黃連解毒湯이 Collagenase에 의한 흰쥐의 뇌출혈 및 뇌부종에 미치는 영향

구자승(Ja-Seung Ku),이준석(Joon-Suk Lee),신정원(Jung-Won Shin),김성준(Seong-Joon Kim),손낙원(Nak-Won Sohn) 대한한의학회 2011 대한한의학회지 Vol.32 No.5

황련해독탕의 피부지방장벽개선을 통한 Th2 분화조절이 아토피피부염 완화에 미치는 효과

손성한,안상현,박선영,김기봉,Son, Seong Han,Ahn, Sang Hyun,Park, Sun-Young,Kim, Kibong 대한한방소아과학회 2018 대한한방소아과학회지 Vol.32 No.3

Objectives Hwangnyeonhaedok-tang is a Korean herbal medical treatment that removes toxic heat, fever and inflammation. The purpose of this study was to investigate the effect of Hwangnyeonhaedok-tang treatment on the relief of atopic dermatitis (AD) through regeneration of skin lipid barrier. Methods Male NC/Nga mice (20 g, 6 week age) were used. Each 10 mice were allocated to the control group (Ctrl), the AD-induced with no treatment group (AE), and the group which induced AD after administering Hwangnyeonhaedok-tang extract (HT). To induce AD-like skin lesions, sodium dodecyl sulfate (SDS) (Sigma-Aldrich, USA) was rubbed on the back of each mouse to remove the lipid lamella of the stratum corneum, and Dermatophagoides (D.) farinae crude extract was applied. HT group was orally administered Hwangnyeonhaedok-tang after induction of AD. IL-4 IL-13, $p-I{\kappa}B$, iNOS, Sudan Black B (SB), loricrin, and filaggrin were observed to confirm the effect. Results In HT group, AD skin score was decreased by 46%. The cytokine IL-4 and IL-13, which can identify Th2 differentiation, was reduced by 73% and 58% each. Anti-inflammatory effects were observed in $p-I{\kappa}B$ and iNOS by 69% and 54%, respectively. Finally, SB showed that the regeneration of the lipid layer and the increase of the regeneration power of loricrin and filaggrin were increased by 437% and 464%, respectively. Conclusions From the study result, we observed that Hwangnyeonhaedok-tang treatment alleviates AD by decreasing skin score, reducing Th2 differentiation, inducing anti-inflammatory, and increasing skin lipid barrier regeneration. Thus, Hwangnyeonhaedok-tang treatment would be considered as an effective AD relieving treatment.

전신 근간대성 경련을 호소하는 정신성 비간질성 경련 환자에 대한 억간산 및 황련해독탕 병용 투여 치험 1례

배인후,하원정,서유나,이현규,김수현,조기호,문상관,정우상,권승원,진철,Bae, In hu,Ha, Won Jung,Seo, Yuna,lee, Hyun-kue,Kim, Su hyeon,Cho, Ki-Ho,Moon, Sang-Kwan,Jung, Woo-Sang,Kwon, Seungwon,Jin, Chul 대한중풍순환신경학회 2020 대한중풍.순환신경학회지 Vol.21 No.1

■ Objectives This case report is reported to show the effect of Ukgansan and Hwangnyeonhaedok-tang on a patient who complaining myoclonic seizure due to psychogenic non epileptic seizure, occurred after traumatic stress event. ■ Methods A female patients with myolconic seizure was treated with Ukgansan and Hwangnyeonhaedok-tang during 5 days. Then we assessed dyskinesia severity by AIMS(Abnormal Involuntary Movement Scale) every two days til discharge. ■ Results Seizure decreased two days after hospitalization, then disappeared third day. There was no recurrence till discharge. When she revisited outpatients clinic, there was no symptom. ■ Conclusion This case showed the effect of Ukgansan and Hwangnyeonhaedok-tang on psychogenic epileptic seizure.

