RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

Antiadhesive effect and safety of oxidized regenerated cellulose after thyroidectomy

Kyoung Sik Park,Kyu Eun Lee,Do Hoon Ku,Su-Jin Kim,Won Seo Park2,Hoon Yub Kim3,,Mi Ra Kwon1,Yeo-Kyu Youn1 대한외과학회 2013 Annals of Surgical Treatment and Research(ASRT) Vol.84 No.6

Purpose: To evaluate the antiadhesive effects and safety of an oxidized regenerated cellulose (Interceed) after thyroidectomy. Methods: Seventy-six thyroidectomized patients were prospectively randomized into two groups with regard to the use of Interceed. We evaluated each group for their adhesive symptoms using four subjective and four objective items at the 2nd week, 3rd and 6th month after thyroidectomy. All patients were examined for vocal cord motility by indirect laryngoscope at each period. Results: Total adhesion scores at each postoperative follow-up period decreased with time, but were not significantly different in each group. The median score for swallowing discomfort for liquid was significantly lower in the Interceed group than in the control group 2 weeks after surgery. In addition, the severity of skin adhesion to the trachea was reduced in the Interceed group compared with the control group 6 months after surgery. During the study, there were no adverse effects or significant differences in postoperative complications between the groups. Conclusion: Interceed appeared to be safe and effective in improving neck discomfort at early postoperative periods and preventing skin adhesion to the trachea 6 months after thyroidectomy.

후두적출자의 의사소통 태도

강수균,박선희,백은아,이현혜,최경희,강은희,박은실 대구대학교 특수교육재활과학연구소 2005 특수교육재활과학연구 Vol.44 No.3

본 연구는 후두적출자의 주된 발성방법과 후두적출 경과 시기에 따른 의사소통 태도를 살펴보고자 하였다. 연구의 대상은 성인 남자 55명으로 후두암으로 인한 후두적출자들로 구성되었다. 이들에게 실시한 의사소통 태도 설문지는 Erickson의 의사소통 태도 척도-수정판(Modified Erickson Scale of Communication Attitudes: S-24)을 본 연구에 맞게 수정 보완하여 사용하였다. 본 연구의 결과는 첫째, 주된 발성방법에 따라서는 인공후두기 사용자와 식도발성자간의 의사소통 태도에 차이가 없었다. 둘째, 후두적출 경과 시기에 따라 10년 초과된 후두적출자들과 10년 이하의 후두 적출자들 간의 의사소통 태도에 차이가 없었다. 그러므로 이들의 의사소통 태도의 긍정적 태도를 증가시키기 위하여 다양한 방법을 활용하고 습득하여 이들의 의사소통 능력을 빠르게 재습득 하여 긍정적인 의사소통 능력을 가질 수 있도록 해야 한다. The purpose of this study was to determine laryngectomee's communication attitude. 55 laryngectomees, who were in the range of 40 years old to 80 years old participated in the study. Communication attitude measurements were taken by S-24(Modified Erickson Scale of Communication Attitudes). The results showed as follows: First, score of laryngectomees with artificial larynx and esophageal speakers were no significant. Second, score of laryngectomees with over 10 years after latyngectomy and 10 years after latyngectomy were no significant.

