Negative Regulation of Pathogenesis in Pseudo-monas syringae pv. tabaci 11528 by ATP-Dependent Lon Protease

양현주,이준승,차지영,백형석 한국분자세포생물학회 2011 Molecules and cells Vol.32 No.4

Pseudomonas syringae pv. tabaci causes wildfire disease in tobacco plants. The hrp pathogenicity island (hrp PAI) of P. syringae pv. tabaci encodes a type III secretion system (TTSS) and its regulatory system, which are required for pathogenesis in plants. Three important regulatory proteins-HrpR, HrpS, and HrpL-have been identified to activate hrp PAI gene expression. The bacterial Lon protease regulates the expression of various genes. To investigate the regulatory mechanism of the Lon protease in P. syringae pv. tabaci 11528, we cloned the lon gene, and then a Δlon mutant was generated by allelic exchange. lon mutants showed increased UV sensitivity, which is a typical feature of such mutants. The Δlon mutant produced higher levels of tabtoxin than the wild-type. The lacZ gene was fused with hrpA promoter and activity of β-galactosidase was measured in hrp-repressing and hrp-inducing media. The Lon protease functioned as a negative regulator of hrp PAI under hrp-repressing conditions. We found that strains with lon disruption elicited the host defense system more rapidly and strongly than the wild-type strain,suggesting that the Lon protease is essential for systemic pathogenesis.

Usefulness of the Berlin Questionnaire for Screening Patients With Obstructive Sleep Apnea

양현주,유성희 대한수면연구학회 2022 Journal of sleep medicine Vol.19 No.3

Objectives: This study aimed to evaluate the usefulness of the Berlin questionnaire (BQ) as a screening tool for obstructive sleep apnea (OSA) in a sleep clinic.Methods: We used a retrospective review of 77 subjects with suspected OSA to conduct a secondary analysis of a previously published sleep study. A total of 77 subjects attended and completed overnight, in-laboratory polysomnography. Subjects completed the standard BQ in the evening just before the sleep study.Results: The mean age of 77 subjects was 49.94±15.78 years, of which 37 (48.1%) were male and 42 (63.7%) were white. Forty-six subjects (59.7%) were diagnosed with OSA through polysomnography. In the analysis of each item of the standard BQ, the sensitivity ranged from 4.6% to 92.5%, and the specificity ranged from 13.3% to 85.7%. For item 8, the sensitivity, specificity, positive likelihood ratio, and negative likelihood ratio were 4.6%, 84.9%, 0.3, and 1.1, respectively. The area under the curve values of the standard BQ and after deleting item 8 were 0.634 and 0.751, respectively. When item 8 was deleted and each item on the standard BQ was calculated as one point, the cutoff values representing the highest Yuden index were 3.5 and 4.5.Conclusions: A modified BQ that selects four different questions for each subject, regardless of the number of positive categories in the standard BQ, will provide improved accuracy in screening subjects with a high likelihood of having OSA.

국제선 항공사 기내서비스에 대한 불만족과 불평행동유형에 관한 연구

양현주 한국관광정책학회 2003 觀光政策學硏究 Vol.9 No.2

The deepening of competition in the airline industry made the motive to evoke the quality management which emphasizes the quality side in the airline service. Espesially, in the service the fact that the customer's loyalty extent is desided buy how the dissatisfaction than satisfaction is dissolved, the complaint which customers feel is controlled and deserved and repurchase intention become higher means the treatment of complaint behavior of dissatisfied customers became key factor in the customer management. Customers have five fundamental alternatives when they have dissatisfied with a purchase or some aspect of it; take no action, discontinue use of the service, tell friends and others about their problem, complain to cabin manager, complain to sort of consumer protection group and KNTO(Korea National Tourism Organization) For the reserch, this study collected 240 questionnaires targeted to native passengers who come to Korea at the Inchon international airport terminal and set up five hypothesis and used muitiful regression analysis, paired-sample T-test, crosstabulations analysis. According to survey, 1st, Evaluation factor of cabin service quality is positively related to customer's satisfaction. 2nd, There is significant difference between the attribution of cabin service quality and subject of dissatisfaction. Specially, They are dissatisfied by the human professionalism such as cabin crew's clean appearance, courteous cabin crew, pertinent service procedures. 3rd, Dissatisfied passengers than satisfied passengers show strong complaint behavior. 4th, Rutine object passengers than object passengers show strong complaint behavior. 5th, Dissatisfied passengers using national airlines were more likely to refuse to use them again than those using foreign airlines. As a result of the reserch, it could be helpful for the airline industry to establish marketing strategy by understanding dissatisfied factors and complaining behaviors of airline passengers. Finally, Airline management should make more efforts to improve service quality.

