Both the sera of patients with Behçet’s disease and <i>Streptococcus sanguis</i> stimulate membrane expression of hnRNP A2/B1 in endothelial cells

Cho, SB,Zheng, Z,Cho, S,Ahn, KJ,Choi, MJ,Kim, D-Y,Lee, KH,Bang, D Informa Healthcare 2013 Scandinavian journal of rheumatology Vol.42 No.3

<P><B>Objectives:</B> Heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 has been identified as a target antigen of anti-endothelial cell immunglobulin (Ig)A antibodies in patients with Behçet’s disease (BD). The aim was to investigate the effects of the sera from BD patients and <I>Streptococcus sanguis</I> on the subcellular expression of hnRNP A2/B1 in human dermal microvascular endothelial cells (HDMECs).</P><P><B>Method:</B> The sera of BD patients and healthy controls (HC) as well as cultured <I>S. sanguis</I> were used to stimulate HDMECs. Subcellular fractions were obtained from stimulated HDMECs and were subjected to immunoblot analyses. The distribution of hnRNP A2/B1 was investigated by immunocytochemistry and direct immunofluorescence study was performed in biopsy specimens of mucosal ulcers from BD patients.</P><P><B>Results:</B> BD patients’ sera increased the membrane expression of hnRNP A2/B1 in HDMECs after 12 and 24 h of incubation compared with HDMECs incubated with endothelial cell culture media and HC sera. <I>S. sanguis</I> also increased hnRNP A2/B1 in the cellular membrane. hnRNP A2/B1 mRNA level was also significantly upregulated in HDMECs incubated with BD patients’ sera and <I>S. sanguis</I>. Immunocytochemistry demonstrated marked expression of hnRNP A2/B1 in the cytoplasm and cellular membrane of HDMECs incubated with BD patients’ sera or <I>S. sanguis</I>. In addition, direct immunofluorescence experiments revealed the co-localization of serum IgA antibodies and monoclonal antibodies (mAbs) against hnRNP A2/B1 in tissue sections from ulcers of BD patients.</P><P><B>Conclusions:</B> Our data indicate that both the sera of BD patients with active disease and <I>S. sanguis</I> infection are inflammatory stimuli that can induce membranous hnRNP A2/B1 expression in HDMECs.</P>

임신율에 대한 성교후검사의 진단적 가치에 관한 연구

조경훈(KH Cho),이영준(YJ Lee),김정구(JK Kim),문신용(SY Moon),이진용(JY Lee),장윤석(YS Chang) 대한산부인과학회 1988 Obstetrics & Gynecology Science Vol.31 No.6

서울대학교 병원 산부인과 불임증 크리닉에 등록된 원인을 알 수없거나 단지 성교후 검사 결과 만이 좋지 않았던 109명의 환자에 대한 성교후 검사와 임신의 상호관계를 비교분석한 결과 다음과 같은 결론을 얻었다. 1. 자궁경관 점액검사를 받은 102명의 2년후 평균임신율은 32.4% (33/102)였고, 자궁경관점액 점수에 따른 임신율에는 차이가 없었다. 2. 자궁경관점액점수는 불임기간, 결혼기간 및 과거임신력과 관련이 없었으나 성교후 검사와 는 유의한 관계를 보여 주였다. 3. 양성성교후 검사군의 평균 연령이 음성성교후 검사군보다 더 낮게 나타났으나 불임기간 은 음성성교후 검사군에서 더 긴 것으로 나타났다. 4. 성교후 검사결과가 좋을수록 검사후 임신까지의 기간이 짧은 것으로 나타났다. 5. 2년간 추적후 도중에 추적 중단된 27명을 제외한 교정임신율은 양성성교후검사군이 41.4%(24/58), 음성성교후 검사군이 11.8%(2/17)로 양성검사군이 음성검사군보다 유의하게 높 은 것으로 나타났다. The postcoital test (PCT) has been a subject of controversy since it was first advocated by sims in 1866 . Opinions are still divided as to the value of the test in infertiliity investigations, especially in case of unexplained infertility. To evaluate relationship between the PCT result and the occurrence of pregnancy we analyzed the PCT results in 109 patients with unexplained infertility in relation to conception rates. The results obtained were summarized as follows; 1. The average conception rate 2 years after cervical mucus test (CMT) was 32.4 % (33/102) and there is no difference in conception rate as to the CMT score. 2. The CMT score didn`t show significant correlation with the duration of infertility and marriage, and the previous pregnancy performance but with the PCT result. 3. The positive PCT groups were younger than the negative groups , and the negateive groups showed longer duration of infertility than the positive groups. 4. The better the PCT result, the shorter the interval to conception. 5. The corrected conception rates 2 years after PCT were 41.4% (24/58) 11.8%(2/17) in positive and negateive groups respectively.

