경도인지장애(Mild Cognitive Impairment)에서 단축형 Samsung Dementia Questionnaire(S-SDQ)의 임상적 유용성

윤지혜,김지혜,안인숙,정재원,송미선,김도관 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.5

Objuctives : Clinical utility of the Short Form of the Samsung Dementia Questionnaire (S-SDQ) as a screening instrument for Mild Cognitive Impairment (MCI). Methods : Three groups (n=27, Subjective Memory Impairment (SMI); n=41 MCI; n=79, Early Alzheimer's Di-sease(AD)) recruited from memory clinic were administered a battery of neuropsychological tests including S-SDQ and Korean version-Mini Mental State Examination (K.-MMSE). Results : S-SDQ, unlike the K-MMSE, had no association with the age and education ofthe subjects. Scores of S-SDQ was found to correlate (r=-.496) with scores of K-MMSE. Result of Receiver Operating Characteristic (ROC) analysis revealed that S-SDQ discriminated between the SMI and the MCI groups. Conclusion : S-SDQ may be a solution to the problem of contamination by education and age which affect traditional Cognitive screening instruments like K-MMSE, and it may be a useful tool for screening MCI.

기억력 저하를 호소하는 노인들을 대상으로 INM176를 3개월간 임상시험한 후의 치료성적 및 부작용 평가 보고

김지혜,고선규,고효정,권영아,김성환,김재경,김태은,박재우,서민영,송영란,이인수,김도관 大韓神經精神醫學會 2003 신경정신의학 Vol.42 No.2

Objectives : We examined the effects of INM 176 (K-l107) compared with placebo on the cognitive functions of 92 old aged subjects with cognitive impairment. Methods : This was a prospective, 12 week, double-blind, Placebo-controlled clinical trial. The elderly who achieved a score of less than 25 points on the K-MMSE or showed a high risk of Alzheimer's disease from the 7-Minute Neurocognitive Screening Battery were considered to have objective impairment and were selected as subjects for this study. The subjects were randomized to placebo or INM 176 group. The outcome measures were from the Alzheimer's Disease Assessment Scale-cognitive subscale (ADAS-Cog), the Instrumental Activities ofDaily Living (IADL) and the Korean Geriatric Depression Scale (KGDS) and two kinds of computerized priming tests. After setting the total error score in the Alzheimer's Disease Assessment Scale-Cognitive section (ADAS-cog) as the repeated measurement factor, an analysis of variance of the combined factor design was done between the placebo and INM 176 group. Results : The interaction effect of time (pre- and post-trial) and group (placebo and INM 176 group) was significant in the analysis of the ADAS-cog's total error score. The INM 176 group's total error score in the ADAS-cog decreased significantly (p<0.01), whereas the placebo group showed a slight increase. The mean changes in IADL and GDS from baseline scores favored in the INM 176 group than in the placebo group. Outcome changes ofADAS-cog, IADL, KGDS scores during the 12 week clinical trail ofINM 176 and placebo demonstrated favorable responses in the INM176 administered group. Conclusions : This is a preliminary clinical trial result of INM176 as a memory pill. Based on these results, INM176 may be a candidate molecule for the improvement of cognitive functions, including memory, Further clinical trial should demonstrate its efficacy.

데커시놀추출물의 경구투여후 흰쥐에 있어서의 약물속도론적 연구

김지혜,최송암,김동출 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.3

The objective of this study is to investigate the pharmacokinetic parameters of decursinol following oral administration in Sprague-Dawley rats. The plasm concentration of decursinol was determined by LC/MS with APCL positive mode. The m/z value of decursinol was observed at 247. Folowing oral administration of decursinol extract, the apparent clearance was 5.3±2.7 ml/hr/rat, the absorption half life was 2.5±0.41 hr, the elimination half life was 3.05±1.57 hr and the apparent volume of distribution was 21±12 ml/rat. The LC/MS method was successfully applied to the phar-macokinetic study of decursinol.