중등도의 불면환자를 六味地黃湯(Liuwei Dihuang decoction)과 黃連解毒湯(Huanglian Jiedu Decoction) 병용 투여 및 배수혈 전침치료로 호전시킨 1례 : 증례보고

노민영,이지현,전형선,한양희,김현호,임정태 대한한방내과학회 2020 大韓韓方內科學會誌 Vol.41 No.3

Objectives: Insomnia is a common condition that causes deterioration of cognitive function and physical function and has a negative impact on quality of life. Insomnia is experienced by more than one-third of the population of the world. Although sleeping pills are currently used as therapeutic agents, they have side effects, so safer treatment methods are needed. Therefore, we report a case of an outpatient who sleeps only one hour a day due to insomnia. Methods: The patient visited Korean Medicine Hospital 10 times for four weeks and was treated with Korean medicine therapies, including electroacupuncture on the back-Shu points and herbal medicine (Yukmijihwang-tang and Hwangnyeonhaedok-tang). Treatment progress was assessed using the Pittsburgh Sleep Quality Index (PSQI) and the Insomnia Severity Index (ISI). Results: After 29 days of treatment, the PSQI score decreased from 20 points and 14 points. The ISI score also decreased to decreased to 22 points from 26 points. The average daily sleeping time increased from 60 minutes to 197.1 minutes after treatment. No significant side effects were observed. Conclusions: Yukmijihwang-tang and Hwangnyeonhaedok-tang with electroacupuncture might be a recommended therapeutic option for insomnia patients.

황련해독탕 및 발효황련해독탕의 항염증 효과

양현주,주현아,백상철,박진수,홍석훈,Yang, Hyun-Ju,Joo, Hyun-A,Baek, Sang-Chul,Park, Jin-Soo,Hong, Seok-Hun 대한한방안이비인후피부과학회 2011 한방안이비인후피부과학회지 Vol.24 No.2

Objective : Recently interest of fermented herb medicine has increased in Oriental medicine. Herb medicine fermented in a proper condition resultingly has better or new virtue by microbe. This study was designed to compare the anti-inflammatory effects of herbal decoction and fermented herbal decoction in Hwangnyeonhaedok-tang (Huanglian fiedu-tang; HHT). Methods : The effects of HHT and FHHT on anti-inflammation were measured by production of NO and pro-inflammatory cytokine productions including TNF-${\alpha}$ and IL-1${\beta}$, IL-6 in LPS induced RAW 264.7 cells. Results : 1. Cell viability in three doses of HHT extract (0.01, 0.05 and 0.10 mg/ml) and two doses of FHHT extract (0.01, 0.05 mg/ml) was more than 70% and 80% each. 2. In RAW 264.7 cells, LPS displayed significant stimulatory effects on the production of NO. However, both HHT, FHHT showed significant inhibitory effects on the production of NO. 3. Pre-treatment by HHT in a various concentrations (0.01, 0.05, 0.10, 0.50mg/ml) reduced the elevated production of TNF-${\alpha}$, IL-1${\beta}$, IL-1${\beta}$ mRNA, IL-6 mRNA. More than 0.05mg/ml concentrations of HHT reduced the production of IL-6. And TNF-${\alpha}$, IL-1${\beta}$ level of mouse serum reduced in a various dose(0.1, 0.5, 1.0g/kg). 4. Pre-treatment by FHHT in a various concentrations (0.01, 0.05, 0.10, 0.50mg/ml) reduced the elevated production of TNF-${\alpha}$. More than 0.05mg/ml concentrations of FHHT reduced the expression of TNF-${\alpha}$ mRNA. 0.1, 0.50mg/ml concentrations of FHHT reduced the production of IL-6. And IL-1${\beta}$ level of mouse serum reduced in a various dose(0.1, 0.5, 1.0g/kg). Conclusions : These results indicated that both HHT and FHHT could have anti-inflammatory capacity by inhibiting the production of NO and cytokines in vitro. But HHT has more strong anti-inflammatory effects than that of FHHT in broad outlines.

황련해독탕(黃連解毒湯)과 건강부자탕(乾薑附子湯)의 Cold Stress로 유발된 생쥐의 혈중(血中) 호르몬 농도변화에 대한 비교연구

한상용,강한주,최은식,이기남,이태희,김윤경,Han, Sang-Yong,Kang, Han-Ju,Choi, Eun-Sik,Lee, Ki-Nam,Lee, Tae-Hee,Kim, Yun-Kyung 대한한의학방제학회 2013 大韓韓醫學方劑學會誌 Vol.21 No.2

