서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

Enhanced Whole-cell Biodehydrogenation of 11β-hydroxyl Medroxyprogesterone in a Biphasic System Containing Ionic Liquid

Jun-Yao He,Pu Wang,Yu-Fen Yang,Song-Lin Xie 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.5

A hydrophilic ionic liquid, 1-butyl-3-methylimidazolium (L)-lactate ([bmim][lactate]), was successfully employed as a co-solvent to improve the biodehydrogenation of steroid 11β-hydroxyl medroxyprogesterone (HMP)to pregna-1,4-diene-6α-methyl-3,20-dione-11β,17α-dihydroxy (PDMDD) in an aqueous-organic biphasic system using Arthrobacter simplex UR106 cells. First, a suitable biphasic system was constituted, composed of toluene and Tris-HCl buffer (0.05 mol/L, pH 7.6) at a 0.4 (V_(org)/V_(aq))volumetric phase ratio. Investigation of biodehydrogenation with the addition of [bmim][lactate] to the aqueous phase was then performed. The results demonstrate that biodehydrogenation is more efficient in a biphasic system containing 2% [bmim][lactate] as a co-solvent compared to an aqueous-toluene two-phase system. Higher bioconversion (83.2% versus 56.4%) was observed after 16 h at a substrate concentration of 80 mg HMP in 8 mL of toluene.

Mechanical Behavior and Analysis of Composite Bridges with Corrugated Steel Webs: State-of-the-Art

Jun He,Yuqing Liu,Airong Chen,Teruhiko Yoda 한국강구조학회 2012 International Journal of Steel Structures Vol.12 No.3

The composite bridges with corrugated steel webs have excellent properties, such as lightness of girders, efficiency of introducing prestress forces, short construction period, optimum force distribution, good seismic performance, and aesthetics appearance etc., which have greatly promoted the application of such bridges. The objective of this paper is to provide and summarize important references related to the analysis, design and construction of composite bridges with corrugated steel webs. Subjects discussed in this review include (1) structural configuration and application; (2) shear behavior; (3) bending behavior; (4) torsional behavior; (5) patch loading resistance; (6) dynamic behavior; (7) long-term behaviors including fatigue and creep; (8) component connections; (9) analysis method and theory; (10) new concept application. The literature survey presented herein mainly focuses on papers written in English, Japanese, German and Chinese in relation to composite bridges with corrugated steel webs.

Effect of Ginger Extract and Citric Acid on the Tenderness of Duck Breast Muscles

He, Fu-Yi,Kim, Hyun-Wook,Hwang, Ko-Eun,Song, Dong-Heon,Kim, Yong-Jae,Ham, Youn-Kyung,Kim, Si-Young,Yeo, In-Jun,Jung, Tae-Jun,Kim, Cheon-Jei Korean Society for Food Science of Animal Resource 2015 한국축산식품학회지 Vol.35 No.6

The objective of this study was to examine the effect of ginger extract (GE) combined with citric acid on the tenderness of duck breast muscles. Total six marinades were prepared with the combination of citric acid (0 and 0.3 M citric acid) and GE (0, 15, and 30%). Each marinade was sprayed on the surface of duck breasts (15 mL/100 g), and the samples were marinated for 72 h at 4℃. The pH and proteolytic activity of marinades were determined. After 72 h of marination, Warner Bratzler shear force (WBSF), myofibrillar fragmentation index (MFI), pH, cooking loss, moisture content, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and protein solubility were evaluated. There was no significant (p>0.05) difference in moisture content or cooking loss among all samples. However, GE marination resulted in a significant (p<0.05) decrease in WBSF but a significant (p<0.05) increase in pH and MFI. In addition, total protein and myofibrillar protein solubility of GE-marinated duck breast muscles in both WOC (without citric acid) and WC (with citric acid) conditions were significantly (p<0.05) increased compared to non-GE-marinated duck breast muscles. SDS-PAGE showed an increase of protein degradation (MHC and actin) in WC condition compared to WOC condition. There was a marked actin reduction in GE-treated samples in WC. The tenderization effect of GE combined with citric acid may be attributed to various mechanisms such as increased MFI and myofibrillar protein solubility.