A Case of Methanol Poisoning Treated with Continuous Renal Replacement Therapy - The Serial Measurements of Serum Methanol Concentrations in Methanol Poisoning -

양현주,채수완,노순옥,정윤조,오성식,최미림,이종화,최명우,윤현주,선인오,이광영 대한응급의학회 2016 대한응급의학회지 Vol.27 No.5

Methanol poisoning is a medical emergency that requires rapid elimination of the toxin and its metabolites for recovery. The danger of methanol results from the accumulation of its toxic metabolite formic acid. This accumulation may result in the development of metabolic acidosis, visual impairment, and damage to the basal ganglia. Extracorporeal treatment is recommended in severe cases of methanol poisoning with coma, seizure, new vision deficits, metabolic acidosis, high serum anion gap, elevated methanol concentrations or impaired kidney function. Although the serum methanol concentration is helpful in determining the use of extracorporeal treatment, methanol assays are not standard laboratory tests in Korea. Herein, we report a case of methanol poisoning in which the patient’s clinical improvement was confirmed using serum and urine methanol levels.

Is there regional inequality in the medical accessibility of the severely injured? Application of driving time data in South Korea

양현주,Elliot Kang,김민경,고강혁 서강대학교 지암남덕우경제연구원 2020 시장경제연구 Vol.49 No.1

An important aspect of successfully treating the severely injured, or trauma patients, is accessibility to a hospital within an hour (the “golden hour”). In this study, we use novel driving time data to investigate the regional disparity in accessing trauma centers in 3,400 townships in South Korea. We found that 99.8% of residents in metropolitan cities were located within a 60-minute drive to trauma centers, whereas 21% of residents (6 million) in non-metropolitan cities could not reach trauma centers within 60 minutes. The results suggest that government resources should be prioritized toward improving trauma center accessibility in non-metropolitan regions.

Application of Lemongrass Oil-Containing Polylactic Acid Films to the Packaging of Pork Sausages

양현주,송경빈 한국축산식품학회 2016 한국축산식품학회지 Vol.36 No.3

Polylactic acid (PLA) is a biodegradable and renewable polymer, which represents a valuable alternative to plastic packaging films, often associated with environmental problems. In this study, we tested the suitability of PLA as a biodegradable packaging film and assessed the antimicrobial activity of lemongrass oil (LO), incorporated into the PLA film in different concentrations. To obtain the optimal physical properties for PLA films, tensile strength, elongation at break, and water vapor permeability were measured under different preparation conditions. In addition, the antimicrobial activity of the LO contained in the PLA film against Listeria monocytogenes was investigated by disc diffusion and viable cell count. Among all concentrations tested, 2% LO was the most suitable in terms of antimicrobial activity and physical properties of the PLA film. Based on these results, we used the PLA film containing 2% LO to pack pork sausages; after 12 d of storage at 4°C, the population of inoculated L. monocytogenes in the sausage samples wrapped with the PLA film containing 2% LO was reduced by 1.47 Log CFU/g compared with the control samples. Our data indicate that PLA films containing 2% LO represent a valuable means for antimicrobial sausage packaging.

