Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

작약의 RAPD 분석을 위한 PCR 최적조건 구명

최인수,김성만,김용철,이충렬,박현철 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

To optimize the PCR condition is one of the most important steps for RAPD analysis. The purpose of this study was to optimize PCR condition in Paeonia. 3×3×3 factorial experiment for template DNA concentration, MgCl2 concentration, and amount of taq polymerase was conducted. Another factorial experiment for reaction temperature(denature, annealing, and extension) was also conducted. The most appropriate template DNA concentration was 60ng. Clear bands were observed from 2.5mM and 4.5mM of MgCl2 if template DNA concentration and amount of taq polymerase were proper. Amount of taq polymerase for the optimal PCR condition was 0.5unit and 1unit. In the consideration of results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, 4 conditions (60ng of template DNA, 2.5mM MgCl2 and 0.5unit taq polymerase; 60ng of template DNA, 4.5mM MgCl2 and 0.5unit taq polymerase; 60ng of template DNA, 4.5mM MgCl2 and lunit taq polymerase; and 40ng of template DNA, 4.5mM MgCl2 and 1unit taq polymerase) were best combinations for the optimal PCR condition. Reaction temperature for the optimal PCR condition was 92℃, 36℃, 72℃.

미계측 유역의 부유물질 산정을 위한 다중회귀식 개발

최한규,박재용,박수진 강원대학교 산업기술연구소 2006 産業技術硏究 Vol.26 No.A

The purpose of this study is to present quantitatively the influence of variables that had the largest effect on the changes in suspended solids(SS), which would cause turbid water phenomenon, among water quality factors of the non-point pollution source, and then to develop a multiple regression equation of SS and predict the water quality of ungaged watersheds so as to provide basic data to establish efficient management plans for SS which flow in rivers and lakes. To identify the correlation of SS with the amount of rainfall and the state of land use, a simple correlation analysis and a simple regression analysis were conducted respectively. Finally, a multiple regression analysis was conducted to provide that SS were set as dependent variables while the amount of rainfall, paddy fields and dry fields were set as independent variables. As a result, the amount of rainfall had the most significant influence on changes in SS, followed by dry fields and paddy fields. In addition, the multiple regression equation was developed to predict SS in unmeasurable watersheds.

원자력 사고후 쌀알과 배추내 ^90Sr 및 ^137Cs 농도 예측

최용호,임광묵,황원태,이한수,이창우 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.3

원자력 사고후 시간경과에 따른 작물체내 핵종 농도를 보다 현실적으로 예측할 수 있는 방법을 수립하고 단위 건침적에 대하여 백미와 배추내 ^90Sr 및 ^137Cs의 50 년간 농도를 예측하였다. 비생육기 사고의 경우 농도는 두 핵종 모두 시간경과에 따라 서서히 감소하였고 50년 내내 ^90Sr이 ^137Cs보다 높았다. 생육기 사고의 경우 처음 1년간 농도는 비생육기 사고에 비해 대체로 ^90Sr은 최고 30 배, ^137Cs은 최고 1,000 배 정도까지 높았다. 50 년간의 누적농도는 백미에서는 비 생육기 사고의 경우 ^90Sr이, 생육기 사고의 경우 ^137Cs이 더 높았으나 배추에서는 어느 경우에나 ^90Sr이 더 높았다. 생육기 사고시 50년간의 누적농도에 대한 지배적 경로는 대체로 ^90Sr의 경우 뿌리흡수, ^137Cs의 경우 작물체 직접오염이었다. 재부유의 영향은 무시할 수 있을 정도였다. 예측 결과에 입각하여 사고 조건별로 대책의 방향을 제시하였다. A method of more realistically predicting radionuclide concentrations in crop plants varying with time after a nuclear accident was established to estimate 50 years' concentrations of ^90Sr and ^137Cs in polished rice seeds and Chinese cabbage for unit dry deposition. After non-growing season accidents, concentrations of both nuclides decreased gradually with time and ^90Sr concentrations were higher than those of ^137Cs throughout the whole period. Radionuclide concentrations in the 1st year after growing season accidents were on the whole higher than those after non-growing season accidents by factors of up to 30 for ^90Sr and up to 1,000 for ^137Cs. In polished rice seeds, the 50 years-integrated concentration was higher for ^90Sr than for ^137Cs after non-growing season accidents, whereas the opposite was true after growing season accidents. In Chinese cabbage, however, it was higher for ^90Sr than for ^137Cs after both types of the accident. Generally speaking, the dominant pathway for the integrated concentration after the growing season accident was root uptake for ^90Sr and direct plant contamination for ^137Cs. The effect of resuspension was negligible. Based on the predicted results, the direction of planning countermeasures was suggested for various accident conditions.

