가공조건에 따른 녹채 주스의 색도 변화

윤영빈,최언호 서울여자대학교 자연과학연구소 2003 자연과학연구논문집 Vol.15 No.-

녹채 주스의 열처리와 원심분리, 이에 갈변방지제와 다른 과채류의 첨가가 주스의 색도 안정성에 미치는 영향을 조사하였다. 녹색 채소인 셀러리와 돌미나리 착즙액을 2: 1 로 혼합하여 착즙전 (100℃, 30초) 과 착즙후 (96 ℃, 15초) 의 열처리와 원심력 (500, 1,000, 5,000, 10,000 xg) 에 따른 색의 변화를 조사한 결과 -a값(녹색도) 과 a/b 값이 열처리와 원심력이 증가함에 따라 급격히 감소하였다. 원심분리는 착즙액 자체의 녹색도를 저하시켰으나 그 후 색의 안정에 효과적이었다. 셀러리-돌미나리 착즙 혼합액에 사과, 당근, 대추-구기자를 각각 흔합한 후 5℃와 25℃에서 저장한 결과 25℃에서 당근과의 혼합에 의한 녹색의 저하가 가장 큰 것으로 나타났다. Cysteine과 glutathione의 첨가는 주스의 색도변화에 영향을 미치지 못하였다. The effects of processing conditions such as thermal treatment, centrifuge, addition of anti-browning agents, and mixing with non-green vegetable and fruit extracts on change in colors of green vegetable juices during storage were investigated. The mixture of celery and watercress extracts at the ratio of 2 : 1 was heated at 96℃ for 15 sec and centrifuged at levels of 500, 1,000, 5,000 and 10,000xg. The Hunter value of -a (greenness) decreased by thermal treatment and with an increase in g values of centrifuge. Addition of the liquid extracts from fruits and vegetables such as apple, carrot, jujube, and lycii to celery-watercress juice decreased the -a value of the juice due to the reduction of pH with storage time, resulting in an increase of Hunter value of b. The trend was more remarkable with the addition of carrot. Therefore, considering change in color due to the unstability of chlorophyll of green vegetables, it is expected to reduce decoloration during storage by the adjustment of the mixing ratio of raw materials and centrifugal force. Cysteine and glutathione did not influence on color stability of green vegetable juice.

혼합과채주스의 품질특성에 미치는 열처리와 한외여과의 효과

윤영빈,최언호 서울여자대학교 자연과학연구소 2003 자연과학연구논문집 Vol.15 No.-

사과, 당근, 샐러리, 돌미나리, 대추, 구기자의 착즙액을 3 : 3 : 1 : 1/2 : 1 : 1/2 의 비율로 혼합한 과채주스를 열처리 (96 ℃에서 15초) 또는 한외여과 (MW cut off value 80,000) 하여 밀봉하고 5℃ 와 25℃ 에서 8주 동안 저장하고 저장특성을 조사하였다. pH는 무처리구와 열처리구의 경우 4.83-4.85, 한외여과구의 경우 5.85로 나타났으며 환원당, 유리당, 유기산 등은 한외여과구에서 낮은 값을 보였다. 저장기간이 증가함에 따라 무처리구의 성분 변화가 심하게 나타났다. 총균수는 저장초기 무처리구에서 5.6 x10¹로 나타났으며 저장기간이 증가함에 따라 급격히 증가되었으며 무처리구 이외의 실험구에서는 미생물이 전혀 검출되지 않았다. 저장기간에 따른 흔합과채쥬스의 품질특성은 5℃ 에서 8주 저장하는 동안 무처리구에서 변화가 가장 심하여 pH 저하, 환원당의 감소 lactic acid의 증가, L-ascorbic acid 의 감소, 효소활성에 의한 색도의 변화 등을 야기하는 결과를 초래하였으며 25℃ 저장구에서는 그 변화가 더 심한 것으로 나타났다. 열처리구와 한외여과구에서는 대체적으로 성분에 큰 변화가 없었으며 특히 한외여과구에서 더 안정한 것으로 나타나 한외여과 처리와 저온 저장에 의하여 저장수명을 더 연장시킬 수 있을 것으로 기대되었다. Mixed fruit and vegetable juices which were composed of apple, carrot, celery, watercress, jujube and lycii at the weight ratio of 3 : 3 : 1 : 1/2 : 1 : 1/2 were heated for 15sec at 96°Cor filtered through a ultramembrane filIer(MW cutoff value 30,000) and stored for 8 weeks at 5° and 25℃. The initial pH was 4.83-4.85 in the control and the heat-treated juices and 5. 85 in the ultrafiltered juices. The ultrafiltered juices showed lower contents of reducing sugars, free sugars and organic acids than those of the control and the heat-treated juices before storage. During storage the control juices showed decrease in pH, reducing sugars and L-ascorbic acid, an increase in lactic acid and the change in color by enzyme action, more severely at 25℃, while the heat-treated juices and the ultrafiltered juices did not show remarkable changes in their composition. The storage life of juices was able to be prolonged by thermal treatment and ultrafiltration, when followed by low temperature storage.

