Development of a New Zenoestrogen Screening System Using Fission Yeast Schizosaccharomyces pombe

Yoo, Eung Jae,Jang, Yeun Kyu,Kimm, Hyun Soo,Choi, Eun Sik,Park, Sang Dai Korean Society of Molecular Biology 2002 Molecules and cells Vol.13 No.1

The impact of caregiver’s role preference on decisional conflicts and psychiatric distresses in decision making to help caregiver’s disclosure of terminal disease status.

Yoo, Shin Hye,Yun, Young Ho,Kim, Kyoung-Nam,Lee, Jung Lim,PARK, Jeanno,Choi, Youn Seon,Lim, Yeun Keun,Kim, Samyong,Jeong, Hyun Sik,Kang, Jung Hun,Oh, Ho-Suk,Park, Jichan,Kim, Si-Young,Song, Hong-Suk,L American Society of Clinical Oncology 2017 Journal of clinical oncology Vol.35 No._suppl15

<P> 10106 </P><P> Background: A decision aid (DA) increases knowledge, decreases decisional conflicts and regrets and improves post-decision satisfaction, emotional distress. However, few DA trials have revealed whether decisional role preferences have an impact on patient-reported outcomes by decision making. The objective of this study was to investigate the impact of caregiver’s decisional role preference on decisional conflicts and psychiatric distresses in decision making. Methods: 406 of 444 caregivers of terminally ill cancer patients enrolled onto a previous trial determining the efficacy of the decision aid about disclosure of terminal disease status were included in this analysis. The analysis outcomes were change score of decisional conflicts using the Decision Conflict Scale (DCS) and depression and anxiety using the Hospital Anxiety and Depression Scale (HADS) at 1 and 3 months from baseline. Participants were divided into 4 groups: active caregiver who received DA (active-DA), active caregiver in control group (active-control), passive caregiver who received DA (passive-DA), and passive caregiver in control group (passive-control). Linear mixed model was conducted to find out the impact of caregiver’s decisional role preference on the DCS and the HADS. Results: Among 406 caregivers, 137 (33.7%) showed active role preference, and 269 (66.3%) showed passive role preference. In post-hoc analysis of adjusted differences of change scores between passive-DA and active-DA groups, non-significant differences were observed in DCS. However, at 3 months, change scores of HADS depression subscale increased as 4.43 (95% confidence interval (CI), 0.78-8.07; P< 0.007; effect size (ES) 0.71) and those of HADS anxiety subscales increased as 4.14 (95% CI, 0.37-7.91; P= 0.021; ES 0.61) in passive-DA group than in active-DA group, showing moderate to large difference. Conclusions: These findings suggest that information about decision making might be provided with tailored format for how much individual wish to involve in decision making. </P>

전신마취후 경미한 합병증에 대한 고찰

김완식,박동호,유희구,정연탁 대한마취과학회 1978 Korean Journal of Anesthesiology Vol.11 No.4

Recently, with the remarkable theoretical progress made in anesthesiology these years, reports are being made on the results of the study on the complications, minor or major, caused by previous general anesthesia. However, .the reports on incidence vary according to the reporters. According to Riding, the recent development of anesthesiology, anesthetic instruments, knowledge of physiology, anesthetics, induction agents and muscle relaxants are said to have reduced the incidence of complications to a great extent. Riding, Gold and Dyrberg report that the factors capable of influencing the incidence of complication, in the post-operative period include age, sex, premedicants for anesthetics, inhalation method of anesthetics, time of anesthesia, muscle relaxant, use of induction agent, acid-base imbalance and developed knowledge of physioloy, operation site, mental state of patients before operation, general condition, anesthetic technique, and. adequate selection of medicine. Working for Department of Anesthesiology, Hanyang University, from October 1976 to August 1977, we selected, out of the patients who received general anesthesia, 523 males and 372 females, totalling 895, who showed no abnormal symptoms in their respiratory systems, circulatory systems and metabolic systems and observed the incidence of complication in the light of sex, age and operation site, the factors supposed to affect the incidence. An hour prior to the general anesthesia, the patients were given intramuscular injection with premedicants atropine 0. 01 mg per kg, Valium 0. 2 mg or Demerol 1 mg per kg of body weight. For induction of anesthesia, Epontol 10 mg per kg and succinylcholine 1 mg per kg of body weight were injected in the veins, then ventilation was made for a minute with mask and then endotracheal tubes were inserted. and then a minimum amount of air was injected. into the cuff of the endotracheal tube, and the ventilation was done in a semi-closed system. During the period of maintenance, anesthesia was administered in a semi-closed system with 0. 5~l. 0% halothane, 3 L/min of nitrous oxide, 2 L/min of oxygen; and if need be, muscle relaxant was injected into the vein. In case a nondenolarizing agent was used in the course of maintenance, atropine 0. 5~1. 0 mg and neost igmine 2. 0~4. 0mg were injected into the vein for reversion at the recovery time. After the patient was completely recovered, the endotracheal tube was cautiously removed, so as not to give trauma to the throat. As for method of observations, 24 hours after the patient had recovered, we visited the patient in the ward, first observing the existence or nonexistence of incidence of minor complications, and then calculating the incidence by the distribution of sex, age and operation site. The outcome of the observation of the above results by statistics and by chi square test is as follows; 1) the incidence of complications after general anesthesia was high in females. 2) Age has not affected the incidence of complications. 3) The incidence of nausea was highest in the patients with abdominal operation. 4) The incidence of sore throat was highest in the head and neck patients. 5) The incidence of fever was highest in the abdomen patients. 6) The incidence of headache was highest in the head and neck patients.