황련해독탕 및 발효황련해독탕의 항염증 효과

양현주,주현아,백상철,박진수,홍석훈,Yang, Hyun-Ju,Joo, Hyun-A,Baek, Sang-Chul,Park, Jin-Soo,Hong, Seok-Hun 대한한방안이비인후피부과학회 2011 한방안이비인후피부과학회지 Vol.24 No.2

Objective : Recently interest of fermented herb medicine has increased in Oriental medicine. Herb medicine fermented in a proper condition resultingly has better or new virtue by microbe. This study was designed to compare the anti-inflammatory effects of herbal decoction and fermented herbal decoction in Hwangnyeonhaedok-tang (Huanglian fiedu-tang; HHT). Methods : The effects of HHT and FHHT on anti-inflammation were measured by production of NO and pro-inflammatory cytokine productions including TNF-${\alpha}$ and IL-1${\beta}$, IL-6 in LPS induced RAW 264.7 cells. Results : 1. Cell viability in three doses of HHT extract (0.01, 0.05 and 0.10 mg/ml) and two doses of FHHT extract (0.01, 0.05 mg/ml) was more than 70% and 80% each. 2. In RAW 264.7 cells, LPS displayed significant stimulatory effects on the production of NO. However, both HHT, FHHT showed significant inhibitory effects on the production of NO. 3. Pre-treatment by HHT in a various concentrations (0.01, 0.05, 0.10, 0.50mg/ml) reduced the elevated production of TNF-${\alpha}$, IL-1${\beta}$, IL-1${\beta}$ mRNA, IL-6 mRNA. More than 0.05mg/ml concentrations of HHT reduced the production of IL-6. And TNF-${\alpha}$, IL-1${\beta}$ level of mouse serum reduced in a various dose(0.1, 0.5, 1.0g/kg). 4. Pre-treatment by FHHT in a various concentrations (0.01, 0.05, 0.10, 0.50mg/ml) reduced the elevated production of TNF-${\alpha}$. More than 0.05mg/ml concentrations of FHHT reduced the expression of TNF-${\alpha}$ mRNA. 0.1, 0.50mg/ml concentrations of FHHT reduced the production of IL-6. And IL-1${\beta}$ level of mouse serum reduced in a various dose(0.1, 0.5, 1.0g/kg). Conclusions : These results indicated that both HHT and FHHT could have anti-inflammatory capacity by inhibiting the production of NO and cytokines in vitro. But HHT has more strong anti-inflammatory effects than that of FHHT in broad outlines.

제주 강정마을 주민들의 외상 후 스트레스장애 유병률과 관련 요인

양현주,정영은,박준혁,김문두 대한신경정신의학회 2019 신경정신의학 Vol.58 No.4

Objectives Most studies on post-traumatic stress disorder (PTSD) have involved a small sample size and a specific traumatic event, with few studies reporting on subjects who have been continuously exposed to a traumatic event. Timely assessment and treatment are crucial for individuals chronically exposed to a traumatic event. Therefore, this study investigated the prevalence of PTSD and associated factors in all residents of Gangjeong village, who, recently, have been exposed to a traumatic event for a prolonged period. Methods The subjects of this study were the residents of Gangjeong village, who have been exposed to a traumatic event related to the construction of the Jeju Civilian-Military Complex Port. The survey included items related to general characteristics and PTSD symptoms, which were assessed using the Impact of Event Scale-Revised, Korean version. Results The prevalence of PTSD symptoms was 26.8% (95% confidence interval=23.54–30.04). Multivariate logistic regression analysis identified age, length of residence, and marital status as factors significantly associated with PTSD symptoms. Conclusion The prevalence of PTSD symptoms was higher among the study population than in the general population. Economically active age groups, people exposed to the traumatic event throughout their duration of residence in the village, and unmarried individuals were found to be more likely to develop PTSD symptoms. Mental, social, and financial support should be directed to the affected groups of individuals.