슬관절에 히야루론산 주입후 발생한 활막성 연골종증 : 증례 보고

최의성,김용민,김동수,손현철,박경진,조병기,배승환 충북대학교 의과대학 충북대학교 의학연구소 2009 忠北醫大學術誌 Vol.18 No.2

활막성 연골종증은 비교적 드문 양성 종양으로 관절내 활액막 결체조직의 양성 반응성 이형성에 의해 여러 개의 연골성 결절을 형성하고 이것이 관절내로 유리되어 유리체를 형성하는 질환으로 주로 슬관절에 발생한다. 활막성 연골종증의 정확한 발생 기전은 아직 밝혀지지 않았으나 외상, 감염 등이 원인으로 제시되고 있다. 저자들은 슬관절 내 히야루론산 주입후 발생한 슬관절내 활막성 연골종증을 1 례 경험하였으며, 관절경적 제거술을 통해 만족스런 결과를 얻었다. As a relatively rare benign tumor, synovial chondromatosis forms several cartilaginous nodules by the benign reactive metaplasia of synovial connective tissues within joints, which are loosed into the joints and cause a disease forming loose body mainly in knee joint. Accurate mechanism of synovial chondromatosis has not been clarified yet; however, trauma, infections and others have been suggested as its possible causes. The authors of this study experienced an example of a patient with synovial chondromatosis in knee joint occurred after the injection of hyaluronic acid in the joints and had a satisfactory outcome through the arthroscopic resection.

유기 능동 소자 제작을 위한 유기 박막의 제작 및 전기적 특성

崔淙宣,김대엽,李榕洙 弘益大學校 科學基術硏究所 1999 科學技術硏究論文集 Vol.10 No.2

There are currently considerable interest in the applications of conjugated polymers, oligomers, and small molecules for thin-film electronic devices. Organic materials have potential advantages to be utilized as semiconductors in field-effect transistors and light-emitting diodes. In this study, pentacene thin-film transistors (TFTs) were fabricated on glass substrate. Aluminums were used for gate electrodes. silicon dioxide was deposited as a gate insulator by PECVD and patterned by reactive ion etching (R.I.E). Gold was used for the electrodes of source and drain. The active semiconductor pentacene layer was thermally evaporated in vacuum at a pressure of about 10?? Torr and a deposition rate 0.3Å/s. The fabricated devices exhibited the field-effect mobility as large as 0.07㎠/V.s and on/off current ratio as larger than 10??.

MPLS망에서 RSVP와 DiffServ연동모델의 성능분석

최승권,오구영,지홍일,신승수,조용환 충북대학교 산업과학기술연구소 2002 산업과학기술연구 논문집 Vol.16 No.1

The explosive growth of the internet traffic has imposed tremendous stress on routers, particularly, in the core network. Today's IP services reflect the unpredictable and undifferentiated packet loss and jitter characteristics of traditional best-effort routers. For above requirements, it is required not only the high-speed transmission of the packet, but the new routing function, the guarantees of QoS and bandwidth, the supports of user-requested services MPLS is proposed by the IETF and many other research institute as the solutions for this problem. In this paper, analyze performance of QoS Model applied RSVP over DiffServ to MPLS network. this model is each RSVP applied access network and DiffServ applied core network. In Result, It is amiss that basis MPLS net uses to core network because the average packet loss rate and average delay time are increased if traffic increases. Experiment result was seldom difference in case of network that apply each RSVP, DiffServ, RSVP over DiffServ. RSVP over DiffServs case packet loss rate and delay time were low than DiffServ and the packet loss rate is high because comparing than RSVP but delay time was very low.