Benzoate 분해균주의 분리 및 특성

윤영빈,정원철,최언호 서울여자대학교 자연과학연구소 1997 자연과학연구논문집 Vol.9 No.-

Twenty-seven bacterial colonies capable of growing on benzoate as the sole source of carbon were isolated from streamside soils. Three of isolates were found to be extremely effective in metabolizing the benzoate, when they were incubated in the basal salt medium containing 2,000ppm of benzoic acid at 30℃ for 48hrs. The three isolates, Y205, W212 and J211 were identified Pseudomonas aeruginosa, Bacillus fastidiosus, and Aeromonas sp. respectively through morphological and physiological examination. Optimal temperature and pH for benzoate degradation of the isolates appeared to be 35℃ and pH for Pseudomonas sp. Y205 and Bacillus. sp. W212, and 30℃ and pH 7.0 for Aeromonas sp. J211. The highest degradation of benzoate by the three isolates was obstained in the growth media containing ammonium sulfate as nitrogen source. Optimal concentration of ammonium sulfate was 6.0g/l for Pseudomonas sp. Y205 and Aeromonas sp. J211, and 4.0g/l for Bacillus. sp. W212. When the three isolates were cultivated in basal salt medium containing 2,000ppm benzoate under optimal condition, they showed a logarithmic phase growth 12hours and a stationary phase growth 18hours after innoculation, and the benzoate degradation of 91.15-92.40% after 72-hours incubation. Benzoate degradation increased in parallel with the cell growth.

BioSMACK: a Linux Live CD for genome-wide association analyses

( Chang Bum Hong ),( Young Jin Kim ),( Sang Hoon Moon ),( Young Ah Shin ),( Min Jin Go ),( Dong Joon Kim ),( Jong Young Lee ),( Yoon Shin Cho ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.1

Recent advances in high-throughput genotyping technologies have enabled us to conduct a genome-wide association study (GWAS) on a large cohort. However, analyzing millions of single nucleotide polymorphisms (SNPs) is still a difficult task for researchers conducting a GWAS. Several difficulties such as compatibilities and dependencies are often encountered by researchers using analytical tools, during the installation of software. This is a huge obstacle to any research institute without computing facilities and specialists. Therefore, a proper research environment is an urgent need for researchers working on GWAS. We developed BioSMACK to provide a research environment for GWAS that requires no configuration and is easy to use. BioSMACK is based on the Ubuntu Live CD that offers a complete Linux-based operating system environment without installation. Moreover, we provide users with a GWAS manual consisting of a series of guidelines for GWAS and useful examples. BioSMACK is freely available at http://ksnp.cdc. go.kr/biosmack. [BMB reports 2012; 45(1): 44-46]

심한 골병변으로 발현된 기능성 낭종성 부갑상선 선종

전숙,김영희,박지영,고관표,박철영,김덕윤,우정택,김성운,김진우,김영설,고석환 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.2