Objectives : The aim of this study was to evaluate the efficacy of Hwangnyeonhaedok-tang (HH) and Geongangbuja-tang (GB) on the plasma hormone level in mice exposed to cold stress. HH and GB are the representative prescriptions of cold and hot property, respectively. Methods : We established cold condition by confining ICR mice to a $4^{\circ}C$ cage for 24 hours, ICR mice were given a HH (100, 300, 1000 mg/kg) or GB (100, 300, 1000 mg/kg) extract orally twice a day for three consecutive days. From the second day, they were given cold stress ($4^{\circ}C$) for twenty four hours. To measure the plasma corticosterone, insulin, thyroxine, epinephrine and norepinephrine levels of mice, their blood samples were collected from cardiac puncture, immediately centrifuged at $4^{\circ}C$. The protein level of HSP70 and JNK was examined using western blot analysis in cortex and hypothalamus. Results : Oral administration of GB more significantly reduced plasma corticosterone level raised by cold stress than HH. Gardeniae Fructus (CJ), the constituent of HH, significantly increased the thyroxine level. Western blot analysis showed that cold stress-induced Heat shock protein 70 (HSP70) expression was increased by HH and GB, HH decreased JNK expression and GB increased JNK expression dose-depently in hypothalamus. Scutellariae Radix (HG), Zingiberis Rhizoma (GG) and Aconiti Tuber (BJ) decreased HSP70 in hypothalamus and GG, BJ decreased HSP70 in cortex as well. Conclusions : These results suggest Geongangbuja-tang (GB) is more effective for ameliorating the stress response caused by cold stress.

시판 황련해독탕 Ex제제의 성분함량 모니터링 연구

김은정,박혜정,김현주,김주호,안지영,이정호,김윤경,Kim, Eun-Jeong,Park, Hye-Jung,Kim, Hyun-Ju,Kim, Ju-Ho,Ann, Ji-Young,Lee, Jeong-Ho,Kim, Yun-Kyung 대한한의학방제학회 2008 大韓韓醫學方劑學會誌 Vol.16 No.1

Objectives : Hwangnyeonhaedok-tang(HNHDT) is the one of well-known traditional herbal formula and has been widely used for the treatment of various diseases in Korea. For the quality improvement of commercial traditional herbal formula, we investigated the quality of prepared HNHDT. Methods: We measured the contents of baicalin, berberine and geniposide in the HNHDT Ex preparations on the market by HPLC and compared with the official notice of ingredients. Results: In 3 preparations of HNHDT covered with the national health insurance(NHI), the content of baicalin was various from 32.83mg to 185.05mg, the total berberine was $12.0{\sim}50.75mg$ and geniposide was $12.57{\sim}44.84mg$. Contents of marker ingredients in NHI Preparations were below the standard and showed a large variation. In 7 non NHI Preparations of HNHDT and the standard(STD) HNHDT manufactured in laboratory, the content of baicalin was $37.16{\sim}115.69mg$, the total berberine was $5.62{\sim}28.43mg$ and geniposide was $10.98{\sim}113.47mg$. But, because composition of non-NHI preparations is different from NHI preparations, every maker's contents were up to the notice for baicalin and berberine. There was newly enforced standard regulations of geniposide in non-NHI preparations, so contents of geniposide was up to that standard only in JI and STD. Conclusions: There was a large variation of contents in the HNHDT Ex preparations on the market and the quality differences were existed between NHI and non-NHI preparations of HNHDT. Further studies for evaluation of efficacy differences and standardization of manufacturing processes are necessary.

황련해독탕(黃連解毒湯)의 아토피 피부염 개선 효과(效果)

기호필,장선일,윤용갑,Ki, Ho-Pil,Jang, Seon Il,Yun, Young-Gab 대한한의학방제학회 2013 大韓韓醫學方劑學會誌 Vol.21 No.1

