Reformability evaluation of blasting-enhanced permeability in in situ leaching mining of low-permeability sandstone-type uranium deposits

Wang Wei,Liang Xuanyu,Niu Qinghe,Wang Qizhi,Zhuo Jinyi,Su Xuebin,Zhou Genmao,Zhao Lixin,Yuan Wei,Chang Jiangfang,Zheng Yongxiang,Pan Jienan,Wang Zhenzhi,Ji Zhongmin 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.8

It is essential to evaluate the blasting-enhanced permeability (BEP) feasibility of a low-permeability sandstone-type uranium deposit. In this work, the mineral composition, reservoir physical properties and rock mechanical properties of samples from sandstone-type uranium deposits were first measured. Then, the reformability evaluation method was established by the analytic hierarchy process-entropy weight method (AHP-EWM) and the fuzzy mathematics method. Finally, evaluation results were verified by the split Hopkinson Pressure Bar (SHPB) experiment and permeability test. Results show that medium sandstone, argillaceous sandstone and siltstone exhibit excellent reformability, followed by coarse sandstone and fine sandstone, while the reformability of sandy mudstone is poor and is not able to accept BEP reservoir stimulation. The permeability improvement and the distribution of damage fractures before and after the SHPB experiment confirm the correctness of evaluation results. This research provides a reformability evaluation method for the BEP of the low-permeability sandstone-type uranium deposit, which contributes to the selection of the appropriate regional and stratigraphic horizon of the BEP and the enhanced ISL of the low-permeability sandstone-type uranium deposit.

Multispectral Image Reproduction via Color Appearance Mapping

Ying Wang,Sheping Zhai,Zhongmin Wang 보안공학연구지원센터(IJSIP) 2014 International Journal of Signal Processing, Image Vol.7 No.4

One-step synthesis of Pt-Pd catalyst nanoparticles supported on few-layer graphene for methanol oxidation

Hongxia Wang,Leimei Sheng,Xinluo Zhaoa,Kang An,Zhongmin Ou,Yanghao Fang 한국물리학회 2018 Current Applied Physics Vol.18 No.8

In this study, Pt-Pd nanoparticles (NPs) supported on few-layer graphene (FLG) have been firstly prepared by one-step arc discharge evaporation of carbon electrodes containing both Pt and Pd elements. The few-layer graphene and Pt-Pd nanoparticles were achieved simultaneously through the evaporation process. After a hightemperature hydrogen treatment, the Pt-Pd/graphene was applied in the study of methanol oxidation in direct methanol fuel cell. The total weight of electrocatalyst keeps 2 wt% of the electrode. The sample with a mass ratio of Pt:Pd=3:1 (H-Pt3Pd1/G) exhibits better electrocatalytic activity (198 mA mg-1 pt) and better tolerance to carbon monoxide(CO) poisoning (If/Ib=1.26). It is noteworthy that the value of If/Ib can reach to 1.55 for the sample with the mass ratio of Pt:Pd=2:1 (H-Pt2Pd1/G),which implies its excellent ability of CO tolerance. The introduction of Pd element may open a new strategy to improve the CO tolerance by arc discharge evaporation.

Recombinant Human (rh)IL-4-Mediated Apoptosis and Recombinant Human IL-6-Mediated Protection of Recombinant Human Stem Cell Factor-Dependent Human Mast Cells Derived from Cord Blood Mononuclear Cell Progenitors

Oskeritzian, Carole A.,Wang, Zhiliang,Kochan, Janema P.,Grimes, Margaret,Du, Zhongmin,Chang, Hyeun-Wook,Grant, Steven,Schwartz, Lawrence B. 영남대학교 약품개발연구소 2000 영남대학교 약품개발연구소 연구업적집 Vol.10 No.-

