유영옥 ( Young Oak Lew ),김은중 ( Eun Jung Kim ),김장흡 ( Jang Heup Kim ),김미란 ( Mee Ran Kim ) 대한산부인과학회 2010 Obstetrics & Gynecology Science Vol.53 No.8

The foreign bodies in vagina cause intense inflammation. Genital complaints in patients could indicate the presence of a vaginal foreign object. Vaginal bleeding and blood-stained, foul-smelling discharge are considered to be the main clinical manifestations of vaginal foreign bodies, and toilet tissue reported as the most commonly found foreign body. The insertion of foreign bodies into the vagina is not uncommon but presentation as lower abdominal pain in an gynaecological clinic is rare. The causes of insertion are sexual stimulation, sexual abuse, accident of post-surgery and most cases find a solution after vaginal speculum examination. We describe a case of foreign body in the vagina of a patient presenting with chronic lower abdominal pain but undetectable and unrecognized in general examination.

충남지역 일부 시설노인과 재가노인의 식생활 및 영양섭취상태 비교

송은숙(Eun-Sook Song),김은진(Eun-Jin Kim),김명희(Myung-Hee Kim),최미경(Mi-Kyeong Choi) 동아시아식생활학회 2011 동아시아식생활학회지 Vol.21 No.5