A biodegradable drug‐controlled delivery system based on mesoporous manganese dioxide and poly(dopamine)

Li Miaomiao,Cai Wenrong,Jiang Lihua,Li Junyao,Li Shan,Tang Tongtong,Kong Yong 대한화학회 2023 Bulletin of the Korean Chemical Society Vol.44 No.12

Mesoporous manganese dioxide (mMnO 2 ) was first synthesized for the loading of methotrexate (MTX), and then dopamine was in situ polymerized on the surface of the MTX‐loaded mMnO 2 (mMnO 2 ‐MTX) in an alkaline solution to encapsulate the drug in the mesopores of mMnO 2 . Both low pH and glutathione (GSH) can result in the degradation of mMnO 2 and poly(dopamine) (PDA), and thus the delivery of MTX from the mMnO 2 ‐MTX‐PDA can be triggered by low pH and GSH. Near‐infrared (NIR) light‐responsive delivery of MTX can be achieved owing to the outstanding photothermal conversion capability of PDA; on the other hand, the mMnO 2 ‐MTX‐PDA can be utilized for photothermal therapy under the irradiation of NIR light due to the elevated temperature. The results of cytotoxicity test demonstrate that the pH, GSH, and NIR light tri‐responsive drug‐controlled delivery system has excellent biocompatibility, while exhibits pronounced growth inhibition against murine breast tumor cell line 4T1. Mesoporous manganese dioxide (mMnO2) was first synthesized for the loading of methotrexate (MTX), and then dopamine was in situ polymerized on the surface of the MTX-loaded mMnO2 (mMnO2-MTX) in an alkaline solution to encapsulate the drug in the mesopores of mMnO2. Both low pH and glutathione (GSH) can result in the degradation of mMnO2 and poly(dopamine) (PDA), and thus the delivery of MTX from the mMnO2-MTX-PDA can be triggered by low pH and GSH. Near-infrared (NIR) light-responsive delivery of MTX can be achieved owing to the outstanding photothermal conversion capability of PDA; on the other hand, the mMnO2-MTX-PDA can be utilized for photothermal therapy under the irradiation of NIR light due to the elevated temperature. The results of cytotoxicity test demonstrate that the pH, GSH, and NIR light triresponsive drug-controlled delivery system has excellent biocompatibility, while exhibits pronounced growth inhibition against murine breast tumor cell line 4T1.

The Correlation between Thyrotropin and Dyslipidemia in a Population-based Study

Li Lu,Beibei Wang,Zhongyan Shan,Fengwei Jiang,Xiaochun Teng,Yanyan Chen,Yaxin Lai,Jiani Wang,Haibo Xue,Sen Wang,Chenyan Li,He Liu,Ningna Li,Jiashu Yu,Liangfeng Shi,Xin Hou,Qian Xing,Xue Bai,Weiping Te 대한의학회 2011 Journal of Korean medical science Vol.26 No.2

This study investigated the relationship between serum thyrotrophin levels and dyslipidemia in subclinical hypothyroid and euthyroid subjects. A total of 110 subjects with subclinical hypothyroidism and 1,240 euthyroid subjects enrolled in this study. Patients with subclinical hypothyroidism had significantly lower high density lipoprotein cholesterol (HDL-C) levels than those who were euthyroid. The lipid profiles were each categorized and mean thyrotrophin levels were higher in subjects in the dyslipidemia subclasses than subjects in the normal subclasses. Thyrotrophin was positively associated with serum triglyceride and negatively associated with serum HDL-C in women. Thyrotrophin was also positively associated with total cholesterol (TC) in the overweight population along with TC and LDL-C in overweight women. In the euthyroid population, thyrotrophin was positively associated with TC in the overweight population. In conclusion, serum thyrotrophin was correlated with dyslipidemia in subclinical hypothyroid and euthyroid subjects; the correlation was independent of insulin sensitivity.

