Inhibitory Effects of Syk Transfection on Lung Cancer Cell Invasion

Peng, Chuan-Liang,Zhang, Ying,Sun, Qi-Feng,Zhao, Yun-Peng,Hao, Ying-Tao,Zhao, Xiao-Gang,Cong, Bo Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Objective: Spleen tyrosine kinase (Syk) is closely related to tumor invasion and metastasis, and has been shown to have potential inhibitory effects in tumors. In this study, we constructed a eukaryotic expression vector for Syk and analyzed its effects on invasive ability of the A549 non-small cell lung cancer cell line in vitro. Methods: A fragment of Syk was obtained by RT-PCR from human lung cancer cells and cloned into the expression vector pLNCXSyk. After restriction endonuclease digestion, PCR and DNA sequencing confirmation, the recombinant Syk expression plasmid was transfected into A549 human lung cancer cells using lipofectamine protocols. After selection, the cells stably expressed Syk. Detection of Syk expression of the cells by RT-PCR, and invasive ability were examined. Results: The eukaryotic expression plamid pLNCXSyk was constructed and expressed stably in the A549 human lung cancer cells. The RT-PCR results showed that Syk mRNA expression was upregulated significantly (P<0.05). Lower invasion through a basal membrane were apparent after transfection (P<0.05). Conclusions: A eukaryotic expression plasmid to cause Syk expression in lung cancer cells can obviously inhibit their invasive ability in vitro.

Case Reports : Paroxysmal Supraventricular Tachycardia in an Infant with Hand, Foot, and Mouth Disease

( Peng Hu ),( Shu Hou ),( Peng Fei Du ),( Jia Bin Li ),( Ying Ye ) 대한피부과학회 2012 Annals of Dermatology Vol.24 No.2

An 11-month-old male infant was admitted to our hospital with fever, fussiness, poor feeding, vomiting, and tachypnea for two days prior. Physical examination revealed sporadic papules and vesicles occurring on his hands, feet, face, and perianal mucosa. Enterovirus 71 was identified from both throat swab and vesicle fluid using virus isolation techniques. The patient`s heart rate fluctuated in a very narrow range from 180~210/beats/min regardless of his physiologic state. An electrocardiogram showed P-waves buried within or occurring just after regular, narrow, QRS complexes. The patient was diagnosed as having hand, foot, and mouth disease in combination with paroxysmal supraventricular tachycardia (PSVT). The child recovered well with symptomatic treatment, including intravenous administration of acyclovir, glucocorticoids, immunoglobulin, adenosine, and sotalol. PSVT was terminated within 36 hours of hospitalization. The skin lesions became crusted on the third day, and then proceeded to heal spontaneously. Here we report on this unusual case and review the associated literature. (Ann Dermatol 24(2) 200∼202, 2012)

Treatment of fever with traditional Chinese medicine according to Zheng on cancer patients (based on case reports)

Peng Cao,Lan-Ying Liu,Xue-Ting Cai,Xiao-Ning Wang,Jie-Ge Huo,Zhong-Ying Zhou 셀메드 세포교정의약학회 2012 셀메드 (CellMed) Vol.2 No.2

Fever in cancer patients is often due to the following causes: evil qi and toxity stagnancy, disorders of qi and blood, deficiencies of zang and fu organs, and the disorder of yin and yang. The treatments given to cancer patients with a fever are according to five: (a) Excessive inner heat and toxicants: remove heat and the toxicant, induce purgation. We use Cheng-Qi-Tang plus Qing-Wen-Bai-Du-Yin. (b) Tangle of damp and heat, and qi stagnancy: remove damp and heat, smooth the qi channel. We use Gan-Lu-Xiao-Du-Dan or San-Ren-Tang. (c) Obvious blood and heat stagnancy: remove heat and blood stasis. We use Xue-Fu-Zhu-Yu-Tang. (d) Deficiency of spleen qi, inner heat caused by a yin deficiency: nourish spleen qi and yin to remove the inner heat. We use Bu-Zhong-Yi-Qi-Tang or Xiao-Jian-Zhong-Tang. (e) Prominent yin deficiency and hectic fever: replenish yin and remove inner heat. We use Qing-Hao-Bie-Jia-Tang or Chai-Qian-Mei-Lian-San. The pathogenesis of fever in cancer patients is complicated. We can see both deficiency and excess in one differentiation. Therefore, we must make sure of it, then we can get the most effective treatment.