급성경막상 혈종의 보존적 치료

김강현,신문수,조기홍,김한규,조경기 대한신경외과학회 1988 Journal of Korean neurosurgical society Vol.17 No.4

Over a 5-year period the authers had treated conservatively 40 cases of the acute epidural hematomas, in which 33 lesions were in the supratentorial area and others in the infratentorial area. The configuration of the hematomas was mostly focal and most of them did not show mass effect in aspect of midline shift. But there were 2 cases with over 10㎜ of midline shift which were located at the frontoparietal and temporoparietal area. The hematomas at the parietal area and posterior fossa did not make the midline shift. 36 patients among 40 patients with the epidural hematomas were succussfully treated in the nonoperative management. The clinical findings of these patients were over 13 points of Glasgow coma scale, no focal neurological defictis and normal vital signs. And CT scan of these patients revealed less 10㎜ of midline shifting, less 40㏄ amount of hematoma, hematomas located in all area except temporal fossa and hematoma without associated intradural lesions. Four patients failed in conservative treatment underwent evacuation of the hematoma. Relative poor mentation on admission(G.C.S.≥13), large volume of hematoma, expansile volume course and associated intradural lesions were considered as failure factors.

부인과감염증에서 유나신과 세프라딘/겐타마이신의 치료효과에 대한 비교연구

조경훈(KH Cho),강순범(SB Kang),장윤석(YS Chang) 대한산부인과학회 1989 Obstetrics & Gynecology Science Vol.32 No.1

1988년 1월부터 7월까지 7개월간 서울대학교병원 산부인과에 부인 감염증으로 입원한 환자 에서 Unasyn투여군 20명과 대조군으로 Cephradine/Gentamicin투여군 20명을 비교검토하여 다음과 같은 결론은 얻었다. 1. 대상환자의 부인과 감염증으로는 Unasyn투여군과 Cephradine/Gentamicin투여군에서 골반염이 각각 12예, 13례로 가장 빈도가 높았다. 2. 세균배양에서 분리된 균주에 대한 항생제 예민반응검사에서 unasyn은 92.0%의 감수성을 Cephradine은 86.0%, Gentamicin은 60.0%의 감수성을 나타내었다. 3. 세균학적 판정효과는 unasyn군에서는 91.3%의 치유율을 Cephradine/Gentamicin군은 81.5%의 치유율을 나타내어 Unasyn군이 유의하게 우수함을 보였다. 4. 임상효과의 판정을 보면 Unasyn군은 95%의 치유율을 , Cephradine/Gentamicin군은 85%의 치유율을 보였다. 5. 종합판정에서도 Unasyn군이 Cephradine/Gentamicin군보다 유의하게 우수함을 나타내었다. 6. 투약중 양군에서 특이할만한 부작용은 나타나지 않았다. 7. 결론적으로 부인과 감염증에서 Unasyn단독투여는 Cephradine/Gentamicin병합 투여보다 우수한 항균효과를 나타내었다. To compare the efficacy of unasyn(sulbactam/ampicillin) in gynecologic infections with that of Cephradine plus Gentamicin a clinical study was done at the Department of Obsterics and Gyunecology , Seoul National Unviersity Hospital from January to July , 1988. Twenty patients were treated with Unasyn and the other twenty patients were treated with Cephradine plus Gentamicin . The results were as follows. 1. Pelvic inflammatory disease was the most frequency gynecologic infection in both study group . 2. Unasyn was sensitive to 92% of isolated organisms, Cephradine was to 86.0% and GM was to 60.0%. 3. In bacteriological response Unasyn group showed cure rate in 91.3% and Cephradine/Gentamicin group showed in 81.5%. 4. In clinical response Unasyn group was more cure rate in 95% and Cephradine/Gentamicin group revealed in 85%. 5. In global assessment Unasyn group was more superior to Cephradine/Gentamicin group . 6. no remarkable adverse effect was seen in Unasyn group and Cephradine/Gentamicin group 7. In conclusion Unasyn treatment was more effective than Cephradine/Gentamicin combination treatment in gyneclogic infections.