간호대학생의 지각된 스트레스와 대학생활적응의 관계: 대인관계능력의 매개효과

양현주,변은경 국제문화기술진흥원 2023 The Journal of the Convergence on Culture Technolo Vol.9 No.1

본 연구는 간호대학생을 대상으로 지각된 스트레스, 대인관계능력, 대학생활적응 간의 관계를 파악하고, 지각된 스트레스와 대학생활적응과의 관계에서 대인관계능력의 매개효과를 확인하기 위해 시도되었다. 연구참여자는 B시의 간호대학생 281명을 대상으로 하였다. 자료분석은 SPSS 23.0 프로그램을 이용하여 실수, 백분율, 평균, 표준편차, t-test, ANOVA, 피어슨 상관계수, 다중회귀분석, sobel test로 분석하였다. 대상자의 지각된 스트레스 평균 2.62±0.62점, 대인관계능력 평균 3.74±0.62점, 대학생활적응 평균 3.38±0.58점이었다. 대상자의 대학생활적응은 지각된 스트레스(r=-.478, p<.001)와 부적상관관계, 대인관계능력(r=.617, p<.001)과 정적상관관계를 나타냈다. 대상자의 지각된 스트레스와 대학생활적응과의 관계에서 대인관계능력이 부분매개효과(β=.629, p<.001)를 가지는 것으로 확인되었다. 따라서 간호대학생의 대학생활적응을 높이기 위하여 지각된 스트레스를 줄이고 대인관계능력을 높일 수 있는 교육 및 프로그램의 개발이 필요하다.

간호사의 다문화 효능감에 영향을 미치는 요인

양현주,변은경 사단법인 인문사회과학기술융합학회 2019 예술인문사회융합멀티미디어논문지 Vol.9 No.6

The purpose of this study was to explore degree of nurse's multicultural experience, multicultural perception, multicultural attitude, and multicultural efficacy, the factors influencing of multicultural efficacy of nurses and to provide the basic data to increased multicultural efficacy. This research involved 270 nurses who were working at 8 general hospital with at least 200 beds in B city and G do. Data were collected from July 15, 2015 to July 28. The analyzed by the ANOVA, Pearson's correlation coefficient, and multiple regression using SPSS/WIN 22.0. The degree of multiculture efficacy with the nurses was 50.51±6.72. There were not significant difference in multicultural efficacy with variable. There were positive correlation between multicultural efficacy and multicultural perception(r=.621, p<.001), between multicultural efficacy and multicultural attitude(r=.480, p<.001). Regression analysis showed 40.7% of variance in nurses multicultural efficacy can by experience of multicultural perception, multicultural attitude. These results indicated that it is necessary to determine the various factors affecting multicultural efficacy of nurses. And multicultural efficacy increasing education and program development are required. 본 연구는 간호사의 다문화 경험, 다문화 인식, 다문화 태도, 다문화 효능감의 정도를 알아보고, 다문화 효능감에 영향을 미치는 요인을 파악하여 다문화 효능감 증진을 위한 기초자료를 제공하기 위해 시행되었다. 본 연구는 B시와 G도에서 200병상 이상의 9개 종합병원에 근무하고 있는 간호사 270명을 대상으로 하였다. 자료수집은 2015년 7월 15일부터 2015년 7월 28일까지 였다. 수집된 자료는 SPSS 22.0 프로그램을 이용하여 실수, 백분율, 평균, 표준편차, t-test, 일원분산분석, 피어슨 상관계수, 다중회귀분석으로 분석하였다. 다문화 효능감 50.51±6.72점이었으며, 일반적 특성에 따른 다문화 효능감은 통계적으로 유의한 차이를 나타내지 않았다. 다문화 경험, 다문화 인식, 다문화 태도, 다문화 효능감과의 상관관계를 분석한 결과 다문화 인식(r=.621, p<.001), 다문화 태도(r=.480, p<.001)는 다문화 효능감과 상관관계를 나타냈다. 다문화 효능감에 영향을 미치는 변수는 다문화 인식(t=8.956, p<.001), 다문화 태도(t=3.158, p=.002)로 나타났고, 다문화 효능감을 40.7% 설명하는 것으로 나타났다. 본 연구 결과를 통해 간호사의 다문화 효능감에 영향을 미치는 요인을 확인하는 반복연구가 필요하다. 또한 다문화 효능감을 증진시킬 수 있는 교육 및 프로그램 개발이 요구된다.