임의적 패킷 손실과 외란입력을 고려한 네트워크 제어 시스템의 H<SUB>∞</SUB> 제어기 설계

이태희(Tae H. Lee),박주현(Ju H. Park),권오민(Oh-Min Kwon),이상문(Sang-Moon Lee) 대한전기학회 2013 전기학회논문지 Vol.62 No.8

This paper considers the H∞ control problem for networked control systems(NCSs). In order to solve the problem which comes from discontinuous control signal in NCSs, an approach that discontinuous control signals treat time-varying delayed continuous signals is applied to achieve H∞ stability of NCSs. In addition, randomly occurring packet losses and disturbances are considered by introducing stochastic variables with Bernoulli distribution. Based on Lyapunov stability theory, a new stability condition is obtained via linear matrix inequality formulation to find the H∞ controller which achieves the mean square stability of NCSs. Finally, the proposed method is applied to a numerical example in order to show the effectiveness of our results.

피부감작성에 있어 Local lymph node의 반응성 연구

이종권,박재현,김형수,정승태,엄준호,황인창,장은정,윤소미,남기택,허용,오혜영 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

알레르기설 접촉 괴부염은 다양한 화학물질에 의해 유포될 수 있다. 화학물질이 피부 감작성을 알 킬 수 있는지 여부는 전통적으로 기니픽을 사용한 등물모겔이 이용되어 쏜으나, 최근에는 마우스를 이용한 local Iymph node assily tLLNA) 발법 개발이 요구되고 있떠 본 연구는 focal Iymph not assa)·의 시험방법을 개발하고, 감작을 일으키는 기전을 파악하고자 하였다. 콕헝물질로는 패표적 접촉 알러전인 DffCB(2,4-dinitrochlorobenzone), 호홉 알러젠인 fDl(toluene diisocyanate), 약한 알러젠인Cf.(o-kerplcinamaldehl,do:4, 강한 자극물질인 SLS(sodum lauryl sulfate)를 사용하였다. 각 시험물질 2sr4를 적절한 농도로 6-8주령의 암컷 Balb/c 마우스의 귀의 배측에 3일간 도포한 후 5일째에 부검하여 이개림프절, 귀의 변화 등을 관찰하였다. DNCB, TDI를 도포한 마우스의 이개 림프절의 중량은 대조군에 비해 증가하였으며, 고농도의 HCA를 투여한 마우스에서의 림프절 중량도 증가하였다. SLS를 투여한 군의 림프걸치 중량은 저농도에서는 변화가 없었으나, 고농도에서는 중량이 증가하였다. 꼬리정맥에 3H-thymidine을 투여하여 림프절의 증식정도를 파악한 결과, BNCB를 도포한 군에서는 프절의 증식지수(Stimulation 1.Idex, Sl)가 용량별로 20.3, 24.6, 27.5로 관찰되었으며, TDI군에서는 각 19.0, 29.T, 42.3으로 관찰되첬고 HCA군에서는 각각 3.8, 9.7, 19.8로 관찰되었다. sLs군에서는 0.8 3.7, 6.6으로 관찰되었다. 이잖끈 결과 fH-thynudine을 이용한 피부 감작성 평가는 기니픽을 대체할수 있는 방법으로 _평가되었다. 그러나 방사선이용이라는 단점과 강한 자극성물질에서의 위양성 반응이 관찰되어 BrdU(Eromodeoklpuddine) 면역조직차학기법으로 시험한 결과 비방사선법도 이용 가능함을 알 수 있었다. 또는 TDI를 처리한 마우스의 림프절에서 cytoklne의 발현을 RT-PCR로 측정한 결과 IL-2 IL-4, U-10과 INf-r의 mRNA발현이 증가하였으며, HCA를 처리한 경우에는 토-4, IL-10. INf-γ mRNA의 발현이 증가 하였다. 또한 B리CB의 경우에는 U-4의 발현이 증가하였다. 그러나, sLs의 경우에는 IL-?, IL-4, 0--10 과 rNF-r꼭 발현이 증가되지 않았다. 이러한 결과는 LLNA를 이용한 c번okine의 n)교NA발현이 유용싼 측정 지포중 하나가 될 수 있을 것으로 생각되며. 특히 IL-4의 발현정도의 증가는 알레르기 유발성을 평가할 유용한 생체지표의 하나가 될 수 있을 것으로 생각되어진다. Allergic contact dermatitis (skin sensitization) may be caused by a wide variety of chemicals. A murine local lymph node assay(LLNA) has been developed as an alternative to guinea pig models for assessing the contact sensitization potential of chemical. First, the objective of this study was to evaluate allergenecity of chemicals by LLNA. Secondly, we aimed to investigate the possibility of development of non-radio isotopic endpoint for LLNA using immunohistochemistry. Thirdly, we aimed to analyze cytokine mRNA expression of draining lymph node cell in mice exposed to chemical allergen and irritant. In this study, contact allergen, dinitrochlorobenzone (DNCB), respiratory allergen toluene diisocyanate (TDI) and moderate allergen, α-hexylcinnamaldehyde (HCA) were used as positive chemicals and the irritant, sodium lauryl sulfate(SLS) also used as reference chemical. The stimulation index (SI) of lymph node cell in the mice treated with allergens. DNCB, TDI, and HCA were more increased dose-dependently compared to vehicle control by ^(3)H-thymidine uptake. And SI of lymph node cell in strong irritant, SLS were also increased dose-dependently compared to control. The BrdU(Bromodeoxyuridine) LI of lymph node in DNCB and TDI were dramatically increased campared to that of control. However, the LI of lymph node in SLS were not significantly increased compared to vehicle control. This data represents that BrdU LI of lymph node could be one of useful method for screening for irritant and allergen. The expression of IL-2, IL-4, IL-10 and IFN-γ mRNA was increased in draining auricular lymph node cell of the mice treated with TDI by RT-PCR. The level of IL-4, IL-10 and IFN-γmRNA was increased in mice treated with HCA. The expression of IL-4 was increased in lymph node cell of mice treated with DNCB. However, the level of IL-2, IL-4, IL-10 and IFN-γ in lymph node cell of mice treated with SLS was not increased. These results suggest the measurement for increase in level of IL-4 mRNA expression could by one of the method for screening the allergenic potential.