제왕절개술후 반흔과 충수돌기에 발생한 자궁내막증 2 예

최규홍(KH Choi),김희진(HJ Kim),이권해(KH Lee),조태호(TH Cho) 대한산부인과학회 1985 Obstetrics & Gynecology Science Vol.28 No.7

저자들은 1982년 2월 본 병원에서 한국인의 희귀한 제왕절개술 반흔에 발생한 자궁내막증과 1983년 4월 충수돌기에 발생한 자궁내막증을 각각 경험하였기에 문헌고찰과 함께 보고하는 바이다. We have experienced two cases of endometriosis of which one was found in abdominal wall scar formed after cesarean section and the other in the appendix during explorative laparotomy This paper presents those cases and a brief review of the literatures.

치골자궁저 높이 측정시 검사자간의 변동평가에 따른 3 가지 통게학적 방법의 비교

남계현 ( KH Nam ),이권해 ( KH Lee ),조태호 ( TH Cho ),이해혁 ( HH Lee ),선우재근 ( JG Sunwoo ) 대한산부인과학회 1991 Obstetrics & Gynecology Science Vol.34 No.11

1990년 3월 1일부터 3월 31일까지 순천향대학교 의과대학 산부인과 외래를 방문한 임신 20주부터 40주 사이에 무작위로 선택한 41명의 산모를 대상으로 검사자 3인이 치골자궁저 높이를 측정하여 다음과 같은 결과를 얻었다. 1. 검사자 A와 B간의 변동계수는 4.9%였다. 2. 검사자 A와 B간의 상관계수는 0.968였다. 3. 상기방법은 통계적으로 유의한 결과를 나타내지만, 동의한계란 방법으로 조사시 실제 동의한계는-4.3cm에서 2.6cm로 상당한 변동을 나타냈다. 4. 검사방법간의 차이와 제삼의 검사자를 이용하여 조사했을 때도 동의한게는 비슷한 결과를 나타냈다. 치골자궁저 높이의 백분율표를 이용하여 동의한계를 적용할 때 치골자궁저 높이의 측정은 실제 정상태아와 자궁내 태아발육지연아를 충분히 감별할 수 없다는 결론이 나왔으며, 자궁내 태아 발육지연아를 발견하는 선별검사로서, 치골자궁저 높이의 측정은 한계를 갖는다. 우리는 검사자간의 변동을 조사시, 상관계수나 변동계수를 이용하는 것보다 동의한계를 이용해 검사하는 것이 타당하다고 결론지었다. The measurement of symphysis-fundal height has been considered a simple and attractive screening method to detect the intrauterine growth retardation. Yet examination of published reports of fundal height measurement show wide variation in its performance. One possible reason for this wide variation is inter-observer variation. So, we assessed the variation by three statistical methods-the coefficient of variation, the correlation coefficient, and the limits of agreement. 1. The coefficient of variation between observer A & observer B was 4.9%. 2. The correlation coefficient between observer A & observer B was 0.968. 3. Above methods were highly significant statistically but, the limits of agreement were very wide and true limits of agreement were-4.3 to 2.6cm. 4. When the method of measurement was imperfect was tested, and when one of the observers was not good was tested, the results were similar to above results. Applying these limits to centile charts of symphysis-fundal height shows that the fundal height cannot be measured by different observers with sufficient agreement to seperate small fundal heights of those which are not small, and this severely limits the usefulness of measurement of the symphysis-fundal height as a screening test for intrauterine growth retardation. We conclude that inter-observer variation should be assessed by the method of limits of agreement, and not by calculating the coefficient of variation or the correlation coefficient.

복강내 대식세포가 난포 과랍세포의 황체홀몬과 난포홀몬 생성에 미치는 영향

송찬호,박기현,조동제,이병석,장경환,박원일,조환승 대한산부인과학회 1993 Obstetrics & Gynecology Science Vol.36 No.11