PCR과 SDS-PAGE를 이용한 Bifidobacteria의 동정

박시홍,송희성,이지연,김해영 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-

SDS-PAGE를 이용한 단백질 패턴의 분석결과, 같은 종의 Bifidobacterium은 동일한 단백질 패턴을 보이며, 다른 종의 Bifidobacterium은 서로 다른 패턴을 보이고 있는 것을 확인하였다. 따라서 SDS-PAGE를 이용한 whole cell protein 패턴 분석으로 Bifidobacterium을 간편하고 빠르게 동정할 수 있는 가능성을 보였다. DNA homology 98% 이상으로 PCR에 의해 동정에 어려움이 많았던 B. catenulatum, B. Pseudocatenulatum group, B. animalis을 SDS-PAGE로 확인한 결과, B. catenulatum과 B. pseudocatenulatum, B. animalis는 뚜렷한 단백질 패턴의 차이를 보임으로써 구별이 가능했다. PCR 수행 시 primer제작은 기존에 보고된 것과 NCBI blast program을 통하여 제작을 하였으며, 그 결과 B. longum, B. bifidum, B. adolescentis 3종류의 종에서만 특이적으로 band가 나타나는 것을 확인할 수가 있었다. 본 실험에서는 SDS-PAGE와 PCR을 이용한 균주 동정법으로 신속하고 정확하게 Bifidobacterium을 탐색할 수 있었으며, 발효유 제조나 의약품 제조 등 산업적으로 이용 될 수 있을 것이다 (Mitsuoka, 1984). Bifidobacterium is a Gram-positive obligate anaerobic bacterium which is able to change cellular shape by nutritional conditions and dominant species in intestinal microflora of human. In order to identify Bifidobacteria spp. in human intestine, PCR and SDS-PAGE of whole cell proteins were used. Species-specific primer sets based on the 16S rRNA, 16S-23S intergenic spacer and heat shock protein genes of three Bifidobacteria spp. were constructed using NCBI blast program (Bifidobacteria adolescentis, B. longum and B. bifidum). PCR products using each primer sets showed the same sizes of expected target genes. From the SDS-PAGE of whole cell proteins in bifidobacteria, profiles of SDS-PAGE between 13 type strains showed distinct differences. These methods will be useful for monitoring and evaluating Bifidobacteria spp. in human intestine microbial population.

Treacher Collins 증후군 환아의 증례보고

박지현,김승혜,송제선,김성오,이제호 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.3

Treacher Collins 증후군(TCS)은 25,000명에서 50,000명당 1명의 빈도로 비교적 드물게 발생하는 난치성 질환이다. 이 질환은 정상적인 부모에게서 돌연변이로 발생하거나 상염색체 우성으로 유전된다고 알려져 있다. 하악과 상악, 협골의 발육 부전, 부정교합, 양측성 이개 기형, 안검렬의 반몽고증 사면(antimongoloid slant)이 특징적이며, 종종 구순열, 구개열, 외이도 폐쇄, 중이와 내이의 기형에 의한 청각소실, 왜소증, 심장과 골격의 이상 등을 동반한다. 또한, 인두의 형성부전, 소구, 거설증, 악골의 기형에 기인하여 수면 중 무호흡증, 만성 폐쇄성 무호흡증 등의 호흡 장애가 있을 수 있으며, 전신마취 유도 시에 기관내 삽관이 어렵다고 보고된 바 있다. 치과의사는 관련 신드롬에 대한 충분한 이해를 통해 환자에게 보다 안전하게 치료를 제공해야 하며 구강 건강 관리 및 선천성 안면 기형에 대한 문제 해결을 돕기 위해 적절한 치료 및 안면 수술에 관련된 가이드를 제시해야 한다. 본 증례는 전반적인 치아우식을 주소로 연세대학교 치과병원 소아치과에 내원하여 외래에서 치아우식 치료를 받은 TCS 환아에 대한 것으로, 문헌 고찰을 통해 얻은 다소의 지견을 함께 보고하는 바이다. Treacher Collins syndrome(TCS) is a rare, incurable condition occurring in approximately 1 of 25,000 to 50,000 births. It may occur as a spontaneous mutation out of genetically normal parents or it may be manifested as an autosomal dominant pattern. TCS is characterized by facial deformities such as, underdevelopment of the maxilla, mandible and zygoma, malocclusion, bilateral deformities of auricles, antimongoloid slant of the palpebral fissures. The syndrome is often associated with cleft lip and palate, ear malformations and hearing loss, short stature, and anomalies of the heart and skeleton. Respiratory difficulty associated with air way obstruction may also be observed, and there is considerable difficulty in airway management during general anesthesia. It is necessary that dentists provide safe dental treatments and guidelines to TCS patients by providing adequate understanding about the characteristics of the syndrome and proper ways of managements. The purpose of this study is to report the dental and medical characteristics of the patient who visited the Department of Pediatric Dentistry, Yonsei University for multiple dental caries treatment and to review the literatures of TCS.