소아 미추마취의 임상적 고찰

김완식,박동호,유희구,김영석,정연탁,김미연 대한마취과학회 1979 Korean Journal of Anesthesiology Vol.12 No.2

Many studies have cited occurrences of severe and long-lasting emotional disturbances of almost every kind after surgical procedures, particularly due to the sedatives or narcotics which were administered to control the post-operative pain. In order to control this post-operative pain, pediatric caudal analgesia was performed in 15 infants and children from the age 2 months to 6 years, who were to undergo lower abdomen, perineum and lower extremity surgery. They were induced with inhalation anesthoeia, using nitrous oxide, oxygen and halothane by mask before and during the block. Following the block, infants and children were turned to supine position and the initial concentration of halothane was reduced. All cases were given 10mg/kg of 1% solution of lidocaine. On examination of the level of the analgesia, 13 out of 15 cases had spread higher level than T₁0, with effective block for surgical procedures. The identification of the sacral cornu is easy and technical failureis lees in comparison than with the umbar epidural block for infants and children. Thus we can perform this block sucessfully for infants and children without any complications or accidents. It is especially helpful in the management of postoperative cases due to the effect in control of the postoperative pain. Thus it gives satistaction to their parents, nurses and doctors. Despite no follow-up study on postoperative emotional reactions, it was considered that there were minimal occurrences of emotional and behavioral sequelae following the hospital and surgical experiences.

보리새싹 함유 녹즙의 항산화력 분석에 관한 연구

유인식(In-Sik Yoo),백청목(Cheong-Mok Baek),정미연(Mi-Yeun Joung),권상철(Sang-Chul Kwon) 한국산학기술학회 2017 한국산학기술학회논문지 Vol.18 No.12