외국인 유학생의 국제이주와 지역사회 적응에 관한 연구: 대구·경북지역 대학을 중심으로

박은경 대구대학교 다문화사회정책연구소 2011 현대사회와 다문화 Vol.1 No.2

2007년 국내 고등교육기관에서 수학하고 있는 외국인 유학생의 수는 4만 9천여명을 넘어섰다. 이러한 분위기에서 외국인 유학생들에 관한 논의들이 몇 가지 측면에서 진행되고 있다. 그러나 대체로 유학생의 유치를 위한 전략 또는 유학생의 학업적 적응, 문화적 적응과 같은 단편적인 측면에서의 적응만을 조사한 연구들이 대부분이었다. 이러한 분위기에서 외국인 유학생들에 관한 논의들이 몇 가지 측면에서 진행되고 있다. 그러나 대체로 유학생의 유치를 위한 전략 또는 유학생의 학업적 적응, 문화적 적응과 같은 단편적인 측면에서의 적응만을 조사한 연구들이 대부분이었다. 이를 바탕으로 진행한 연구의 결과를 요약하면 다음과 같다. 첫째, 외국인 유학생의 국내 공간적 분포 특성은 유학생 유입 초기단계에는 수도권에 압도적으로 많이 이루였으나, 그 후 수도권의 집중현상은 점차 완화되고 있다. 둘째, 설문조사 에서는 외국인 유학생의 국제이주 원인으로 거시적 차원의 배출-흡인 요소 보다는 미시적 차원의 배출-흡인요소가 더 많은 영향을 주는 것으로 나타났다. 셋째, 외국인 유학생의 지역사회 적응에 필요한 각 요소별 적응은 개인별로 상이하게 나타난 다. 특히 한국어 구사능력이 높고 한국인 친구의 수가 많을 수록 대학 및 지역사회에서 적응하기가 용이한 것으로 조사되었다. 또한 유학생활과 지역사회에서의 적응정도가 높을수록 지역사회에서 느끼는 정체성도 높게 나타났다. 이를 통해 지역사 회 주민으로서 정체성이 높게 형성된 외국인 유학생에게 지역사회의 발전에 기여하는 역할을 기대해 볼 수 있다. The number of foreign students studying in domestic higher educational institutes in South Korea exceeded 49,000 in 2007. One reason to pay attention to the rapid expansion of foreign students is that the impact of them on local community also becomes greater as their proportion increases. Under this circumstance, considerable discussions concerning them from several aspects have been undertaken. However, most of researches are limited to partial aspects of either strategy to attract foreign students or their academic and cultural adaptation. Therefore, this study selects universities in Daegu and Gyeongbuk area and intends to analyze the migration causes, to figure out the characteristics of their spatial distribution, to examine their adaptational patterns and finally to suggest some measures for them. The study results based on these are summarized as follows: Firstly, as for foreign students’ spatial distribution, Seoul and its surrounding area was where foreign students are most frequently located at the early stage of their migration. Secondly, the questionnaire survey results show that the macro level of push-out/pull-in factors has a greater impact on foreign students’ migration than the micro level of them. Thirdly, the adaptation patterns across each dimension show individual differences. In particular, the foreign students who have a higher Korean language ability and more Korean friends show a better adaptation to university and local community. In addition, the results show that those with higher adaptation level to the life of study abroad and local community have a higher level of perceived identity as a member of their local community, through which a contribution of those foreign students with higher identity as a community member to the development of the local community may be anticipated.

노인을 위한 건강관련 프로그램의 효과

박인기,민경은 忠南大學校體育科學硏究所 1999 體育科學硏究誌 Vol.17 No.1

The Purpose of this study was to determine effects of program for improving health related physical fitness developed for older people by measuring the changes in the participant's muscle strength, muscle endurance, flexiability, cardiovascular fitness. This study employed 36 healthy older people who were 60∼65 years old (mean age 62.5years). The subjects were randomly assigned to one of three gruops (two experimental groups and one control). Each group ended up with 12 subjects. The results of the study were as follows : 1. The program for improving health related physical fitness program administered to the experimental groups showed statistically significant effects with respect to the positive changes in cardiovascular fitness during the treatment period. 2. The physical fitness program administered to the experimental groups showed statistically significant effects with respect to the positive changes in flexibility. 3. The program for improving health related physical fitness program administered to the experimental groups showed statistically significant effects with respect to the positive changes in musclular endurance during the treatment period. 4. The program for improving health related physical fitness program administered to the experimental groups showed statistically significant effects with respect to the positive changes in musclular strength during the treatment period.

에어로빅운동시 심박수를 이용한 소비에너지량 산출에 관한 연구

박철호,박은경 東亞大學校附設스포츠科學硏究所 1998 스포츠科學硏究論文集 Vol.16 No.-

This study aims to investigate an exercise and to calculate the energy expenditure by the old women in their sixties who take part in the aerobic exercise program of L-Cultural Center in Pusan by using the formula related to their Heart rate and oxygen intake. The conclusion can be obtained from this study as follows: 1. when they do the aerobic exercise, their Heart rate is at 116.8±28.46 beats/min 2. when they do the aerobic exercise, their exercise intensity is at 57.93±21.31%HRmax. 3. when they do the aerobic exercise, their quantity of energy consumpton is at 3.92 Kcal/min, 5758.8 Kcal/day, 3.83MET. 4. there is a high correlation among their Heart rate, respiratory rate, ventilation and other physiological variant factors. Therefore, 50~70%HRmax will be needed in order to improve their sustaining power of whole body. In this respect, judging from their average exercise intensity, they take part in the program of the aerobic exercise in order to improve their ability of an aerobic exercise.