청소년의 스마트폰 중독과 우울 및 불안과의 관계

김경은,김필송,민주연,박수경,신서인,이지은,정해인,조호정,최정원,최정인 이화여자대학교 간호과학대학 2013 이화간호학회지 Vol.- No.47

Purpose: The purpose of this study was to investigate the relationships between the degree of smart phone addiction among adolescents and their depression and anxiety levels. Method: In this study, 379 high school students were selected using the convenient sampling method. The instruments used for this study were smart phone addiction measure, the CES-D (Center for Epidemiological Studies-Depression Scale), and the STAI (State-Trait Anxiety Inventory). Data were analyzed using the SPSS 20.0 program with descriptive statistics, t-test, ANOVA with Scheffe test, and Pearson’s correlation coefficient. Result: The mean score for smart phone addiction was 40.45±17.27, depression was 36.56±9.58, state anxiety was 44.00±9.61, and trait anxiety was 45.70±9.75. There were significant differences between the degree of smart phone addiction and the following variables: gender (t=-4.953, p<.001), hours of smart phone use per day (F=12.259, p<.001), types of frequently used features (F=3.485, p=.008), and satisfaction level for smart phone (F=5.18, p<.001). There were statistically significant relationships (p<.001) among degree of smart phone addiction, depression level, and state and trait anxiety levels. Conclusion: The results of this study suggested that there was a significant relationship between smart phone addiction and mental health, specifically, depression and anxiety among adolescents. Further research is needed to develop nursing strategies to provide care for adolescents who frequently utilize smart phones.

일부 자동차 공장 근로자들의 職業的 特性과 社會 心理的 Stress의 相關性 硏究

김성삼,홍지우,한현정,최희석,권소희,정해경,김삼태,송용선,이기남 한국전통의학연구소 2004 한국전통의학지 Vol.14 No.1

〈Objective〉 This study were carried out to study to assess the relationship between job characteristics, general characteristics, health behaviors, and psychosocial distress and suggested the preventive oriental medicine approaches in occupational health area. 〈Methods〉 We measured job characteristics(job demand, job control, jop insecurity) by The Karasek's Job Content Questionnaire, health behaviors(smoking, drinking, exercise, coffee), general characteristics at work by a self-administered questionnaire. Psycho social distress was measured by PWI(Psychosocial Well-being Index), a 18-item self- administered instrument. 370 data for research were collected through the process of oriental health examination with questionnaire in workplace. Statistical analysis was done by t test, oneway ANOVA, and hierarchical multiple regression analysis with SPSSWIN (version 10.0) 〈Results〉 Among the 152 respondents, the prevalence of high job strain was 27%(41 persons). The Odds ratio of high job strain was 3.11(95 2.12~4.11), and those of passive group and active group were 2.01(95 0.83~2.76) and 1.80(95 0.83~2.76), respectively. Our results supported the association between psychosocial distress and job strain. 〈Conclusion〉 The odds ratio of high strain isolated group(high strain group+low social support) was 3.59(95 2.05 5.13), but the odds ratio of high strain collective group was 1.32(95 0.23 2.41). Social support at work was modifing factor of the association between psycho social distress and job strain. Therefore we reviewed the evidence that economic depression was associated with psycho social distress in this study, and that the strength of the social support was important in maintain health.