낭종성 부갑상선 선종과 심한 골병변을 동반한 부갑상선 기능항진증은 매우 드문 질환으로서, 저자들은 양측 고관절의 통증을 초기 주소로 내원한 환자에서 고칼슘혈증과 부갑상선 호르몬 증가, 골병변의 방사선적 소견을 통해 부갑상선 기능항진증을 진단하고, 경부 초음파와 컴퓨터 단층 촬영, 부갑상선 스캔검사 및 수술중 부갑상선 낭종액 검사 등을 통해 기능성 부갑상선 낭종의 한 종류인 낭종성 부갑상선 선종을 진단하고 수술적 제거를 통하여 정상화된 1예를 경험하였다. A cystic parathyroid adenoma is rare. A case of primary hyperparathyroidism, with the cystic formation of a parathyroid adenoma and a severe bony lesion, is reported. A 52-year-old male was admitted due to pain in both hips and for evaluation of hypercalcemia. The plasma level of the intact parathyroid hormone (iPTH) was elevated to 1424 pg/mL. Ultrasonography and the computed tomography revealed a parathyroid cyst on the left thyroid lower pole. Parathyroid scintigraphy detected a parathyroid adenoma. A radiograph showed a subperiosteal bone resorption on the phalanges, and a brown tumor (osteitis fibrosa cystica) on the femur shaft was noted. A surgical excision of the parathyroid adenoma was performed. The PTH level in the cystic fluid was increased. A histological examination confirmed a cystic parathyroid adenoma. The PTH level was normalized after the operation (J Kor SOC Endocrinol 18:214-220, 2003).

3D 광학 스캐너를 이용한 인체모형 팬텀 표면 데이터 획득 및 X-ray 에너지의 몬테카를로 시뮬레이션(MCs)을 이용한 팬텀 선량 평가와 영상구현

신준봉(Jun-Bong Shin),이도희(Do-Heui Lee),윤경준(Kyoung-Jun Yoon),박민재(Min-Jae Park),고영문(Young-Moon Go),김성우(Sung-Woo Kim),홍승모(Seung-Mo Hong),이현철(Hyun-Cheol Lee),이의섭(Ui-Seob Lee),노선영(Seon-Yeong No),강석윤(Seok-Yoon 대한영상의학기술학회 2021 대한영상의학기술학회 논문지 Vol.2021 No.-