Effects of G-Rh2 on mast cell-mediated anaphylaxis via AKT-Nrf2/NF-κB and MAPK-Nrf2/NF-κB pathways

Chang Xu,Liangchang Li,Chongyang Wang,Jingzhi Jiang,Li Li,Lianhua Zhu,Shan Jin,Zhehu Jin,Jung Joon Lee,Guanhao Li,Guanghai Yan 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.4

Background: The effect of ginsenoside Rh2 (G-Rh2) on mast cell-mediated anaphylaxis remains unclear. Herein, we investigated the effects of G-Rh2 on OVA-induced asthmatic mice and on mast cell-mediatedanaphylaxis. Methods: Asthma model was established for evaluating airway changes and ear allergy. RPMCs and RBL-2H3 were used for in vitro experiments. Calcium uptake, histamine release and degranulation weredetected. ELISA and Western blot measured cytokine and protein levels, respectively. Results: G-Rh2 inhibited OVA-induced airway remodeling, the production of TNF-a, IL-4, IL-8, IL-1b andthe degranulation of mast cells of asthmatic mice. G-Rh2 inhibited the activation of Syk and Lyn in lungtissue of OVA-induced asthmatic mice. G-Rh2 inhibited serum IgE production in OVA induced asthmaticmice. Furthermore, G-Rh2 reduced the ear allergy in IgE-sensitized mice. G-Rh2 decreased the earthickness. In vitro experiments G-Rh2 significantly reduced calcium uptake and inhibited histaminerelease and degranulation in RPMCs. In addition, G-Rh2 reduced the production of IL-1b, TNF-a, IL-8, andIL-4 in IgE-sensitized RBL-2H3 cells. Interestingly, G-Rh2 was involved in the FcεRI pathway activation ofmast cells and the transduction of the Lyn/Syk signaling pathway. G-Rh2 inhibited PI3K activity in adose-dependent manner. By blocking the antigen-induced phosphorylation of Lyn, Syk, LAT, PLCg2, PI3KERK1/2 and Raf-1 expression, G-Rh2 inhibited the NF-kB, AKT-Nrf2, and p38MAPK-Nrf2 pathways. However, G-Rh2 up-regulated Keap-1 expression. Meanwhile, G-Rh2 reduced the levels of p-AKT,p38MAPK and Nrf2 in RBL-2H3 sensitized IgE cells and inhibited NF-kB signaling pathway activation byactivating the AKT-Nrf2 and p38MAPK-Nrf2 pathways. Conclusion: G-Rh2 inhibits mast cell-induced allergic inflammation, which might be mediated by theAKT-Nrf2/NF-kB and p38MAPK-Nrf2/NF-kB signaling pathways

Novel impedance matching method based on negative resistors for WPT

Li, Yang,Liu, Jiaming,Jiang, Shan,Ni, Xin,Ma, Jingnan,Wang, Rui,Sha, Lin The Korean Institute of Power Electronics 2020 JOURNAL OF POWER ELECTRONICS Vol.20 No.4

In a wireless power transfer (WPT) system via coupled magnetic resonance, the transmission power and efficiency dramatically decrease with increased in the transfer distance and load variations. In the conventional impedance matching method, different resistors are matched by adjusting passive devices such as capacitors and inductors. The corresponding matching range changes with different topological network structures. In particular, there exists a matching forbidden zone. In this paper, a novel impedance matching method based on a negative resistor is proposed to match the impedance in the forbidden zone. First, the conventional impedance matching network is analyzed based on circuit theory. Then, the feasibility of the proposed method is verified. In addition, the receiving power obtained using the conventional matching method is compared with that obtained using the proposed method. It is theoretically verified that the novel method can break the upper power limit related to the conventional method, which realizes a higher receiving power. A prototype was built in the laboratory. Experimental results show the feasibility of the novel method. Moreover, derived simulation results were basically consistent with the experimental values, which also demonstrates the validity of the proposed method.

A novel CHD7 variant in a chinese family with CHARGE syndrome

Shan Yanhong,Yao LingFang,Li Linli,Gao Xueping,Jiang Jinghan 한국유전학회 2024 Genes & Genomics Vol.46 No.3