일부 흡연 대학생의 지각된 스트레스와 흡연실태에 대한 상관관계 연구

강초록,권시내,권혜림,박수경,송인혜,오해인,이미지,한승아,황선아,김수지 이화여자대학교 간호과학대학 2003 이화간호학회지 Vol.- No.37

In spite of the seriousness of smoking harm has been developed these days. the increase of national smoking rate and that the people in the twenties are especially leading the rise of national smoking rate is an important issue of national health. Smoking of the university students can occur health problem and cause loss of manpower and expenses in their social activity period. So then the actual state for prevention of smoking and development of a program to prohibit smoking has to be seized urgently and starting with the study of the realities of university students, the effort to find a related fluent of smoking has to be studied. Related to this, the purpose of this study is to seize the general characteristics of smoking university students and to find out the relationship between perceived stress and smoking status among smoking university students. In addition, by collating the relationship between perceived stress and smoking status among smoking university students we have the purpose to furnish the basal data of stress care, the best way to cope with stress through group training and the plan for nursing implementation to decrease the smoking rate. For this study, a survey was conducted for 204 smoking university students of 13 universities in Seoul from 9th of May to 16th of May, 2003. The collected data was analyzed by t-test, Pearson Correlation and ANOVA in the statistical packages SPSS/PC. Major findings of this study are as follows; 1. Of the 204 respondents 85.4% of them were male and 14.6% of them were female. The average age of the sample was 23.19 years old and 41.8% of them were in senior, 21.4% in junior, 16.9% in sophomore, 19.9% in freshman grade. The major motivation to start smoking was 'because of curiosity'(33.8%) and the first smoking experience was at 'senior high school' (41.9%) and at 'college' (41.9%). 2 The highest scored stress factor of smoking university students were stresses about school work. 3. 72.7% had experience to quit smoking at least one or more times. Most of the reason to keep on smoking was 'short will to quit'(51.0%) and the smoking place was usually at 'school' (41.4%). Most respondents for the number ofsmoking friends were '5 people and above' (73.2%). 4. There was a negative relationship between the general characteristics and the entire perceived stress, however, there was a positive relationship between the general characteristics and the perceived stress in 4 categories which is 'sex distinction', 'major of study', 'residence form' and' inmate'. 5. There was a negative relationship between the general characteristics and the entire smoking status, however, there was a positive relationship between the general characteristics and the smoking status in 2 categories which is 'presence of hobby' and 'number of smoking friends'. 6. There was a negative relationship between the perceived stress and the smoking status. Through this study we have seized the relationship between the general characteristics, perceived stress and the smoking status of smoking university students. There was a positive relationship between some of the general characteristics with the perceived stress and the smoking status of smoking university students. Also there was a negative relationship between the perceived stress and the smoking status.

Chondrogenesis of Enhanced Green Fluorescent Protein-labelled Umbilical Cord Blood-derived Mesenchymal Stem Cells in Bioabsorbable Membrane

Ji-Hae Song,Yong-Soo Choi,Sang-Eun Noh,Jong-Il Kim,Bu-Young Park,Sang-Min Lim,Dong-Il Kim 한국생물공학회 2007 한국생물공학회 학술대회 Vol.2007 No.10

Changes of Stem Cell-specific M arkers of Umbilical Cord Blood-derived M esenchymal Stem Cells by Serial Passage

Ji-Hae Song,Yong-Soo Choi,Bu-Young Park,Jong-Il Kim,Hyung-Jin Jeon,Young-Joo Jeon,Mi-Eun Kong,Dong-Il Kim 한국생물공학회 2008 한국생물공학회 학술대회 Vol.2008 No.10