본 연구는 보리새싹 함유 녹즙의 항산화력 분석을 위한 목적으로 수행 하였다. 실험에 사용 된 시료는 충북 진천군 소재의 CSJ 회사로 부터 공급 받아서 실험에 사용하였다. Polyphenol, Flavonoid 함량 분석결과 어성초발효새싹녹즙은 1.075±0.174 mg/mL, 2.736±0.187 quercetin mg/mL, 유기민들레는 1.048±0.173 mg/mL, 2.685±0.245 quercetin mg/mL, 칸앤락 1.013±0.083 mg/mL, 3.624±0.237 quercetin mg /mL, 브로콜리혼합즙은 1.494±0.255 mg/mL, 3.826±0.286 quercetin mg/mL, 보리새싹은 1.043±0.220 mg/mL, 1.990±0.223 quercetin mg/mL의 폴리페놀과 플라보노이드 함량이 검출되었다. 항산화능 실험결과 DPPH에 대한 어성초발효새싹녹즙 전자공여능은 81.97%로 나타났으며, ABTS assay 방법에 의한 free radical 소거능을 측정한 결과 브로콜리혼합즙, 어성초발효새싹녹즙, 유기민들레, 칸앤락, 보리새싹 순으로 활성이 증가하는 것으로 나타났으나 Ascorbic acid 100 ㎍/mL와 비교할 때 그 효과가 비슷한 수준으로 나타났다. 통계처리는 SPSS24을 이용하여 일원배치분산분석(one-way ANOVA)을 실시하였으며, Duncan"s multiple range test를 실시하였다.(p<0.05) 향후 제품들의 기능성 부재료로 사용하여 제품화하면 건강기능성 음료로서의 가치가 있음을 나타내었다. This study was conducted to analyze the anti-oxidant activity of green juice containing barley sprouts. The specimens used in the experiments were supplied byCSJ company located in Jincheon-gun, Chungbuk province. Polyphenol and flavonoid was detected in Huttuynia cordata Thunb fermentation sprout juiceat 1.075±0.174 mg/mL and2.736±0.187 quercetin mg/mL, in organic dandelion at 1.048±0.173 mg/mL and 2.685±0.245 quercetin mg/mL, kan andRakat 1.013±0.083 mg/mL and 3.624±0.237 quercetin mg /mL, broccoli blending juice at 1.494±0.255 mg/mL and3.826±0.286 quercetin mg/mL, and barley sprouts at 1.043±0.220 mg/mL and1.990±0.223 quercetin mg/mL, respectively.Antioxidant activity testing revealed the electron donating ability of Huttuynia cordata Thunb fermentation sprout juice for DPPH was 81.97%. Moreover, the free radical scavenging ability measured by ABTS assay showed that the activity was increased in the order of broccoli blending juice,Huttuynia cordata Thunb fermentation sprout juice,organic dandelion,"kan andRak", barley sprout, and thatthe effects were similar to those of ascorbic acid at 100 ?g/mL. Statistical processing is using SPSS 24, analysis of distributed layout and Duncan"s multiple range (p<0.05). Therefore, the use of functional assistance material of products in the future indicates that it is worth a healthy functional drink.

Molecular Characterization of a Chinese Cabbage cDNA Encoding Thioredoxin-h that is Predominantly Expressed in Flowers

Lee, Seung-Sik,Lee, Kyun-Oh,Jung, Bae-Gyo,Chi, Yong-Hun,Yoo, Ji-Young,Lee, Ji-Yeun,Lee, Jung-Ro,Park, Soo-Kwon,Kang, Soon-Suk,Jang, Ho-Hee,Lee, Sang-Yeol Korean Society for Biochemistry and Molecular Biol 2001 Journal of biochemistry and molecular biology Vol.34 No.4

Even though three isotypes of thioredoxins (-f, -m and -h types) have been identified in a variety of plant cells, there are only a few reports on thioredoxin-h that were recently identified. In this study, a cDNA encoding a h-type of thioredoxin was isolated from a cDNA library of Chinese cabbage, and named here CTrx-h. An open reading frame of the gene contained a polypeptide of 133 amino acids with a conserved active center, WCGPC, which appeared in all of the thioredoxin proteins. A deduced amino acid sequence of the CTrx-h showed the highest sequence identity with those of Arabidopsis thioredoxin-h2 (75.2%) and thioredoxin-h5 (46.6%) proteins, but it shared a low sequence homology to other isotypes of plant thioredoxinm and thioredoxin-f. The CTrx-h protein that is expressed in E. coli represented not only an insulin reduction activity, but also electron transferring activity from NADPH to thioredoxin-dependent peroxidase. A genomic Southern blot analysis using the cDNA insert of CTrx-h revealed that the gene consisted of a small multigene family in Chinese cabbage genome. On the contrary to other thioredoxin-h proteins that were widely distributed in most tissues of the plant, the CTrx-h was predominantly expressed in flowers. The expression was very low in other tissues. The data of the Northern blot analysis suggests that the CTrx-h may have other functions in flower development or differentiation, in addition to its defensive role.