Loading capacity evaluation of composite box girder with corrugated webs and steel tube slab

Jun He,Yuqing Liu,Xiaoqing Xu,Laibin Li 국제구조공학회 2014 Structural Engineering and Mechanics, An Int'l Jou Vol.50 No.4

This paper presents a type of composite box girder with corrugated webs and concrete filled steel tube slab to overcome cracking on the web and reduce self-weight. Utilizing corrugated steel web improves the efficiency of prestressing introduced into the top and bottom slabs due to the accordion effect. In order to understand the loading capacity of such new composite structure, experimental and numerical analyses were conducted. A full-scale model was loaded monotonically to investigate the deflection, straindistribution, loading capacity and stiffness during the whole process. The experimental results show that test specimen has enough loading capacity and ductility. Based on experimental works, a finite element (FE) model was established. The load-displacement curves and stress distribution predicted by FE model agreewell with that obtained from experiments, which demonstrates the accuracy of proposed FE model. Moreover, simplified theoretical analysis was conducted depending on the assumptions which were confirmed by the experimental and numerical results. The simplified analysis results are identical with the tested and numerical results, which indicate that simplified analytical model can be used to predict the loading capacity of such composite girder accurately. All the findings of present study may provide reference for the application of such structure in bridge construction.

Experimental and Numerical Study on Cyclic Behaviour of Steel Beam-to-Column Joints

Jun He,Teruhiko Yoda,Hideaki Takaku,Yuqing Liu,Airong Chen,Masashi Iura 한국강구조학회 2010 International Journal of Steel Structures Vol.10 No.2

Ten specimens are tested to investigate the cyclic behavior of beam-to-column joints of steel frames with joint panels. The performances of the joints with respect to strength, rigidity, and hysteretic performance are examined. Three different loadcarrying mechanisms can be identified. Panel resistance ratio (R p) is presented for predicting the buckling patterns. The validity of R p is confirmed through the present experimental results. On the basis of the experimental results of steel beam-to-column moment joints, 3-D nonlinear finite element models are established to analyze the mechanical properties of these connections. The load-displacement curves of the finite element analysis are in good agreement with those of the tests in terms of strength and unloading stiffness. A shear lag phenomenon was captured in the beam flanges by not only experimental results but also numerical analysis. Parametric studies are conducted on the connections under monotonic loading to investigate the influences of connection dimension, resistance ratio on the connection behavior. It was found that the failure modes are influenced by the resistance ratio, while the thickness of joint panels resulting in large effects on the strength and stiffness under shear failure mode.

Neuron Discharge and c-Fos Expression in the Nucleus of the Solitary Tract Following Electroacupuncture at Acupoints of the Yangming Stomach Meridian of Foot

Jun-Feng He,Jie Yan,Jiang-Shan Li,Jian-Hua Liu,Chao Wang,Xiao-Rong Chang,Ya-Ting Qu 사단법인약침학회 2013 Journal of Acupuncture & Meridian Studies Vol.6 No.2

The nucleus of the solitary tract (nucleus tractus solitarii; NTS) is a primary center for both visceral afferents and somatic afferents. Previous experiments have demonstrated that the NTS is closely connected to the stomach and acupoints in the Yangming Stomach Meridian of Foot (ST Meridian). In this study, extracellular recording and immunochemistry methods were used to analyze the discharge of neurons and c-Fos protein expression in the NTS following acupuncture at different acupoints and a nonacupoint. A total of 104 discharging neurons were detected in the NTS of 52 rats, of which 86 provided complete data. After acupuncture at Sibai (ST 2), Zusanli (ST 36), Neiting (ST 44), Quanliao (SI 18), and the nonacupoint, the neuron response rate in the NTS was 65.12%, 51.16%, 46.51%, 34.88% and 31.40% respectively. For neuron response rate, there was a significant difference among Sibai (ST 2), Zusanli (ST 36), Neiting (ST 44), Quanliao (SI 18), and the nonacupoint (p < 0.01 or p < 0.05). In the other 48 rats, the number of c-Fos immunoreactive neurons in the NTS by electroacupuncture (EA) at Sibai (ST 2) group was significantly higher than that EA at other acupoints and the nonacupoint (p < 0.05 or p < 0.01). EA at both Zusanli (ST 36) and Neiting (ST 44) increased c-Fos immunoreactive neurons significantly over EA at Quanliao (SI 18) and the nonacupoint (p < 0.05 or p < 0.01), while there was no difference between EA at Quanliao (SI 18) and the nonacupoint group (p > 0.05). The experiments demonstrated that the afferent convergence in NTS are different by body surface points stimulus, which suggests that the NTS might be a primary center in the central nervous system receiving acupoints stimulus from the ST Meridian.