Although stem cell factor(SCF) appears to be the major growth factor for human mast cells, other factors undoubtedly play important roles in the development, survival, and function of these cells. The current study examined the effects of recom-binant human(rh) IL-4 and rhIL-6 on rhSCF-dependent development and survival of human mast cells derived in vitro from cord blood progenitor cells. After 4-8 wk of culture with rhSCF and various amounts of rhIL-4, a dramatic decline in mast cell numbers was observed with rhIL-4, the EC_(50) being about 0.1ng/ml. Numbers of other cell types remained high. Mast cells derived from cord blood progenitors after 7 wk of culture with rhSCF alone displayed an MC_(T) phenotype and expressed Kit, FceRI, and IL-4R on their surface. Mast cells examined after purification by immunomagnetic sorting became apoptotic within hours after exposure to rhIL-4, a phenomenon blocked by anti-IL-4 Ab. Because rhIL-4-dependent apoptosis but not the loss of mitochondrial membrane potential potential was prevented by the pan-caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp-(Z-VAD)-fluoromethylketone, mitochondrial perturbation most likely preceded caspase activation. Consistent with this con-clusion was the observation that both apoptosis and loss of mitochondrial membrane potential(△Ψ_(m)) were inhibited by cyclosporin A in combination with aristolochic acid. rhIL-6 protected cord blood mast cells from rhIL-4-induced apoptosis. Thus, IL-4 can cause both maturation and apoptosis of human mast cells, the latter effect being abrogated by IL-6. The journal of Immunology, 1999, 163: 5105-5115.

The hydrogen storage nanomaterial MgH2 improves irradiation-induced male fertility impairment by suppressing oxidative stress

Jing Ma,Suhe Dong,Hongtao Lu,Zhongmin Chen,Huijie Yu,Xuejun Sun,Renjun Peng,Wei Li,Sinian Wang,Qisheng Jiang,Fengsheng Li,Li Ma 한국생체재료학회 2022 생체재료학회지 Vol.26 No.2

Objective: This study aimed to reveal the protective effect of hydrogen storage nanomaterial MgH2 on radiationinduced male fertility impairment. Methods: The characterization of MgH2 were analyzed by scanning electron microscopy (SEM) and particle size analyzer. The safety of MgH2 were evaluated in vivo and in vitro. The radioprotective effect of MgH2 on the reproductive system were analyzed in mice, including sperm quality, genetic effect, spermatogenesis, and hormone secretion. ESR, flow cytometry and western blotting assay were used to reveal the underlying mechanisms. Results: MgH2 had an irregular spherical morphology and a particle size of approximately 463.2 nm, and the content of Mg reached 71.46%. MgH2 was safe and nontoxic in mice and cells. After irradiation, MgH2 treatment significantly protected testicular structure, increased sperm density, improved sperm motility, reduced deformity rates, and reduced the genetic toxicity. Particularly, the sperm motility were consistent with those in MH mice and human semen samples. Furthermore, MgH2 treatment could maintain hormone secretion and testicular spermatogenesis, especially the generation of Sertoli cells, spermatogonia and round sperm cells. In vitro, MgH2 eliminated the [·OH], suppressed the irradiation-induced increase in ROS production, and effectively alleviated the increase in MDA contents. Moreover, MgH2 significantly ameliorated apoptosis in testes and cells and reversed the G2/M phase cell cycle arrest induced by irradiation. In addition, MgH2 inhibited the activation of radiation-induced inflammation and pyroptosis. Conclusion: MgH2 improved irradiation-induced male fertility impairment by eliminating hydroxyl free radicals.

Core fucosylation of maternal milk N-glycan prevents intestinal inflammation by regulating Treg/Th17 balance in infant gut microbiota

Tao Jia,Ran Yan,Pan Zhongmin,Wang Li 대한독성 유전단백체 학회 2022 Molecular & cellular toxicology Vol.18 No.1