체외수정 및 자궁내 배아이식숙을 시행하는 불임 환자 10명에서 난자 채취시에 얻어지는 과립세포와 환자의 복강액에서 대식세포를 채취하여 이들을 병 뱅양하여 대식세포가 난포 과립세포의 홀몬 및 프로스타그란딘 생성에 미치는 영향을 조사하여 다음과 같은 결과를 얻었다. 1 . 황체홀몬농도는 hCG 투여군의 경우 배양 6일후에도 높게 유지되었으나 대식세포 병배양군과 대조군에서는 시간에 따라 감소하는 경향을 보였다. 배양 2일후 황체홀몬농도는 hCG투여군 및 대 식세포 병배양군에서의 농도가 대조군에 비해 유의하게 높았다. 4일후의 농도는 hCG투여군은 대식 세포 병배양군 그리고 대조군의 순이었으며 세군간에 유의한 차이를 보였다 6이후에는 hCG투여군 만이 다른 군에 비해 높은 농도를 나타냈다. 2. 난포홀몬 농도는 세군모두 배양 2일후에는 농도가 높게 유지되었으나 4일후 급격히 감소하여 그대로 지속되는 경향을 보였다. 난포홀몬 농도는 배양 2일 후에 대식세포 병뱅양군에서의 농도가 대조군에 비해 유의하게 낮았다. 4일후 및 6일후에는 세군간에 유의한 차이는 없었다. 3. PGE2농도는 세군 모두 배양 2일후에는 농도가 높게 유지되었으나 4일후 급격히 감소하여 그대 로 지속되는 경향을 보였다. PGE2농도는 다른 군에 비해 유의하게 높았다. 6일후에는 세 군간에 유의한 차이가 없었다. 이상의 결과로 보아 사람의 난포 과립세포은 체외배양시에도 홀몬 생성능력이 계속 유지되는 것을 확인하였으며 hCG첨가로 황체홀몬 생성이 유의하게 인하였으며 복강액내의 대식세포 난포 과립세 포의 황체홀몬 생성을 촉진하였으나 hCG보다는 효과가 약했으며 단기간에 그쳐 hCG와는 다른 기 전에 의해서 영향을 미치는 것으로 사료되는데 대식세포에서 생성되는 성정인자들에 의한 현상일 가능성을 생각할수 있다 난포 과립세포의 단독배양액에서도 프로스타그란딘이 측정됨으로써 난포 과립세포와 병배양 대식세포 등 두가지 세포에서 생성될 가능성이 있으리라 생각된다. To evaluate the action of macrophage on hormone production in human granulosa cell, we cultrues granulosa cell with and without maxrophage. The granulosa cells were obtained during oocyte retriveal in cases of IVF-ET. The peritoneal macrophages were obtained in cul-de-asc of the same patient .The granulosa cell were cultured alone(control group), with hCG, and with macrophage. The concentration of hormones were neasured in the media after 2,4 and 6 days. The production of progesterone was sustained after 6 days in hCG group. The progesterone production of granulosa cell co-cultured with macrophage was higher than control group up to 4 days, but was lower than hCGgroup. The estradiol production decreased abruptly 2 days after incubation in every group The concentration of estradiol was slightly lower in macrophage co-culture group after 2 days, and was not different form other group after 4 and 6days The prostaglandin e2 production decreaded 2 days after incubation in every group. The concentration of PGE2 was significantly higher in macrophage co-culture group after 2 or 4days, but this effexct did not continue after 6days. From this data, we can conclued that hCG has tropic effect on progesterone production of granulosa cell in vitro. Although macrophage showed similar tropic effect,the potency and duration were weaker and shorter than hCG. We think this hormone producing effect of macrophage was probably due to the activation of some growth factors.

골반벽을 침윤한 재발성 자궁경부암 환자에 대한 시술 1 예보고

김병기,박상윤,김종훈,박기복,이경희,이강현,이의돈,김미숙,이경화,황대용,조철구 대한산부인과학회 1995 Obstetrics & Gynecology Science Vol.38 No.11

최근 본원에서는 골반벽에 침윤된 국소 재발성 자궁경부암 환자를 대상으로 CORT를 시행하여 시술 후 6개월 현재 건강하게 살아 있기에 보고한다. 추가로 환자에서 CORT 에 대하여 설명할 때 대, 소변을 복부로 보게된다고 하면 치료를 포기하는 경향이 많은데 자궁암 말기의 비참한 죽음을 맞이하기보다 건강한 삶에 대한 희망을 갖고 새로운 삶을 영위하도록 권장되어져야 할 것으로 생각되며, 치료후에도 계속적으로 의료진과 가족들과 따뜻한 배려가 있어야 할 것으로 사료된다. The search for new treatment modalities for patients with recurrent gynecologic tumors infiltrating the pelvic wall appears to be of high priority in gynecologic oncology since the prognosis of these patients treated by conventional methods is very poor, although a significant number of patients might be cured if local control could be achieved. Recently, a combined operative and radiotherapeutic treatment(CORT) was developed for the treatment of pelvic wall recurrence based on considerations of tumor and radiation biology. We have experienced one case of CORT with good result. This case is presented with a brief review of the literatures.

신생쥐에서 뇌실질내 주입 Lipopolysaccharide에 대한 뇌의 염증반응

조규협,황성규,손윤경,강구성,김승래 대한신경외과학회 2003 Journal of Korean neurosurgical society Vol.33 No.5

비전형 만성골수성백혈병의 골수성 아세포기 1례

조희순,이채훈,김경동,이경희,배성화,조덕,양동욱 大韓血液學會 2003 大韓血液學會誌 Vol.38 No.3