백서의 치아이동 시 피질골 천공이 치주조직의 OPG, RANK, RANKL의 발현에 미치는 영향

박우경,김성식,박수병,손우성,김용덕,전은숙,박미화 대한치과교정학회 2008 대한치과교정학회지 Vol.38 No.3

치아이동 시 피질골 천공이 치조골 재형성에 미치는 영향을 알아보기 위해서 생후 15주된 자성백서를 사용하여 피질골 천공 후 치아이동을 실시한 실험군(Tooth movement with cortical punching: TMC group, n = 16)과 교정적 치아이동만 실시한 대조군(Tooth movement only group: TM group, n = 16)의 치아주위조직을 면역조직화학염색을 통하여 관찰하였다. 실험군과 대조군의 실험동물에 20 gm의 힘으로 상악 전치부 사이를 이개시키는 치아이동을 시행하였으며 실험군에서는 상악 전치부 구개부위에 피질골 천공을 실시하였다. 치아이동 후 1, 4, 7, 14일째에 실험군과 대조군의 실험동물을 희생시켰다. 면역조직화학염색법으로 OPG, RANK, RANKL의 발현을 비교한 결과, OPG의 발현은 양 군 모두에서 미처치 대조군에 비하여 감소되었으나, 실험군에서의 발현이 대조군보다 컸으며, RANK, RANKL은 피질골 천공을 시행한 경우에 더 강한 발현을 보이는 것이 관찰되었다. 따라서 피질골 천공이 치주조직의 OPG, RANK, RANKL의 발현에 영향을 미치며 치조골의 재형성을 향상시키는 것을 알 수 있었다. Objective: The purpose of this study was to investigate whether cortical punching could stimulate the expression of OPG, RANK, and RANKL during tooth movement by immunohistochemistry. Methods: 34 sprague-dawley rats (15 weeks old) were allocated into 3 groups: TMC group (experimental group; Tooth Movement with Corticotomy, n = 16), TM group (control group; Tooth Movement only group, n = 16), and non-treatment group (n = 2). 20 gm of orthodontic force was applied to rat incisors by inserting elastic bands. The duration of force application was 1, 4, 7 and 14 days. A microscrew (diameter 1.2 mm) was used for cortical punching of the palatal side of the upper incisors in the TMC group. Results: Distributions of OPG, RANK, and RANKL were evaluated by immunohistochemistry. OPG, RANK and RANKL were observed on experimental and control groups. On the compression side, the degree of the expression of OPG decreased in both groups. The expression of RANK was most prominent in the experimental group of day 4. The expression of RANKL was most intensive and extensive in the experimental group of day 7. However, the expression of OPG was decreased in the experimental and control groups compared to the non treatment group. The expression of OPG, RANK and RANKL after force application were decreased at day 14. Conclusions: These findings suggested that cortical punching might stimulate remodeling of alveolar bone during a 2 week period of tooth movement without any pathologic change.

비만학생들의 운동후 회복시 초과 산소소비량 및 지속시간에 관한 연구

박철호,권민호,고봉민,박은경,우상헌 東亞大學校附設스포츠科學硏究所 1999 스포츠科學硏究論文集 Vol.17 No.-

The purpose of this study was to examine the effect of two different intensity of continuous exercise on magnitude of excess post-exercise oxygen consumption(EPOC), energy consumption following a 20min treadmill exercise. The subjects of this study were eight super-obesity in middle school students who were above 13 years old. subjects participated in two different exercise intensity to measure respiratory gas, blood lactate concentration and eardrum temperature. The results of this study were as follows: 1. The Energy consumption of the high intensity (80% VO₂ max) exercise group (264.68 35.60cal) was greater than that of the low intensity (50%) exercise group(172.01 20.39 Cal)(p<.001). 2. The EPOC of the high intensity exercise group (22.36+ 6.82Cal ) was greater than that of the low intensity exercise group(15.39+5.27 Cal)(p<.001). 3. The EPOC duration of th high intensity exercise group(28.36=3.56min) was longer than that of the low intensity exercise group(17.23+1.25min)(p<.001) 4. The blood lactate concentration, respiratory exchange ratio and temperature of the high intensity exercise group were greater than those of the low intensity exercise group(p<.05,p<.01). EPOC and duration were increase with 80%VO₂max, 80% VO₂max as intensity exercise got higher this also showed that it had something to do with lactate concentration in blood and increase of body temperature in the conclusion. We considered that there was a difference in using methods of energy, as there was a similar difference in RER. We though this following with oxygen consumption after exercise, we could clarify that high intensity exercise was better than lower intensity exercise in sametime for those who are obese especially adolescents to maintain their weights on the aspect of effect.

무산소 운동 후 발마사지가 피로회복에 미치는 영향

박인기,민경은 忠南大學校體育科學硏究所 2001 體育科學硏究誌 Vol.19 No.1

The study was aimed to identify the effects of foot massage on fatigue recovery after anaerobic exercise. 10 subjects on basketball player performed anaerobic exercise(wingate test)to examine the physical work capacity. Each subject was treated one of two recovery methods(foot massage, rest recovery). Blood lactate was analyzed at pre-exercise, 5, 10 and 15 minutes. The major finds of this study were as follows; first, Blood lactate concentration was removed more rapidly with foot massage than with rest recovery during the post-exercise recovery period. second, Recovery time of blood lactate concentration was significant from 10 to 15minutes with foot massage during the post-exercise recovery period. In conclusion, The result of this study was noticed that fatigue is recovered more rapidly with foot massage than with rest recovery method after maximal exercise. It means that the foot massage is considered as a means of keeping the physical activity and sports.