A genome-wide survey and analysis of basic helix-loop-helix genes in the Asian citrus psyllid, Diaphorina citri (Hemiptera: Psyllidae)

Ying Peng,Yong Wang,Xia-Fang Tao,Zhen Zeng,Nana Adwoa Nkuma Johnson,Qin Yao,Ke Ping Chen 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.3

The basic helix-loop-helix (bHLH) proteins are important player in regulation of various eukaryotic developmental processes such as neurogenesis, myogenesis and heart morphogenesis. The Asian citrus psyllid (Diaphorina citri) is a sap-sucking pest which is widely distributed in southern Asia and causes greening disease in citrus orchard. In this study, Diaphorina citri genome is found to encode 52 bHLH genes. Through in-depth phylogenetic analyses, all 52 Diaphorina citri bHLH (DcbHLH) genes are classified into correspondent bHLH families according to their orthology with bHLHs from fruit fly and other insect species. Examination of GenBank annotations of these 52 DcbHLH members indicated that annotations of only 21 DcbHLH proteins are consistent with our analysis. Therefore, our data provide useful information for updating annotations to other 31 DcbHLH proteins. Among the 52 DcbHLH proteins, DcClk, DcSima, DcCyc and DcCwo have dual HLH motifs, which have not been found in any other bHLH proteins. Diaphorina citri has 2, 3 and 2 bHLH members respectively in Net, Hand and SRC families while other insect species have only one member in these families. The existence of more Net, Hand and SRC family members probably means that Asian citrus psyllid possesses more complex regulatory network in controlling intervein emergence, heart morphogenesis and larval metamorphosis than other insects. These results provide a solid basis and interesting aspects for further investigations into the evolution and functions of bHLH genes in Diaphorina citri.

Schisandrin B Improves the Hypothermic Preservation of Celsior Solution in Human Umbilical Cord Mesenchymal Stem Cells

Zhang Ying,Wang Peng,Jin Mei-xian,Zhou Ying-qi,Ye Liang,Zhu Xiao-juan,Li Hui-fang,Zhou Ming,Li Yang,Li Shao,Liang Kang-yan,Wang Yi,Gao Yi,Pan Ming-xin,Zhou Shu-qin,Peng Qing 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.3

BACKGROUND: Human umbilical cord mesenchymal stem cells (hUCMSCs) have emerged as promising therapy for immune and inflammatory diseases. However, how to maintain the activity and unique properties during cold storage and transportation is one of the key factors affecting the therapeutic efficiency of hUCMSCs. Schisandrin B (SchB) has many functions in cell protection as a natural medicine. In this study, we investigated the protective effects of SchB on the hypothermic preservation of hUCMSCs. METHODS: hUCMSCs were isolated from Wharton’s jelly. Subsequently, hUCMSCs were exposed to cold storage (4 C) and 24-h re-warming. After that, cells viability, surface markers, immunomodulatory effects, reactive oxygen species (ROS), mitochondrial integrity, apoptosis-related and antioxidant proteins expression level were evaluated. RESULTS: SchB significantly alleviated the cells injury and maintained unique properties such as differentiation potential, level of surface markers and immunomodulatory effects of hUCMSCs. The protective effects of SchB on hUCMSCs after hypothermic storage seemed associated with its inhibition of apoptosis and the anti-oxidative stress effect mediated by nuclear factor erythroid 2–related factor 2 signaling. CONCLUSION: These results demonstrate SchB could be used as an agent for hypothermic preservation of hUCMSCs.

Influence of Personal Character on Quality of Life of Patients with Esophageal Cancer in North Henan Province and Influencing Factors

Lin, Jian-Ying,Wang, Ming-Sheng,Dong, Liang-Peng,Xu, Da-Wei,Wang, Ying,Li, Xue-Liang,Li, Xiu-Min Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

The aim of this study was to investigate QoL (quality of life) of patients with esophageal cancer in northern Henan province, China, and to accurate evaluate and reflect the relationship between patient characteristics and QoL. In the high risk area of esophageal cancer in the north of Henan province, 735 patients with esophageal cancer were investigated. The Eysenck personality questionnaire (EPQ) and QoL were analyzed by using the questionnaire of general situation, EPQ, QLQ-C30 and QLQ-OES18. The effects of personal character on the QoL of esophageal carcinoma patients were analyzed by SPSS 11.0 software. The QoL of esophageal cancer patients in Northern Henan region was significantly affected by character. The difference between choleric and type of melancholic temperament types was significant (P<0.01), also in OESEAT, OESTA, OESCO and OESSP (P<0.05). Differences in personal character can thus influence the quality of esophageal cancer patient lives.

Clinical Study on Safety and Efficacy of Microwave Ablation for Primary Hyperparathyroidism

Ying Wei,Lili Peng,Yan Li,Zhen-long Zhao,Ming-an Yu 대한영상의학회 2020 Korean Journal of Radiology Vol.21 No.5