Objective CHARGE syndrome is a rare autosomal dominant (AD) multi-system disorder with a broad and variable clinical manifestation and occurs in approximately 1/10,000 newborns in the world. Mutations in the CHD7 gene are the genetic cause of over 90% of patients with typical CHARGE syndrome. The present study reported a novel variant in the CHD7 gene in a Chinese family with an abnormal fetus. Methods Routine prenatal ultrasound screening showed fetal heart abnormality and left foot varus. Chromosomal microarray analysis (CMA) and fetus-parent whole-exome sequencing (trio-WES) were performed to determine the genetic cause of the fetus. The candidate variant was further verified using Sanger sequencing. Results CMA analysis revealed normal results. However, WES analysis identified a de novo heterozygous variant of c.2919_2922del (NM_017780.4) on exon 11 of CHD7 gene, resulting in a premature truncation of the CHD7 protein (p.Gly975*). The variant was classified as Pathogenic (PVS1 + PS2_Moderate + PM2_Supporting) based on the ACMG guidelines. Combined with the clinical phenotype of fetal heart abnormalities, it was confirmed CHARGE syndrome. Conclusion We identified a novel heterozygous variant c.2919_2922del in CHD7 of a Chinese fetus with CHARGE syndrome, enriching the genotype-phenotype spectrum of CHD7. These results suggest that genetic testing could help facilitate prenatal diagnosis of CHARGE syndrome, thus promoting the appropriate genetic counseling. Objective CHARGE syndrome is a rare autosomal dominant (AD) multi-system disorder with a broad and variable clinical manifestation and occurs in approximately 1/10,000 newborns in the world. Mutations in the CHD7 gene are the genetic cause of over 90% of patients with typical CHARGE syndrome. The present study reported a novel variant in the CHD7 gene in a Chinese family with an abnormal fetus. Methods Routine prenatal ultrasound screening showed fetal heart abnormality and left foot varus. Chromosomal microarray analysis (CMA) and fetus-parent whole-exome sequencing (trio-WES) were performed to determine the genetic cause of the fetus. The candidate variant was further verified using Sanger sequencing. Results CMA analysis revealed normal results. However, WES analysis identified a de novo heterozygous variant of c.2919_2922del (NM_017780.4) on exon 11 of CHD7 gene, resulting in a premature truncation of the CHD7 protein (p.Gly975*). The variant was classified as Pathogenic (PVS1 + PS2_Moderate + PM2_Supporting) based on the ACMG guidelines. Combined with the clinical phenotype of fetal heart abnormalities, it was confirmed CHARGE syndrome. Conclusion We identified a novel heterozygous variant c.2919_2922del in CHD7 of a Chinese fetus with CHARGE syndrome, enriching the genotype-phenotype spectrum of CHD7. These results suggest that genetic testing could help facilitate prenatal diagnosis of CHARGE syndrome, thus promoting the appropriate genetic counseling.

Application of High-speed Counter-current Chromatography for the Isolation of 5 Alkaloids from Lotus (Nelumbo nucifera Gaertn.) Leaves

Jiang-Ning Hu,Bin Shan,Ze-Yuan Deng,Jing Li,Ya-Wei Fan,Rong Liu,Zheng Ruan 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.6

A high-speed counter-current chromatography (HSCCC) method was developed for isolation of 5alkaloids from lotus (Nelumbo nucifera Gaertn.) leaves. The 2-phase solvent system of HSCCC composed of light petroleum-ethyl acetate-methanol-water was set up in 2-step separation process in the proportion of 3:5:3:5 for isolation of N-nornuciferine and armepavine, and in the proportion of 1:5:1:5 for that of anonaine, pronuciferine,and nuciferine. The purity of anonaine (14.6mg), pronuciferine (29.7 mg), N-nornuciferine (31.4 mg), nuciferine (22.1 mg),and armepavine (23.3 mg) isolated from 150 mg crude extract of lotus leaves were examined as 95.6, 88.2, 92.5,94.3, and 92.1%, respectively.

Novel double-walled microspheres based on chitosan, sodium cellulose sulfate and sodium tripolyphosphate: Preparation, characterization and in vitro release study

Li-Ying Zhu,Xiao-Qin Yan,Hong-Man Zhang,Shan-Jing Yao,Ling Jiang 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.3

A novel double-walled microsphere composed of chitosan, sodium cellulose sulfate (NaCS) and sodiumtripolyphosphate (TPP) was prepared. TPP was used as ionic crosslinker. The morphology of the microspheres wasobserved by microscope and SEM, and the results showed that the double-walled microsphere was smooth outside,with rough interior surface. FTIR spectra analysis was performed to investigate the PEC formation among chitosan,NaCS, and TPP. In vitro release studies of BSA showed that the double-walled microspheres had regular and sustainablerelease profiles in simulated colonic fluid (SCF). Our results indicated that the double-walled microspheres preparedcould be used as a candidate protein drug carrier for the colon.