Effect of Curing Temperature on the Geotechnical Behavior of Solidified Sludge in Landfill Temporary Cover Applications

Jun He,Li-Rong Zhou,Lei Zhang,Xiao-Ying Feng 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.4

Elevated temperature in municipal solid waste will affect the performance of earthen materials in a landfill cover liner system. Soda residue (SR), ground granulated blast furnace slag, and quicklime were utilized as solidifiers in sewage sludge, and the curing temperature effect on geotechnical behavior of solidified sludge in landfill temporary cove applications was investigated. The unconfined compressive strength, yield stress, coefficient of compressibility, hydraulic conductivity, and microstructural and mineralogical characteristics of solidified sludge at various curing temperatures were measured. Hydration products including ettringite crystals and calcium-silicate-hydrates (C-S-H) were observed in the samples. An increase in temperature from 12 to 40°C improved the solidified sludge strength, but a further increase to 60°C decreased the strength. The lowest coefficient of compressibility and the lowest hydraulic conductivity were observed at 40°C. The pore volume or pore size of the solidified sludge decreased with the increase in temperature. However, the geotechnical performance was improved due to the increase in C-S-H and decrease in ettringite in 40°C sample. 50% SR content was optimum for solidified sludge temporary cover due to its relatively high strength, high yield stress, low compressibility, moderate hydraulic conductivity, and good pollution stabilization capability at different curing temperatures.

Facial Action Unit Detection with Multilayer Fused Multi-Task and Multi-Label Deep Learning Network

( Jun He ),( Dongliang Li ),( Sun Bo ),( Lejun Yu ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.11

Facial action units (AUs) have recently drawn increased attention because they can be used to recognize facial expressions. A variety of methods have been designed for frontal-view AU detection, but few have been able to handle multi-view face images. In this paper we propose a method for multi-view facial AU detection using a fused multilayer, multi-task, and multi-label deep learning network. The network can complete two tasks: AU detection and facial view detection. AU detection is a multi-label problem and facial view detection is a single-label problem. A residual network and multilayer fusion are applied to obtain more representative features. Our method is effective and performs well. The F1 score on FERA 2017 is 13.1% higher than the baseline. The facial view recognition accuracy is 0.991. This shows that our multi-task, multi-label model could achieve good performance on the two tasks.

Prognostic Significance of Overexpression of EZH2 and H3k27me3 Proteins in Gastric Cancer

He, Long-Jun,Cai, Mu-Yan,Xu, Guo-Liang,Li, Jian-Jun,Weng, Zi-Jin,Xu, Da-Zhi,Luo, Guang-Yu,Zhu, Sen-Lin,Xie, Dan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

The enhancer of zeste homolog 2 (EZH2) methyl transferase and histone 3 lysine 27 (H3K27me3) protein can repress gene transcription, and their aberrant expression has been observed in various human cancers. This study determined their expression levels in gastric cancer tissues with reference to clinicopathological features and patient survival. We collected 117 gastric cancer and corresponding normal tissues for immunohistochemistry analysis. In gastric cancers, 82/117 (70.1%) were positive for EZH2 and 66/117 (56.4%) for H3K27me3 proteins in contrast to only 5.41% and 7.25% of normal gastric mucosa specimens, respectively. Kaplan-Meier survival data showed the average overall and disease-free survival of EZH2 high expression patients was 25.2 and 20.2 months, respectively, shorter than that with EZH2 low expression (40.5 and 35.9 months). The average overall survival and disease-free survival of high H3K27me3 expression patients was 23.4 and 17.4 months, shorter than without H3K27me3 expression (37.6 and 34.5 months). The average overall survival and disease-free survival of patients with both EZH2 and H3K27me3 expression was 18.8 and 12.9 months, respectively, shorter than that with either alone (34.7 and 31.2 months) or with low levels of both (43.9 and 39.9 months). Multivariate Cox regression analysis showed that H3K27me3 and EZH2 expression, tumor size differentiation and clinical stage were all independent prognostic factors for predicting patient survival. This study demonstrated that detection of both EZH2 and H3K27me3 proteins can predict poor survival of gastric cancer patients, superior to single protein detection. In addition, H3K27me3 and EZH2 protein expression could predict lymph node metastasis.