Objective: To evaluate the safety, feasibility, and efficacy of microwave ablation (MWA) for the treatment of primary hyperparathyroidism (PHPT). Materials and Methods: This study enrolled 67 PHPT patients (22 men, 45 women; mean age, 56.0 ± 16.3 years; range, 18– 83 years) from January 2015 to December 2018. The laboratory data, including the serum intact parathyroid hormone (iPTH), calcium, phosphorus, and alkaline phosphatase (ALP) levels, were evaluated before MWA and again 2 hours, 1 day, 7 days, 1 month, 3 months, 6 months, 12 months, 18 months, and 24 months after. Results: Complete ablation was achieved with all 72 hyperplastic parathyroid glands found on the 67 patients enrolled, 64 of whom were treated in one session and 3 were treated over two sessions. The technical success rate was 100%. The median follow-up time was 13.6 months (range, 10.0–31.1 months). The clinical success rate was 89.4%. The volume reduction rate was 79.4% at 6 months. Compared to pre-MWA, the serum iPTH, calcium, phosphorus, and ALP levels had significantly improved 6 months post-MWA (iPTH, 157.3 pg/mL vs. 39.2 pg/mL; calcium, 2.75 ± 0.25 mmol/L vs. 2.34 ± 0.15 mmol/L; phosphorus, 0.86 ± 0.20 mmol/L vs. 1.12 ± 0.22 mmol/L; ALP, 79 U/L vs. 54 U/L, respectively; all, p < 0.01). Hoarseness was a major complication in 4 patients (6.0%), but it improved spontaneously within 2–3 months. Conclusion: MWA is safe, feasible, and effective for the treatment of PHPT.

Generation of Buffalo Mammary Epithelial Cells with Targeted Knockout of 18s rDNA by a CRISPR/Cas9 Adenovirus System

Peng Zhu,An-Qin Duan,Ting-Xian Deng,Xing-Rong Lu,Xiao-Ya Ma,Sha-Sha Liang,Chun-Ying Pang,Xian-Wei Liang 한국생물공학회 2017 Biotechnology and Bioprocess Engineering Vol.22 No.5

In recent years, CRISPR/Cas9 has rapidly become one of the most promising genome editing tools because it is simple and easy to use and cost effective. However, the large size of Cas9 sequences limits its application in clinically promising vectors and it also impacts non-viral transfection. In this study, CRISPR/Cas9 adenovirus vectors that target the buffalo 18s rDNA gene were constructed, transfected into 293 cells for adenovirus packaging, and the adenovirus was used to knockout the 18s rDNA gene in buffalo mammary epithelial cells. The results demonstrated that the CRISPR/Cas9 adenovirus vectors for the buffalo 18s rDNA gene could efficiently target the sites as revealed by the fluorescence reporter system. After amplification, the adenovirus titer of Sn458- 18s1 and Sn458-18s2 reached 1.03 × 109PFU/mL and 1.05 × 109 PFU/mL, respectively. For buffalo mammary epithelial cell infection, the efficiency was 100% when the multiplicity of infection (MOI) 100 PFU/mL. There were 9 mutational clones found in the 20 clones, and the gene mutagenesis rate reached 45%. Of these, 2 clones were 35-bp deleted and 7 clones were 12-bp deleted. These results suggested that the adenovirus system overcame the low transfection efficiency of the buffalo mammary epithelial cells associated with using lipid-based methods or electroporation. Moreover, we preliminary developed an efficient technique for multiple-locus gene targeting at repeated sequences of the buffalo genome.

An adaptive parameter optimization model and system for sustainable gear dry hobbing in batch production

Ying Zhang,Huajun Cao,Peng Chen,Li-Bin Zhu,Xiao Yang 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.6

Gear hobbing technology is one of the most widely used forming processes of gear teeth. And the development of dry hobbing technologyprovides a solution for realizing productive, economical, and ecological gear production. Since there is no cutting oil for coolingand lubrication in dry hobbing process, the hob tool life, thermal deformation errors of machine tool, and quality of workpiece are sensitiveto the cutting parameters, especially the cutting speed and tip chip thickness. Considering this situation, a dry hobbing parametersoptimization model with the hobbing efficiency as our objective, and the hobbing cost per piece, gear quality, tact time as constraints wasestablished, in which the cutting speed and tip chip thickness were considered as optimal variables and the material of workpiece, coatingof hob, and feed rate were considered comprehensively. An iterative test method is proposed to solve this model. And for the applicationin automated production line, an online adaptive application system was also developed based on SINUMERIK 840D NC system. Theparameters of five different kinds of material gear were optimized by applying this model and system, and the result showed the modeland the system were practical.

Research on General Model and Parameter Characteristics of Photovoltaic Array

Peng Zhang,Bi-ying Zhou 한국전기전자재료학회 2021 Transactions on Electrical and Electronic Material Vol.22 No.3

Under partial shading conditions, the P–U curve of PV (photovoltaic) array shows multiple local peaks. The traditional PV model cannot reflect this change. It is necessary to re-establish the mathematical model of the PV array suitable for complex lighting conditions. Based on the mathematical model of double diode PV cells, combined with the series–parallel theory of circuits, a detailed analysis of photovoltaic arrays under partial shading conditions is carried out, and the mathematical model of PV arrays under partial shading conditions is theoretically deduced by piecewise functions. The proposed general model is tested for irradiance intensity, temperature, and diode ideality factor and parallel resistance variable parameter sensitivity test in MATLAB. The output characteristics is tested and compared under uniform light and local shading conditions under random environment. The results show that the proposed model has good versatility and accuracy, and it can quickly and accurately reflect the I – U and P – U characteristics of PV arrays under complex lighting conditions under varying operating conditions, which provides strong support for related research on large-scale PV power generation systems.