Background This study elucidated the regulatory roles of milk glycoprotein core fucosylation on the gut microbiota of breast-fed infants. Objectives In order to investigate the effects of core fucosylation of maternal milk N-glycan on the intestinal functions in newborn mice, Fut8 +/+ → Fut8 +/+ and Fut8 ± → Fut8 +/+ models were established. Results The results indicated that the gut microbiota diversity was altered and production of Bifidobacterium and Lactobacillus was reduced in the intestine of Fut8 ± → Fut8 +/+ mice . Furthermore, the levels of SCFAs were decreased in the gut of Fut8 ± → Fut8 +/+ infant. To further investigate the effects of core fucosylation of breast milk N-glycan on the intestinal inflammation in newborns, NEC models were established, and Fut8 +/+ off -springs with experimentally induced NEC were fed with the breast milk of Fut8 +/+ and Fut8 ± maternal mice. Our data revealed that reduced core fucosylation of breast milk N-glycan could be responsible for the intestinal inflammation in NEC off spring, as the production of pro-inflammatory cytokines including IL-1b, IL-6, IL-17A and TNF-a was significantly upregulated in Fut8 ± → Fut8 +/+ NEC group, while the levels of anti-inflammatory cytokine IL-10 were remarkably reduced. Furthermore, Treg/Th17 balance in the intestine was disturbed in Fut8 ± → Fut8 +/+ NEC newborn, suggesting the impaired immune development caused by reduced core fucosylation of breast milk. Conclusion These fi ndings indicated that core fucosylation of maternal milk N-glycan could assist the colonization of gut Lactobacillus and Bifidobacterium and regulate the Treg/Th17 balance, subsequently preventing intestinal inflammation in breast-fed newborns.

A novel scheme to acquire enhanced up-conversion emissions of Ho3+ and Yb3+ co-doped Sc2O3

Chaoyi Zhang,Qian Jiang,Xinyu Wang,Jing Liu,Yantao Xiao,Chun Li,Hai Lin,Fanming Zeng,Zhongmin Su 한국물리학회 2020 Current Applied Physics Vol.20 No.1

A detailed investigation about the effect of Sc2O3: 1 mol%Ho3+/5 mol%Yb3+ co-doped with Ce4+ ions prepared by sol-gel methods was performed systematically. Under the excitation of 980 nm laser diode, both green emission (553 nm, 5F4/5S2→5I8) and red emission (672 nm, 5F5→5I8) were both observed in the emission spectra of the samples, which were found to be two-photon process and sensitized by Yb3+ ions. With the increasing of Ce4+ ions, the up-conversion green emission intensity are increased by 6.52, 8.69, 10.85, 13.92 and 16.66 fold, corresponding to the Ce4+ ions concentrations from 5 mol% to 13 mol%, respectively. The number of photons are necessary to populate the upper emitting state decreases to 2 and the infrared absorption coefficient is reduced, when the Ce4+ ions concentration increase to 13 mol%. Ce4+ ions play an important role in tailoring the local crystal field around Ho3+ ions, lowering the highest phonon cut-off energy of matrix and reducing the infrared absorption coefficient, thus hindering the non-radiative processes, which contribute to the increased emission intensity. The excellent enhancement makes it a promising multifunctional optical luminescence material.

New Insights Into Refractory Chronic Cough and Unexplained Chronic Cough: A 6-Year Ambispective Cohort Study

Zhang Mengru,Morice Alyn H.,Si Fengli,Zhang Li,Chen Qiang,Wang Shengyuan,Zhu Yiqing,Xu Xianghuai,Yu Li,Qiu Zhongmin 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.6

Purpose: Only limited studies have depicted the unique features and management of refractory chronic cough (RCC) and unexplained chronic cough (UCC). These led to the initiation of this study, which reported the demographic characteristics, manifestations, and long-term outcomes on a large series of consecutive RCC/UCC patients, providing a guideline-led real-world clinical experience. Methods: Retrospective baseline information was obtained from Clinical Research Database (January 2016 to May 2021). At least 6 months after the last clinic visit, included subjects were prospectively followed up. Results: Three hundred and sixty-nine RCC and UCC patients (199 females, 53.9%) were analyzed. The median cough duration was 24.0 (12.0–72.0) months. Laryngeal symptoms were reported in 95.9% of the patients. The common triggers for coughing were talking (74.9%), pungent odors (47.3%), eating (45.5%), and cold air (42.8%). RCC was considered in 38.2%, and the remainder of 228 patients had UCC, with an equal sex distribution (P = 0.66). Among the 141 RCCs, 90.8% (128) had refractory reflux cough, which was more responsive to current treatments (P < 0.01). Although most features and test results between RCC and UCC were similar, UCC was more commonly inappropriately treated (P < 0.01). Nineteen (7.7–41.1) months after the final clinic visit, 31.2% still coughed persistently, while 68.8% reported cough improvement or remission. RCC reported more favorable treatment outcomes (including cough improvement, control, and spontaneous remission) than UCC (P < 0.01). Coughs with long duration before the initial cough clinic visit (P < 0.01), frequent urinary incontinence (P < 0.01), and being sensitive to “talking” (P < 0.01) or “cold air” (P < 0.01) were less likely to be solved. Conclusions: The current treatments only improve cough symptoms in two-thirds of patients. Clinical indicators for treatment failure were those coughing for long duration and being sensitive to “talking” or “cold air.”