Extended Dissipative Analysis for Neural Networks With Time-Varying Delays

Lee, Tae H.,Myeong-Jin Park,Park, Ju H.,Oh-Min Kwon,Sang-Moon Lee IEEE 2014 IEEE transactions on neural networks and learning Vol.25 No.10

<P>In this brief, an extended dissipativity analysis was conducted for a neural network with time-varying delays. The concept of the extended dissipativity can be used to solve for the H<SUB>∞</SUB>, L<SUB>2</SUB> - L<SUB>∞</SUB>, passive, and dissipative performance by adjusting the weighting matrices in a new performance index. In addition, the activation function dividing method is modified by introducing a tuning parameter. Examples are provided to show the effectiveness and less conservatism of the proposed method.</P>

Hospital-based Influenza Morbidity and Mortality (HIMM) Surveillance for A/H7N9 Influenza Virus Infection in Returning Travelers

Song, Joon Young,Noh, Ji Yun,Lee, Jacob,Woo, Heung Jeong,Lee, Jin Soo,Wie, Seong-Heon,Kim, Young Keun,Jeong, Hye Won,Kim, Shin Woo,Lee, Sun Hee,Park, Kyung-Hwa,Kang, Seong Hui,Kee, Sae Yoon,Kim, Tae H KOREAN ACADEMY OF MEDICAL SCIENCE 2018 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.33 No.7

<P>Since 2013, the Hospital-based Influenza Morbidity and Mortality (HIMM) surveillance system began a H7N9 influenza surveillance scheme for returning travelers in addition to pre-existing emergency room (ER)-based influenza-like illness (ILI) surveillance and severe acute respiratory infection (SARI) surveillance. Although limited to eastern China, avian A/H7N9 influenza virus is considered to have the highest pandemic potential among currently circulating influenza viruses. During the study period between October 1st, 2013 and April 30th, 2016, 11 cases presented with ILI within seven days of travel return. These patients visited China, Hong Kong, or neighboring Southeast Asian countries, but none of them visited a livestock market. Seasonal influenza virus (54.5%, 6 among 11) was the most common cause of ILI among returning travelers, and avian A/H7N9 influenza virus was not detected during the study period.</P>

Genetically engineered fibroblasts with antigen-presenting capability:Efficient induction of an antigen-specific cytotoxic T-lymphocyte response and protection against tumor development in vivo

Kim, Tae S .,Chung, Su W .,Kim, Seung H .,Kang, Bok Y .,Hwang, Seung Y .,Lee, Jae W . 전남대학교 약품개발연구소 2000 약품개발연구지 Vol.9 No.1