Objectives : The water extract of Hwangnyeonhaedok-tang (HHT), composed of the Scutellariae Radix, Coptidis Rhizoma, Phellodendri Cortex and Gardeniae Fructus has been traditionally used to treat fever, inflammation, gastritis and hypertension in east asia. However, little is known about the ameliorative effects of HHT on atopic dermatitis. Therefore, the purpose of this study was to investigate the therapeutic effect of HHT on atopic dermatitis Methods : We investigated the inhibitory effects of HHT on the production of proinflammatory cytokines in rat peritoneal mast cells (RPMCs), on the scratching behavior in ICR mice, and on atopic dermatitis symptoms in 2,4-dinitrofluorobenzene (DNFB)-induced atopic dermatitis-like model hairless mice. Results : Levels of TNF-${\alpha}$ and IL-$1{\beta}$ increased by PMA plus A23187 co-treatment were significantly inhibited by HHT in a dose-dependent manner. HHT also inhibited the histamine release from RPMCs stimulated by compound 48/80, which promotes histamine release. The oral administration of HHT reduced the scratching behavior induced by compound 48/80 and histamine in ICR mice. Furthermore, the intradermal treatment of HHT reduced the ear edema, skin lesions, and atopic molecular marker (IgE and IL-4) in DNFB-induced atopic dermatitis model mice. Conclusions : These results suggest that HHT may be used as a potential treatment for AD as a prescription for treatment of atopic dermatitis.

LPS로 활성화된 대식세포에서 황련해독탕(黃連解毒湯)물추출물의 염증매개물질 억제효과

김대희 ( Dae Hee Kim ),박숙자 ( Sook Jahr Park ),정지윤 ( Ji Yoon Jung ),김상찬 ( Sang Chan Kim ),변성희 ( Sung Hui Byun ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.4

Objectives: Hwangnyeonhaedok-tang (Huanglian Jiedu Tang; HHT) has been widely used for purging `fire` and lessening virulence of any pathogenic organism. However it has been rarely conducted to evaluate the immuno-biological activity. In this study, we evaluated anti-inflammatory effects of HHT in LPS-activated Raw264.7 cells. Methods: Cells were treated with 1 μg/ml of LPS 1 h prior to the addition of HHT. Cell viability was measured by MTT assay. The production of NO was determined by reacting cultured medium with Griess reagent. PGE2 and proinflammatory cytokines were detected by ELISA. Expression of iNOS, COX-2, IκBα and NF-κB were analyzed by immunoblot analysis. Results: All three doses of HHT (0.03, 0.10 and 0.30 mg/ml) had no significant cytotoxicity during the entire experimental period. The levels of NO and PGE2 were dramatically augmented by LPS compared to control. However, HHT extract dose-dependently reduced these increases. Expression of iNOS and COX-2 protein were also decreased by treatment with HHT extract. Furthermore, HHT extract significantly reduced the nuclear translocation of NF-κB which is critical in regulating inflammation through transcription of iNOS and COX-2. In addition, HHT extract reduced the elevated production of inflammatory cytokines including TNF-α, IL-1β and IL-6. Conclusions: The results in this study demonstrate that HHT extract exerts anti-inflammatory activities through the inhibition of NO, PGE2 and proinflammatory cytokines production via the suppression of NF-κB.

황련해독탕의 유해미생물에 대한 항균 효과

하성호 ( Seong Ho Ha ),박정아 ( Chung A Park ),박숙자 ( Sook Jahr Park ) 대구한의대학교 제한동의학술원 2018 東西醫學 Vol.43 No.2

Objective : Hwangnyeonhaedok-tang (HHT) has been used to reduce heat caused by the pathogenic substances and to treat influenza, menopausal syndrome, stomatitis, and toothache. In this study, the effects of HHT on the growth of oral bacteria and acne bacteria were evaluated. Methods : HHT was prepared by extracting 160 g of dry sample in 2ℓ of water for 3 h. The antibacterial potential of HHT was determined by the agar diffusion method and minimum inhibitory concentration (MIC) assay. Results : HHT showed strong antibacterial activity against Streptococcus mutan s and Propionibacterium acnes. At the highest amount of HHT tested (2 ㎎/disk), the mean diameter of the clear zone was recorded at 13 ㎜ for S. mutans and 27.5 ㎜ for P. acnes. MIC values for HHT against S. mutans and P. acnes were recorded as 250 and 62.5 ㎍/㎖, respectively. In addition, Phellodendri cortex showed potent antibacterial activity when comparing antimicrobial activity of Scutellariae radix, Coptidis rhizoma and Phellodendri cortex among herbs constituting HHT. Conclusion : The results of the present study suggest that HHT has antibacterial activity against human pathogenic bacterial strains and indicate possible use of HHT as antibacterial agents.