Effective transportation of electrons/Li ions in V2O3 nanoparticle/carboncoated stainless steel composite electrodes for lithium-ion storage

Yaxiong Tian,Jianghua Zhang,Tunmise Ayode Otitoju,Honggang Wang,Huaxia Chen,Lin Zhu,Zhongmin Feng,Ting Sun 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.114 No.-

The performance of V2O3 is threatened as a result of relatively sluggish electrons/ion transportation andstructural pulverization in lithium-ion batteries. The rational design of the electrode structure to exhibitgreat lithium storage of V2O3 is crucial. In this work, we report stable composite electrodes by integratingV2O3 nanoparticles with an uneven carbon-coated stainless-steel oxide (V2O3/C/SS) current collector viaan in-situ growth method. They allow fast electrical conductivity through the carbon coating and reductionstrategy. Meanwhile, the small nanoparticle size of V2O3 on V2O3/C/SS as well as the undestroyed 3DV–V skeleton are helpful to Li-ion diffusion. The weak interaction between Li ions and V2O3 achieves fastand high Li storage behavior, which proves by electrochemical kinetics analysis and DFT calculation. Theelectrode delivers high rate capacitive and long cycling stability with 564 mAh g1 and 310 mAh g1 at100 mA g1 and 1 A g1, respectively, and a specific capacity of 118 mAh g1 is achieved after 1000 cyclesat 5 A g1. The excellent electrochemical performance may be ascribed to the unique design for effectiveelectron and Li-ion transportation. This work offers a new prospect for the development of highperformancebinder-free V2O3-based electrodes.

Clusterin negatively modulates mechanical stress-mediated ligamentum flavum hypertrophy through TGF-β1 signaling

Liu Chunlei,Li Peng,Ao Xiang,Lian Zhengnan,Liu Jie,Li Chenglong,Huang Minjun,Wang Liang,Zhang Zhongmin 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Ligamentum flavum hypertrophy (LFH) is a major cause of lumbar spinal canal stenosis (LSCS). The pathomechanisms for LFH have not been fully elucidated. Isobaric tags for relative and absolute quantitation (iTRAQ) technology, proteomics assessments of human ligamentum flavum (LF), and successive assays were performed to explore the effect of clusterin (CLU) upregulation on LFH pathogenesis. LFH samples exhibited higher cell positive rates of the CLU, TGF-β1, α-SMA, ALK5 and p-SMAD3 proteins than non-LFH samples. Mechanical stress and TGF-β1 initiated CLU expression in LF cells. Notably, CLU inhibited the expression of mechanical stress-stimulated and TGF-β1-stimulated COL1A2 and α-SMA. Mechanistic studies showed that CLU inhibited mechanical stress-stimulated and TGF-β1-induced SMAD3 activities through suppression of the phosphorylation of SMAD3 and by inhibiting its nuclear translocation by competitively binding to ALK5. PRKD3 stabilized CLU protein by inhibiting lysosomal distribution and degradation of CLU. CLU attenuated mechanical stress-induced LFH in vivo. In summary, the findings showed that CLU attenuates mechanical stress-induced LFH by modulating the TGF-β1 pathways in vitro and in vivo. These findings imply that CLU is induced by mechanical stress and TGF-β1 and inhibits LF fibrotic responses via negative feedback regulation of the TGF-β1 pathway. These findings indicate that CLU is a potential treatment target for LFH.