BLK mouse fibroblasts (H-2^b) were genetically engineered to express costimulatory B7.1 and interleukin-2 (BLK/IL2/B7.1). The BLK/IL2/B7.1 cells were then pulsed with an ovalbumin (OVA) epitope as a model antigen (Ag) (BLK/IL2/B7.1/OVA), and tested for the induction of OVA-specific cytotoxic T lymphocytes (CTLs) in C57BL/6 mice (H-2^b). The genetically engineered fibroblasts lacking one or two of three factors (interleukin-2, B7.1, and OVA) were constructed and used as controls. Immunization with the BLK/IL2/B7.1/OVA cells induced strong cytotoxic activities against OVA-expressing EL4 (EG7) tumor cells, but not against other H-2^b tumor cells, such as EL4, C1498 and B16F1 cells. The magnitude of the cytotoxic response in mice with the BLK/IL2/B7.1/OVA cells was significantly higher than the response in mice immunized with any other cell constructs. CD8^+ T cells with OVA-specific cytotoxic activities were predominant in mice immunized with the BLK/IL2/B7.1/OVA cells. Furthermore, immunization with the BLK/IL2/B7.1/OVA cells significantly prolonged the survival of mice, compared with any other cell constructs, when the mice were challenged with EG7 tumor cells at 2 weeks postimmunization. Induction of antitumoral CTL immunity by the BLK/IL2/B7.1/OVA cells was independent of host Ag-presenting cells and of CD4^+ T-cell and natural killer 1.1^+ cell help. These results suggest that fibroblasts can be genetically modified to efficient Ag-presenting cells for the induction of an Ag-specific CTL response.

Characterization of Siberian wild rye grass EsHsp16.9 Gene and Their Expression under Various Environmental Conditions

Sang-Hoon Lee,Ki-Yong Kim,Hee Chung Ji,Tae Young Hwang,Ki-Won Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Small heat shock proteins (Hsps) are one of most conserved molecular chaperones that protect stress-inducible denaturation of substrates in living organisms. Small Hsps consist of a large subfamily categorized by subcellular localization ranging in size from 12 to 40 kDa. Here, we identified and characterized a small Hsp 16.9 gene (EsHsp16.9) from Siberian wild rye (Elymus sibiricus L.). EsHsp16.9 is a 456-bp cDNA with an open reading frame predicted to encode a 151-amino acid protein. It possesses a conserved ɑ-crystallin domain, which is a unique domain for small Hsps; shares high sequence similarity with cytosolic class I small Hsps among the small Hsp subfamily in Arabidopsis; and is close (96% similarity) to small Hsp in wheat. Northern blot analysis showed that EsHsp16.9 transcripts were enhanced by heat, drought, arsenate, methyl viologen, and H2O2 treatments. Moreover, we expressed and purified recombinant EsHsp16.9 proteins in Escherichia coli to confirm its activity as a molecular chaperone. We found that recombinant EsHsp16.9 exhibits effective molecular chaperone activity, as determined by inhibition of thermal aggregation of malate dehydrogenase (MDH), which is broadly used as a model substrate.

비혈연간 동종골수이식에 의해 치료된 중증 재생불량성빈혈 1예

김현중,이홍기,이방훈,이창근,이종태,정현식,김원석,윤성수,강원기,박근칠,김대식,고영혜,박찬형 대한조혈모세포이식학회 1997 대한조혈모세포이식학회지 Vol.2 No.1

비혈연간 동종골수이식은 혈연간 동종골수이식이 불가능한 환자에서 기존의 면역역제요법으로 효과가 없었던 경우나 재발한 경우에 적응증이 될수 있다. 이식 거부와 이식편대숙주병의 발생이 골수 이식의 성공률을 감소시키므로, 보다 강력한 conditioning regimen의 사용과 T cell이 제거된 골수를 사용하는 것이 필요하다. 기존의 high dose corticosteroid나 cyclosporine에 대하여 불응성인 이식편대숙주병의 치료를 위해서 ATG와 Mycophenolate mofetil (Cellcept®) 의 사용이나 Tacrolimus, Methotrexate와 같은 다른 면역억제제치료를 고려해 볼 수 있다. It is known that allogenic bone marrow transplantation is the most effective treatment for aplastic anemia. However, this treatment is only applicable to a minority of patients because the proportion of patients who have HLA-matched siblings about 25% to 30% of all the aplastic anemia patients. In the case of the absence of HLA-matched donor, unrelated allogenic bone marrow transplantation is considered. However, (because of severe acute and chronic GVHD and graft failure) the success rate of this approach is only 20~30%. Recently, it was reported that increased immunosuppressive condition combined with T cell depletion of the marrow graft would result in greater success. There was no case of successfully treated aplastic anemia patient by unrelated allogenic bone marrow transplantation in Korea. Here, we report a case of severe aplastic anemia successfully treated with unrelated allogenic bone marrow transplantation.