1996년 부산시에서 집단발생한 장티푸스 환자들로부터 분리한 Salmonella typhi의 PFGE양상, Phage형 및 항균제감수성

박미선,강연호,유정식,신영학,이복권,김호훈 대한감염학회 1997 감염 Vol.29 No.3

목적: 1996년 4월부터 6월사이 부산시 수영구에서 집단 발생한 장티푸스 환자로부터 분리된 장티푸스균 48주를 대상으로 phage형 시험 및 염색체 DNA를 제한효소로 처리 후 PFGE를 시행, 그 양상을 분석, 역학적 도구로서의 유용성을 검토하고 장티푸스 치료의 효율적 약제선택의 기초 자료로 제공하고자 항생제 감수성시험을 하였다. 방법: 항생제 감수성 시험은 장내세균 감수성 시험에 권장되고 있는 ampicillin외 14종의 항균제에 대하여 NCCLS 표준 디스크 확산법으로 수행하였다. phage형 시험은 영국 중앙 공중 보건 실험실(PHRL)에서 분양 받은 phage액을 사용하여 시험하였다. PFGE 시험은 장티푸스균 염색체 DNA를 제한효소 Spe Ⅰ으로 처리후 PFGE를 수행, 그 영동상을 관찰 분석하였다. 결과: 시험대상 장티푸스균 48주중 ampicillin의 14종의 항균제에 내성을 보인 균주는 없었다. phage형은 비분류주(untypable)가 44주였고 M1이 2주, 그리고 E1형이 2주였다. PFGE양상 분석결과 A group(47주) 및 B group(1주)의 2가지 group으로 분류되었으며 A group은 A1(42주), A2(1주), A3(3주) 및 A4(1주) 등 4가지 subgroup으로 분류되었다. A group의 각 subgroup사이의 유전적 유사도(F치)는 0.8-0.897으로 유사도가 높았으나 A group의 각 subgroup과 B group간의 F치는 0.20-0.258로 유전적 근연 관계가 낮았다. 결론: 1996년 4월부터 6월사이 부산시 수영구에서 집단 발생한 장티푸스 초발 환자는 장티푸스균이 분리된 환자를 대상으로 장티푸스균의 PFGE양상 분석 결과 발병일(1996년 4월 23일)이 가장 빠른 것으로 신고된 남성 환자로 추정되었다. 염색체 DNA를 Spe Ⅰ제한효소로 처리 PFGE를 수행, 그 양상을 분석하는 방법이 장티푸스 집단발생시 감염원 및 유행전파 경로 추적등 역학적 분석에 유용성이 큰 것으로 밝혀졌다. 본 실험에 사용된 장티푸스 분리균은 장내세균 약제 감수성 시험에 일반적으로 권장되고 있는 ampicillin외 14종의 항균제에 내성을 나타낸 균주는 검출되지 않았으나 항균제 남오용 및 해외로부터 약제 내성 장티푸스균의 유입으로 약제 내성 장티푸스 출현 감시에 능동적 대처가 요망된다. Background: During the period from April to June 1996, an outbreak of typhoid fever occurred in SuYoung-Ku, Pusan city. We performed the molecular epidemiological analysis and drug susceptibility test for 48 isolates of Salmonella typhi from the outbreak. Methods: Chromosomal DNA of S. typhi was digested with the restriction endonuclease Spe Ⅰ and the resulting restriction polymorphism was observed on pulsed field gel electrophoresis(PFGE). Phage typing was performed using Vi-phages, and antimicrobial susceptibility test was done by Disc diffusion method. Results: On phage typing, 2 isolates were M1 phage type, 2 E1 phage type and 44 untypable. The PFGE analysis on 14 to 16 chromosomal DNA restriction fragments ranged from 50Kb to 1000kb showed 2 different patterns of restriction fragments, divided into group A and B. Forty-seven isolates belonged to group A that were subgrouped into A1(42 strains), A2(1 strain), A3(3 strains), A4(1strain), and one strain was grouped into group B. in the F-value of genetic similarity of the epidemic S. typhi strains, there was low similarity(F value: 0.2-0.258) between group A strains and group B strains. However, the subgroup strains(A1-A4) showed high similarity(F value:0.8-0.897) each other. The drug susceptibility test showed susceptibility to ampicillin, carbenicillin, cephalothin, chloramphenicol, tobramycin, gentamycin, kanamycin, nalidixic acid, neomycin, polymyxin B, streptomycin, tetracycline, trime-thoprim-sulfamethoxazole, ciprofloxacin and norfloxacin. Conclusion: This study showed that phage typing and PFGE were very useful as a tool to investigate molecular analyses of epidemic S. typhi strains this study.