목 적:본 연구의 목적은 기존 컴퓨터단층촬영(Computed tomography, 이하 CT) 스캔 방법 대신 3D 광학 스캐너 (3D optical scanner)를 이용하여 인체모형 팬텀의 표면 데이터를 획득하고 기존 CT 스캔 표면 데이터와 3D 광학 스캔 표면 데이터 차이를 비교 분석하는 것이며, 3D 광학 스캔 표면 데이터를 몬테카를로 시뮬레이션(Monte Carlo Simulation, MCs)에 적용하여 일반 촬영 에너지조건으로 팬텀 선량 평가와 영상 구현을 연구하는 것입니다. 연구방법: 일반촬영의 선량 평가를 위해 환자 표면 데이터를 얻을 때 3D 광학 스캐너를 활용할 것을 제안합니다. 우리는 3D 광학 스캐너(Go!SCAN SPARKTM, CREAFORM) 사용하여 기존 CT 스캔 방법을 대신해 인체모형 팬텀의 표면 데이터를 얻었습니다. 3D 스캔 표면 데이터를 정렬하고 편집하기 위해 Meshlab 프로그램을 이용하였으며, 3D slicer 프로그램을 이용하여 CT 스캔 표면 데이터와 3D 스캔 표면 데이터 차이를 정량적으로 비교분석 하였습니다. 또한 3D 스캔 표면 데이터 분석 프로그램(VXelements, CREAFORM)을 사용하여 인체 모형 팬텀의 재현성을 평가 하였습니다. SpekCalc 프로그램을 이용하여 X-ray beam 에너지 스펙트럼을 계산하고 Geant4 기반의 MCs인 TOPAS를 이용하여 X-ray Beam을 모델링 하였습니다. 인체모형 팬텀을 MCs에 적용 하여 일반촬영 에너지 조건으로 팬텀 선량을 평가하고 Phase space data 통해 영상을 구현을 연구 하였습니다. 결 과:3D slicer를 이용한 CT 스캔 표면 데이터와 3D 광학 스캔 표면 데이터의 정량적 비교 분석에서 Hounsdroff distance는 Average 2.45 mm, Dice coefficient는 0.97로 나타나 CT 스캔 표면 데이터와 3D 스캔 표면 데이터가 거의 일치하는 것을 확인할 수 있었습니다. 또한 인체모형 팬텀 재현성 평가에서 인체모형 팬텀의 AP position, RAO position, LAO position 기준으로 팔이 있는 팬텀의 error distribution은 2 mm 오차 범위에서 평균 89.78%가, 4 mm 오차 범위에서 96.05%가 일치하는 것으로 나타났으며, 팔을 제외한 경우 error distribution은 2 mm 오차 범위에서 평균 96.48%가, 4 mm 오차 범위에서 99.45%가 일치하는 것으로 나타나 3D 광학 스캐너의 표면 데이터 재현성이 우수한 것으로 나타났습니다. 인체모형 팬텀을 이용한 3D 스캔 표면 데이터를 MCs에 적용하여 전신일반촬영 에너지 조건으로 팬텀선량 평가가 가능하며, Phase space data 통해 영상을 구현할 수 있다는 것을 확인 하였습니다. 결 론:본 연구에서 3D 광학 스캐너를 사용한 인체모형 팬텀 데이터 획득은 기존 CT scan 방식보다 간편하고 방사선 노출 없이 다양한 위치와 균일한 표면 구조의 정보를 정밀하게 얻을 수 있으며 MCs을 이용한 선량계산이 가능하며, Phase space data 통해 영상을 구현이 가능함에 따라 앞으로 일반촬영 분야의 활용과 선량평가에 매우 유용할 것으로 생각 됩니다. Purpose:The purpose of this work is to acquire surface data of anthropromophic phantom using 3D optical scanner instead of conventional computed tomography (CT) scanning methods and to compare the differences between existing CT scan surface data and 3D optical scan surface data. also it is to apply 3D optical scan surface data to Monte Carlo simulations (MCs) to study whether phantom dose evaluation and image implementation are possible under general shooting energy conditions. Targets and Methods:We suggest utilizing a 3D optical scanner when obtaining patient surface data for dose evaluation of normal photography. We obtained surface data of anthropromophic phantom instead of conventional CT scanning methods using 3D optical scanners (Go!SCAN SPARKTM, CREAFORM). The Meshlab program was used to sort and edit 3D scan surface data, and the CT scan surface data and 3D scan surface data differences were quantitatively compared using the 3D slicer program. The reproducibility of the dummy phantom was also evaluated using the 3D Scan Surface Data Analysis Program (VXelements, CREAFORM). Using the SpekCalc program, the X-ray beam energy spectrum was calculated and the X-ray beam was modeled using TOPAS, a Geant4-based MCs. We applied the anthropromophic phantom to MCs evaluate the phantom dose in X-ray energy conditions and study whether the image can be implemented through phase space data. Result:Quantitative comparative analysis of CT scan surface data and 3D optical scan surface data using 3D slicer showed that Hounsdroff distance was Average 2.45 mm and Dice coefficient was 0.97, indicating a close match between CT scan surface data and 3D scan surface data. In addition, the anthropromophic phantom reproducibility assessment shows that the error distribution of the phantom with arms by AP position, RAO position, and LAO position of the anthropromophic phantom averaged 89.78% in the 2 mm error range and 96.05% in the 4 mm error range. MCs using anthropromophic phantom confirmed that 3D scan surface data can be applied to Monte Carlo simulation to evaluate phantom dose under X-ray energy conditions through phase space data. Conclusion:In this study, anthropromophic phan tom data acquisition using 3D optical scanners is simpler than conventional CT scan methods, provides precise information of various locations and uniform surface structures without radiation exposure, enables dosing using MCs, and enables image implementation through phase space data.