Siberian Sturgeon Oocyte Extract Induces Epigenetic Modifications of Porcine Somatic Cells and Improves Developmental Competence of SCNT Embryos

Kim, So-Young,Kim, Tae-Suk,Park, Sang-Hoon,Lee, Mi-Ran,Eun, Hye-Ju,Baek, Sang-Ki,Ko, Yeoung-Gyu,Kim, Sung-Woo,Seong, Hwan-Hoo,Campbell, Keith H.S.,Lee, Joon-Hee Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.2

Somatic cell nuclear transfer (SCNT) has generally demonstrated that a differentiated cell can convert into a undifferentiated or pluripotent state. In the SCNT experiment, nuclear reprogramming is induced by exposure of introduced donor nuclei to the recipient cytoplasm of matured oocytes. However, because the efficiency of SCNT still remains low, a combination of SCNT technique with the ex-ovo method may improve the normal development of SCNT embryos. Here we hypothesized that treatment of somatic cells with extracts prepared from the germinal vesicle (GV) stage Siberian sturgeon oocytes prior to their use as nuclear donor for SCNT would improve in vitro development. A reversible permeability protocol with $4{\mu}g/mL$ of digitonin for 2 min at $4^{\circ}C$ in order to deliver Siberian sturgeon oocyte extract (SOE) to porcine fetal fibroblasts (PFFs) was carried out. As results, the intensity of H3K9ac staining in PFFs following treatment of SOE for 7 h at $18^{\circ}C$ was significantly increased but the intensity of H3K9me3 staining in PFFs was significantly decreased as compared with the control (p<0.05). Additionally, the level of histone acetylation in SCNT embryos at the zygote stage was significantly increased when reconstructed using SOE-treated cells (p<0.05), similar to that of IVF embryos at the zygote stage. The number of apoptotic cells was significantly decreased and pluripotency markers (Nanog, Oct4 and Sox2) were highly expressed in the blastocyst stage of SCNT embryos reconstructed using SOE-treated cells as nuclear donor (p<0.05). And there was observed a better development to the blastocyst stage in the SOE-treated group (p<0.05). Our results suggested that pre-treatment of cells with SOE could improve epigenetic reprogramming and the quality of porcine SCNT embryos.

Microstructure and Mechanical Properties of Twin-Roll Strip Cast Mg Alloys

Park, Sung S.,Bae, Geun Tae,Lee, Jung G.,Kang, Dae H.,Shin, Kwang Seon,Kim, Nack J. Scientific.Net 2007 Materials Science Forum Vol.539-543 No.1

<P>Development of wrought Mg alloys, particularly in sheet form, is essential to support the growing interest for lightweight components in the automotive industry. However, development of Mg alloy sheets has been quite slow due to the complexity of sheet production originated from limited deformability of Mg. In this respect, twin-roll strip casting, a one-step processing of flat rolled products, can be an alternative for the production of Mg alloy sheets. In this study, AZ31 and experimental ZM series alloys are twin-roll strip cast into 2 mm thick sheets. The microstructure of the as-cast AZ31 alloy sheet consists of columnar zones near the roll side and equiaxed zones in the mid-thickness region. On the other hand, as-cast ZM series alloy sheets show equiaxed dendritic structure through the thickness of sheet. These alloys were subjected to various thermo-mechanical treatments and their tensile properties were evaluated. Twin-roll strip cast AZ31 alloy in H24 condition has equivalent yield and tensile strengths with similar ductility compared to commercial ingot cast AZ31-H24 alloy, indicating that twin-roll strip casting is a viable process for the fabrication of Mg alloy sheets. The experimental ZM series alloys have a large volume fraction of fine dispersoid particles in the microstructure, resulting from the beneficial effect of twin-roll strip casting on microstructural refinement. It has been shown that the experimental ZM series alloys have superior tensile properties compared to commercial ingot cast AZ31-H24 alloy, suggesting the possibility of the development of new wrought Mg alloy sheets by twin-roll strip casting.</P>

99mTc-RBC 간신티스캔을 이용한 간내 공간점유병소의 감별진단

이종태,이도연,유형식,김기황,박창윤,박찬희 대한핵의학회 1987 핵의학 분자영상 Vol.21 No.2

39 patients with focal hepatic lesions were evaluated by Tc-RBC liver scan. The diagnosis of focal hepatic lesions were made by percutaneous needle biopsy, angiography, surgery, or clinical courses. Thses diagnoses included 24 cases of hemangioma, 7 hepatomas, 6 rnetastatic disease, 1 abscess, and 1 cyst. 19 hemangiomas showed focal hot activity on delayed static planar images. 3 small deep seated hemangiomas were diagnosed by SPECT that would have been missed by planar images alone. 21arge hemangiomas had no radioisotope uptake within the lesions on delayed images and at surgery cavernous hemangioma with thrombosis, calcification, and fibrosis were found. For hepatic heman- giomas in our series, the sensitivity was 91.7% and the specificity was 100%. The remaining 15 patients including hepatomas, metastatic lesions, cyst and abscess showed cold defect on delayed blood pool images. It is concluded that (99)^Tc-RBC liver scan should be the choice of primary diagnostic procecure for clinically suspected hepatic hemangioma since its inexpensive, non-invasive, and readly available.