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Park, Su Jung,Jung, Hyun Ju,Nguyen Dinh, Sy,Kang, Hunseung Informa UK (TaylorFrancis) 2016 RNA biology Vol.13 No.7
<P>Although seven proteins unique to U12 intron-specific minor spliceosomes, denoted as U11/U12-65K, -59K, -48K, -35K, -31K, -25K, and -20K, have been identified in humans and the roles of some of them have been demonstrated, the functional role of most of these proteins in plants is not understood. A recent study demonstrated that Arabidopsis U11/U12-65K is essential for U12 intron splicing and normal plant development. However, the structural features and sequence motifs important for 65 K binding to U12 snRNA and other spliceosomal proteins remain unclear. Here, we demonstrated by domain-deletion analysis that the C-terminal region of the 65 K protein bound specifically to the stem-loop III of U12 snRNA, whereas the N-terminal region of the 65 K protein was responsible for interacting with the 59 K protein. Analysis of the interactions between each snRNP protein using yeast two-hybrid analysis and in planta bimolecular fluorescence complementation and luciferase complementation imaging assays demonstrated that the core interactions among the 65 K, 59 K, and 48 K proteins were conserved between plants and animals, and multiple interactions were observed among the U11/U12-snRNP proteins. Taken together, these results reveal that U11/U12-65K is an indispensible component of the minor spliceosome complex by binding to both U11/U12-59K and U12 snRNA, and that multiple interactions among the U11/U12-snRNP proteins are necessary for minor spliceosome assembly.</P>
JY Park,AR Kim,SY Yoo,JI Kim,TW Jung,SH Woo,HY Heo,TW Kim,TS Ko 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
Grain sorghum is the fifth most important crop grown in the world for either a major food crop or animal feed. It is important to identify the genetic diversity of sorghum genetic resources for cultivar development and evaluation of sorghum accessions in Korea. Two hundred thirty six SSR primer sets, which are evely distributed across the sorghum genome, were used to assess the genetic variation of 23 sorghum accessions with a US cultivar, BTx623. Results showed that SSR markers were highly polymorphic among the sorghum collections and the average alleles per locus were 3.15 with the average of 0.436 PIC (polymorphism information content) values. The sorghum accessions in this study were unequally separated and were clustered into 4 groups. The results showed that there was a sufficient SSR polymorphism with SSR primers used among Korean sorghum accessions, and the development of genetic map and marker-assisted selection for cultivated sorghum would be feasible with further studies.
JY Park,AR Kim,SY Yoo,JI Kim,TW Jung,SH Woo,HY Heo,TW Kim,TS Ko 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
Excessive water stress can cause severe damage to sorghum and results in significant yield reduction. The aim of this study is to identify quantitative trait loci (QTL) for excessive water stress in sorghum. As a first step, two out of 21 bmr mutants were selected for their superior agronomic performance and Chlorophyll a fluorescence OJIP transient, and were crossed with an elite Korean cultivar, Hwangkeumchal, to construct mapping populations. One hundred ten out of 236 SSR primers showed polymorphism between two parens, which cover ten chromosomes of sorghum from different published SSR linkage maps of sorghum. Development of recombinant inbred lines from the crosses ‘25M2-0698 x Hwangkeumchal’ and ‘25M2-0404 x Hwangkeumchal’ are in progress using the single seed descendent method for generation acceleration.
Prioritized Multipath Video Forwarding in WSN
( Syed Muhammad Asad Zaidi ),( Jieun Jung ),( Byunghun Song ) 한국정보처리학회 2014 Journal of information processing systems Vol.10 No.2
The realization of Wireless Multimedia Sensor Networks (WMSNs) has been fostered by the availability of low cost and low power CMOS devices. However, the transmission of bulk video data requires adequate bandwidth, which cannot be promised by single path communication on an intrinsically low resourced sensor network. Moreover, the distortion or artifacts in the video data and the adherence to delay threshold adds to the challenge. In this paper, we propose a two stage Quality of Service (QoS) guaranteeing scheme called Prioritized Multipath WMSN (PMW) for transmitting H.264 encoded video. Multipath selection based on QoS metrics is done in the first stage, while the second stage further prioritizes the paths for sending H.264 encoded video frames on the best available path. PMW uses two composite metrics that are comprised of hop-count, path energy, BER, and end-to-end delay. A colorcoded assisted network maintenance and failure recovery scheme has also been proposed using (a) smart greedy mode, (b) walking back mode, and (c) path switchover. Moreover, feedback controlled adaptive video encoding can smartly tune the encoding parameters based on the perceived video quality. Computer simulation using OPNET validates that the proposed scheme significantly outperforms the conventional approaches on human eye perception and delay.