유자종실의 Sterol 조성

최상도,주옥수,조용운 진주산업대학교 농업기술연구소 1993 農業技術硏究所報 Vol.6 No.-

남해산 柚子種實油을 分析試料로 하여 日本, 基準油脂分析試驗法에 따라서 不檢化物量을 定量하고 T. L. C法으로 不檢化物을 分劃하였으며 G. C法에 依해 sterol組成을 分析하였다. 유자종실기름의 불검화물량은 기름 1g당 19.2mg으로서 1.9%이였고 불검화물의 분획 pattern은 less polar compounds가 20.3%, 4,4-dimethylsterol이 9.6%, 4-desmethylsterol이 47.1% 및 미전개물질이 22.9%이였다. sterol조성은 cholesterol이 0.3%, campesterol이 11.9%, stigmasterol이 9.3%, β-sitosterol이 72.2% 및 未知 sterol이 6.3% 이였다. 불검화물량, 불검화물의 분획 pattern 및 sterol조성은 他植物種實油와 거의 같은 경향을 나타냈다. This experiment was performed to the unsaponifiable matters content of the citrus seed oil. Also, the fractionated sterol pattern and the sterol composition of the citrus seed oil were examined. The citrus seed oil were contained 19.2mg unsaponifiable matters per gram. The unsaponifiable matters in seed oil were fractionated into less polar compounds(20.3%), 4, 4-dimethylsterol(9.6%), 4-monomethylsterol(0%), 4-desmethylsterol(47.1%) and undeveloped matters(22.9%). The sterol composition in citrus seed oil were cholesterol 0.3%, campesterol 11.9%, stigmasterol 9.3% and beta-sitosterol 72.2%. But brassicasterol and avenasterol were not detected.

대장균의 전사조절 유전자 nlp의 분자기구 해석

최용락,정수열,정영기,정정한 동의대학교 기초과학연구소 1994 基礎科學硏究論文集 Vol.4 No.1

An nlp (Ner like protein) gene from E. coli was previously cloned and sequenced. Here was show that expression of the sugar metabolism related genes, lacZ, malQ, and malP, increased 2.5- to 8.3-fold in the presence of a plasmid containing the nlp gene. This suggested that the nlp gene could induce maltose- and lactose-metabolism coordinately with crp*1 in the absence of cAMP. Using the nlp-lacZ fusion gene, it was possible to show the promoter of nlp was active in vivo. The overexpressed nlp gene product, a polypeptide of 10,000 daltons, was confirmed by SDS-polyacrylamide gel electrophoresis. The band shift assay revealed that the partially purified Nlp protein bound a specific DNA of the regulatory region of the nlp gene.

열간단조 후 공냉에 의해 제조된 내마모강의 마모특성

최병영,김익수,장병록,이용희 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.4

Wear of blades, buckets, plates and chisel occurred in operation of construction machinery, agricultural equipments, earthmoving equipments and offshore structures cause earlier failure of the components, resulting in the increased energy consumption, cost of maintenance and replacement. To solve the problems, advanced materials with higher resistance to abrasive wear have been attempted to make through alloy design and hot deformation followed by air cooling. 0.25C-1.4Mn-1.0Cr-0.7Ni-0.2Mo-0.08V-0.02Cu steels produced by hot forging were cooled in air to form bainite, showing higher resistance to wear than any other steels with different chemical composition in the sliding wear tests under the applied load of 74N. Higher strain hardening during sliding wear of 0.25C-1.4Mn-1.0Cr-0.7Ni-0.2Mo-0.08V-0.02Cu steels to cause microploughing wear mode seems to be one of the main reasons for showing higher resistance to wear than any other steels in this study.