Elevated Mean Platelet Volume is Associated with Presence of Colon Cancer

Li, Jia-Ying,Li, Ying,Jiang, Zheng,Wang, Rui-Tao,Wang, Xi-Shan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Background: Colon cancer is the second most common cancer in developed countries. Activated platelets play a key role in inflammation and atherothrombosis, with mean platelet volume (MPV) is an early marker of platelet activation. The aim of the study was to clarify the relevance of MPV in patients with colon cancer. Materials and Methods: We measured MPV levels in 128 patients with colon cancer before and after surgery, and 128 controls matched for age, gender, body mass index (BMI) and smoking status. The odds ratios (ORs) and 95% confidence intervals (CIs) for colon cancer were calculated using multivariate logistic regression analyses across MPV quartiles. Results: Patients with colon cancer had higher MPV compared with controls. Surgical tumor resection resulted in a significant decrease in MPV levels (11.4 fL vs 10.7 fL; p<0.001). A positive correlation between MPV and tumor-nodule-metastases (TNM) stage was found. Furthermore, after adjusting for other risk factors, the ORs (95%CIs) for colon cancer according to MPV quartiles were 1.000, 2.238 (1.014-4.943), 3.410 (1.528-7.613), and 5.379 (2.372-12.198), respectively. Conclusions: The findings show that patients with colon cancer have higher MPV levels compared with controls, and these are reduced after surgery. In addition, MPV was found to be independently associated with the presence of colon cancer. Further studies are warranted to assess the utility of MPV as a novel diagnostic screening tool for colon cancer.

Protective Effect of Schisandra chinensis Polysaccharides Against the Immunological Liver Injury in Mice Based on Nrf2/ARE and TLR4/NF-κB Signaling Pathway

Yingying Shan,Bin Jiang,Jiahui Yu,Jiaye Wang,Xiaoli Wang,He Li,Chunmei Wang,JianGuang Chen,Jinghui Sun 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.9

We have previously demonstrated the hepatoprotective effect of Schisandra chinensis polysaccharides (SCP) against the liver injury induced by alcohol, high-fat diet, and carbon tetrachloride in mice. In this study, we investigated the effect of SCP against the immunological liver injury induced by concanavalin A (Con A) in mice. The results showed that SCP could significantly reduce the level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in the serum of mice with immunological liver injury. SCP could significantly decrease the content of malondialdehyde (MDA) and nitric oxide (NO) and increase the activity of superoxide dismutase (SOD) and glutathione (GSH) in the liver tissue. SCP could significantly increase the number of CD4+ and decrease the number of CD8+ in the peripheral blood, and elevate the ratio of CD4+/CD8+. SCP could significantly downregulate the expression of Kelch-like ECH-associated protein 1 (Keap1) and upregulate the expression of nuclear factor-erythroid 2-related factor2 (Nrf2) and downstream gene heme oxygenase-1 (HO-1), and downregulate the expression of toll-like receptor 4 (TLR4), myeloid differentiation primary response gene 88 (MyD88), and nuclear factor-kappa B (NF-κB) proteins. This study indicates that SCP can reduce the release of a large number of inflammatory factors to inhibit the oxidative stress in mice with the immunological liver injury induced by Con A, and its mechanism is closely related to the regulation of Nrf2/antioxidant response element and TLR4/NF-κB signaling pathways.

Residual Stress Distribution in Hard-Facing of Pressure Relief Valve Seat

Ai, Li,Yu, Xinhai,Jiang, Wenchun,Woo, Wanchuck,Ze, Xiaofeng,Tu, Shan-Tung AMERICAN SOCIETY MECHANICAL ENGINEERS 2014 Journal of Pressure Vessel Technology Vol.136 No.6

<P>In this study, for the hard-facing of spring-loaded pressure relief valve seats, the residual stress distributions after the tungsten inert gas welding, (TIG) postwelded heat treatment and subsequent surface turning were investigated. The heat input parameters of welding were calibrated using an infrared imaging and thermocouples. The residual stress distributions were computed using three-dimensional finite element model. The neutron diffraction approach was employed to verify the finite element calculation. It is found that, the surface temperature during hard-facing welding shows a double ellipsoidal shape with the highest value of around 1570 degrees C. The high residual stress zones are located exactly under the welded joint except a slight deviation in the hoop direction. The magnitudes of tensile residual stresses in the three directions increase with their corresponding locations from the root of the joint into the base metal. The residual stresses in all of the three directions decrease significantly after the heat treatment. After surface turning, the residual stresses are tensile except for those close to the inner surface that are compressive in axial and radial directions.</P>