Functional Screening of a Metagenomic Library Reveals Operons Responsible for Enhanced Intestinal Colonization by Gut Commensal Microbes

Yoon, Mi Young,Lee, Kang-Mu,Yoon, Yujin,Go, Junhyeok,Park, Yongjin,Cho, Yong-Joon,Tannock, Gerald W.,Yoon, Sang Sun American Society for Microbiology 2013 Applied and environmental microbiology Vol.79 No.12

<P>Evidence suggests that gut microbes colonize the mammalian intestine through propagation as an adhesive microbial community. A bacterial artificial chromosome (BAC) library of murine bowel microbiota DNA in the surrogate host <I>Escherichia coli</I> DH10B was screened for enhanced adherence capability. Two out of 5,472 DH10B clones, 10G6 and 25G1, exhibited enhanced capabilities to adhere to inanimate surfaces in functional screens. DNA segments inserted into the 10G6 and 25G1 clones were 52 and 41 kb and included 47 and 41 protein-coding open reading frames (ORFs), respectively. DNA sequence alignments, tetranucleotide frequency, and codon usage analysis strongly suggest that these two DNA fragments are derived from species belonging to the genus <I>Bacteroides</I>. Consistent with this finding, a large portion of the predicted gene products were highly homologous to those of <I>Bacteroides</I> spp. Transposon mutagenesis and subsequent experiments that involved heterologous expression identified two operons associated with enhanced adherence. <I>E. coli</I> strains transformed with the 10a or 25b operon adhered to the surface of intestinal epithelium and colonized the mouse intestine more vigorously than did the control strain. This study has revealed the genetic determinants of unknown commensals (probably resembling <I>Bacteroides</I> species) that enhance the ability of the bacteria to colonize the murine bowel.</P>

Microarray Analysis of Gene Expression Alteration in Human Middle Ear Epithelial Cells Induced by Asian Sand Dust

Go, Yoon Young,Park, Moo Kyun,Kwon, Jee Young,Seo, Young Rok,Chae, Sung-Won,Song, Jae-Jun Korean Society of Otorhinolaryngology-Head and Nec 2015 Clinical and Experimental Otorhinolaryngology Vol.8 No.4

<P><B>Objectives</B></P><P>The primary aim of this study is to evaluate the gene expression profile of Asian sand dust (ASD)-treated human middle ear epithelial cell (HMEEC) using microarray analysis.</P><P><B>Methods</B></P><P>The HMEEC was treated with ASD (400 µg/mL) and total RNA was extracted for microarray analysis. Molecular pathways among differentially expressed genes were further analyzed. For selected genes, the changes in gene expression were confirmed by real-time polymerase chain reaction.</P><P><B>Results</B></P><P>A total of 1,274 genes were differentially expressed by ASD. Among them, 1,138 genes were 2 folds up-regulated, whereas 136 genes were 2 folds down-regulated. Up-regulated genes were mainly involved in cellular processes, including apoptosis, cell differentiation, and cell proliferation. Down-regulated genes affected cellular processes, including apoptosis, cell cycle, cell differentiation, and cell proliferation. The 10 genes including <I>ADM</I>, <I>CCL5</I>, <I>EDN1</I>, <I>EGR1</I>, <I>FOS</I>, <I>GHRL</I>, <I>JUN</I>, <I>SOCS3</I>, <I>TNF</I>, and <I>TNFSF10</I> were identified as main modulators in up-regulated genes. A total of 11 genes including <I>CSF3</I>, <I>DKK1</I>, <I>FOSL1</I>, <I>FST</I>, <I>TERT</I>, <I>MMP13</I>, <I>PTHLH</I>, <I>SPRY2</I>, <I>TGFBR2</I>, <I>THBS1</I>, and <I>TIMP1</I> acted as main components of pathway associated with 2-fold down regulated genes.</P><P><B>Conclusion</B></P><P>We identified the differentially expressed genes in ASD-treated HMEEC. Our work indicates that air pollutant like ASD, may play an important role in the pathogenesis of otitis media.</P>

Studies on Isolation and Characterization of Benzoate - degrading Bacteria

Young Been Yoon,Jeong Gyu Kim,Eon Ho Choi 한국응용생명화학회(구 한국농화학회) 1994 한국응용생명화학회 학술발표회 Vol.1994 No.1

Changes in maternal health due to multiple-metal exposure: Changes in total cholesterol, triglyceride levels, and association between premature birth and gestational diabetes

( Soo-young Jung ),( Yoon-young Go ),( Young-ah You ),( Young Min Hur ),( Sunwha Park ),( Gain Lee ),( Young Ju Kim ) 대한산부인과학회 2023 대한산부인과학회 학술대회 Vol.109 No.-