관절경하 원위 쇄골 절제술을 이용한 만성 제 2형 견봉쇄골 손상의 치료 - 6례에 대한 예비 연구 -

유연식,윤영곤,남일현,문기혁,Yoo Yeun-Sik,Yoon Young-Gon,Nam Il-Hyun,Moon Gi-Hyuk 대한관절경학회 2002 대한관절경학회지 Vol.6 No.2

목적 : 저자들은 만성 제 2형 견봉쇄골 관절 탈구에 시행한 관절경하 원위 쇄골 절제술의 효과를 알아보고자 하였다. 대상 및 방법 : 1998년 2월부터 2001년 1월까지 제 2형 견봉쇄골 관절 탈구의 6례를 대상으로 시행하였으며 추시기간은 평균 12개월이었다. 검사의 결과의 평가는 constant-Murray 기능평가법을 이용하였으며 술 전 constant score는 32점 이었다. 결과 : 임상평가 결과 constant score는 술 후 $3\~6$주에서 35점 이었고 3개월은 49점 그리고 6개월에서는 69점으로 증가되었고 전체 환자의 $83\%$에서 만족도를 보였다. 결론 : 만성 제 2형 견봉쇄골 탈구에서 시행한 관절경하 원위 쇄골 절제술은 수기가 쉽고 간단하여 개방성 방법을 통한 광범위한 인대 재건술 또는 오구 쇄골간 고정술에 앞서 시행할 수 있는 방법이라 사료된다. Purpose : To evaluate effectiveness of arthroscopic distal clavicle resection for chronic stage 2 acromioclavicular dislocation. Material: 6 patients who had failed to respond to physical therapy were evaluate more than 6 months prospectively, which were treated with arthroscopic distal clavicular resection from february 1998 to January 2001. Result : The median preoperative Constant score changed from 32 of 100 to 37 of 100 at 3 to 6 weeks, to 49 of 100 at 3 months and to 69 of 100 at 6months. Improvement was achived at mean follow up more than 6 months after surgery. Overal $83\%$ of patient were satisfied with this procedure. but the remained case was unsatisfied because symptom was not improved more than 6 months Conclusion : Arthroscopic distal clavicle resection is useful method to treatment for chronic stage 2 acromioclavicular dislocation.

Functional characterictics and breeding of new commmercial strains Pleurotus ferula using Di-Mon mating and physiological assay

Pyung Gyun Shin,Min-Ji Oh,Yeun-Lee Oh,Kab Yeul Jang,Won Sik Kong,Young Bok Yoo,Hyung-Jun Noh,Keun-Ki Kim,Gun-Do Kim 한국버섯학회 2017 버섯 Vol.21 No.2

There are three kinds of Pleurotus eryngii : P. eryngii var. eryngii (P. eryngii), P. eryngii var. ferula (P. ferula : A-Wei-Mo) P. eryngii var. nebrodensis (P. nebrodensis : Bai-Ling-Gu). We bred P. ferula using Di-Mon mating and physiological assay and selected as follow (1) ‘Beesan No.1’ produced high yields, (2) ‘Beesan No.2’ was excellent morphological shape and anti-adipogenic Activit, (3) ‘Ergo’ included high ergothioneine such as anto-oxidant material, (4) ‘GW10-45’ included highest ergothioneine such as anto-oxidant material, (5) ‘Maeksong’ included high ergothioneine and effect of anti-inflammatory. We suggest that there are able to food-medicine materials.

New Ferula mushrooms of inter-specific Pleurotus ferula x Pleurotus tuoliensis using cytoplasmic hybrid with microsatellite marker

Pyung Gyun Shin,Min-Ji Oh,Yeun-Lee Oh,Kab Yeul Jang,Won Sik Kong,Young Bok Yoo 한국버섯학회 2017 버섯 Vol.21 No.2

The Pleurotus tuoliensis is a mushroom named Chinese ‘Bai-Ling-Gu’ from species of Pleurotus nebrodensis. we are mated for selection of cytoplasmic hybrid by mitochondria microsatellite marker and the method of Mon-Mon mating between monokaryotic strains derived from Pleurotus ferula ASI 0629 (Beesan No.1) and Pleurotus tuoliensis ASI 0663 (Baekryung 20). The cytoplasmic hybrids were identified 8 strains contained nuclear DNA bands of 'Baekryung 20' and mitochondrial DNA of monokaryon strains derived from Pleurotus ferula ASI 0629 (beesan No.1). The KiMB-Plft-15-81 was shown the best cultural characteristics, selected to be a new cultivar and designed as 'Baekwhang'. The 'Baekwhang' cab be grown without an extra after-ripening period, can utilize bottle cultivation of Pleurotus eryngii. And also the ‘Seolwon’ is similar to the existing Pleurotus tuoliensis in shape and physiological characteristics, formed high stipe. We therefore expect that this new strains will substitutional goods of Pleurotus eryngii.